Laboratory brain slice patch device

Abstract

The utility model discloses a laboratory brain slice patching device, relates to medical technical field, including box body, cover plate, slide holder, press shaft and taking device, the slide holder is provided in box body and is used for fixing glass slide, box body side is provided with chute, is provided with press shaft that can slide along chute in the chute, cover plate can cover on the box body, and the cover plate can cover on the box body. And the cover plate is provided with a piece taking device for fixing the cover glass. According to the utility model, the slide holder is used for bearing a glass slide, the slide taking device is used for fixing the cover glass, the cover glass is attached to the glass slide through the covering process of the cover plate and the box body, then the button is pressed to enable the pressing shaft to slide left and right relative to the sliding chute on the box body to press the cover glass and the glass slide, and residual bubbles are discharged; the problem that bubbles are likely to occur in the process of placing the cover glass in a patch mode is solved.

Description

Technical Field

[0001] The utility model relates to the field of medical technology, in particular to a laboratory brain slice patch device. Background Art

[0002] Frozen sections are the most widely used method in conventional histological preparation techniques. They are not only used to observe the morphology and structure of normal cells and tissues, but are also a primary method for studying, observing, and assessing morphological changes in cells and tissues in clinical, research pathology, and forensic medicine. They are also widely used in research across many other disciplines. In scientific experiments, clinical medicine, and experimental teaching, most specimens prepared for light microscopic observation are prepared using frozen sections. Living cells and tissues are generally colorless and transparent, lacking contrast between tissues and within cells, making them difficult to distinguish clearly under a standard light microscope. Once outside the body, tissues quickly die and decay, losing their normal structure. Therefore, tissues undergo a series of steps, including fixation with a medium, frozen sectioning, removal of the medium, and staining, to prevent cell and tissue death and ensure clear identification of their morphology and structure. The frozen section preparation process includes sampling, fixation, washing and dehydration, addition of a medium, frozen sectioning, retrieval, spreading, mounting, staining, dehydration, clearing, and mounting.

[0003] However, during the patching step, bubbles are likely to appear between the coverslip and the slide during the placement of the coverslip. These bubbles interfere with the experimenter's observation of the slices, and thus meticulous bubble elimination is necessary after patching, which seriously restricts the patching speed. Based on this, a laboratory brain slice patching device is proposed. Utility Model Content

[0004] The utility model aims to provide a laboratory brain slice patch device to solve the problem that bubbles are easily generated during the process of placing the patch on a cover glass.

[0005] In order to achieve the above-mentioned purpose, the present invention provides the following technical solutions:

[0006] A laboratory brain slice patch device includes a box body, a cover plate, a slide stage, a pressure shaft and a slide taking device. The box body is provided with a accommodating space with an upward opening. The slide stage is arranged in the box body and is used to fix the slide. A slide groove is provided on the side of the box body. A pressure shaft that can slide along the slide groove is provided in the slide groove. The pressure shaft is placed above the slide stage. The cover plate is hinged to the box body and can be covered on the box body. The cover plate is provided with a slide taking device for fixing the cover slide.

[0007] Furthermore, the wafer stage is provided with edges and grooves around it.

[0008] Furthermore, the slide groove is provided with a convex point.

[0009] Furthermore, buttons are provided on both sides of the pressing shaft to facilitate sliding the pressing shaft.

[0010] Furthermore, the film taking device is a suction cup, and the suction cup is connected to the cover plate through a flexible shaft.

[0011] The principle and beneficial effects of this technical solution:

[0012] The utility model supports the slide glass by the slide stage, fixes the cover glass by the slide taking device, and makes the cover glass and the slide glass fit together by the covering process of the cover plate and the box body. Since the box body and the cover plate are hinged on one side, the cover glass must first contact the slide glass on one side, and then the cover glass slowly descends to fit the slide glass, so that the air is discharged from the other side, thereby reducing or eliminating the generation of bubbles. Then, the button is pressed to make the pressing shaft slide left and right relative to the slide groove on the box body to press the cover glass and the slide glass, thereby discharging the remaining bubbles. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 This is a schematic diagram of a laboratory brain slice patch device;

[0014] Figure 2 A laboratory brain slice patch device Figure 1 A magnified schematic diagram of point A;

[0015] Figure 3 A cross-sectional view of a laboratory brain slice patch device;

[0016] Figure 4 A top view of the box of a laboratory brain slice patch device.

