Array laser fiber detection fluorescence labeling biological instrument

Abstract

An array laser optical fiber detection fluorescence labeling biological instrument comprises an array laser light source, an array quartz optical fiber sensor and a dot matrix biological labeling plate, and is characterized in that dot matrix micro-pit slots which are arranged according to a rule are prepared on the surface of transparent plastic or glass of the dot matrix biological labeling plate, and detected biological standard samples are arranged in the micro-pit slots; soaking with a biological reagent solution containing fluorescence labeled particles, cleaning, and binding the biological standard sample and the fluorescence labeled particles in the micro-pit slot after reacting and combining; the dot matrix biomarker plate with the fluorescent particles is excited by the array laser light source, the fluorescent particles emit light, and the light is transmitted, detected and recorded by the optical fiber. According to the dot matrix biomarker plate, various markers and different types of labeled same-color fluorescent particles can be stored on one dot matrix biomarker plate, independent interpretation or simultaneous rapid and accurate interpretation can still be achieved in detection, and the dot matrix biomarker plate can be widely applied to bacterial virus detection of medical treatment, food and the like.

Description

Technical Field

[0001] The present invention belongs to the technical field of laser fiber biological detection. Background Art

[0002] Existing array biological detection technologies mostly involve excitation light scanning and counting imaging with a CCD or a photomultiplier tube. Their drawback is that they cannot independently excite and interpret each unit. Even for multi-channels, they cannot independently adjust the excitation signal and interpretation for each unit. Detection of multiple bacteria and viruses usually requires multi-color labeled particles or multiple types of reagent kits, resulting in low interpretation efficiency and prone to errors.

[0003] An array laser fiber detection fluorescence-labeled biological instrument of the present invention includes: an array laser light source, an array quartz fiber sensor, and a dot matrix biological marker plate. It is characterized in that: the dot matrix biological marker plate is prepared with regularly arranged dot matrix micro-pit grooves on the surface of transparent plastic or glass. The micro-pit grooves are provided with biological samples to be detected. After being soaked with a biological reagent solution containing fluorescence-labeled particles and then cleaned, the biological samples react and bind with the fluorescence-labeled particles and are bound in the micro-pit grooves; the dot matrix biological marker plate with fluorescent particles is excited by the laser in the array laser light source, the fluorescent particles emit light, and the light is detected and recorded by optical fiber conduction.

[0004] The array laser fiber detection fluorescence-labeled biological instrument of the present invention can store multiple markers on one dot matrix biological marker plate. Different types of markers are labeled with the same color fluorescent particles, and can still be independently interpreted or simultaneously and respectively interpreted quickly and accurately during detection, and can be widely used for detection of bacteria and viruses in medical treatment, food, etc. Summary of the Invention

[0005] An array laser fiber detection fluorescence-labeled biological instrument includes: an array laser light source 1, an array quartz fiber sensor 3, and a dot matrix biological marker plate 2. It is characterized in that: the dot matrix biological marker plate is prepared with regularly arranged dot matrix micro-pit grooves 8 on the surface of transparent plastic or glass. The micro-pit grooves are provided with biological samples to be detected. After being soaked with a biological reagent solution containing fluorescence-labeled particles and then cleaned, the biological samples react and bind with the fluorescence-labeled particles and are bound in the micro-pit grooves; the dot matrix biological marker plate with fluorescent particles is excited by the laser 4 in the array laser light source, the fluorescent particles emit light, and the light is detected and recorded by optical fiber 7 conduction.

[0006] The array laser light source in the present invention is an infrared semiconductor laser or a blue semiconductor laser. Each laser light source is restricted within its own independent laser light source control area 5 and is independently adjusted. The array laser light source, the micro-pit grooves of the dot matrix biological marker plate, and the array quartz fiber sensor form a one-to-one superposition structure. The blue semiconductor laser corresponds to the biological marker using ultraviolet fluorescent materials. The infrared semiconductor laser corresponds to the biological marker using rare earth up-conversion fluorescent materials. The array laser light source, the array quartz fiber sensor, and the dot matrix biological marker plate are jointly enclosed in a light-tight housing.

[0007] One end of the quartz fiber in the array quartz fiber sensor in the present invention is equipped with a convex lens and is inlaid and placed under the micro-pit grooves of the dot matrix biological marker plate. The dot matrix biological marker plate can be extracted and replaced. The end point 6 of the quartz fiber is aligned with the micro-pit grooves of the biological marker plate. The array quartz fibers are bundled, woven, or twisted into an optical fiber cable according to a pattern; the regular arrangement of the numerous array fibers can reduce misalignment recognition. The braiding machine and the stranding machine need to be specially manufactured to prevent the quartz fiber from breaking. The other end of the quartz fiber in the array quartz fiber sensor is connected to a photomultiplier tube or a semiconductor photoelectric sensor.

