Immune cell cryopreservation device

Abstract

The utility model relates to the field of cell cryopreservation, and discloses an immune cell cryopreservation device which comprises a cryopreservation tank, the protection plate is mounted in the cryopreservation tank, the protection plate is used for dividing the interior of the cryopreservation tank into a liquid nitrogen storage area and an operation area, the liquid nitrogen storage area is used for storing liquid nitrogen to cryopreserve immune cells, and the operation area is used for providing a space for operating and storing the immune cells; and the basket mechanisms are mounted on the protection plate and are arranged to be capable of moving on the protection plate. According to the cryopreservation tank, the protection plate is arranged in the cryopreservation tank, so that the direct contact between the operation area and liquid nitrogen is effectively isolated, the evaporation capacity of the liquid nitrogen in the operation process is reduced, the sight definition of operators is improved, and when the lifting basket mechanism is lifted for cell storage and taking operation, the switch mechanism can close the corresponding mounting hole to prevent leakage of the liquid nitrogen.

Description

Technical Field

[0001] The utility model relates to the field of cell freezing, in particular to an immune cell freezing device. Background Art

[0002] Immune cell cryopreservation is a technology that extracts, processes and preserves immune cells from humans or animals so that they can be reused in the future when needed. Blood is drawn from the human body and the immune cells (such as T cells, B cells, NK cells, etc.) in the blood are separated through centrifugation or other separation techniques. The separated immune cells are counted, the concentration is adjusted, and a specific preservation fluid is prepared to ensure the survival rate and quality of the cells during the cryopreservation process. The processed immune cell samples are placed in an ice-salt mixture for pre-cooling, then transferred to liquid nitrogen, and finally stored in an ultra-low temperature liquid nitrogen tank at -196°C.

[0003] When the existing immune cell freezing tank is opened for cell storage and retrieval operations, the liquid nitrogen in the tank will be directly exposed to the air, which will not only cause the liquid nitrogen to evaporate rapidly and produce a large amount of white mist, seriously obstructing the operator's vision and affecting the accuracy and efficiency of the operation, but the direct exposure of liquid nitrogen also poses a safety hazard. Liquid nitrogen is extremely volatile at room temperature and has an extremely low temperature. Direct contact with the skin can cause severe frostbite. When the freezing tank is opened, if the operation is improper or protective measures are insufficient, the operator is extremely vulnerable to injury, exacerbating the safety hazard. Utility Model Content

[0004] In order to solve the above-mentioned problems, the present invention is implemented through the following technical solutions:

[0005] An immune cell freezing device comprises: a freezing tank; a protective plate installed in the freezing tank, the protective plate being used to separate the interior of the freezing tank into a liquid nitrogen storage area and an operating area, wherein the liquid nitrogen storage area is used to store liquid nitrogen for freezing immune cells, and the operating area is used to provide space for operating and storing immune cells; a plurality of basket mechanisms installed on the protective plate and configured to be movable on the protective plate, each basket mechanism being used to store immune cells and being able to move along the protective plate to the operating area for accessing and storing immune cells; a plurality of switch mechanisms installed on the protective plate, each switch mechanism being configured to trigger a closing operation of the protective plate when the corresponding basket mechanism moves upward and disengages from the protective plate, so as to keep the liquid nitrogen storage area closed during operation and prevent leakage of liquid nitrogen, the switch mechanism comprising: a mounting seat installed at the bottom of the protective plate; a connecting seat connected to the mounting seat; a protective cover installed on the connecting seat, the protective cover being configured to close the mounting hole when the storage rack is moved out of the mounting hole.

[0006] The protective plate includes: a plurality of mounting holes opened on the protective plate, and the basket lifting mechanism is arranged in the mounting holes; a plurality of sealing members installed at the bottom of the protective plate and arranged below the mounting holes, and the sealing members are in contact with the basket lifting mechanism and are used to provide sealing for the connection between the mounting holes and the basket lifting mechanism.

[0007] The basket mechanism includes: a storage rack, which is arranged in the mounting hole and contacts the inner ring of the seal; a cover plate, which is installed on the top of the storage rack and fits the cover plate with the protective plate; a plurality of storage boxes, which are connected to the storage rack and are used to store immune cells; a movable groove, which is opened on the storage rack, and a limiting block is connected to the inner wall of the mounting hole, and the limiting block is set in the movable groove; a plurality of limiting grooves, which are opened on the side walls of the movable groove and are used to rotate after the storage rack moves, so that the limiting block enters the limiting groove and limits the position of the storage rack in the mounting hole; a handle, which is installed on the cover plate.

