Wide-channel micro-fluidic chip sealing clamp

Abstract

The utility model discloses a wide-channel micro-fluidic chip sealing clamp, which belongs to the technical field of micro-fluidic-flow microimaging measurement accessories and comprises an upper cover plate, a lower plate, a side end face fastener, a straight-through pipe, a horizontal fastening screw and a vertical fastening screw. According to the utility model, the problem that the wide-channel micro-fluidic chip is difficult to seal is solved through a series of transition extrusion seals, and the condition that the traditional micro-fluidic chip is easy to block is solved.

Description

Technical Field

[0001] The utility model relates to the technical field of microfluidic-flow cytometry microscopic imaging accessories, and particularly relates to a sealing fixture for a wide-channel microfluidic chip. Background Art

[0002] Plankton, as the bottom organisms in the water ecosystem, are diverse in species, large in quantity, and sensitive to their habitats. Their community structure and abundance are indicators for evaluating the water ecological status. Currently, manual microscopy is still the main method for plankton species identification, but this method requires experienced identification personnel, has a long detection cycle, and cannot solve the problems of large-scale and high-frequency monitoring in ecological surveys. With the rapid development of micro-nano processing technology, microfluidic technology has gradually come into the view of researchers. Combining microfluidic technology with microscopic imaging technology is a way to quickly obtain images of algal cells. However, microfluidic chips have the phenomena of miniaturization and easy blockage, and wide-channel microfluidic chips are difficult to seal. Summary of the Invention

[0003] To solve the above technical problems, the utility model provides a sealing fixture for a wide-channel microfluidic chip.

[0004] To achieve the above utility model purpose, the utility model provides the following scheme:

[0005] A sealing fixture for a wide-channel microfluidic chip includes an upper cover plate, a lower plate, side-end fasteners, a straight-through pipe, horizontal fastening screws, and vertical fastening screws;

[0006] The upper cover plate is arranged above the lower plate in a matching manner. A groove for placing a microfluidic chip is formed in the lower plate. The side-end fasteners are arranged in the groove of the lower plate to fasten the microfluidic chip from both sides. Matching screw holes and vertical fastening screws are formed on the upper cover plate and the lower plate in the vertical direction to connect and fasten the microfluidic chip. Screw holes and horizontal fastening screws are formed on the lower plate in the horizontal direction to squeeze the side-end fasteners from both sides to fasten the microfluidic chip. Through holes for communicating the sample inlets at both ends of the microfluidic chip are formed on both sides of the lower plate. The straight-through pipe is arranged in the through holes to connect with the sample inlets of the microfluidic chip.

[0007] In the above technical solution, the height of the side-end fastener is 0.5 - 1 mm higher than the end face of the lower plate of the microfluidic chip fixture.

[0008] In the above technical solution, the number of the vertical fastening screws is 4, which are distributed on both sides of the microfluidic chip, 2 on each side.

[0009] In the above technical solution, the number of the horizontal fastening screws is 4, which are distributed on both sides of the lower plate, 2 on each side.

[0010] In the above technical solution, the material of the side end face fastener is polytetrafluoroethylene.

[0011] In the above technical solution, the fixture further includes a pressure head, a Luer connector and a Teflon hard tube. The pressure head is connected to one end of the straight-through tube. The Luer connector is connected to the pressure head, and the Teflon hard tube is connected to the Luer connector.

[0012] Beneficial effects:

[0013] The utility model discloses a wide-channel microfluidic chip sealing fixture. The utility model solves the difficult sealing problem of the wide-channel microfluidic chip through a series of transitional extrusion seals, and solves the problem of easy blockage of the traditional microfluidic chip. Description of the drawings

[0014] In order to make the purpose, technical solution and beneficial effects of the utility model clearer, the utility model provides the following drawings:

[0015] Figure 1 It is a schematic diagram of the overall assembly structure of a wide-channel microfluidic chip sealing fixture of the utility model.

[0016] Figure 2 It is a schematic cross-sectional structure diagram of a wide-channel microfluidic chip sealing fixture of the utility model.

[0017] Wherein: 1, microfluidic chip; 2, side end face fastener; 3, lower plate; 4, straight-through tube; 5, pressure head; 6, Luer connector; 7, Teflon hard tube; 8, horizontal fastening screw; 9, upper cover plate; 10, vertical fastening screw. Detailed implementation manners

[0018] Next, the preferred embodiments of the utility model will be described in detail with reference to the drawings.

[0019] Embodiment

[0020] As Figure 1 and Figure 2 shown, a wide-channel microfluidic chip sealing fixture includes an upper cover plate 9, a lower plate 3, a side end face fastener 2, a straight-through tube 4, a horizontal fastening screw 8 and a vertical fastening screw 10;

[0021] The upper cover plate 9 is disposed above the lower plate 3 in a matching manner. A groove for placing the microfluidic chip 1 is formed in the lower plate 3. The side end surface fastener 2 is disposed in the groove of the lower plate 3 for fastening the microfluidic chip 1 from both sides. Matching screw holes are formed in the upper cover plate 9 and the lower plate 3 in the vertical direction, and vertical fastening screws 10 are used to connect and fasten the upper cover plate 9 and the lower plate 3 to fasten the microfluidic chip 1. Screw holes and horizontal fastening screws 8 are formed in the lower plate 3 in the horizontal direction for squeezing the side end surface fastener 2 from both sides to fasten the microfluidic chip 1. Through holes for communicating the two ends of the sample inlet of the microfluidic chip 1 are formed on both sides of the lower plate 3. The straight-through tube 4 is disposed in the through hole for connecting with the sample inlet of the microfluidic chip 1.

