Slide dyeing device for rapid on-site evaluation

By designing a miniaturized slide staining device, and using limiting units and driving units to achieve automated slide staining, the existing device's large volume and high dyeing liquid consumption are solved, the dyeing efficiency and quality are improved, and it is suitable for rapid on-site evaluation during endoscopic diagnosis and treatment.

CN223078009UActive Publication Date: 2025-07-08UNIV OF SHANGHAI FOR SCI & TECH +1
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Patent Information

Application Number
CN202422189133.X
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-09-06
Publication Date
2025-07-08
Estimated Expiration
2034-09-06

AI Technical Summary

Technical Problem

The existing slide staining device is large in size and consumes a lot of dyeing fluid. It is not suitable for rapid on-site evaluation in diagnostic interventional operations, and there is a risk of sample contamination, making it difficult to achieve automation.

Method used

A miniaturized slide dyeing device is designed, including a box, a dyeing component and an automatic liquid supply unit. The limiting unit, a driving unit and a peristaltic pump are used to realize automatic slide dyeing. Through the coordination of the limiting guide rail and the driving unit, the dyeing time and dye usage are controlled to reduce sample contamination.

Benefits of technology

It realizes automated slide staining with miniaturization and low dye consumption, improves dyeing efficiency and quality, and is suitable for rapid on-site evaluation during endoscopic diagnosis and treatment.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model relates to a slide dyeing device for rapid on-site evaluation. The slide dyeing device comprises a box body, a box body cover, a dyeing component and an automatic liquid supply unit, wherein the dyeing component and the automatic liquid supply unit are positioned in the box body; the dyeing assembly comprises a dyeing workbench, a limiting unit, a connecting unit and a driving unit; a through hole allowing the dyeing workbench to extend out is formed in the front face of the box body, when the limiting guide rail is located at the first working position, the dyeing workbench extends out of the through hole, and the two breast board hinges are parallel to each other; when the limiting guide rail is located at the second working position, the two breast board hinges are opened by a set angle, and the five-head dropper is aligned to one end of the dyeing workbench in the vertical direction; when the limiting guide rail is located at the third working position, the dyeing workbench is located below the air drying unit, and the two breast board hinges are parallel to each other. Compared with the prior art, the utility model has the advantages of small volume, less dye consumption and controlled dyeing time, and improves the dyeing efficiency and the dyeing quality.
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Description

Technical Field

[0001] The utility model relates to the technical field of medical clinical inspection, in particular to a slide staining device for rapid on-site evaluation. Background Art

[0002] Rapid on-site evaluation (ROSE) is an extremely fast cytological judgment technique accompanying diagnostic interventional procedures. It can quickly interpret the puncture specimens obtained by endoscopic ultrasound physicians, evaluate whether the sufficiency of the specimens can meet the diagnostic requirements, provide timely on-site feedback, decide whether to repeat the puncture operation or change the puncture path to obtain more and high-quality specimens, improve the diagnostic rate, reduce unnecessary punctures, and thus reduce the occurrence of operation-related complications.

[0003] Clinically, the Diff-Quik staining method is most commonly used to stain the puncture smears for rapid on-site evaluation. The reagents used are Diff-Quik Solution A, Diff-Quik Solution B, and clear water for cleaning. The main staining steps are to soak the prepared slide in Diff-Quik Solution A for 10 to 30 seconds, then immerse the slide in Diff-Quik Solution B for staining for about 20 to 40 seconds, rinse with clear water, and after drying, it can be sent to an optical microscope for pathological diagnosis.

[0004] Although the existing slide staining components can stain slides in large quantities, they occupy a large space, consume a large amount of staining solution, are not suitable for rapid on-site evaluation during diagnostic interventional procedures, and most of the existing devices use the dipping technique, with a high possibility of sample contamination, affecting the sample quality. At present, most hospitals in China are difficult to achieve automatic slide staining in the rapid on-site evaluation of diagnostic interventional procedures. Summary of the Utility Model

[0005] The purpose of the utility model is to overcome the defects of the above-mentioned existing technologies and provide a slide staining device for rapid on-site evaluation. The utility model has a small volume, less dye consumption, and controlled staining time, improving the staining efficiency and staining quality.

