Separation and purification device for extracting lentivirus

Abstract

The utility model relates to the technical field of separation and purification devices, and discloses a separation and purification device for extracting lentivirus, which comprises a filter cartridge, a placement rack detachably mounted in the filter cartridge, a clamping cover mounted at the end part of the filter cartridge, a rotating button mounted in the clamping cover, and a rotating component arranged at the bottom of the rotating button, a clamping block is arranged on the side portion of the rotating assembly, a clamping groove is formed in the side portion of the filter cartridge, the clamping block and the clamping groove are clamped in a sliding mode, the clamping cover and the filter cartridge are clamped through the arrangement of the clamping cover, the rotating button is pressed, the rotating button drives the rotating rod, and the rotating rod rotates and shrinks; meanwhile, a sliding block and a connecting rod at the side part of the sliding block are driven, a sliding rod slides in a fixed cylinder, so that the sliding rod drives a clamping block to be clamped with a clamping groove in a sliding manner, and the clamping block made of a rubber material has good elasticity and compressibility and can be tightly attached to a contact surface between the filter cylinder and a clamping cover, so that a gap is reduced; and leakage of air or pollutants is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of separation and purification devices, in particular to a separation and purification device for extracting lentiviruses. Background Art

[0002] Lentivirus isolation and purification systems typically involve several key components for isolating and purifying lentiviruses from complex biological samples. Prior to virus isolation, the sample requires pretreatment to remove cell debris and other impurities. Common pretreatment methods include centrifugation and filtration. Centrifugation separates cell debris from the virus through high-speed rotation, while filtration removes larger particles through a membrane filter. Virus isolation systems typically utilize density gradient centrifugation or chromatography. Density gradient centrifugation utilizes layers of media of varying densities, allowing the virus to precipitate at specific levels. Chromatographic techniques such as gel filtration, affinity chromatography, and ion exchange chromatography can separate viruses based on their physicochemical properties. Purification steps are designed to further remove any remaining impurities. Common methods include affinity chromatography, which utilizes specific ligands to bind to viral surface proteins, achieving highly specific purification. Ultrafiltration can also be used to concentrate and remove small molecule impurities. In order to ensure the effectiveness of separation and purification, various detection equipment are usually equipped, such as enzyme-linked immunosorbent assay (ELISA), real-time quantitative PCR (qPCR) and electron microscope to detect the purity and concentration of the virus. Modern equipment is usually equipped with an automated system that can automatically complete the sample processing, separation and purification process.

[0003] Currently, poor sealing during lentivirus filtration can allow air or other exogenous contaminants to enter the filtration system. This can lead to sample contamination, affecting virus purity and experimental results, and may even introduce other viruses or microorganisms. Therefore, this does not meet existing needs. To address this, we have proposed a separation and purification device for lentivirus extraction. Utility Model Content

[0004] The present invention provides a separation and purification device for extracting lentiviruses. The device features a snap-fit block with excellent elasticity and compressibility, enabling it to tightly adhere to the contact surface between the filter cartridge and the snap-fit cover, thereby reducing gaps and preventing the leakage of air or contaminants. This device addresses the aforementioned issue in the background art regarding the potential for air or other exogenous contaminants to enter the filtration system during lentivirus filtration due to poor sealing. This can lead to sample contamination, affecting virus purity and experimental results, and even potentially introducing other viruses or microorganisms.

[0005] The utility model provides the following technical solution: a separation and purification device for extracting lentivirus, comprising a filter cartridge, a placement rack detachably installed inside the filter cartridge, a clamping cover installed at the end of the filter cartridge, a rotating knob installed inside the clamping cover, a rotating assembly provided at the bottom of the rotating knob, a clamping block provided on the side of the rotating assembly, a clamping groove provided on the side of the filter cartridge, and the clamping block slidably engaged with the clamping groove.

[0006] As an optional solution of the separation and purification device for extracting lentiviruses described in the present invention, the placement rack includes an integrated rack and a piston disc, and a plurality of the piston discs are provided, and the plurality of the piston discs are connected to the side of the integrated rack.

[0007] As an optional solution of the separation and purification device for extracting lentiviruses described in the present invention, a slide groove is opened on the inner wall of the filter cylinder, and a plurality of the piston discs are slidably engaged with the slide groove.

[0008] As an optional solution of the separation and purification device for extracting lentiviruses described in the present invention, the rotating assembly includes a fixed plate and a fixed cylinder, the side of the fixed plate is connected to a fixed shaft, the fixed shaft is connected to the bottom of the clamping cover, and the fixed cylinder is connected to the bottom of the fixed plate.

