Photosynthetic organism cultivation device

Abstract

The utility model provides a photosynthetic organism cultivation device. The photosynthetic organism cultivation device comprises at least two cultivation units which are arranged in a stacked mode. Each cultivation unit comprises a shell, a plurality of cultivation units and a plurality of cultivation units, mounting holes are formed in the shells, and the shells are made of shading materials; the culture dish is positioned in the mounting hole and is used for accommodating photosynthetic organisms; the illumination assembly is located in the mounting hole and at least comprises a first light source and a second light source, and the first light source and the second light source are used for emitting illumination light with different wavelengths; the light guide assembly is located in the mounting hole, located on a light path of the illumination light and used for diffusing the illumination light and guiding the diffused illumination light to the culture dish; and the control circuit is electrically connected with the first light source and the second light source respectively and is used for controlling the on-off state and the luminous intensity of the first light source and the second light source.

Description

Technical Field

[0001] The present application relates to a photosynthetic organism cultivation device for use in a laboratory. Background Art

[0002] The light source of traditional laboratory culture dish lighting systems is difficult to meet the cultivation needs of different photosynthetic organisms. Utility Model Content

[0003] The present application provides a photosynthetic organism cultivation device, comprising at least two cultivation units arranged in a stacked manner; each of the cultivation units comprises: a shell having a mounting hole formed therein, the shell being made of a light-shielding material; a culture dish located in the mounting hole and used to accommodate the photosynthetic organism; an illumination assembly located in the mounting hole and comprising at least a first light source and a second light source, the first light source and the second light source being used to emit illumination light of different wavelengths; a light guide assembly located in the mounting hole and on an optical path of the illumination light, and being used to diffuse the illumination light and guide the diffused illumination light to the culture dish; and a control circuit electrically connected to the first light source and the second light source, respectively, and being used to control the switching state and luminous intensity of the first light source and the second light source.

[0004] In at least one embodiment of the present application, each of the cultivation units further includes a magnetic structure fixed to the shell, and the magnetic structure is configured to magnetically attract the magnetic structure in an adjacently arranged cultivation unit to connect the at least two cultivation units.

[0005] In at least one embodiment of the present application, the shell includes a first surface and a second surface parallel to each other, and the mounting hole passes through the first surface and the second surface; the photosynthetic organism cultivation device includes two magnetic structures, one of which is exposed relative to the first surface, and the other is exposed relative to the second surface.

[0006] In at least one embodiment of the present application, the magnetic attraction structure includes a first contact terminal and a second contact terminal spaced apart from each other, one of the first contact terminal and the second contact terminal serving as a positive electrode and the other serving as a negative electrode, and both are electrically connected to the control circuit.

[0007] In at least one embodiment of the present application, the first contact terminal surrounds the second contact terminal.

[0008] In at least one embodiment of the present application, the housing further includes a light shielding plate, which covers the opening of the mounting hole on the first surface; the lighting assembly and the light guide assembly are located between the light shielding plate and the culture dish.

[0009] In at least one embodiment of the present application, the light guide assembly includes a reflective sheet, a light guide plate and a diffuser that are stacked in sequence, and the diffuser is closer to the culture dish than the reflective sheet; the reflective sheet is used to reflect the received illumination light, and the diffuser is used to diffuse the illumination light from the light guide plate.

[0010] In at least one embodiment of the present application, the light guide plate has a circular planar structure, and the first light source and the second light source are spaced and symmetrically arranged along the circumference of the light guide plate.

[0011] In at least one embodiment of the present application, the light emitting surfaces of the first light source and the second light source are attached to the light guide plate.

[0012] In at least one embodiment of the present application, each of the cultivation units further includes a first adjustment knob and a second adjustment knob movably connected to the shell, and by rotating the first adjustment knob and the second adjustment knob, the luminous intensity of the first light source and the second light source can be adjusted respectively.