[0006] The purpose of the utility model can be achieved by the following technical solutions:

[0007] The utility model provides a slide staining device for rapid on-site evaluation, which is a small-volume device that can be placed in the operating room to achieve automatic slide staining during endoscopic diagnosis and treatment. The use of this device is beneficial to reducing the dye consumption, controlling the staining time, and improving the staining efficiency and staining quality.

[0008] The utility model provides a slide staining device for rapid on-site evaluation, including: a box body, a box cover, and a staining component and an automatic liquid supply unit located inside the box body;

[0009] The dyeing component includes a dyeing workbench, a limiting unit, a connecting unit, and a driving unit; the limiting unit and the driving unit are fixedly connected through the connecting unit, and the dyeing workbench is fixedly connected to the limiting unit;

[0010] The automatic liquid supply unit includes a dye solution bottle, a clear water tank, a peristaltic pump unit, and a five - head dropper. The peristaltic pump unit includes multiple peristaltic pumps. Each dye solution bottle is connected to a peristaltic pump, and the clear water tank is connected to a peristaltic pump, and then they converge into the five - head dropper; when replacing the dye solution or adding clear water, open the box cover, and the dye bottle and the clear water tank can be taken out to achieve the addition and replacement of the liquid.

[0011] The limiting unit includes a sliding plate and a limiting guide rail. An active latch is provided on the sliding plate and is matched with the limiting guide rail; the limiting guide rail has a first working position, a second working position, and a third working position: a through - hole for the dyeing workbench to extend out is provided on the front of the box body. When the limiting guide rail is in the first working position, the dyeing workbench extends out from the through - hole, and the two fence hinges are parallel to each other; when the limiting guide rail is in the second working position, the two fence hinges open at a set angle, and the five - head dropper is vertically aligned with one end of the dyeing workbench; when the limiting guide rail is in the third working position, the dyeing workbench is located below the air - drying unit, and the two fence hinges are parallel to each other.

[0012] Further, the connecting unit includes two fence hinges. The two fence hinges are rotatably connected, and the fence hinges are respectively fixedly connected to the limiting unit and the driving unit.

[0013] Further, the driving unit includes: a slider, a lead screw, a slide rail, and a stepping motor. The slider is arranged on the slide rail and can slide along the slide rail. The stepping motor drives the lead screw to achieve linear movement; through the linear movement of the slider, the vertical lifting of the sliding plate is realized, so as to achieve the purpose of increasing the included angle between the two fence hinges.

[0014] Further, the dyeing workbench includes a quick - dyeing table, a glass - loading table, and two spring pieces. Five glass - slide grooves are provided on the glass - loading table and are fixed on the quick - dyeing table through the two spring pieces.

[0015] The limiting unit is fixedly connected to the quick - dyeing table, so that while the driving unit drives the dyeing workbench to slide on the slide rail, a certain angle can exist between the dyeing workbench and the driving unit.

[0016] Further, the quick - dyeing table is provided with a liquid diversion inclined plane, and liquid outlets are provided on both sides of the quick - dyeing table. Through the diversion inclined plane and the liquid outlets, the concentration and collection of the discharged waste liquid are realized, which is convenient for subsequent cleaning and treatment.

[0017] Further, when the limit guide rail is in the first working position, the staining workbench extends out from the through hole, and the staining workbench is parallel to the bottom surface of the box body. Five glass slides to be processed are sequentially placed on the glass slide table and wait for staining.

[0018] Further, the peristaltic pump unit includes: a first peristaltic pump, a second peristaltic pump, and a third peristaltic pump;

[0019] When the limit guide rail is in the second working position, the five-head dropper is vertically aligned with one end of the glass slide table, and there is an included angle between the staining workbench and the bottom surface of the box body so that the liquid can flow along the inclined plane;

[0020] The first peristaltic pump works to push the dye solution A to the five-head dropper, and it vertically drops onto the glass slide table. The staining workbench is an inclined plane in the second working position, and due to the gravity factor, the dye solution flows through the entire glass slide; then the stepping motor of the driving unit drives the staining workbench to move to the third working position and stand still for a period of time to complete the staining of the dye solution A. The horizontal third working position can save dyes.