[0009] As an optional solution of the separation and purification device for extracting lentiviruses described in the present invention, wherein: both ends of the fixed cylinder are slidably connected to sliding rods, and the ends of the sliding rods are provided with connecting rods.

[0010] As an optional solution of the separation and purification device for extracting lentiviruses described in the present invention, the engaging block is connected to the end of the sliding rod, the surface of the fixed plate is connected to a rotating shaft, and a rotating rod is sleeved on the outer side of the rotating shaft.

[0011] As an optional solution of the separation and purification device for extracting lentiviruses described in the utility model, wherein: the end of the rotating rod is connected to a sliding block, the rotating knob is connected to the surface of the sliding block, the surface of the clamping cover is provided with a notch, and the rotating knob slidably cooperates with the notch.

[0012] As an optional solution of the separation and purification device for extracting lentiviruses described in the present invention, wherein: a discharge port is opened at the bottom of the filter cartridge, and a pull ring is connected to the end of the placement rack.

[0013] The utility model has the following beneficial effects:

[0014] 1. This separation and purification device for extracting lentiviruses uses a clamping cover to engage the clamping cover with the filter cartridge. Pressing the rotating knob drives the rotating rod, which rotates and contracts, and at the same time drives the sliding block and the connecting rod and sliding rod on the side of the sliding block to slide inside the fixed cartridge. The sliding rod drives the clamping block to slide and engage with the clamping groove. The rubber clamping block has good elasticity and compressibility, and can tightly fit the contact surface between the filter cartridge and the clamping cover, thereby reducing gaps and preventing leakage of air or contaminants. This solves the problem that poor sealing during lentivirus filtration may cause air or other exogenous contaminants to enter the filtration system. This can lead to sample contamination, affect the purity of the virus and experimental results, and may even introduce other viruses or microorganisms.

[0015] 2. This lentivirus separation and purification device features a mounting rack that works in conjunction with the piston disc. When disassembly is required, a pull ring is pulled to disassemble the mounting rack. The piston disc and the slideway work together, and the pull ring triggers the disassembly mechanism, simplifying the disassembly process and reducing tool dependency and operational complexity. This is crucial for regular cleaning, component replacement, or maintenance, saving time and simplifying operations. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the three-dimensional structure of the utility model.

[0017] Figure 2 It is a schematic diagram of the cutaway structure of the present utility model.

[0018] Figure 3 For this utility model Figure 2 A is an enlarged structural diagram of FIG.

[0019] Figure 4 This is a schematic diagram of the fixed plate structure of the present utility model.

[0020] In the figure: 110, filter cartridge; 120, placement rack; 121, integrated frame; 122, piston disc; 123, slide groove; 130, clamping cover; 131, rotating knob; 134, locking block; 135, locking groove; 140, rotating assembly; 141, fixed plate; 142, fixed cartridge; 143, fixed shaft; 144, sliding rod; 145, connecting rod; 150, rotating shaft; 151, rotating rod; 152, sliding block; 154, notch; 155, discharge port; 160, pull ring. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0022] Example 1: This example aims to solve the problem that poor sealing during lentivirus filtration may cause air or other exogenous contaminants to enter the filtration system. This may lead to sample contamination, affect the purity of the virus and experimental results, and may even introduce other viruses or microorganisms. Please refer to Figure 1-4 A separation and purification device for extracting lentiviruses includes a filter cartridge 110, a placement rack 120 is detachably installed inside the filter cartridge 110, a clamping cover 130 is also installed at the end of the filter cartridge 110, a rotating button 131 is installed inside the clamping cover 130, a rotating assembly 140 is provided at the bottom of the rotating button 131, a clamping block 134 is provided on the side of the rotating assembly 140, and a clamping groove 135 is opened on the side of the filter cartridge 110, and the clamping block 134 is slidably engaged with the clamping groove 135.

[0023] The placement rack 120 includes an integrated rack 121 and a piston disc 122 . There are several piston discs 122 connected to the side of the integrated rack 121 . A slide groove 123 is opened on the inner wall of the filter cartridge 110 , and the several piston discs 122 slide in cooperation with the slide groove 123 .

[0024] The rotating assembly 140 includes a fixed plate 141 and a fixed cylinder 142. The side of the fixed plate 141 is connected to a fixed shaft 143, the fixed shaft 143 is connected to the bottom of the clamping cover 130, the fixed cylinder 142 is connected to the bottom of the fixed plate 141, and the two ends of the fixed cylinder 142 are respectively slidably connected to sliding rods 144, and a connecting rod 145 is provided at the end of the sliding rod 144.