[0008] The diameter of the micro-pit grooves of the dot matrix biological marker plate in the present invention is 0.1 - 2 microns. The micro-pit grooves are formed by burning with a laser on the surface of transparent glass or plastic, using ultraviolet laser or infrared laser to burn the grooves on the glass or plastic. Antibody-labeled particles can be set in the micro-pit grooves. First, the initial brightness of the fluorescent particles is detected and recorded, and then the biological marker plate is placed in the antigen solution and taken out for cleaning. After the antigen-antibody binding, the fluorescence intensity of the label changes. The present invention can perform quantitative analysis. The antigen is fixed and labeled in the micro-pit grooves of the biological marker plate, soaked in a solution of fluorescent particles containing antibodies, then cleaned, and placed in the instrument for detection, and the target can be quantitatively analyzed in the array.

[0009] The fluorescent labeled particles in the present invention are up-conversion fluorescent particles or ultraviolet-emitting fluorescent particles, and their surfaces are connected with bioactive molecules. The labeled different biological detection materials are numbered and fixed in the micro-pit grooves of the dot matrix biological marker plate according to a pattern one by one. The independent movement and placement of the fluorescent particles with different labels can be achieved by using devices such as semiconductor chip movement.

[0010] The surface of the quartz fiber in the present invention is coated or extruded and wrapped with a light-shielding protective layer. The light-shielding protective layer uses an enameling device to wrap a light-shielding coating on the surface of the fiber, and then uses an extruder and a casting machine to wrap a plastic protective layer. After the array quartz fibers are bundled, stranded, and woven, there will be no mutual influence between the fibers.

[0011] The fluorescent labeled particles in the present invention are up-conversion fluorescent particles, and the array laser light source is a 980nm infrared semiconductor laser, and the up-conversion fluorescent particles produce green light emission.

[0012] The fluorescent labeled particles in the present invention are ultraviolet fluorescent particles, the array laser light source is a 470 nm semiconductor laser, the power of each independent excitation light source in the array can be adjusted, and the ultraviolet fluorescent particles generate red light emission. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 is a schematic structural diagram of an array laser fiber detecting fluorescent labeled biological instrument

[0014] In the drawings of the present invention: 1 is an array laser light source, 2 is a dot matrix biological marker plate, 3 is an array quartz fiber sensor, 4 is a laser, 5 is a laser light source control area, 6 is a quartz fiber end point, 7 is a fiber optic, and 8 is a micro-pit groove. DETAILED DESCRIPTION OF THE INVENTION

[0015] An array laser fiber detecting fluorescent labeled biological instrument, which includes: an array laser light source 1, an array quartz fiber sensor 3, and a dot matrix biological marker plate 2. The characteristics are: the dot matrix biological marker plate is prepared with regularly arranged dot matrix micro-pit grooves 8 on the surface of transparent plastic or glass. The micro-pit grooves are provided with biological samples to be detected. After being soaked in a biological reagent solution containing fluorescent labeled particles and then cleaned, the biological samples react and bind with the fluorescent labeled particles and are bound in the micro-pit grooves; the dot matrix biological marker plate with fluorescent particles is excited by the laser 4 in the array laser light source, and the inorganic fluorescent particles generate light emission, which is transmitted and detected by the fiber optic 7. The inorganic fluorescent particles have the advantage of stability and will not change under laser irradiation.

[0016] The array laser light source in the present invention is an infrared semiconductor laser or a blue light semiconductor laser. Each laser light source is limited within its own independent laser light source control area 5 and is independently adjustable. The array laser light source, the micro-pit grooves of the dot matrix biological marker plate, and the array quartz fiber sensor form a one-to-one superposition structure. The blue light semiconductor laser corresponds to the use of ultraviolet fluorescent materials for biological markers. The infrared semiconductor laser corresponds to the use of rare earth up-conversion fluorescent materials for biological markers. The array laser light source, the array quartz fiber sensor, and the dot matrix biological marker plate are jointly enclosed in a light-proof housing.

[0017] One end of the quartz fiber in the array quartz fiber sensor in the present invention is embedded under the micro-pit grooves of the dot matrix biological marker plate. The diameter of the quartz fiber is 0.1 - 0.5 mm. The quartz fiber end point 6 is provided with an optical lens to amplify the optical signal and align it with the micro-pit grooves of the biological marker plate. The array quartz fibers are regularly bundled and woven or twisted into an optical fiber cable; the regular arrangement of the array of optical fibers can reduce misalignment recognition, and the braiding machine and the stranding machine need to be specially made to prevent the quartz fiber from breaking. The other end of the quartz fiber in the array quartz fiber sensor is connected to a photomultiplier tube or a semiconductor photoelectric sensor.