[0021] Further, after the staining of the dye solution A is completed, it returns to the second working position, and the second peristaltic pump starts to work to push the dye solution B to the five-head dropper, and it vertically drops onto the glass slide table. The dye solution flows through the entire glass slide; the stepping motor of the driving unit drives the staining workbench to move to the third working position to complete the staining of the dye solution B, that is, the staining process ends; it returns to the second working position again, and the third peristaltic pump works to spray clear water from the five-head dropper to flush out the residual and redundant dye solution on the glass slide to realize the cleaning process.

[0022] Further, an air drying unit and a touch display screen are provided on the box body. The air drying unit includes two fans placed above the slide rail. After the staining and cleaning steps are completed, the fans are started to realize the drying treatment of the glass slides.

[0023] Further, ventilation holes are provided on the box body to realize the circulation of the air inside the box.

[0024] The working principle of the present utility model is as follows:

[0025] The staining workbench extends out from the through hole, that is, it is in the first working position. The user sequentially places five glass slides to be processed on the glass slide table and waits for staining. Operate the touch display screen to start staining. The staining workbench moves towards the inside of the box body along the slide rail, and at the same time, the staining workbench is lifted by a certain angle until it reaches the second working position. The first peristaltic pump pumps out the dye solution from the five-head dropper to soak the glass slide with the dye solution A, and then moves to the third working position and stands still for a period of time. After the staining of the dye solution A is completed, it returns to the second working position. The staining of the dye solution B is completed by the same principle. The third peristaltic pump works to realize the flushing of the glass slide. The air drying unit is started to complete the drying of the glass slide, and the staining workbench returns to the first working position, and the processed glass slides are taken out.

[0026] Compared with the prior art, the utility model has the following advantages:

[0027] The utility model is small in volume, uses less dye, has a controlled dyeing time, and improves the dyeing efficiency and quality. The inclination and horizontal conversion of the dyeing workbench are realized through the limiting guide rail. When inclined, the dye solution infiltrates the glass slide, and when horizontal, the dyeing is static, saving the amount of dye used and controlling the dyeing time. Only one stepping motor is used to drive the device to work normally, reducing the volume of the device. During the endoscopic diagnosis and treatment process, automatic glass slide dyeing can be realized, improving the dyeing efficiency and quality. Description of the Drawings

[0028] Figure 1 is a schematic diagram of the overall structure of the utility model;

[0029] Figure 2 is a schematic diagram of the structure of the box cover of the utility model;

[0030] Figure 3 is a schematic diagram of the structure of the dyeing assembly of the utility model;

[0031] Figure 4 is a schematic diagram of the structure of the dyeing workbench of the utility model;

[0032] Figure 5 is a schematic diagram of the structure of the connection unit of the utility model;

[0033] Figure 6 is a schematic diagram of the automatic liquid supply unit of the utility model;

[0034] Figure 7 is a schematic diagram of the first working position of the utility model;

[0035] Figure 8 is a schematic diagram of the second working position of the utility model;

[0036] Figure 9 is a schematic diagram of the third working position of the utility model.

[0037] Reference numerals: 1 - box body, 11 - through hole, 12 - ventilation hole, 2 - box cover, 3 - dyeing assembly, 31 - dyeing workbench, 311 - quick dyeing table, 312 - glass slide stage, 313 - spring piece, 314 - diversion inclined plane, 315 - liquid outlet, 32 - limiting unit, 321 - slide plate, 322 - limiting guide rail, 323 - movable latch, 33 - connection unit, 331 - railing hinge, 34 - driving unit, 341 - slider, 342 - lead screw, 343 - slide rail, 344 - stepping motor, 4 - automatic liquid supply unit, 41 - dye solution bottle, 42 - water tank, 43 - peristaltic pump unit; 431 - first peristaltic pump, 432 - second peristaltic pump, 433 - third peristaltic pump, 44 - five - head dropper, 5 - air - drying unit, 6 - touch display screen. Detailed implementation mode

[0038] The present utility model will be described in detail below with reference to the accompanying drawings and specific embodiments. Features such as component models, material names, connection structures, control methods, algorithms, etc. that are not clearly described in this technical solution are regarded as common technical features disclosed in the prior art.