[0013] The photosynthetic organism cultivation device of the embodiment of the present application can control the switching and luminous intensity of the first light source and the second light source in each lighting component by the control circuit, so that each culture dish can receive illumination light of different light qualities, thereby simulating various lighting conditions, observing the growth of photosynthetic organisms in the culture dish under various lighting conditions, and thus screening out the more optimal lighting conditions required for specific types of photosynthetic organisms. On the other hand, the photosynthetic organism cultivation device can allocate illumination light of different light qualities for different types of photosynthetic organisms, so that the photosynthetic organism cultivation device can be applicable to the cultivation process of multiple different types of photosynthetic organisms. Furthermore, the present application adopts a magnetic suction method to connect two adjacent cultivation units. The culture dish can be conveniently taken in and out by simply overcoming the magnetic force and taking the shell. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 3D diagram of the structure of the photosynthetic organism cultivation device in the embodiment of this application.

[0015] Figure 2 for Figure 1 Exploded structure diagram of a single cultivation unit.

[0016] Figure 3 for Figure 1 Schematic diagram of the cross-sectional structure of the photosynthetic organism cultivation device along line III-III.

[0017] Figure 4 for Figure 1 Schematic diagram of the cross-sectional structure of the photosynthetic organism cultivation device along line IV-IV.

[0018] Description of main component symbols

[0019] Photosynthetic organism cultivation device 100

[0020] Cultivation Unit 10

[0021] Housing 11

[0022] First surface 111

[0023] Second surface 112

[0024] Third surface 113

[0025] Fourth surface 114

[0026] Mounting hole 115

[0027] Shade 116

[0028] Ventilation 117

[0029] Power interface 118

[0030] Magnetic structure 12

[0031] First contact terminal 121

[0032] Second contact terminal 122

[0033] Locking screw 123

[0034] Connecting plate 124

[0035] Petri dish 13

[0036] First loading part 131

[0037] Second loading part 132

[0038] Lighting Component 14

[0039] First light source 141

[0040] Second light source 142

[0041] Light guide component 15

[0042] Reflective sheet 151

[0043] Light guide plate 152

[0044] Diffuser 153

[0045] Control circuit 16

[0046] First adjustment knob 171

[0047] Second adjustment knob 172

[0048] The following specific implementation methods will further illustrate the present application in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION

[0049] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments.

[0050] Unless otherwise defined, all technical and scientific terms used in this application have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application.

[0051] In order to further illustrate the technical means and effects adopted by this application to achieve the intended purpose, the following detailed description of this application is made in conjunction with the accompanying drawings and preferred implementation methods.

[0052] Photosynthetic organisms (such as plants and microalgae) have the ability to photosynthesize and can be widely used in the fields of medicine, energy, environment, food, etc. By cultivating and observing photosynthetic organisms in the laboratory, it is helpful to optimize the cultivation conditions of photosynthetic organisms. The present application provides a photosynthetic organism cultivation device for cultivating photosynthetic organisms in a laboratory scenario. The above-mentioned photosynthetic organism cultivation device, on the one hand, can realize flexible simulation of different lighting conditions, which is conducive to meeting the personalized cultivation needs of different types of photosynthetic organisms; on the other hand, by adjusting different light quality ratios, microbial strains that are adapted to various culture conditions can be screened out to adapt to specific lighting environments or conduct research on the mechanism of photosynthetic pathways under different light qualities; on the third hand, adjacent cultivation units are connected by magnetic attraction, which can facilitate the removal and placement of culture dishes.

[0053] See also Figure 1 The photosynthetic organism cultivation device 100 of the embodiment of the present application includes a plurality of cultivation units 10 arranged in a stacked manner. Each cultivation unit 10 is connected in sequence by magnetic attraction and is used to independently cultivate photosynthetic organisms. In this embodiment, each cultivation unit 10 has substantially the same structure and function.