[0018] The diameter of the micro-pit grooves on the dot matrix bio-marker plate in the present invention is 0.1 - 2 microns. The micro-pit grooves are formed by burning on the surface of transparent glass or plastic using a laser. An ultraviolet laser engraving machine or an infrared laser marking machine is used to burn the pit grooves on the glass or plastic, so that they can be prepared quickly in batches.

[0019] The fluorescently labeled particles in the present invention are up-conversion fluorescent particles or inorganic fluorescent particles that emit ultraviolet light. Their surfaces are connected to bioactive molecules and can be stored for a long time. Different bio-detection materials are labeled one by one and placed in the micro-pit grooves of the dot matrix bio-marker plate according to a rule. The independent movement and placement of the fluorescent particles with different markers can be achieved by using equipment such as semiconductor chip movement. Inorganic fluorescent materials have the advantages of stability and weather resistance.

[0020] The surface of the quartz optical fiber in the present invention is coated or extruded and wrapped with a light-shielding protective layer. The light-shielding protective layer is formed by wrapping a light-shielding coating on the optical fiber surface using enameling equipment. The coating thickness of the light-shielding coating is 1 micron, and commercially available light-shielding coatings can be used. Then, a plastic protective layer is wrapped using an extruder and a casting machine. Plastics such as PVC and PE are used. After the array of quartz optical fibers is bundled, twisted, and braided, there will be no mutual influence between the optical fibers. The braided optical fibers can be clearly arranged corresponding to the matrix numbers.

[0021] The fluorescently labeled particles in the present invention are inorganic up-conversion fluorescent particles. The surfaces of the fluorescent particles are silanized and then connected to bioactive substances. The array laser light source is a 980 nm infrared semiconductor laser, and the up-conversion fluorescent particles emit green light.

[0022] The fluorescently labeled particles in the present invention are inorganic ultraviolet fluorescent particles. The silanization treatment of the surfaces of the fluorescent particles is a conventional biotechnology treatment, and then they are connected to bioactive substances. The array laser light source is a 470 nm semiconductor laser, and the power of each array of independent excitation light sources can be adjusted. The ultraviolet fluorescent particles emit red light.

Claims

1. An array laser fiber detection fluorescence-labeled biological instrument, which comprises: Array laser light source, array quartz optical fiber sensor, and dot matrix biological marker plate, characterized in that: the array laser light source corresponds one-to-one with the array quartz optical fiber sensor; the dot matrix biological marker plate is prepared with regularly arranged dot matrix micro-pit grooves on the surface of transparent plastic or glass, and the micro-pit grooves are provided with biological samples to be detected. After being soaked in a biological reagent solution containing fluorescently labeled particles and then cleaned, the biological samples react and bind with the fluorescently labeled particles and are bound in the micro-pit grooves; the dot matrix biological marker plate with fluorescent particles is excited by the array laser light source, the fluorescent particles emit light, and are detected and recorded by optical fiber conduction.

2. The array laser fiber detection fluorescence-labeled biological instrument according to claim 1, characterized in that: The array laser light source is an infrared semiconductor laser or a blue semiconductor laser, and is independently adjustable; the micro-pit grooves of the array laser light source and the dot matrix biological marker plate and the array quartz optical fiber sensor form a one-to-one superimposed structure; the array laser light source, the array quartz optical fiber sensor, and the dot matrix biological marker plate are jointly enclosed in a light-tight housing.

3. The array laser fiber detecting fluorescence-labeled biological instrument according to claim 1, characterized in that: One end of the quartz optical fiber in the array quartz optical fiber sensor is equipped with a convex lens and is placed below the micro-pit grooves of the dot matrix biological marker plate, and the array quartz optical fibers are bundled, braided or twisted into an optical fiber cable regularly; the other end of the quartz optical fiber in the array quartz optical fiber sensor is connected to a photomultiplier tube or a semiconductor photodetector.

4. The array laser fiber detection fluorescence-labeled biological instrument according to claim 1, characterized in that: The diameter of the micro-pit grooves of the dot matrix biological marker plate is 0.1 - 2 microns.

5. An array laser fiber detection fluorescence-labeled biological instrument according to claim 1, characterized in that: The fluorescently labeled particles are upconversion fluorescent particles or ultraviolet-emitting fluorescent particles, and their surfaces are connected to bioactive molecules.

6. The array laser fiber detection fluorescence-labeled biological instrument according to claim 1, characterized in that: The surface of the quartz optical fiber is coated or extruded and wrapped with a light-shielding protective layer, and there is no influence after the array quartz optical fibers are bundled, twisted and braided.

7. An array laser fiber detecting fluorescence-labeled biological instrument according to claim 1, characterized in that: The fluorescently labeled particles are upconversion fluorescent particles, and the array laser light source is a 980nm infrared semiconductor laser.

8. The array laser fiber detection fluorescence-labeled biological instrument according to claim 1, wherein: The fluorescently labeled particles are ultraviolet fluorescent particles, and the array laser light source is a 470nm semiconductor laser.

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