Biomacromolecule separation system

Abstract

The utility model relates to the technical field of biomacromolecule separation, in particular to a biomacromolecule separation system, which adopts the technical scheme that the biomacromolecule separation system comprises a separation box, a cover plate, a first mounting plate and a threaded rod, a glass observation window is arranged at the front end of the separation box, and mounting rods which are symmetrically distributed are mounted on the inner wall of the separation box; the cover plate covers the upper end of the separation box, a rotating shaft is rotatably mounted at the lower end of the cover plate, an inner cover is mounted at the tail end of the rotating shaft, a lower mounting plate and an upper mounting plate are mounted on the inner wall of the separation box, the threaded rod is rotatably mounted between the lower mounting plate and the upper mounting plate, and a lifting block is mounted on the threaded rod in a threaded manner; an electric push rod is installed at the front end of the lifting block, and a material taking pipe is installed at the tail end of a piston rod of the electric push rod. The device disclosed by the utility model has the advantages that supernatant liquid and lower sediment are taken out in a suction manner, and biomacromolecules are prevented from being mixed again and waste of the biomacromolecules is prevented.

Description

Technical Field

[0001] The utility model relates to the technical field of biological macromolecule separation, in particular to a biological macromolecule separation system. Background Technique

[0002] Biological macromolecule separation is to separate biological macromolecules (such as proteins, nucleic acids, polysaccharides, etc.) from complex mixtures. The main methods of biological macromolecule separation include precipitation, ultrafiltration, dialysis, and centrifugation, etc. The centrifugation method is a method of separating biological macromolecules from a solution by using centrifugal force. According to different centrifugal forces and rotation speeds, biological macromolecules of different sizes and densities can be separated. The centrifugation method has the advantages of high separation efficiency and wide application range.

[0003] The biological macromolecule separation device separates biological macromolecules by centrifugal force. The biological macromolecules will precipitate at the bottom of the centrifuge tube. The staff needs to take out the centrifuge tube. When taking it out, it is easy to mix the separated biological macromolecules again. And after taking it out, it is necessary to carefully pour out the supernatant. When pouring, it is easy to cause the precipitate to be poured out, resulting in waste of biological macromolecules. Content of the Utility Model

[0004] The purpose of the utility model is to provide a biological macromolecule separation system, which has the advantages of taking out the supernatant and the lower precipitate by suction, preventing the biological macromolecules from being mixed again and preventing waste of biological macromolecules, and solves the problems raised in the above background technique.

[0005] To achieve the above purpose, the utility model provides the following technical scheme: A biological macromolecule separation system, including a separation box, a cover plate, a first mounting plate and a threaded rod. A glass observation window is arranged at the front end of the separation box, and symmetrically distributed mounting rods are installed on the inner wall of the separation box. The cover plate covers the upper end of the separation box. A rotating shaft is rotatably installed at the lower end of the cover plate, and an inner cover is installed at the end of the rotating shaft. A lower mounting plate and an upper mounting plate are installed on the inner wall of the separation box. The threaded rod is rotatably installed between the lower mounting plate and the upper mounting plate. A lifting block is threadedly installed on the threaded rod. An electric push rod is installed at the front end of the lifting block, and a material taking pipe is installed at the end of the piston rod of the electric push rod.

[0006] When using a biological macromolecule separation system of the present utility model, the material is added into a transparent centrifuge tube, and then the cover plate is installed. The inner cover will cover the transparent centrifuge tube. By the operation of the first driving device, the support plate can be rotated, so that the transparent centrifuge tube can be rotated to generate centrifugal force. Under the action of the centrifugal force, biological macromolecules can be separated. After the separation is completed, the cover plate is removed. By the operation of the electric push rod, the piston rod can be extended, so that the material taking tube can extend above the transparent centrifuge tube. By the operation of the second driving device, the threaded rod can be rotated. With the cooperation of the lifting block, the material taking tube can be lowered into the transparent centrifuge tube, so that the end of the material taking tube is located at the upper clear liquid. By the operation of the discharge pump, the upper clear liquid in the transparent centrifuge tube can be pumped out. Then the material taking tube is lowered again, so that the end of the material taking tube is located at the bottom of the transparent centrifuge tube, and the separated macromolecular precipitate can be pumped out. Different containers are used for the two material pumping operations.

[0007] Preferably, support legs are installed at the lower end of the separation box. There are four support legs in total, and the four support legs are arranged in an array with respect to the separation box.

[0008] Preferably, the first mounting plate is installed between the mounting rods. The upper end of the first mounting plate is rotatably installed with a support plate, and a positioning groove is provided on the support plate. The transparent centrifuge tube is arranged in the positioning groove.

[0009] Preferably, a first driving device is installed at the lower end of the first mounting plate, and the main shaft of the first driving device is connected to the axis of the support plate.

[0010] Preferably, symmetrically distributed first fixing plates are installed at the side end of the support plate. The second fixing plate is installed at the end of the first fixing plate. A spring is installed at the side end of the second fixing plate, a damper is arranged in the spring, and a positioning plate is installed at the end of the spring.

