Cryopreservation tube

Abstract

The utility model discloses a cryopreservation tube which comprises a tube body used for storing biological samples, a first tube cap, a collecting tube, a balance tube and an air filter. The first pipe cap is detachably connected with one end of the pipe body; the collecting pipe and the balance pipe are arranged on the first pipe cap, and both the collecting pipe and the balance pipe are communicated with the pipe body; the air filter is arranged on the balance pipe and is used for filtering gas entering the pipe body; when a biological sample is subpackaged, the collecting pipe is communicated with a high-capacity biological sample container, the biological sample flows into the pipe body through the collecting pipe, and the balance pipe and the air filter are arranged to balance air pressure inside and outside the pipe body and ensure that the biological sample can smoothly flow into the pipe body; when the cryopreservation tube is used for subpackaging biological samples, convenience and rapidness are achieved, and subpackaging operation is simple and efficient.

Description

Technical Field

[0001] The utility model belongs to the technical field of cryopreservation for laboratory testing, and particularly relates to a cryopreservation tube. Background Art

[0002] Cryopreservation tubes are mainly used for low-temperature transportation and storage of tissue or cell samples, and are commonly used in the fields of biological research and medicine. The traditional design of cryopreservation tubes is usually that the upper cap and the tube body are sealed for cryopreservation. The cell sample is transferred into the tube body through a suction device in a sterile environment, and then sealed with the upper cap and placed in the storage environment. This has the limitation that it cannot be directly sub-packed from a large-capacity biological sample container into a small-capacity cell sample container. Content of the Utility Model

[0003] In order to overcome the deficiencies of the prior art, the utility model provides a cryopreservation tube that is convenient for sub-packaging.

[0004] The technical solution adopted by the utility model to solve its technical problems is as follows:

[0005] A cryopreservation tube includes a tube body for storing biological samples, a first tube cap, a collection tube, a balance tube, and an air filter;

[0006] The first tube cap is detachably connected to one end of the tube body;

[0007] The collection tube and the balance tube are arranged on the first tube cap, and both the collection tube and the balance tube are communicated with the tube body;

[0008] The collection tube is used to communicate with a large-capacity biological sample container;

[0009] The air filter is arranged on the balance tube to filter the gas entering the tube body.

[0010] For the cryopreservation tube as described above, a first sealing ring is arranged on the first tube cap, and the first sealing ring is used to fill the gap between the first tube cap and the tube body.

[0011] For the cryopreservation tube as described above, when loading the biological sample into the tube body, the collection tube is in a state of being communicated with the outside environment, and the biological sample can be loaded into the tube body through the collection tube;

[0012] After loading the biological sample into the tube body, the collection tube is in a sealed state to isolate the tube body from the outside environment.

[0013] For the cryopreservation tube as described above, it further includes a sealing film and a puncturing member, and the sealing film is arranged at one end of the tube body away from the first tube cap;

[0014] An outer peripheral component is connected to the piercing member, and the piercing member is used to pierce the sealing film to communicate the tube body with the outer peripheral component.

[0015] For the cryopreservation tube as described above, a through groove is provided on the piercing member; when the piercing member pierces the sealing film, the through groove communicates the tube body with the outer peripheral component.

[0016] For the cryopreservation tube as described above, the piercing member includes a spike portion and a second tube cap. The spike portion is used to pierce the sealing film, and the through groove communicates the spike portion and the second tube cap.

[0017] For the cryopreservation tube as described above, a sterile absorbent foam is provided in the balance tube.

[0018] For the cryopreservation tube as described above, a second sealing ring is provided on the second tube cap. When the piercing member pierces the sealing film, the second sealing ring is used to fill the gap between the second tube cap and the tube body.

[0019] For the cryopreservation tube as described above, a pneumatic balance member is further provided on the piercing member. When the piercing member pierces the sealing film, the pneumatic balance member is used to balance the air pressure inside and outside the tube body.

[0020] For the cryopreservation tube as described above, the sealing film includes a low-temperature resistant film provided inside the tube body and a protective film provided outside the tube body.