[0054] Please also refer to Figure 2-Figure 4 In this embodiment, each cultivation unit 10 includes a housing 11, a magnetic structure 12, a culture dish 13, a lighting assembly 14, a light guide assembly 15, and a control circuit 16. The magnetic structure 12, lighting assembly 14, light guide assembly 15, and control circuit 16 are fixedly mounted on the housing 11, and the culture dish 13 is placed inside the housing 11.

[0055] The housing 11 has a first surface 111, a second surface 112, a third surface 113, and a fourth surface 114. The first surface 111 and the second surface 112 are planar and parallel to each other. The third surface 113 connects between the first surface 111 and the second surface 112 and connects the edges of the first surface 111 and the second surface 112. The third surface 113 is perpendicular to the first surface 111 and the second surface 112. The housing 11 has a mounting hole 115 formed therein, extending through the first surface 111 and the second surface 112. The wall of the mounting hole 115 constitutes the fourth surface 114. The housing 11 also includes a light shielding plate 116. The light shielding plate 116 covers the opening formed by the mounting hole 115 on the first surface 111. The housing 11 also has a plurality of ventilation openings 117 formed on the third surface 113. In this embodiment, the housing 11 is entirely made of a light-shielding material, which serves to block ambient light and to isolate the light within each cultivation unit 10, thereby preventing light crosstalk between the cultivation units 10.

[0056] The magnetic structure 12 is embedded in the housing 11. In this embodiment, each cultivation unit 10 includes two magnetic structures 12, one of which is embedded in the first surface 111 and exposed relative to the first surface 111, and the other is embedded in the second surface 112 and exposed relative to the second surface 112. In this way, each cultivation unit 10 can be magnetically connected to two adjacent cultivation units 10 above and below it via the two magnetic structures 12.

[0057] In this embodiment, each magnetic structure 12 includes a first contact terminal 121 and a second contact terminal 122 that are spaced apart. The first contact terminal 121 is a circular sheet-shaped conductive structure. The second contact terminal 122 is a cylindrical conductive structure as a whole and has an annular cross-section. The second contact terminal 122 surrounds the first contact terminal 121. The first contact terminal 121 and the second contact terminal 122 are exposed relative to the first surface 111 and the second surface 112. In two adjacent incubation units 10, the first contact terminal 121 on the first surface 111 of one is magnetically connected and electrically contacted with the first contact terminal 121 on the second surface 112 of the other, and the second contact terminal 122 on the first surface 111 of one is magnetically connected and electrically contacted with the second contact terminal 122 on the second surface 112 of the other.

[0058] In this embodiment, one of the first contact terminal 121 and the second contact terminal 122 serves as the positive electrode, and the other serves as the negative electrode. The housing 11 of one of the incubation units 10 (in this embodiment, the end incubation unit 10) also includes a power interface 118. The magnetic structures 12 in each incubation unit 10 are connected sequentially. By disposing a wire within the housing 11, each incubation unit 10 is powered when the power interface 118 is connected to an external power source. In other words, in this embodiment, the magnetic structure 12 serves both to achieve magnetic connection between incubation units 10 and to power each incubation unit 10.

[0059] In this embodiment, each magnetic structure 12 further includes two locking screws 123 and a connecting plate 124. The two locking screws 123 are located on either side of the first contact terminal 121 and the second contact terminal 122, respectively. The first contact terminal 121 and the second contact terminal 122 are fixed to the connecting plate 124, and the two locking screws 123 are used to fix the connecting plate 124 to the housing 11.

[0060] The culture dishes 13 are used to house and grow photosynthetic organisms. Each dish 13 is constructed entirely of light-transmitting material, allowing the organisms within to receive light and facilitating observation of their growth. The dishes 13 include a first loading member 131 and a second loading member 132. The second loading member 132 engages with the first loading member 131, sealing the dish 13.

[0061] The culture dish 13, illumination assembly 14, and light guide assembly 15 are all located within the mounting hole 115. The wall of the mounting hole 115 (i.e., fourth surface 114) forms a stepped structure, allowing the illumination assembly 14 and light guide assembly 15 to be secured between the stepped structure and the light shielding plate 116. The illumination assembly 14 and light guide assembly 15 are also located between the culture dish 13 and the light shielding plate 116, and are positioned on the side of the culture dish 13 where the second loading member 132 is located.