[0011] Preferably, a clamping block is installed at the upper part of the outer surface of the separation box, and a clamping buckle is installed at the side end of the cover plate. The clamping buckle is clamped on the clamping block.

[0012] Preferably, a second driving device is installed at the lower end of the lower mounting plate, and the main shaft of the second driving device is connected to the axis of the threaded rod.

[0013] Preferably, a limiting sliding groove is opened on the inner wall of the separation box, and a limiting sliding block is installed at the rear end of the lifting block. The limiting sliding block is slidably installed in the limiting sliding groove.

[0014] Preferably, a discharge pump is arranged at the rear end of the separation box. A suction pipe and a discharge pipe are arranged on the discharge pump, and the end of the suction pipe is connected to the material taking tube.

[0015] Compared with the prior art, the beneficial effects of the present utility model are as follows: By means of suction, the supernatant and the sediment at the lower layer are separately taken out without taking out the centrifuge tube, which can prevent the re - mixing of biological macromolecules. Moreover, by using the extraction method in batches, it can prevent the macromolecular sediment from being mixed in the supernatant and prevent the waste of biological macromolecules. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 is a front - view structural schematic diagram of the present utility model;

[0017] Figure 2 is a sectional - view structural schematic diagram of the present utility model;

[0018] Figure 3 is a rear - view structural schematic diagram of the present utility model;

[0019] Figure 4 is a partial bottom - view structural schematic diagram of the present utility model;

[0020] Figure 5 is a partial front - view structural schematic diagram of the present utility model;

[0021] Figure 6 is a bottom - view structural schematic diagram of the cover plate of the present utility model.

[0022] The reference numerals and names in the drawings are as follows:

[0023] 101, separation box; 102, support leg; 103, glass observation window; 104, cover plate; 105, clamping block; 106, buckle; 107, rotating shaft; 108, inner cover; 201, mounting rod; 202, first mounting plate; 203, first driving device; 204, support plate; 205, first fixing plate; 206, second fixing plate; 207, spring; 208, positioning plate; 209, positioning groove; 210, transparent centrifuge tube; 301, lower mounting plate; 302, upper mounting plate; 303, threaded rod; 304, second driving device; 305, lifting block; 306, electric push rod; 307, material - taking pipe; 401, discharge pump; 402, suction pipe; 403, discharge pipe. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0024] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0025] Embodiment 1

[0026] Please refer to Figures 1 to 6 , an embodiment provided by the present utility model: a biological macromolecule separation system, comprising:

[0027] A separation box 101, a cover plate 104, a first mounting plate 202 and a threaded rod 303. A glass observation window 103 is provided at the front end of the separation box 101. Symmetrically distributed mounting rods 201 are installed on the inner wall of the separation box 101. The cover plate 104 is covered on the upper end of the separation box 101. A rotating shaft 107 is rotatably installed at the lower end of the cover plate 104, and an inner cover 108 is installed at the end of the rotating shaft 107. A lower mounting plate 301 and an upper mounting plate 302 are installed on the inner wall of the separation box 101. The threaded rod 303 is rotatably installed between the lower mounting plate 301 and the upper mounting plate 302. A lifting block 305 is threadedly installed on the threaded rod 303. An electric push rod 306 is installed at the front end of the lifting block 305, and a material taking pipe 307 is installed at the end of the piston rod of the electric push rod 306.

[0028] In this embodiment, the material is added into the transparent centrifuge tube 210, and then the cover plate 104 is installed. The inner cover 108 will cover the transparent centrifuge tube 210. By the operation of the first driving device 203, the support plate 204 can be rotated, so that the transparent centrifuge tube 210 can rotate to generate centrifugal force. Under the action of the centrifugal force, the biological macromolecules can be separated. After the separation is completed, the cover plate 104 is removed. By the operation of the electric push rod 306, the piston rod can be extended, so that the material taking pipe 307 can extend above the transparent centrifuge tube 210. By the operation of the second driving device 304, the threaded rod 303 can be rotated. With the cooperation of the lifting block 305, the material taking pipe 307 can be lowered into the transparent centrifuge tube 210, so that the end of the material taking pipe 307 is located at the upper layer of the supernatant. By the operation of the discharge pump 401, the upper layer of the supernatant in the transparent centrifuge tube 210 can be pumped out. Then the material taking pipe 307 is lowered again, so that the end of the material taking pipe 307 is located at the bottom of the transparent centrifuge tube 210, and the separated macromolecular precipitate can be pumped out. Different containers are used for the two times of material extraction. [[ID=X]]

[0029] Furthermore, support legs 102 are installed at the lower end of the separation box 101. There are four support legs 102 in total, and the four support legs 102 are arranged in an array with respect to the separation box 1 / 01.

[0030] Furthermore, the first mounting plate 202 is installed between the mounting rods 201. A support plate 204 is rotatably installed at the upper end of the first mounting plate 202. A positioning groove 209 is provided on the support plate 204, and a transparent centrifuge tube 210 is provided in the positioning groove 209.