[0021] The beneficial effects of the present utility model are as follows:

[0022] On the first tube cap detachably connected to the tube body, a collection tube and a balance tube communicating with the tube body are provided. When aliquoting biological samples, the collection tube is connected to a large-capacity biological sample container, and the biological samples flow into the tube body through the collection tube. The balance tube and the air filter are provided to balance the air pressure inside and outside the tube body, ensuring that the biological samples can flow smoothly into the tube body; using the cryopreservation tube of the present utility model to aliquot biological samples is convenient and fast, and the aliquoting operation is simple and efficient. Description of the Drawings

[0023] The present utility model will be further described below with reference to the drawings and embodiments.

[0024] Figure 1 is one of the structural schematic diagrams of the cryopreservation tube in the present utility model (section view);

[0025] Figure 2 is the second structural schematic diagram of the cryopreservation tube in the present utility model (section view);

[0026] Figure 3 is Figure 2 the enlarged structural view of part A in

[0027] The reference numerals are as follows:

[0028] 1 - Tube body; 2 - First tube cap; 21 - First sealing ring; 3 - Sealing film; 31 - Low - temperature resistant film; 32 - Protective film; 4 - Piercing member; 41 - Spiked part; 411 - Through groove; 42 - Second tube cap; 43 - Second sealing ring; 51 - Collection tube; 511 - First - stage collection tube; 512 - Second - stage collection tube; 52 - Balance tube; 53 - Air filter. Detailed implementation manners

[0029] The following will clearly and completely describe the concept, specific structure and technical effects generated by the present utility model in combination with the embodiments and the drawings, so as to fully understand the purpose, features and effects of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all embodiments. Based on the embodiments of the present utility model, other embodiments obtained by those skilled in the art without creative efforts all fall within the scope of protection of the present utility model. In addition, all the connection / linkage relationships involved in the patent do not simply refer to the direct connection of components, but refer to the more optimal connection structure that can be formed by adding or reducing connection accessories according to the specific implementation situation. Each technical feature in the present creation of the utility model can be interactively combined without mutual contradiction and conflict.

[0030] Both the cryopreservation tube and the cryopreservation bag are containers for storing biological samples. After injecting the biological sample into them, they are placed in a low - temperature environment to reduce the activity of the biological sample; when the biological sample is needed, the cryopreservation tube or the cryopreservation bag is taken out from the low - temperature environment and placed in a warm environment to thaw the biological sample and activate the activity of the biological sample.

[0031] Refer to Figure 1 、 Figure 2 In this embodiment, a cryopreservation tube is provided, which includes a tube body 1 for storing biological samples, a first tube cap 2, a collection tube 51, a balance tube 52 and an air filter 53.

[0032] The first tube cap 2 is detachably connected to one end of the tube body 1.

[0033] The collection tube 51 and the balance tube 52 are arranged on the first tube cap 2, and both the collection tube 51 and the balance tube 52 are communicated with the tube body 1.

[0034] The collection tube 51 is used to communicate with a large - capacity biological sample container.

[0035] The air filter 53 is arranged on the balance tube 52 to filter the gas entering the tube body 1.

[0036] When aliquoting biological samples, the collection tube 51 can be connected to a large-capacity biological sample container through methods such as heat-sealing and connecting the pipeline with a sterile pipe connection device, and the biological sample flows into the tube body 1 through the collection tube 51; a balance tube 52 and an air filter 53 are provided to balance the air pressure inside and outside the tube body 1. When injecting the biological sample into the tube body 1 along the collection tube 51, the air pressure inside the tube body 1 is not balanced with the air pressure of the external environment, making it difficult to inject the biological sample into the tube body 1. Therefore, an air filter 53 is provided, which can balance the air pressure inside and outside the tube body 1, facilitating the filling of biological samples. And to prevent pollutants in the external environment from entering during the filling process, the air filter 53 can remove particles, bacteria, and viruses in the air, keeping the biological sample inside the tube body 1 clean and ensuring that the biological sample can flow smoothly into the tube body; Using the cryotube of the present utility model to aliquot biological samples is convenient and fast, and the aliquoting operation is simple and efficient;