[0039] Embodiment 1

[0040] This embodiment provides a slide staining device for rapid on-site evaluation, as shown in Figure 1 、 2 、3, 4, 5, 6, and includes: a box body 1, a box body cover 2, and a staining assembly 3 and an automatic liquid supply unit 4 located inside the box body 1;

[0041] The staining assembly 3 includes a staining workbench 31, a limiting unit 32, a connecting unit 33, and a driving unit 34; the limiting unit 32 and the driving unit 34 are fixedly connected through the connecting unit 33, and the staining workbench 31 is fixedly connected to the limiting unit 32;

[0042] The automatic liquid supply unit 4 includes a dye solution bottle 41, a clear water tank 42, a peristaltic pump unit 43, and a five-head dropper 44. The peristaltic pump unit 43 includes a plurality of peristaltic pumps. Each dye solution bottle 41 is connected to a peristaltic pump, and the clear water tank 42 is connected to a peristaltic pump, and then converges into the five-head dropper 44; when replacing the dye solution or adding clear water, open the box body cover 2, and the dye bottle 41 and the clear water tank 42 can be taken out to realize the addition and replacement of the liquid.

[0043] As shown in Figure 7 、 8 、9, the limiting unit 32 includes a slide plate 321 and a limiting guide rail 322. An active latch 323 is provided on the slide plate 321 and cooperates with the limiting guide rail 322; the limiting guide rail 322 has a first working position, a second working position, and a third working position: a through hole 11 for the staining workbench 31 to extend out is provided on the front surface of the box body 1. When the limiting guide rail 322 is in the first working position, the staining workbench 31 extends out from the through hole 11, and the two fence hinges 331 are parallel to each other; when the limiting guide rail 322 is in the second working position, the two fence hinges 331 open at a set angle, and the five-head dropper 44 is vertically aligned with one end of the staining workbench 31; when the limiting guide rail 322 is in the third working position, the staining workbench 31 is located below the air drying unit 5, and the two fence hinges 331 are parallel to each other.

[0044] In the detailed implementation mode, the connecting unit 33 includes two fence hinges 331. The two fence hinges 331 are rotatably connected, and the fence hinges 331 are respectively fixedly connected to the limiting unit 32 and the driving unit 34.

[0045] In a specific embodiment, the driving unit 34 includes: a slider 341, a lead screw 342, a slide rail 343, and a stepping motor 344. The slider 341 is arranged on the slide rail 343 and can slide along the slide rail 343. The stepping motor 344 drives the lead screw 342 to achieve linear movement; through the linear movement of the slider 341, the vertical lifting of the slide plate 321 is realized, so as to achieve the purpose of increasing the included angle of the two fence hinges 331.

[0046] In a specific embodiment, the dyeing workbench 31 includes a rapid dyeing table 311, a glass slide platform 312, and two spring pieces 313. Five glass slide grooves are arranged on the glass slide platform 312 and are fixed on the rapid dyeing table 311 by the two spring pieces 313.

[0047] The limiting unit 32 is fixedly connected to the rapid dyeing table 311, so that when the driving unit 34 drives the dyeing workbench 31 to slide on the slide rail 343, a certain angle can exist between the dyeing workbench 31 and the driving unit 34.

[0048] In a specific embodiment, the rapid dyeing table 311 is provided with a liquid diversion inclined plane 314, and liquid outlets 315 are arranged on both sides of the rapid dyeing table 311. Through the diversion inclined plane 314 and the liquid outlets 315, the concentration and collection of the discharged waste liquid are realized, which is convenient for subsequent cleaning treatment.

[0049] In a specific embodiment, when the limiting guide rail 322 is in the first working position, the dyeing workbench 31 extends out of the through hole 11, and the dyeing workbench 31 is parallel to the bottom surface of the box body 1. Five glass slides to be processed are sequentially placed on the glass slide platform 312 and wait for dyeing.