[0062] When in use, the photosynthetic organism cultivation device 100 can be placed on a flat surface, such as a table or the floor. The cultivation units 10 are stacked vertically. In each cultivation unit 10, the illumination assembly 14 and light guide assembly 15 are positioned above the culture dish 13, and the second loading member 132 is positioned above the first loading member 131. The culture dish 13 is supported on the light shield 116 of the cultivation unit 10 below the cultivation unit 10. Illuminating light emitted by the light guide assembly 15 is directly incident on the culture dish 13 through the second loading member 132, providing illumination for the photosynthetic organisms.

[0063] In this embodiment, an inverted cultivation method is used to cultivate photosynthetic organisms. Specifically, when photosynthetic organisms are cultivated in the culture dish 13, they adhere to the inner wall of the second carrier 132. Because water droplets often form on the inner wall of the first carrier 131, incident illumination light from the side of the second carrier 132 effectively prevents the illumination light from being refracted by the water droplets and then irradiating the photosynthetic organisms, thus minimizing the impact on the illumination effect.

[0064] In other embodiments of the present application, when the photosynthetic organism cultivation device 100 is in use, the illumination assembly 14 and the light guide assembly 15 may also be located below the culture dish 13 , so that the light and organisms in the culture dish 13 can be directly observed from above.

[0065] In this embodiment, in each cultivation unit 10 , the illumination component 14 is disposed on the light guide component 15 for emitting illumination light toward the light guide component 15 ; the light guide component 15 is used to diffuse the illumination light and guide it into the culture dish 13 to illuminate the photosynthetic organisms growing in the culture dish 13 .

[0066] In this embodiment, each illumination assembly 14 includes at least a first light source 141 and a second light source 142. The first light source 141 and the second light source 142 are configured to emit illumination light of different wavelengths. In this embodiment, one of the first light source 141 and the second light source 142 is configured to emit white light, and the other is configured to emit infrared light with a wavelength of 730 nm. In this embodiment, both the first light source 141 and the second light source 142 are light emitting diode (LED) arrays, each comprising a plurality of LEDs.

[0067] The present application does not limit the number of light sources and the wavelength of the illumination light in the illumination component 14. In other embodiments of the present application, the illumination component 14 may include multiple (greater than or equal to three) light sources, wherein the illumination light emitted by at least two light sources has different wavelengths, or the illumination light emitted by each light source may have different wavelengths. In addition to the white light and infrared light described in this embodiment, the illumination light emitted by the light source may also be selected from, for example, blue light (wavelength 40nm0-500nm), red light (wavelength 600nm-700nm), green light (wavelength 500nm-600nm), far infrared light (wavelength 700nm-750nm), ultraviolet light (wavelength 300nm-400nm), etc.

[0068] In this embodiment, each light guide component 15 includes a reflective sheet 151, a light guide plate 152, and a diffuser 153 stacked in sequence, wherein the diffuser 153 is closer to the culture dish 13 than the reflective sheet 151. The planar structure of the reflective sheet 151, the light guide plate 152, and the diffuser 153 is adapted to the shape of the culture dish 13. In this embodiment, the reflective sheet 151, the light guide plate 152, and the diffuser 153 have a roughly circular planar structure. The light sources in the illumination component 14 (including the first light source 141 and the second light source 142 in this embodiment) are spaced and symmetrically arranged along the circumference of the light guide plate 152. The light-emitting surfaces of the first light source 141 and the second light source 142 are in close contact with the side surfaces of the light guide plate 152.