[0031] Furthermore, a first driving device 203 is installed at the lower end of the first mounting plate 202, and the main shaft of the first driving device 203 is connected to the axis of the support plate 204.

[0032] Furthermore, symmetrically distributed first fixing plates 205 are installed at the side ends of the support plate 204. A second fixing plate 206 is installed at the end of the first fixing plate 205. A spring 207 is installed at the side end of the second fixing plate 206. A damper is arranged inside the spring 207. A positioning plate 208 is installed at the end of the spring 207.

[0033] Furthermore, a clamping block 105 is installed at the upper position on the outer surface of the separation box 101. A clamping buckle 106 is installed at the side end of the cover plate 104. The clamping buckle 106 is clamped on the clamping block 105.

[0034] Furthermore, a second driving device 304 is installed at the lower end of the lower mounting plate 301. The main shaft of the second driving device 304 is connected to the axis of the threaded rod 303.

[0035] Furthermore, a limiting sliding groove is formed in the inner wall of the separation box 101. A limiting sliding block is installed at the rear end of the lifting block 305. The limiting sliding block is slidably installed in the limiting sliding groove.

[0036] Furthermore, a discharging pump 401 is arranged at the rear end of the separation box 101. A suction pipe 402 and a discharging pipe 403 are arranged on the discharging pump 401. The end of the suction pipe 402 is connected to the material taking pipe 307.

[0037] The devices in the present utility model are well-known devices. Their working principles and circuit connections are well-known to technicians in this field and all belong to conventional means or common general knowledge. The present utility model only utilizes their functions and does not improve their structures, so details are not described herein again. Those skilled in the art can make arbitrary selections according to their needs or convenience.

[0038] For those skilled in the art, it is obvious that the present utility model is not limited to the details of the above exemplary embodiments, and can be implemented in other specific forms without departing from the spirit or basic characteristics of the present utility model. Therefore, from any perspective, the embodiments should be regarded as exemplary and non-limiting. The scope of the present utility model is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present utility model. Any reference signs in the claims should not be regarded as limiting the claimed rights.

Claims

1. A biological macromolecule separation system, comprising a separation box (101), a cover plate (104), a first mounting plate (202), and a threaded rod (303), characterized in that: A glass observation window (103) is provided at the front end of the separation box (101). Mounting rods (201) are symmetrically distributed and installed on the inner wall of the separation box (101). The cover plate (104) covers the upper end of the separation box (101). A rotating shaft (107) is rotatably installed at the lower end of the cover plate (104), and an inner cover (108) is installed at the end of the rotating shaft (107). A lower mounting plate (301) and an upper mounting plate (302) are installed on the inner wall of the separation box (101). The threaded rod (303) is rotatably installed between the lower mounting plate (301) and the upper mounting plate (302). A lifting block (305) is threadedly installed on the threaded rod (303). An electric push rod (306) is installed at the front end of the lifting block (305), and a material taking pipe (307) is installed at the end of the piston rod of the electric push rod (306).

2. The biomacromolecule separation system according to claim 1, wherein: Support legs (102) are installed at the lower end of the separation box (101). There are four support legs (102) in total, and the four support legs (102) are arranged in an array with respect to the separation box (101).

3. The biomacromolecule separation system according to claim 1, wherein: The first mounting plate (202) is installed between the mounting rods (201). A support plate (204) is rotatably installed at the upper end of the first mounting plate (202). A positioning groove (209) is provided on the support plate (204), and a transparent centrifuge tube (210) is arranged in the positioning groove (209).

4. A biomacromolecule separation system according to claim 3, characterized in that: A first driving device (203) is installed at the lower end of the first mounting plate (202), and the main shaft of the first driving device (203) is connected to the axis of the support plate (204).

5. A biomacromolecule separation system according to claim 3, characterized in that: Symmetrically distributed first fixing plates (205) are installed at the side end of the support plate (204). A second fixing plate (206) is installed at the end of the first fixing plate (205). A spring (207) is installed at the side end of the second fixing plate (206). A damper is arranged in the spring (207), and a positioning plate (208) is installed at the end of the spring (207).

6. The biological macromolecule separation system according to claim 1, wherein: A clamping block (105) is installed at the upper part of the outer surface of the separation box (101). A clamping buckle (106) is installed at the side end of the cover plate (104), and the clamping buckle (106) is clamped on the clamping block (105).

7. A biomacromolecule separation system according to claim 1, characterized in that: A second driving device (304) is installed at the lower end of the lower mounting plate (301), and the main shaft of the second driving device (304) is connected to the axis of the threaded rod (303).

8. A biomacromolecule separation system according to claim 1, characterized in that: A limiting sliding groove is provided on the inner wall of the separation box (101). A limiting sliding block is installed at the rear end of the lifting block (305), and the limiting sliding block is slidably installed in the limiting sliding groove.

9. A biomacromolecule separation system according to claim 1, characterized in that: A discharge pump (401) is provided at the rear end of the separation box (101). A suction pipe (402) and a discharge pipe (403) are provided on the discharge pump (401). The end of the suction pipe (402) is connected to the material taking pipe (307).

Images