[0050] In a specific embodiment, the peristaltic pump unit 43 includes: a first peristaltic pump 431, a second peristaltic pump 432, and a third peristaltic pump 433;

[0051] When the limiting guide rail 322 is in the second working position, the five - head dropper 44 is vertically aligned with one end of the glass slide platform 312, and the dyeing workbench 31 forms an angle with the bottom surface of the box body 1 so that the liquid can flow along the inclined plane;

[0052] The first peristaltic pump 431 works to push the dye solution A to the five - head dropper 44, and it vertically drops onto the glass slide platform 312. The dyeing workbench 31 is an inclined plane in the second working position, and due to the gravity factor, the dye solution flows through the entire glass slide; then the stepping motor 344 of the driving unit 34 drives the dyeing workbench 31 to move to the third working position and stand still for a period of time to complete the dyeing of the dye solution A. The horizontal third working position can save dyes.

[0053] In the specific implementation manner, after the staining with dye solution A is completed, it returns to the second working position, and the second peristaltic pump 432 starts to work, pushing the dye solution B to the five-headed dropper 44, which vertically drops onto the glass slide table 312, and the dye solution flows through the entire glass slide; the stepping motor 344 of the driving unit 34 drives the staining workbench 31 to move to the third working position to complete the staining with dye solution B, that is, the staining process ends; it returns to the second working position again, and the third peristaltic pump 433 works to push the clear water to spray out from the five-headed dropper 44 to wash out the residual and excess dye solution on the glass slide, realizing the cleaning process.

[0054] In the specific implementation manner, an air drying unit 5 and a touch display screen 6 are provided on the box body 1. The air drying unit 5 includes two fans, which are placed above the slide rail 343. After the staining and cleaning steps are completed, the fans are started to realize the drying treatment of the glass slides. The touch display screen 6 is arranged on the box body cover 2, as Figure 2 shown.

[0055] In the specific implementation manner, ventilation holes 12 are opened on the box body 1 to realize the circulation of the air inside the box.

[0056] The working principle of the present utility model is as follows:

[0057] The staining workbench 31 extends out from the through hole 11, that is, it is in the first working position. The user sequentially places five glass slides to be processed on the glass slide table 312 and waits for staining. Operate the touch display screen 66 to start staining. The staining workbench 31 moves towards the inside of the box body 1 along the slide rail 343, and at the same time, the staining workbench 31 is lifted by a certain angle until it reaches the second working position. The first peristaltic pump 431 pumps out the dye solution from the five-headed dropper 44 to soak the glass slides with dye solution A, moves to the third working position, and stands still for a period of time. After the staining with dye solution A is completed, it returns to the second working position. The staining with dye solution B is completed by the same principle. The third peristaltic pump 433 works to realize the flushing of the glass slides. The air drying unit 5 is started to complete the drying of the glass slides, and the staining workbench 31 returns to the first working position to take out the processed glass slides.

[0058] The components not described in detail in this embodiment are all existing components that can be purchased from public channels.

[0059] The above description of the embodiments is for the convenience of those of ordinary skill in the art to understand and use the utility model. Those skilled in the art can obviously make various modifications to these embodiments easily and apply the general principles described herein to other embodiments without creative labor. Therefore, the present utility model is not limited to the above embodiments, and the improvements and modifications made by those skilled in the art according to the disclosure of the present utility model without departing from the scope of the present utility model should be within the protection scope of the present utility model.

Claims

1. A slide staining device for rapid on-site assessment, characterized in that Comprising: A box body (1), a box body cover (2), and a dyeing assembly (3) and an automatic liquid supply unit (4) located inside the box body (1); The dyeing assembly (3) includes a dyeing workbench (31), a limiting unit (32), a connecting unit (33), and a driving unit (34); the limiting unit (32) and the driving unit (34) are fixedly connected through the connecting unit (33), and the dyeing workbench (31) is fixedly connected to the limiting unit (32); The automatic liquid supply unit (4) includes a dye solution bottle (41), a clear water tank (42), a peristaltic pump unit (43), and a five - head dropper (44). The peristaltic pump unit (43) includes a plurality of peristaltic pumps. Each dye solution bottle (41) is connected to one peristaltic pump, and the clear water tank (42) is connected to one peristaltic pump, and then converges into the five - head dropper (44); The limiting unit (32) includes a sliding plate (321) and a limiting guide rail (322). An active latch (323) is arranged on the sliding plate (321) and cooperates with the limiting guide rail (322); the limiting guide rail (322) has a first working position, a second working position, and a third working position: a through - hole (11) for the dyeing workbench (31) to extend out is opened on the front surface of the box body (1). When the limiting guide rail (322) is in the first working position, the dyeing workbench (31) extends out from the through - hole (11); when the limiting guide rail (322) is in the second working position, the five - head dropper (44) is vertically aligned with one end of the dyeing workbench (31); when the limiting guide rail (322) is in the third working position, the dyeing workbench (31) is located below the air - drying unit (5).