[0037] After aliquoting, a heat sealer can be used to heat-seal and close the collection tube 51 to prevent the biological sample from flowing out of the collection tube 51; after heat-sealing, the first tube cap 2 faces upward, and the cryotube is placed into a cryogenic storage device;

[0038] Moreover, when injecting the biological sample into the tube body 1, if the first tube cap 2 is opened, the contact area between the inside of the tube body 1 and the external environment will increase, which may cause pollutants in the external environment to enter the tube body 1. Injecting the biological sample into the tube body 1 along the collection tube 51 does not require opening the first tube cap 2, and since the radius of the collection tube 51 is small, the contact area between the collection tube 51 and the external environment is also small. Injecting the biological sample into the tube body 1 along the collection tube 51 can reduce the chance of contact between the inside of the tube body 1 and the external environment and reduce the probability of pollutants in the external environment entering the tube body 1.

[0039] As an explanation, the biological sample can be cell fluid, tissue fluid, blood, DNA, RNA, etc.

[0040] The cryotube in this embodiment has the advantages of greater hardness, better support, and more convenient placement compared to a cryobag:

[0041] The tube wall of the cryotube generally has a certain hardness, while the cryobag is flexible. Therefore, the hardness of the cryotube is greater than that of the cryobag. In a low-temperature environment of -196°C, if the cryobag lacks support or is not properly handled during the removal process, the cryobag will deform and break during cryopreservation, resulting in the outflow of cell fluid, which will not only contaminate the cryobox but also cause the biological sample to become invalid; while the cryotube has much better low-temperature resistance performance and will not break even in a low-temperature environment of -196°C;

[0042] When storing cryopreservation bags, since the outer wall of the cryopreservation bag is very soft, the cryopreservation bag needs to be placed in a support plate for support, and then the cryopreservation bag is placed in a cryobox, which is inconvenient for cryopreservation; while the wall thickness of the cryotube is greater than that of the cryopreservation bag, and its tube wall itself has a supporting effect, so when storing cryotubes, there is no need to set up an additional supporting structure; therefore, in the same cryobox, more cryotubes can be placed, and more biological samples can be stored in the same cryobox, making full use of the storage space of the cryobox.

[0043] Among them, the manufacturing materials of the tube body 1, the first tube cap 2 and the sealing film 3 can be made of low-temperature resistant materials. For example, the tube body 1 and the first tube cap 2 are made of polypropylene materials, and the sealing film 3 is made of metal or EVA (ethylene-vinyl acetate copolymer) materials; since the piercing member 4 is only needed after the cryotube is thawed (or after the biological sample recovery operation), the piercing member 4 does not need to be made of low-temperature resistant materials.

[0044] Among them, the first tube cap 2 is threadedly connected to the tube body 1.

[0045] Specifically, a first sealing ring 21 is provided between the first tube cap 2 and the tube body 1, and the first sealing ring 21 is used to fill the gap between the first tube cap 2 and the tube body 1, isolate the inside of the tube body 1 from the external environment, and seal the tube body 1.

[0046] In one embodiment, it further includes a sealing film 3 and a piercing member 4, and the sealing film 3 is arranged at one end of the tube body 1 away from the first tube cap 2;

[0047] The piercing member 4 is connected with a peripheral component, and the piercing member 4 is used to pierce the sealing film 3 to communicate the tube body 1 with the peripheral component.

[0048] When an operator wants to take out the biological sample in the tube body 1, if the cryotube has not completely returned to room temperature, the tube body 1 and the first tube cap 2 are in a tightly combined state, and it is difficult to unscrew the first tube cap 2 from the tube body 1; at this time, the piercing member 4 can be used to pierce the sealing film 3 for sampling; of course, when the cryotube returns to room temperature, sampling can still be carried out by piercing the sealing film 3 with the piercing member 4; the cryotube in this embodiment can take liquid in both directions, enriching the liquid-taking form of the cryotube and providing more sampling options for the operator.

