Colloidal gold card colorimetric stand

Abstract

The utility model provides a colloidal gold card colorimetric stand. The colloidal gold card colorimetric stand comprises a bracket and a transparent colorimetric card, the bracket is composed of a bottom frame and an upper surface, the upper surface of the bracket is a transparent surface, a through hole is formed in the upper surface of the bracket, and a magnifying lens is embedded in the through hole; the transparent colorimetric card is provided with different gray scales, and the transparent colorimetric card is arranged on the upper surface of the bracket and can move, so that when the serum titer is measured by a colloidal gold method, a colloidal gold card detection line is arranged below the magnifying lens, and the transparent colorimetric card is observed and dragged through the magnifying lens; therefore, the gray scale of the detection line of the colloidal gold card is compared with different gray scale marks of the transparent colorimetric card. According to the utility model, a colloidal gold method determination result can be rapidly semi-quantified by utilizing an eye observation method, a power supply is not needed, complex software analysis is not needed, and the detection result can be rapidly and preliminarily quantified within 30 minutes.

Description

Technical Field

[0001] The utility model relates to the technical field of colloidal gold assay, and more specifically, to a colloidal gold card colorimetric rack. Background Art

[0002] The colloidal gold detection technology utilizes the principle of specific binding between antigen and antibody in immunology and is completed during the chromatography process through a chromogenic medium. It is widely used in the fields of food safety, medical testing, animal antibody monitoring, etc. Currently, this technology is mostly used for on-site detection and can obtain test results within 30 minutes. The determination method of the test results generally adopts the method of visual inspection or instrument detection. Visual inspection mainly depends on whether there is a line on the "test line" (T line) of the colloidal gold card. If there is, it is determined as positive, otherwise it is negative. Instrument detection is to scan the "test line" (T line) on the colloidal gold card through an instrument, measure the gray scale, and perform semi-quantification through the gray scale value to determine the test results.

[0003] However, the visual inspection method of colloidal gold can only quickly determine negative or positive, with strong subjectivity and it is difficult to have a relatively accurate judgment on the quantity. The instrument detection method can perform semi-quantitative determination, but it also has deficiencies: the instrument is relatively expensive and requires the assistance of power supply for use, which limits the use of the instrument in the interpretation of colloidal gold cards. Content of the Utility Model

[0004] In order to solve the deficiencies of the above-mentioned prior art, the purpose of the utility model is to provide a colloidal gold card colorimetric rack to overcome the defects in the prior art.

[0005] To achieve the above purpose, the utility model provides a colloidal gold card colorimetric rack, which includes a bracket and a transparent colorimetric card; wherein, the bracket is a bracket composed of a bottom frame and an upper surface, the upper surface of the bracket is a transparent surface, through holes are provided on the upper surface of the bracket, and magnifying lenses are inlaid in the through holes; different gray scale marks are provided on the transparent colorimetric card, and the transparent colorimetric card is placed on the upper surface of the bracket and can be moved, so that when the serum titer is determined by the colloidal gold method, the test line of the colloidal gold card is placed under the magnifying lens, and through the magnifying lens, the transparent colorimetric card is dragged to realize the comparison between the gray scale of the test line of the colloidal gold card and the different gray scale marks on the transparent colorimetric card.

[0006] Through the above technical solution, place the transparent color comparison card on the upper surface of the bracket, pass the colloidal gold test card through the bracket and place it at an appropriate position below the magnifying lens, making the test line on the colloidal gold test card face the magnifying lens. Move it up and down by hand to adjust the focal length so that the naked eye can clearly see the test line from above the magnifying lens, achieving the effect of observing the gray scale of the test line through the magnifying glass. Drag the transparent color comparison card, compare the gray scale of the test line on the colloidal gold test card with the different gray scale markings on the transparent color comparison card, and find the gray scale marking on the transparent color comparison card that is close to the gray scale of the test line, realizing the semi-quantification of the determination result of the colloidal gold method by the visual method and determining the range of the test result.

[0007] As a further description of the colloidal gold card color comparison stand of the present utility model, preferably, the through hole is located at the center of the upper surface and the aperture of the through hole is 3 cm.

[0008] Through the above technical solution, the aperture of the through hole is set to 3 cm, which is convenient for the magnifying lens embedded in the through hole to clearly observe the gray scale of the test line.

[0009] As a further description of the colloidal gold card color comparison stand of the present utility model, preferably, the upper surface of the bracket is a transparent surface made of polyethylene.

[0010] As a further description of the colloidal gold card color comparison stand of the present utility model, preferably, the material of the bottom frame of the bracket is wood.

[0011] As a further description of the colloidal gold card color comparison stand of the present utility model, preferably, the different gray scale markings on the transparent color comparison card are formed by inkjet spraying on the surface of the transparent color comparison card.