[0025] In this embodiment, the clamping cover 130 is provided to engage the clamping cover 130 with the filter cartridge 110, and the rotating knob 131 is pressed, which drives the rotating rod 151, which rotates and contracts, and at the same time drives the sliding block 152 and the connecting rod 145 and sliding rod 144 on the side of the sliding block 152 to slide inside the fixed cylinder 142, so that the sliding rod 144 drives the locking block 134 to slide and engage with the locking groove 135. The locking block 134 made of rubber has good elasticity and compressibility, and can tightly fit the contact surface between the filter cartridge 110 and the clamping cover 130, thereby reducing gaps and preventing the leakage of air or contaminants. This solves the problem that poor sealing may cause air or other exogenous contaminants to enter the filtration system during lentivirus filtration. This may lead to sample contamination, affect the purity of the virus and experimental results, and may even introduce other viruses or microorganisms.

[0026] Example 2: This example is intended to promote the solution of the problem. This example is an improvement made on the basis of Example 1. For details, please refer to Figure 1-4 The locking block 134 is connected to the end of the sliding rod 144, the surface of the fixed plate 141 is connected to the rotating shaft 150, the outer side of the rotating shaft 150 is sleeved with a rotating rod 151, the locking block 134 is set as a rubber block, the end of the rotating rod 151 is connected to the sliding block 152, the rotating button 131 is connected to the surface of the sliding block 152, the surface of the clamping cover 130 is provided with a notch 154, the rotating button 131 slides with the notch 154, the bottom of the filter cartridge 110 is provided with a discharge port 155, and the end of the placement rack 120 is connected to a pull ring 160.

[0027] In this embodiment, the placement rack 120 is provided, and the integrated frame 121 cooperates with the piston disc 122. When disassembly is required, the pull ring 160 is pulled to disassemble the placement rack 120. The piston disc 122 cooperates with the slide 123, and the disassembly mechanism triggered by pulling the pull ring 160 simplifies the disassembly process, reducing tool dependency and operational complexity. This is crucial for regular cleaning, component replacement, or maintenance, saving time and simplifying operations.

[0028] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus.

[0029] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A separation and purification device for extracting lentivirus, comprising a filter cartridge (110), characterized in that: A placement rack (120) is detachably mounted inside the filter cartridge (110), a clamping cover (130) is also mounted on the end of the filter cartridge (110), a rotating knob (131) is mounted inside the clamping cover (130), a rotating assembly (140) is disposed at the bottom of the rotating knob (131), a locking block (134) is disposed on the side of the rotating assembly (140), a locking groove (135) is provided on the side of the filter cartridge (110), and the locking block (134) is slidably engaged with the locking groove (135).

2. The device for separating and purifying lentivirus according to claim 1, characterized in that: The placement rack (120) comprises an integrated rack (121) and a piston disc (122). A plurality of the piston discs (122) are provided, and the plurality of the piston discs (122) are connected to the side of the integrated rack (121).

3. The separation and purification device for extracting lentivirus according to claim 2, characterized in that: A sliding groove (123) is provided on the inner wall of the filter cylinder (110), and a plurality of piston discs (122) are slidably engaged with the sliding groove (123).

4. The device for separating and purifying lentivirus according to claim 3, characterized in that: The rotating assembly (140) comprises a fixed plate (141) and a fixed cylinder (142), the fixed plate (141) is connected to a fixed shaft (143) on its side, the fixed shaft (143) is connected to the bottom of the clamping cover (130), and the fixed cylinder (142) is connected to the bottom of the fixed plate (141).

5. The separation and purification device for extracting lentivirus according to claim 4, characterized in that: Both ends of the fixed cylinder (142) are slidably connected to sliding rods (144), and the ends of the sliding rods (144) are provided with connecting rods (145).

6. The device for separating and purifying lentivirus according to claim 5, characterized in that: The engaging block (134) is connected to the end of the sliding rod (144); a rotating shaft (150) is connected to the surface of the fixing plate (141); and a rotating rod (151) is sleeved on the outside of the rotating shaft (150).

7. The separation and purification device for extracting lentivirus according to claim 6, characterized in that: The end of the rotating rod (151) is connected to a sliding block (152), the rotating button (131) is connected to the surface of the sliding block (152), a notch (154) is provided on the surface of the clamping cover (130), and the rotating button (131) is slidably engaged with the notch (154).

8. The device for separating and purifying lentivirus according to claim 7, characterized in that: A discharge port (155) is provided at the bottom of the filter cartridge (110), and a pull ring (160) is connected to the end of the placement rack (120).

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