[0069] The illumination light emitted by the first light source 141 and the second light source 142 is incident on the light guide plate 152. The dotting method of the light guide plate 152 is low density at the edges and high density in the middle, which is conducive to uniform illumination light. When light tends to propagate toward the bottom, the reflective sheet 151 is used to reflect it back to the light guide plate 152 for reuse, which can effectively avoid waste of light energy and help improve light utilization. The diffuser 153 is used to diffuse the illumination light from the light guide plate 152 and evenly emit it to the culture dish 13, so as to prevent the illumination light from directly hitting the photosynthetic organisms in the culture dish 13 and affecting their growth. In this way, the light guide assembly 15 in this embodiment is equivalent to a light source, which can emit illumination light with uniform intensity distribution toward the culture dish 13.

[0070] In this embodiment, a hollow space is formed between the third surface 113 and the fourth surface 114 of the housing 11 for accommodating a control circuit 16. The control circuit 16 is electrically connected to the first light source 141 and the second light source 142 in the illumination assembly 14, respectively, and is used to control the on / off state and luminous intensity of the first light source 141 and the second light source 142 in each illumination assembly 14. In at least one embodiment of the present application, the electrical connection between the control circuit 16 and the first light source 141 and the second light source 142 can be achieved by embedding wiring (not shown) in the housing 11.

[0071] In this embodiment, the photosynthetic organism cultivation device 100 further includes a first adjustment knob 171 and a second adjustment knob 172. The first adjustment knob 171 and the second adjustment knob 172 are movably connected to the third surface 113 of the housing 11 and are electrically connected to the control circuit 16. By rotating the first adjustment knob 171 and the second adjustment knob 172, the luminous intensity of the first light source 141 and the second light source 142 can be controlled, respectively.

[0072] By controlling the on / off states and luminous intensities of the first and second light sources 141, 142 in each illumination assembly 14 through the control circuit 16, the first adjustment knob 171, and the second adjustment knob 172, illumination with different light qualities can be adjusted to illuminate the photosynthetic organisms within the culture dishes 13. Furthermore, in this embodiment, the housing 11 is made of a light-shielding material, and the multiple culture dishes 13 are stacked, so that the illumination light emitted by the illumination assembly 14 in each cultivation unit is isolated from each other, which helps prevent crosstalk between the illumination light in each cultivation unit. That is, in this embodiment, the first and second light sources 141, 142 in each cultivation unit can be independently controlled to provide illumination with different light qualities to each culture dish 13. This facilitates simultaneous observation of the growth of photosynthetic organisms under different lighting conditions.

[0073] In natural environments, photosynthetic organisms such as plants and microalgae receive a full spectrum of sunlight. However, in artificial lighting conditions, such as greenhouses or indoor cultivation, specific wavelengths of light are often used to optimize the growth of photosynthetic organisms. For example, LED plant growth lights can adjust the intensity of different wavelengths of light to simulate natural sunlight or optimize the growth conditions of specific photosynthetic organisms.

[0074] The photosynthetic organism cultivation device 100 of the present application embodiment can be used for the early stage cultivation condition screening under the laboratory scene, and can also be used for the screening of mutant strains. Photosynthetic organisms mainly use the light in the visible spectrum (approximately between 380 and 700 nanometers) to photosynthesize. However, some photosynthetic organisms can use the ultraviolet (UV-A and UV-B) or infrared (near infrared) part of the spectrum. For these organisms, their pigment complexes or pigment-binding proteins may have different spectral absorption characteristics, enabling them to utilize light energy outside the visible spectrum. By combining ultraviolet or infrared light, it can be used to screen mutants of this type for subsequent synthetic biology chassis cells.

[0075] Therefore, the photosynthetic organism cultivation device 100 of the embodiment of the present application can control the switching and luminous intensity of the first light source 141 and the second light source 142 in each lighting component 14 by the control circuit 50, so that each culture dish 13 can receive illumination light of different light qualities, thereby simulating various lighting conditions, observing the growth of photosynthetic organisms in the culture dish 13 under various lighting conditions, and thus screening out the better lighting conditions required for a specific variety of photosynthetic organisms. In addition, by adjusting the ratio of different light qualities, microbial strains that are adapted to various culture conditions can be screened out to adapt to a specific lighting environment or to conduct research on the mechanism of photosynthetic pathways under different light qualities. Furthermore, the photosynthetic organism cultivation device 100 can allocate illumination light of different light qualities for different varieties of photosynthetic organisms, so that the photosynthetic organism cultivation device 100 can be applicable to the cultivation process of multiple different varieties of photosynthetic organisms.