[0008] The mounting seat includes: a groove, which is opened on the mounting seat; a rotating block, which is connected in the groove, and the connecting seat is connected to the rotating block; an elastic member, one end of which is connected to the inner wall of the groove and the other end is connected to the rotating block, and the elastic member is used to provide a restoring force for the rotating block to its initial position.

[0009] The present invention provides an immune cell freezing device. Compared with the prior art, it has the following beneficial effects:

[0010] By installing a protective plate inside the freezing tank, the space inside the tank is divided into a liquid nitrogen storage area and an operating area, effectively isolating the operating area from direct contact with liquid nitrogen, reducing the evaporation of liquid nitrogen during operation, reducing the generation of white mist, improving the operating environment, improving the operator's vision clarity, and improving the accuracy and efficiency of the operation.

[0011] 2. Enhanced safety is achieved through a switch mechanism. When the basket is lifted for cell access, the switch closes the corresponding mounting hole, preventing the escape of liquid nitrogen and avoiding accidental leaks during operation. The basket mechanism is designed with operational convenience in mind. Operators can easily lift and move the rack using the handle, eliminating the need for tools to access cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 This is a schematic diagram of the three-dimensional structure proposed by the utility model.

[0013] Figure 2 This is a schematic structural diagram of the protective plate, basket carrying mechanism and switch mechanism proposed in the utility model.

[0014] Figure 3This is a structural schematic diagram of the basket carrying mechanism proposed in the utility model.

[0015] Figure 4 This is a schematic structural diagram of the protective plate, cover plate, storage rack and storage box proposed in the utility model.

[0016] Figure 5 This is a schematic diagram of the cross-sectional structure of the switch mechanism proposed in the present utility model.

[0017] The reference numerals in the figures are:

[0018] 1. Freezing tank;

[0019] 2. Protective plate; 201. Mounting hole; 202. Limit block; 203. Sealing element;

[0020] 3. Basket mechanism; 301. Cover plate; 302. Storage rack; 303. Storage box; 304. Moving slot; 305. Limiting slot; 306. Handle;

[0021] 4. Switch mechanism; 401. Mounting seat; 402. Connecting seat; 403. Rotating block; 404. Elastic member; 405. Protective cover. DETAILED DESCRIPTION

[0022] To make the purpose, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention are clearly and completely described. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0023] Reference Figure 1-Figure 5, an immune cell freezing device, comprising: a freezing tank 1; a protective plate 2, installed in the freezing tank 1, the protective plate 2 is used to separate the interior of the freezing tank 1 into a liquid nitrogen storage area and an operating area, wherein the liquid nitrogen storage area is used to store liquid nitrogen to freeze immune cells, and the operating area is used to provide space for operating and storing immune cells. The protective plate 2 not only optimizes space utilization, but more importantly, effectively isolates the operating area from direct contact with liquid nitrogen, significantly reducing the generation of fog and safety hazards during operation. The protective plate 2 also serves as an installation platform for a basket lifting mechanism 3 and a switch mechanism 4, providing support for the functional realization of the entire device; a plurality of basket lifting mechanisms 3 are installed on the protective plate 2 and are configured to be movable on the protective plate 2, each basket lifting mechanism The structures 3 are all used to store immune cells and can move along the protective plate 2 to the operating area to perform immune cell storage and access operations; a number of switch mechanisms 4 are installed on the protective plate 2, and each switch mechanism 4 is configured to trigger the closing operation of the protective plate 2 when the corresponding basket mechanism 3 moves upward to disengage from the protective plate 2, so as to keep the liquid nitrogen storage area closed during operation to prevent liquid nitrogen from leaking. The switch mechanism 4 includes: a mounting seat 401, installed at the bottom of the protective plate 2; a connecting seat 402, connected to the mounting seat 401; a protective cover 405, installed on the connecting seat 402, and the protective cover 405 is configured to close the mounting hole 201 when the storage rack 302 is moved out of the mounting hole 201.

[0024] Reference Figure 4 The protective plate 2 includes: a plurality of mounting holes 201, which are opened on the protective plate 2, and the basket lifting mechanism 3 is arranged in the mounting holes 201; a plurality of sealing members 203, which are installed at the bottom of the protective plate 2 and are arranged below the mounting holes 201. The sealing members 203 are in contact with the basket lifting mechanism 3 and are used to provide sealing for the connection between the mounting holes 201 and the basket lifting mechanism 3.