[0022] In this embodiment, the height of the side end surface fastener 2 is 0.5 mm higher than the end surface of the lower plate 3.

[0023] In this embodiment, the number of the vertical fastening screws 10 is 4, which are distributed on both sides of the microfluidic chip 1, 2 on each side.

[0024] In this embodiment, the number of the horizontal fastening screws 8 is 4, which are distributed on both sides of the lower plate 3, 2 on each side.

[0025] In this embodiment, the material of the side end surface fastener 2 is polytetrafluoroethylene.

[0026] In this embodiment, the fixture further includes a pressure head 5, a Luer connector 6 and a Teflon hard tube 7. The pressure head 5 is connected to one end of the straight-through tube 4. The Luer connector 6 is connected to the pressure head 5. The Teflon hard tube 7 is connected to the Luer connector 6.

[0027] The assembly process and working principle of a wide-channel microfluidic chip sealing fixture in the above embodiment are as follows:

[0028] The microfluidic chip 1 is coupled with microscopic imaging. Considering the imaging quality, the glass thickness at both the upper and lower ends of the microfluidic chip 1 is 1.5 mm, and the size of the wide channel is 5 mm × 0.05 mm. The lower plate 3 of the microfluidic chip fixture is placed at the bottom. After the side-end fasteners 2 are installed on both sides of the fluid inlet at the side end of the microfluidic chip 1, they are installed in the bottom groove of the lower plate 3 of the microfluidic chip fixture; the height of the side-end fasteners 2 is 0.5 mm higher than the end face of the lower plate 3 of the microfluidic chip fixture. Further, the material of the side-end fasteners 2 is polytetrafluoroethylene; the upper cover plate 9 of the microfluidic chip fixture is installed above the lower plate 3 of the microfluidic chip fixture and the side-end fasteners 2, and is fixed on the lower plate 3 of the microfluidic chip fixture by four vertical fastening screws 10. During the tightening process, the side-end fasteners 2 are squeezed to complete the sealing of the upper and lower end faces of the microfluidic chip 1; the horizontal fastening screws 8 complete the sealing of the side ends of the sample inlets at both ends of the microfluidic chip 1 by squeezing the side-end fasteners 2 through the two horizontal fastening screws 8 of the lower plate 3 of the microfluidic chip fixture; the straight-through tube 4 completes the sealing of the sample inlets at both ends of the microfluidic chip 1 by squeezing the side-end fasteners 2 through the threaded ports of the lower plate 3 of the microfluidic chip fixture. The Teflon hard tube 7 passes through the Luer connector 6 and is squeezed into the press head 5, and the Luer connector 6 is screwed into the straight-through tube 4 to squeeze the Teflon hard tube 7 and the press head 5 to complete the sealing.

[0029] Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit. Although the present invention has been described in detail through the above preferred embodiments, those skilled in the art should understand that various changes can be made in form and details without departing from the scope defined by the claims of the present invention.

Claims

1. A wide-channel microfluidic chip sealing fixture, characterized in that: It includes an upper cover plate, a lower plate, side-end fasteners, a straight-through tube, horizontal fastening screws and vertical fastening screws; The upper cover plate is correspondingly arranged above the lower plate. A groove for placing a microfluidic chip is formed in the lower plate. The side-end fasteners are arranged in the groove of the lower plate to fasten the microfluidic chip from both sides. Matching screw holes and vertical fastening screws are formed in the upper cover plate and the lower plate in the vertical direction to connect and fasten the upper cover plate and the lower plate to the microfluidic chip. Screw holes and horizontal fastening screws are formed in the lower plate in the horizontal direction to squeeze the side-end fasteners from both sides to fasten the microfluidic chip. Through holes for communicating the sample inlets at both ends of the microfluidic chip are formed on both sides of the lower plate. The straight-through tube is arranged in the through holes to connect with the sample inlets of the microfluidic chip.

2. The sealing fixture for a wide-channel microfluidic chip according to claim 1, characterized in that: The height of the side-end fastener is 0.5-1 mm higher than the end face of the lower plate of the microfluidic chip fixture.

3. A wide-channel microfluidic chip sealing fixture according to claim 1, characterized in that: The number of the vertical fastening screws is 4, which are distributed on both sides of the microfluidic chip, 2 on each side.

4. A wide-channel microfluidic chip sealing fixture according to claim 1, characterized in that: The number of the horizontal fastening screws is 4, which are distributed on both sides of the lower plate, 2 on each side.

5. A wide-channel microfluidic chip sealing fixture according to claim 1, characterized in that: The material of the side-end fastener is polytetrafluoroethylene.

6. The sealing fixture for a wide-channel microfluidic chip according to claim 1, characterized in that: The fixture further includes a pressing head, a Luer connector and a Teflon hard tube. The pressing head is connected to one end of the straight-through tube. The Luer connector is connected to the pressing head. The Teflon hard tube is connected to the Luer connector.

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