[0012] Through the above technical solution, it is convenient to spray the gray scale markings on the transparent color comparison card by inkjet, and it is convenient to clean so that the transparent color comparison card can be reused.

[0013] As a further description of the colloidal gold card color comparison stand of the present utility model, preferably, the transparent color comparison card is made of polyethylene.

[0014] As a further description of the colloidal gold card color comparison stand of the present utility model, preferably, the different gray scale markings on the transparent color comparison card are arranged in the order from light to dark.

[0015] Through the above technical solution, the gray scale markings are arranged in the order from light to dark on the transparent color comparison card, which is convenient to find the gray scale marking on the transparent color comparison card that is close to the gray scale of the test line.

[0016] The beneficial effects of the present utility model are as follows: The present utility model can use the visual method to complete the gray-scale comparison of the "test line" (T line) on different colloidal gold test strips through a colorimetric rack, quickly and accurately semi-quantify the determination result of the colloidal gold method and determine the range of the test result. The ELISA antibody titer that the present utility model can measure is between 1:8 and 1:64, and the coincidence rate with the detection result of the ELISA antibody detection kit is about 80%; it does not require a power supply and does not require complex software analysis, and can preliminarily quantify the test result within 30 minutes; it has wide applicability and can complete the preliminary quantification of harmful substances in food and antigen-antibody in medical detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1A is a schematic structural view of the bracket of the present utility model;

[0018] Figure 1B is a schematic structural view of the transparent colorimetric card of the present utility model;

[0019] Figure 2 is a schematic structural view of the transparent colorimetric card with gray-scale markings corresponding to 0, 1:8, 1:16, 1:32, and 1:64 titers in an embodiment of the present utility model.

[0020] In the figure: bracket 1, chassis 11, upper surface 12, through hole 13, magnifying lens 14, and transparent colorimetric card 2. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0021] In order to further understand the structure, characteristics, and other purposes of the present utility model, the following is a detailed description with reference to the attached preferred embodiments and accompanying drawings. The embodiments described by the drawings are only used to illustrate the technical solutions of the present utility model and do not limit the present utility model.

[0022] As Figure 1A and Figure 1B shown, the present utility model provides a colloidal gold card colorimetric rack, which is composed of a bracket 1 and a transparent colorimetric card 2.

[0023] Referring to Figure 1A , the bracket 1 is a bracket composed of a chassis 11 and an upper surface 12. The upper surface 12 of the bracket 1 is a transparent surface. Preferably, the upper surface 12 of the bracket 1 is a transparent surface made of polyethylene. The material of the chassis 11 of the bracket 1 is wood.

[0024] A through hole 13 is provided on the upper surface 12 of the bracket 1, and a magnifying lens 14 is embedded in the through hole 13. Preferably, the through hole 13 is located at the center of the upper surface 12 and the aperture of the through hole 13 is 3 cm, so that the magnifying lens 14 embedded in the through hole 13 can clearly observe the gray scale of the test line.

[0025] Referring toFigure 1B , different gray-scale markings are provided on the transparent color comparison card 2. Preferably, the different gray-scale markings on the transparent color comparison card 2 are formed by inkjet spraying on the surface of the transparent color comparison card 2. The inkjet method is convenient for spraying gray-scale markings on the transparent color comparison card and for cleaning, so that the transparent color comparison card can be reused. More preferably, the different gray-scale markings on the transparent color comparison card 2 are arranged in the order from light to dark, which is convenient for finding the gray-scale markings on the transparent color comparison card that are similar to the gray-scale of the test line.

[0026] The transparent color comparison card 2 is placed on the upper surface 12 of the bracket 1 and is movable. The bracket 1 and the transparent color comparison card 2 can be used in combination with a colloidal gold test card with a test line. When measuring the serum titer by the colloidal gold method, place the test line of the colloidal gold card under the magnifying lens 14, observe through the magnifying lens 14, drag the transparent color comparison card 2, and compare the gray-scale of the test line of the colloidal gold card with the different gray-scale markings on the transparent color comparison card 2. Find the antibody titer corresponding to the gray-scale on the transparent color comparison card 2 that is similar to the gray-scale of the test line of the colloidal gold card, which is the bovine viral diarrhea E2 antibody titer of the measured serum.

[0027] It is proved by experiments that the ELISA antibody titer that can be measured by the present utility model is between 1:8 and 1:64, and the coincidence rate with the detection result of the ELISA antibody detection kit is about 80%. And in this way, the bovine viral diarrhea virus E2 antibody titer can be determined within 30 minutes.