[0076] Furthermore, the present application adopts a magnetic attraction method to connect two adjacent cultivation units 10. The culture dish 13 can be conveniently taken in and out by simply overcoming the magnetic force and taking the shell 11.

[0077] Those skilled in the art should recognize that the above embodiments are merely intended to illustrate the present application and are not intended to limit the present application. As long as they are within the spirit of the present application, appropriate changes and modifications to the above embodiments are within the scope of protection claimed in the present application.

Claims

1. A photosynthetic organism cultivation device, characterized in that: It comprises at least two cultivation units arranged in a stacked manner, wherein the at least two cultivation units are magnetically connected in sequence; Each of the cultivation units comprises: A housing is formed with a mounting hole, and the housing is made of a light-shielding material; a culture dish, located in the mounting hole, for accommodating photosynthetic organisms; an illumination assembly, located in the mounting hole, comprising at least a first light source and a second light source, wherein the first light source and the second light source are configured to emit illumination light of different wavelengths; a light guide assembly, located in the mounting hole and on the optical path of the illumination light, for diffusing the illumination light and guiding the diffused illumination light to the culture dish; and The control circuit is electrically connected to the first light source and the second light source, and is used to control the switching state and luminous intensity of the first light source and the second light source.

2. The photosynthetic organism cultivation device according to claim 1, characterized in that: Each of the cultivation units further comprises a magnetic attraction structure, which is fixed to the shell and is used for magnetic attraction with the magnetic attraction structure in an adjacently arranged cultivation unit, so that the at least two cultivation units are connected.

3. The photosynthetic organism cultivation device according to claim 2, characterized in that: The housing comprises a first surface and a second surface parallel to each other, and the mounting hole passes through the first surface and the second surface; The photosynthetic organism cultivation device includes two magnetic structures, one of which is exposed relative to the first surface, and the other is exposed relative to the second surface.

4. The photosynthetic organism cultivation device according to claim 2, characterized in that: The magnetic attraction structure includes a first contact terminal and a second contact terminal that are spaced apart. One of the first contact terminal and the second contact terminal serves as a positive electrode, and the other serves as a negative electrode, and both are electrically connected to the control circuit.

5. The photosynthetic organism cultivation device according to claim 4, characterized in that: The first contact terminal surrounds the second contact terminal.

6. The photosynthetic organism cultivation device according to claim 2, characterized in that: The housing further includes a light shielding plate, the light shielding plate covering the opening of the mounting hole on the first surface; The lighting component and the light guide component are located between the light shielding plate and the culture dish.

7. The photosynthetic organism cultivation device according to any one of claims 1 to 6, characterized in that: The light guide assembly includes a reflective sheet, a light guide plate, and a diffuser that are stacked in sequence, and the diffuser is closer to the culture dish than the reflective sheet; The reflective sheet is used to reflect the received illumination light, and the diffusion sheet is used to diffuse the illumination light from the light guide plate.

8. The photosynthetic organism cultivation device according to claim 7, characterized in that: The light guide plate has a circular planar structure, and the first light source and the second light source are spaced and symmetrically arranged along the circumference of the light guide plate.

9. The photosynthetic organism cultivation device according to claim 8, characterized in that: The light emitting surfaces of the first light source and the second light source are attached to the light guide plate.

10. The photosynthetic organism cultivation device according to any one of claims 1 to 5, characterized in that: Each of the cultivation units further comprises a first adjusting knob and a second adjusting knob movably connected to the shell. By rotating the first adjusting knob and the second adjusting knob, the luminous intensity of the first light source and the second light source can be adjusted respectively.

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