[0049] In one embodiment, when loading the biological sample into the tube body 1, the collection tube 51 is in a communicating state with the large-capacity biological sample container, and the biological sample (such as cell sample) in the large-capacity biological sample container can be loaded into the tube body 1 through the collection tube 51;

[0050] After loading the biological sample into the tube body 1, a heat sealer is used to heat seal the collection tube 51, making the collection tube 51 in a sealed state. While cutting off the connection between the tube body 1 and the large-capacity biological sample container, the connection between the tube body 1 and the external environment is isolated.

[0051] Specifically, the connection parts of the collection tube 51 and the large-capacity biological sample container are both made of polymer materials. Before the collection tube 51 is connected to the large-capacity biological sample container, the collection tube 51 and the large-capacity biological sample container can be melt-connected through a sterile connecting device.

[0052] Specifically, the collection tube 51 includes a first-stage collection tube 511 and a second-stage collection tube 512 that are connected to each other; the second-stage collection tube 512 is vertically connected to the first tube cap 2 and the first-stage collection tube 511 is inclined with respect to the second-stage collection tube 512; this can further reduce the probability of contaminants entering the inside of the tube body 1 under the action of gravity and reduce the occurrence of contamination of the biological sample in the tube body 1.

[0053] Before freezing the cryotube, it needs to be sealed. More specifically, both the collection tube 51 and the balance tube 52 are made of heat-meltable materials. After filling the biological sample, a heat-sealing device is used to heat-seal the collection tube 51 and / or the balance tube 52.

[0054] In one embodiment, a through groove 411 is provided on the piercing member 4; when the piercing member 4 pierces the sealing film 3, the through groove 411 connects the tube body 1 and the peripheral components. The through groove 411 serves as a channel for the biological sample to flow out of the tube body 1. After the piercing member 4 pierces the sealing film 3, it does not need to be removed from the tube body 1 and can also allow the biological sample in the tube body 1 to flow out.

[0055] As an explanation, the peripheral components can be a catheter, a sealing nut, a sampling syringe, etc. After the biological sample in the tube body 1 flows through the through groove 411 to the peripheral components, it flows into a specific container for later use.

[0056] Specifically, the piercing member 4 includes a spike part 41 and a second tube cap 42. The spike part 41 is used to pierce the sealing film 3, and the through groove 411 is arranged to connect the spike part 41 and the second tube cap 42. Among them, the function of the second tube cap 42 is to fix the relative position of the spike part 41 and the tube body 1. For example, after the spike part 41 pierces the sealing film 3, the peripheral components can be connected to the through groove 411 to lead the biological sample in the tube body 1 to a specific container.

[0057] More specifically, the tube body 1 is threadedly connected to the second tube cap 42, which can precisely control the depth of the spike part 41 entering the tube body 1 and can firmly fix the piercing member 4 on the tube body 1, facilitating the insertion of a catheter or other operations on the piercing member 4.

[0058] More specifically, a second sealing ring 43 is provided on the second tube cap 42. After the piercing member 4 pierces the sealing film 3, the second sealing ring 43 is used to fill the gap between the second tube cap 42 and the tube body 1. On the one hand, it can prevent the biological sample from flowing out along the gap between the second tube cap 42 and the tube body 1, reducing the waste of the biological sample. On the other hand, it can also prevent pollutants in the external environment from entering the housing 1 and contaminating the biological sample to be used.

[0059] In one embodiment, a sterile absorbent foam is provided in the balance tube 52. Before taking out the biological sample from the cryopreservation tube, the cryopreservation tube needs to be taken out of the cryopreservation equipment to thaw and revive the cells. If the balance tube 52 on the cryopreservation tube is not heat-sealed and the air filter 53 is retained, air will condense into water vapor during the thawing process and enter the balance tube 52 through the air filter 53, contaminating the biological sample in the tube body 1. By providing a sterile absorbent foam in the balance tube 52, the water vapor can be absorbed, preventing the biological sample from being contaminated. Moreover, the sterile absorbent foam has a porous structure and will not block the balance tube 52.