[0028] In an embodiment of the present utility model, taking the bovine viral diarrhea virus antibody colloidal gold E2 antibody test card as an example. The transparent color comparison card 2 is prepared from polyethylene. The ELISA method is used to measure the E2 antibody of bovine viral diarrhea virus positive serum, and it is gradient diluted to each antibody titer of 1:8, 1:16, 1:32, 1:64, etc. with PBS (phosphate buffered saline) (0.01 mol / L, pH value 7.8). The gradient-diluted serum is measured with a colloidal gold test card, and the gray-scale values of the test lines on each colloidal gold test card are recorded. The different gray-scales are sprayed on the surface of the transparent color comparison card 2 by inkjet to form Figure 2 the transparent color comparison card 2 shown in the figure. There are 5 gray-scale lines with different depths on the transparent color comparison card 2 in this embodiment, corresponding to the titers of 0, 1:8, 1:16, 1:32, and 1:64 respectively. The transparent color comparison card with the required markings can be made according to needs by the above method.

[0029] When using the colloidal gold method to measure the serum titer, first place the Figure 1B transparent color comparison card 2 in Figure 1AOn the upper surface 12 of the bracket 1 in it. Take the colloidal gold test strip for bovine viral diarrhea virus E2 antibody, and operate according to the "Instruction Manual" of the colloidal gold test strip. Pass the colloidal gold test strip with both the test line (T line) and the control line (C line) appearing through the bracket 1 and place it at a suitable position under the magnifying lens 14. Make the "test line" (T line) on the colloidal gold test strip face the magnifying lens 14, and move it up and down by hand to adjust the focal length so that the naked eye can clearly see the "test line" (T line) from above the magnifying lens 14. Through the function of the magnifying glass, observe the gray scale of the "test line" (T line). Since the transparent color comparison card 2 is placed on the upper surface 12 of the bracket 1 and can be moved, at this time, drag the transparent color comparison card 2 placed on the upper surface 12 of the bracket 1, and compare the gray scale of the "test line" (T line) with the gray scale marks of each gradient on the transparent color comparison card 2 to find the gray scale of the "test line" (T line) that is close to the gray scale on the transparent color comparison card 2. The corresponding antibody titer is the titer of the bovine viral diarrhea E2 antibody in the measured serum.

[0030] It is proved by experiments that the ELISA antibody titer that can be measured by the present utility model is between 1:8 and 1:64, and the coincidence rate with the detection result of the ELISA antibody detection kit is about 80%. And in this way, the titer of bovine viral diarrhea virus E2 antibody can be determined within 30 minutes.

[0031] Therefore, the upper colloidal gold card color comparison rack can complete the comparison of the gray scales of different "test lines" (T lines) through the color comparison rack by the visual method, can quickly semi-quantify the determination result of the colloidal gold method and determine the range of the detection result; it does not require a power supply and does not need complex software analysis, and can quickly perform a preliminary quantification on the detection result; it has wide applicability and can complete the preliminary quantification of harmful substances in food and antigen-antibody in medical detection.

[0032] It should be stated that the above-mentioned utility model content and specific implementation manners are intended to prove the practical application of the technical solution provided by the present utility model, and should not be construed as a limitation of the protection scope of the present utility model. Those skilled in the art can make various modifications, equivalent replacements or improvements within the spirit and principle of the present utility model. The protection scope of the present utility model shall be subject to the appended claims.

Claims

1. A colloidal gold colorimetric rack, characterized in that, The colloidal gold colorimetric stand includes a bracket (1) and a transparent colorimetric card (2); among them, the bracket (1) is a bracket composed of a bottom frame (11) and an upper surface (12). The upper surface (12) of the bracket (1) is a transparent surface. A through hole (13) is provided on the upper surface (12) of the bracket (1), and a magnifying lens (14) is embedded in the through hole (13); the transparent colorimetric card (2) is provided with different gray-scale markings. The transparent colorimetric card (2) is placed on the upper surface (12) of the bracket (1) and can be moved. When the serum titer is measured by the colloidal gold method, the detection line of the colloidal gold card is placed below the magnifying lens (14), and the transparent colorimetric card (2) is dragged to observe through the magnifying lens (14), so as to compare the gray scale of the detection line of the colloidal gold card with the different gray-scale markings of the transparent colorimetric card (2).

2. The colloidal gold colorimetric rack according to claim 1, characterized in that, The through hole (13) is located at the center of the upper surface (12) and the aperture of the through hole (13) is 3 cm.

3. The colloidal gold colorimetric rack according to claim 1, wherein, The upper surface (12) of the bracket (1) is a transparent surface made of polyethylene.

4. The colloidal gold colorimetric rack according to claim 1, characterized in that, The material of the bottom frame (11) of the bracket (1) is wood.

5. The colloidal gold colorimetric rack according to claim 1, characterized in that, The different gray-scale markings of the transparent colorimetric card (2) are formed by inkjet spraying on the surface of the transparent colorimetric card (2).

6. The colloidal gold colorimetric rack according to claim 1, characterized in that, The transparent colorimetric card (2) is made of polyethylene.

7. The colloidal gold colorimetric rack according to claim 1, characterized in that The different gray-scale markings on the transparent colorimetric card (2) are arranged in the order from light to dark.

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