[0025] Reference Figure 3 and Figure 4The basket mechanism 3 includes: a storage rack 302, which is arranged in the mounting hole 201, and the storage rack 302 is in contact with the inner ring of the seal 203. The storage rack 302 is in close contact with the seal 203, ensuring the sealing of the connection between the mounting hole 201 and the basket mechanism 3, preventing leakage of liquid nitrogen, ensuring the stability of the liquid nitrogen storage area, and also protecting the immune cells in the storage rack 302 from external contamination; a cover plate 301, which is installed on the top of the storage rack 302, and the cover plate 301 is in contact with the protective plate 2. The cover plate 301 provides additional protection for the storage rack 302, further enhancing the sealing effect, and the storage box 303 is directly used to store immune cells. Its structural design is reasonable, which is convenient for taking, placing and storing immune cells; a plurality of storage boxes 303 are connected to the storage rack 302, and the storage boxes 303 are used to store immune cells; a movable slot 304 is opened On the storage rack 302, a limiting block 202 is connected to the inner wall of the mounting hole 201, and the limiting block 202 is arranged in the movable groove 304; a plurality of limiting grooves 305 are opened on the side wall of the movable groove 304, which is used to rotate after the storage rack 302 moves, so that the limiting block 202 enters the limiting groove 305, limiting the position of the storage rack 302 in the mounting hole 201; a handle 306 is installed on the cover plate 301. The combination of the movable groove 304 and the limiting block 202 enables the storage rack 302 to move smoothly on the protective plate 2, and the design of the limiting groove 305 ensures that the storage rack 302 can be firmly fixed after moving to the designated position, preventing damage or leakage of immune cells due to misoperation. The operator can easily lift and move the storage rack 302 through the handle 306 without using other tools, thereby improving work efficiency and safety.

[0026] Reference Figure 5 The mounting seat 401 includes: a groove, which is opened on the mounting seat 401; a rotating block 403, which is connected in the groove, and the connecting seat 402 is connected to the rotating block 403; an elastic member 404, one end of which is connected to the inner wall of the groove, and the other end is connected to the rotating block 403. The elastic member 404 is used to provide a restoring force for the initial position of the rotating block 403. The elastic member 404 adopts a clockwork spring or a coil spring. When the storage rack 302 is moved out of the mounting hole 201, the protective cover 405 will automatically close the mounting hole 201, effectively preventing liquid nitrogen from leaking during operation, ensuring the sealing and safety of the liquid nitrogen storage area. The elastic member 404 made of elastic materials such as a clockwork spring or a coil spring provides a restoring force for the initial position of the rotating block 403, ensuring that the protective cover 405 can automatically reset without human intervention, thereby improving the automation and reliability of the device.

[0027] During use, after opening the freezing tank 1, the operator lifts the storage rack 302 by the handle 306, so that the storage rack 302 moves upward along the moving groove 304. As the storage rack 302 rises, the operator can move the storage rack 302 to the operation area to facilitate the storage operation of the immune cells. Then, by rotating the storage rack 302, the limiting block 202 is stuck in the limiting groove 305, the storage rack 302 is limited in the installation hole 201, and the tube body containing the immune cells is placed in the storage box 303. If it continues to move, the operator can move the storage rack 302 to the operation area to facilitate the storage operation of the immune cells. When the storage rack 302 is completely moved out of the mounting hole 201 and is in the operating area, the protective cover 405 in the corresponding switch mechanism 4 automatically closes the mounting hole 201 under the action of the elastic member 404 to prevent the leakage of liquid nitrogen. After the storage operation is completed, the operator puts the storage rack 302 back into the mounting hole 201. The storage rack 302 can squeeze the protective cover 405, causing the protective cover 405 to rotate and open the mounting hole 201. The storage rack 302 is placed in the freezing tank 1, and the cover plate 301 is close to the protective plate 2.

[0028] In summary, compared with the existing technology, it has the following beneficial effects:

[0029] By setting a protective plate 2 in the freezing tank 1, the space inside the tank is divided into a liquid nitrogen storage area and an operating area, which effectively isolates the operating area from direct contact with liquid nitrogen, reduces the evaporation of liquid nitrogen during operation, reduces the generation of white mist, improves the operating environment, improves the operator's visual clarity, and improves the accuracy and efficiency of operation.