2. The slide staining device for rapid on-site evaluation according to claim 1, wherein The connecting unit (33) includes two railing hinges (331). The two railing hinges (331) are rotatably connected, and the railing hinges (331) are respectively fixedly connected to the limiting unit (32) and the driving unit (34).

3. The slide staining device for rapid on-site evaluation according to claim 1, characterized in that, The driving unit (34) includes: a slider (341), a lead screw (342), a slide rail (343), and a stepping motor (344). The slider (341) is arranged on the slide rail (343), and the slider (341) can slide along the slide rail (343). The stepping motor (344) drives the lead screw (342) to achieve linear movement; through the linear movement of the slider (341), the vertical lifting of the sliding plate (321) is realized.

4. A slide staining device for rapid on-site evaluation according to claim 1, characterized in that, The dyeing workbench (31) includes a rapid - dyeing table (311), a glass - slide table (312), and two spring pieces (313). Five glass - slide grooves are arranged on the glass - slide table (312) and are fixed on the rapid - dyeing table (311) through the two spring pieces (313).

5. A slide staining device for rapid on-site evaluation according to claim 4, characterized in that, The rapid - dyeing table (311) is provided with a liquid guiding inclined plane (314), and liquid outlet ports (315) are arranged on both sides of the rapid - dyeing table (311). Through the guiding inclined plane (314) and the liquid outlet ports (315), the concentration and collection of the discharged waste liquid are realized, which is convenient for subsequent cleaning treatment.

6. The slide staining device for rapid on-site evaluation according to claim 4, wherein When the limiting guide rail (322) is in the first working position, the dyeing workbench (31) extends out from the through - hole (11), and the dyeing workbench (31) is parallel to the bottom surface of the box body (1). Five glass slides to be processed are sequentially placed on the glass - slide table (312) and wait for dyeing.

7. A slide staining device for rapid on-site evaluation according to claim 4, characterized in that, The peristaltic pump unit (43) includes: a first peristaltic pump (431), a second peristaltic pump (432), and a third peristaltic pump (433); When the limit guide rail (322) is in the second working position, the five - headed dropper (44) is vertically aligned with one end of the glass slide table (312), and the staining workbench (31) forms an angle with the bottom surface of the box body (1) so that the liquid can flow along the inclined plane; The first peristaltic pump (431) operates to push the dye solution A to the five - headed dropper (44), and it vertically drops onto the glass slide table (312). The staining workbench (31) is an inclined plane in the second working position, and due to the gravity factor, the dye solution flows through the entire glass slide. Then, the stepping motor (344) of the driving unit (34) drives the staining workbench (31) to move to the third working position and stand still for a period of time to complete the staining of the dye solution A. The horizontal third working position can save dyes.

8. A slide staining device for rapid on-site evaluation according to claim 7, characterized in that, After the staining of the dye solution A is completed, it returns to the second working position, and the second peristaltic pump (432) starts to operate to push the dye solution B to the five - headed dropper (44), and it vertically drops onto the glass slide table (312). The dye solution flows through the entire glass slide. The stepping motor (344) of the driving unit (34) drives the staining workbench (31) to move to the third working position to complete the staining of the dye solution B, that is, the staining process ends. Then it returns to the second working position again, and the third peristaltic pump (433) operates to push the clear water to spray out from the five - headed dropper (44) to wash out the residual and excess dye solution on the glass slide, realizing the cleaning process.

9. A slide staining device for rapid on-site evaluation according to claim 1, characterized in that, An air - drying unit (5) and a touch display screen (6) are provided on the box body (1).

10. A slide staining device for rapid on-site evaluation according to claim 1, characterized in that, Ventilation holes (12) are provided on the box body (1) to realize the circulation of the air inside the box.