Protein purification chromatography device

Abstract

The utility model provides a protein purification chromatography device which comprises a chromatography box, a base, an auxiliary discharging assembly and a discharging pipeline, the base is welded at the bottom of the chromatography box, a partition plate is welded in the chromatography box, the whole chromatography box is in an inclined state, the auxiliary discharging assembly is inserted in the top end of the chromatography box in a penetrating mode, and the discharging pipeline is connected with the auxiliary discharging assembly in a penetrating mode. A discharging pipeline is inserted into the bottom of the side edge of the chromatography box, and the tail end of each auxiliary discharging assembly is attached to the side edge of the partition plate. According to the protein purification chromatography device, the chromatography box in an inclined state is adopted, and the bottom purification layer and the top purification layer are arranged in the chromatography box, so that after raw materials are injected, each purification layer can be automatically and sequentially filled from bottom to top, and a protein solution obtained through chromatography can be accumulated at the bottom in the filling process, the purification efficiency is higher, and the purification effect is better. The discharge efficiency of the protein solution is improved, and the phenomenon that the top solution after chromatography flows downwards along with the protein solution at the bottom layer is effectively relieved.

Description

Technical Field

[0001] The utility model relates to the technical field of protein purification, in particular to a protein purification chromatography device. Background Art

[0002] Protein purification chromatography can separate and purify specific proteins from complex biological samples to increase their purity and concentration for subsequent biological research or applications. According to prior art, such as the column chromatography device for monoclonal antibody protein purification described in Chinese patent document CN212357110U, the disclosed technical solution involves substances that pass through the first dialysis layer entering the second liquid inlet pipe. Substances that cannot pass through the first dialysis layer gradually accumulate and are then discharged through the first liquid discharge pipe, thereby maintaining the concentration of non-dialyzed substances within the first chromatographic column within a certain range. Similarly, substances that enter the second chromatographic column through the second liquid inlet pipe repeat the above steps. Substances that pass through the second dialysis layer ultimately enter an external protein detection device for detection.

[0003] According to the disclosed technical solution, the prior art protein purification chromatography device sequentially guides the protein solution through multi-layer volumetric flasks. Therefore, the equipment occupies a large space, is inconvenient to carry, and is inefficient. It is also difficult to quickly and efficiently discharge the protein liquid components obtained by chromatography. Utility Model Content

[0004] In view of the shortcomings of the existing technology, the purpose of the present invention is to provide a protein purification chromatography device to solve the problems raised in the above background technology. The present invention improves space utilization, increases the efficiency of purification chromatography, and makes the discharge process more efficient and thorough.

[0005] In order to achieve the above-mentioned purpose, the present invention is implemented through the following technical solutions: a protein purification chromatography device, comprising a purification chromatography device body, the purification chromatography device body comprising a chromatography box, a base, an auxiliary discharge component and a discharge pipe, the bottom of the chromatography box is welded with a base, the interior of the chromatography box is welded with a partition, and the chromatography box as a whole is in an inclined state, the top of the chromatography box is inserted with an auxiliary discharge component, and the side bottom of the chromatography box is plugged with a discharge pipe, and the end of each auxiliary discharge component is in contact with the side of the partition.

[0006] Furthermore, the interior of the chromatography box is divided into a bottom purification layer and a top purification layer, a side of the bottom purification layer is connected to an injection pipe, and the bottom purification layer and the top purification layer are separated by a partition.

[0007] Furthermore, the top of each partition is on the same vertical plane, and the tops of the bottom purification layer and the top purification layer are connected to each other, and the sides of the discharge pipe are respectively connected to the bottom ends of the bottom purification layer and the top purification layer.

[0008] Furthermore, the discharge pipe includes a discharge pipe and a collecting pipe, the end of the discharge pipe is welded with a collecting pipe, the bottom ends of the bottom purification layer and the top purification layer are provided with discharge ports, and the discharge pipe and the discharge ports are connected.

[0009] Furthermore, a threaded sleeve is provided at the top of the collecting pipe, a second threaded column is inserted into the interior of the threaded sleeve, a sealing sleeve is welded to the end of the second threaded column, and the side of the sealing sleeve is in contact with the inner wall of the collecting pipe.

[0010] Furthermore, the auxiliary discharge assembly includes a first threaded column, a top bonding plate and a bottom bonding plate, and the top end of the first threaded column is integrally formed with a hand wheel.

[0011] Furthermore, the end of the first threaded column is provided with a docking bearing, and the docking bearing is integrally embedded in the middle position of the surface of the top bonding plate. The surfaces of the top bonding plate and the bottom bonding plate are both provided with through holes, and the surface of the top bonding plate is also provided with a guide hole.