[0030] Safety is enhanced by the switch mechanism 4. When the basket mechanism 3 is lifted for cell access, the switch mechanism 4 closes the corresponding mounting hole 201 to prevent leakage of liquid nitrogen and avoid accidental leakage of liquid nitrogen during operation. The design of the basket mechanism 3 fully considers the convenience of operation. The operator can easily lift and move the storage rack 302 using the handle 306 and complete the cell access operation without using other tools.

[0031] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply the existence of any such actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article, or device comprising a series of elements includes not only those elements, but also other elements not explicitly listed, or elements inherent to such process, method, article, or device. In the absence of further limitations, an element defined by the phrase "comprising a ..." does not exclude the presence of other identical elements in the process, method, article, or device comprising the element.

[0032] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments can still be modified, or some of the technical features thereof can be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. An immune cell freezing device, characterized in that: include: Freezing tank (1); A protective plate (2) is installed in the freezing tank (1), and the protective plate (2) is used to separate the interior of the freezing tank (1) into a liquid nitrogen storage area and an operation area, wherein the liquid nitrogen storage area is used to store liquid nitrogen for freezing immune cells, and the operation area is used to provide a space for operating and storing immune cells; A plurality of basket mechanisms (3) are mounted on the protective plate (2) and are configured to be movable on the protective plate (2); each basket mechanism (3) is used to store immune cells and is capable of moving along the protective plate (2) to an operating area to perform immune cell storage and retrieval operations; A plurality of switch mechanisms (4) are mounted on the protective plate (2), each switch mechanism (4) being configured to trigger a closing operation of the protective plate (2) when the corresponding basket lifting mechanism (3) moves upwards and is out of contact with the protective plate (2), so as to keep the liquid nitrogen storage area closed during operation to prevent leakage of the liquid nitrogen, wherein the switch mechanism (4) comprises: A mounting seat (401) is mounted on the bottom of the protective plate (2); A connecting seat (402) connected to the mounting seat (401); A protective cover (405) is mounted on the connecting seat (402), and the protective cover (405) is configured to close the mounting hole (201) when the storage rack (302) moves out of the mounting hole (201).

2. The immune cell freezing device according to claim 1, characterized in that: The protective plate (2) comprises: A plurality of mounting holes (201) are provided on the protective plate (2), and the basket lifting mechanism (3) is arranged in the mounting holes (201); A plurality of sealing members (203) are mounted on the bottom of the protective plate (2) and are arranged below the mounting hole (201). The sealing members (203) are in contact with the basket lifting mechanism (3) and are used to provide sealing for the connection between the mounting hole (201) and the basket lifting mechanism (3).

3. The immune cell freezing device according to claim 2, characterized in that: The basket carrying mechanism (3) comprises: A storage rack (302) is arranged in the mounting hole (201), and the storage rack (302) is in contact with the inner ring of the sealing member (203); A cover plate (301) is mounted on the top of the storage rack (302), and the cover plate (301) is in contact with the protective plate (2); A plurality of storage boxes (303) are connected to the storage rack (302), and the storage boxes (303) are used to store immune cells.

4. The immune cell freezing device according to claim 3, characterized in that: The basket carrying mechanism (3) further comprises: A movable groove (304) is provided on the storage rack (302), a limiting block (202) is connected to the inner wall of the mounting hole (201), and the limiting block (202) is provided in the movable groove (304); A plurality of limiting grooves (305) are provided on the side walls of the movable groove (304) and are used for rotating after the storage rack (302) moves, so that the limiting block (202) enters the limiting groove (305) and limits the position of the storage rack (302) in the mounting hole (201).

5. The immune cell freezing device according to claim 3, characterized in that: The basket carrying mechanism (3) further comprises: A handle (306) is mounted on the cover plate (301).

6. The immune cell freezing device according to claim 1, characterized in that: The mounting seat (401) comprises: A groove is formed on the mounting seat (401); The rotating block (403) is connected in the groove, and the connecting seat (402) is connected to the rotating block (403).

7. The immune cell freezing device according to claim 6, characterized in that: The mounting seat (401) further comprises: An elastic member (404) has one end connected to the inner wall of the groove and the other end connected to the rotating block (403), and the elastic member (404) is used to provide a restoring force for the rotating block (403) to return to its initial position.

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