[0060] In any of the above embodiments, the sealing film 3 includes a low-temperature resistant film 31 provided inside the tube body 1 and a protective film 32 provided outside the tube body 1, and there is a distance between the low-temperature resistant film 31 and the protective film 32. The low-temperature resistant film 31 can maintain its structural strength even in a low-temperature environment, preventing it from breaking due to environmental temperature changes and causing the leakage of the biological sample. The protective film 31 can be used to protect the low-temperature resistant film 31 and prevent the low-temperature resistant film from being damaged during the operation of injecting the biological sample or freezing and storing.

[0061] As an example, the low-temperature resistant film 31 is made of EVA material, and the protective film 32 is made of aluminum foil.

[0062] In one embodiment, the piercing member 4 further includes a pressure balance member (not shown in the figure). After the piercing member 4 pierces the sealing film 3, the pressure balance member can be used to balance the air pressure inside and outside the tube body 1.

[0063] When the biological sample flows out along the through groove 411, the air pressure inside and outside the tube body 1 is inconsistent, which will cause the biological sample to be difficult to flow out. Setting the pressure balance member can balance the air pressure inside and outside the tube body 1, facilitating the taking out of the biological sample.

[0064] The above is a specific description of the preferred embodiments of the present invention, but the present invention is not limited to the above embodiments. Those skilled in the art can make various equivalent deformations or substitutions without departing from the spirit of the present invention, and these equivalent deformations or substitutions are all included in the scope defined by the claims of this application.

Claims

1. A cryotube, characterized in that: It includes a tube body (1) for storing biological samples, a first tube cap (2), a collection tube (51), a balance tube (52), and an air filter (53); The first tube cap (2) is detachably connected to one end of the tube body (1); The collection tube (51) and the balance tube (52) are arranged on the first tube cap (2), and both the collection tube (51) and the balance tube (52) are communicated with the tube body (1); The collection tube (51) is used to communicate with a large-capacity biological sample container; The air filter (53) is arranged on the balance tube (52) to filter the gas entering the tube body (1).

2. The cryotube according to claim 1, wherein: A first sealing ring (21) is arranged on the first tube cap (2), and the first sealing ring (21) is used to fill the gap between the first tube cap (2) and the tube body (1).

3. The cryogenic vial according to claim 1, wherein: When loading biological samples into the tube body (1), the collection tube (51) is in a state of being communicated with the large-capacity biological sample container, and the biological samples in the large-capacity biological sample container can be loaded into the tube body (1) through the collection tube (51); After loading the biological samples into the tube body (1), the collection tube (51) is in a sealed state to isolate the tube body (1) from the large-capacity biological sample container.

4. The cryotube according to claim 1, characterized in that: It further includes a sealing film (3) and a puncturing member (4), and the sealing film (3) is arranged at one end of the tube body (1) away from the first tube cap (2); An external component is connected to the puncturing member (4), and the puncturing member (4) is used to puncture the sealing film (3) to communicate the tube body (1) with the external component.

5. The cryogenic vial according to claim 4, characterized in that: A through groove (411) is arranged on the puncturing member (4); when the puncturing member (4) punctures the sealing film (3), the through groove (411) communicates the tube body (1) with the external component.

6. The cryogenic tube according to claim 5, wherein: The puncturing member (4) includes a spike portion (41) and a second tube cap (42), the spike portion (41) is used to puncture the sealing film (3), and the through groove (411) is arranged to communicate the spike portion (41) and the second tube cap (42).

7. The cryotube according to claim 1, wherein: A sterile absorbent foam is arranged in the balance tube (52).

8. The cryotube according to claim 6, characterized in that: A second sealing ring (43) is arranged on the second tube cap (42), and after the puncturing member (4) punctures the sealing film (3), the second sealing ring (43) is used to fill the gap between the second tube cap (42) and the tube body (1).

9. The cryogenic storage tube according to claim 4, wherein: An air pressure balancing member is further arranged on the puncturing member (4), and after the puncturing member (4) punctures the sealing film (3), the air pressure balancing member is used to balance the air pressure inside and outside the tube body (1).

10. The cryogenic tube according to any one of claims 4-6, 8, and 9, characterized in that: The sealing film (3) includes a low-temperature resistant film (31) arranged inside the tube body (1) and a protective film (32) arranged outside the tube body (1).

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