[0017] The reference numerals in the drawings of the specification include:

[0018] 1. Box body; 2. Cover plate; 3. Slide stage; 4. Press shaft; 5. Slide removal device; 6. Slide groove; 7. Suction cup; 8. Flexible shaft; 9. Clamping edge; 10. Groove; 11. Bump; 12. Button; 13. Cover glass; 14. Slide. DETAILED DESCRIPTION

[0019] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments:

[0020] like Figure 1-4The figure shows a laboratory brain slice mounting device, comprising a box body 1, a cover plate 2, a slide stage 3, a pressure shaft 4, and a slide removal device 5. The box body 1 has an upwardly opening storage space. The slide stage 3 is disposed within the box body 1 and is used to secure a glass slide 14. The slide stage 3 is surrounded by retaining edges 9 and grooves 10 to facilitate securing and removing the glass slide 14. A chute 6 is provided on the side of the box body 1. A pressure shaft 4 is disposed within the chute 6 and can slide along the chute 6. The pressure shaft 4 is positioned above the slide stage 3. The chute 6 is provided with a bump 11 that allows the pressure shaft 4 to temporarily rest in a certain position within the chute 6 without preventing the pressure shaft 4 from continuing to slide. The cover plate 2 is hinged to the box body 1, and the cover plate 2 can be covered on the box body 1. A film-taking device 5 is provided on the cover plate 2 for fixing the cover glass 13. The film-taking device 5 is a suction cup 7, which can suck the back of the cover glass 13. The suction cup 7 is connected to the cover plate 2 through a soft shaft 8. The soft shaft 8 allows the cover glass 13 sucked by the suction cup 7 to have a certain amount of movement space, making it easier to fit with the slide 14.

[0021] As an embodiment of the present invention, buttons 12 are provided on both sides of the pressing shaft 4 for facilitating the sliding of the pressing shaft 4 .

[0022] The specific implementation process is as follows:

[0023] First, spread the frozen brain slices on the slide 14, place the slide 14 on the slide table 3, then adsorb the back of the cover glass 13 on the suction cup 7, slowly cover the cover plate 2 on the box body 1, so that the cover glass 13 fixed by the suction cup 7 fits with the slide 14 on the slide table 3. Since the box body 1 and the cover plate 2 are hinged on one side, the cover glass 13 must be in contact with the slide 14 on one side first, and then the cover glass 13 is slowly lowered to fit the slide 14, so that the space is large. The air is discharged from the other side to reduce or eliminate the generation of bubbles. Then, press the buttons 12 on both sides of the pressing shaft 4 to slide the pressing shaft 4 on the slide groove 6 until it contacts the convex point 11 on the slide groove 6. The position of the convex point 11 can enable the pressing shaft 4 to press a part of the cover glass 13. Then, open the cover plate 2, and press the pressing shaft 4 to press the cover glass 13 to separate the cover glass 13 from the suction cup 7. Finally, slide the pressing shaft 4 left and right to discharge the remaining bubbles between the cover glass 13 and the slide glass 14.

[0024] The above description is merely an embodiment of the present invention, and the commonly known specific technical solutions and / or features of the solution are not described in detail here. It should be noted that for those skilled in the art, without departing from the technical solution of the present invention, several variations and improvements can be made, which should also be considered as the scope of protection of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection claimed in this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A laboratory brain slice patch device, characterized by: The invention comprises a box body (1), a cover plate (2), a slide table (3), a pressure shaft (4) and a film taking device (5); the box body (1) is provided with a accommodating space with an opening facing upward; the slide table (3) is provided in the box body (1) and is used to fix the slide (14); a slide groove (6) is provided on the side of the box body (1); a pressure shaft (4) capable of sliding along the slide groove (6) is provided in the slide groove (6); the pressure shaft (4) is placed above the slide table (3); the cover plate (2) and the box body (1) are in a hinged relationship; the cover plate (2) can be covered on the box body (1); and the cover plate (2) is provided with a film taking device (5) for fixing the cover glass (13).

2. A laboratory brain slice patch device according to claim 1, characterized in that: The wafer carrier (3) is provided with edges (9) and grooves (10) around its periphery.

3. A laboratory brain slice patch device according to claim 1, characterized in that: The slide groove (6) is provided with a convex point (11).

4. The laboratory brain slice patch device according to claim 1, characterized in that: Buttons (12) are provided on both sides of the pressing shaft (4) for facilitating the sliding of the pressing shaft (4).

5. The laboratory brain slice patch device according to claim 1, characterized in that: The film taking device (5) is a suction cup (7), and the suction cup (7) is connected to the cover plate (2) via a flexible shaft (8).

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