[0012] Furthermore, a guide rod is welded on the surface of the bottom bonding plate, and the guide rod passes upward from the inside of the guide hole, and the positions of the through holes opened on the top bonding plate and the bottom bonding plate are staggered with each other.

[0013] Beneficial effects of the utility model:

[0014] 1. The protein purification chromatography device adopts an inclined chromatography box with a bottom purification layer and a top purification layer arranged inside. After the raw material is injected, each purification layer is automatically filled from bottom to top in sequence. During the filling process, the chromatographic protein solution can be accumulated at the bottom, which improves the purification efficiency.

[0015] 2. The protein purification chromatography device controls the auxiliary discharge component to push along the purification layer. After the chromatography is completed, the thrust is used to increase the discharge pressure of the chromatographic protein solution, thereby improving the discharge efficiency of the protein solution without interfering with the process of raw material injection and upward spread chromatography.

[0016] 3. The protein purification chromatography device is connected to a discharge pipe on the side of the chromatography box. With the help of this discharge pipe and the auxiliary discharge component, when discharging the protein solution after chromatography, the protein solution at the bottom of the purification layer can be discharged from bottom to top, effectively reducing the phenomenon that the top solution after chromatography flows downward with the protein solution at the bottom layer. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic structural diagram of the appearance of a protein purification chromatography device of the present invention;

[0018] Figure 2 This is a side sectional view of a protein purification chromatography device of the present invention;

[0019] Figure 3 This is a disassembled diagram of the auxiliary discharge component of the utility model;

[0020] Figure 4 This is a disassembled diagram of the discharge pipe part of the utility model;

[0021] In the figure: 1. Chromatography box; 2. Base; 3. Injection pipe; 4. Auxiliary discharge assembly; 5. Discharge pipe; 6. Bottom purification layer; 7. Partition; 8. Top purification layer; 9. Discharge port; 10. First threaded column; 11. Handwheel; 12. Docking bearing; 13. Top fitting plate; 14. Bottom fitting plate; 15. Through hole; 16. Guide rod; 17. Guide hole; 18. Discharge pipe; 19. Collecting pipe; 20. Blocking sleeve; 21. Second threaded column; 22. Threaded sleeve. DETAILED DESCRIPTION

[0022] In order to make the technical means, creative features, objectives and effects achieved by the present invention easier to understand, the present invention is further described below in conjunction with specific implementation methods.

[0023] See also Figures 1 to 4 The present invention provides the following technical solution: a protein purification chromatography device, comprising a purification chromatography device body, the purification chromatography device body comprising a chromatography box 1, a base 2, an auxiliary discharge assembly 4, and a discharge pipe 5. The base 2 is welded to the bottom of the chromatography box 1, a partition 7 is welded inside the chromatography box 1, and the chromatography box 1 is tilted as a whole. The auxiliary discharge assembly 4 is inserted through the top of the chromatography box 1, and the discharge pipe 5 is inserted into the bottom side of the chromatography box 1. The end of each auxiliary discharge assembly 4 is in contact with the side of the partition 7. The protein purification chromatography device is used to purify the injected protein raw material solution by chromatography.

[0024] When the present invention is used, the raw material solution is injected from the injection pipe 3 into the middle position of the bottom purification layer 6, so that the raw material can first fill the bottom purification layer 6. During this process, chromatography treatment can be performed. After the bottom purification layer 6 is filled, only the solution at the top layer will flow into the first top purification layer 8 above, and the raw material that has been chromatographed and purified once will be purified again in this cavity. The above process is repeated until each purification layer is filled. The bottom solution of the chromatography liquid can be discharged. During the discharge, the auxiliary discharge component 4 is manually controlled to push the solution in each purification layer toward the bottom at a time, so as to accelerate the chromatographic protein solution into the discharge pipe 5.

[0025] In this embodiment, the interior of the chromatography chamber 1 is divided into a bottom purification layer 6 and a top purification layer 8. The bottom purification layer 6 is connected to an injection pipe 3 on its side, and the bottom and top purification layers 6, 8 are separated by a partition 7. The top of each partition 7 is located on the same vertical plane, and the tops of the bottom and top purification layers 6, 8 are interconnected. The side of the discharge pipe 5 is connected to the bottom of the bottom and top purification layers 6, 8, respectively. By using an inclined chromatography chamber 1 with the bottom and top purification layers 6, 8 inside, each purification layer can be automatically filled from bottom to top after the raw material is injected. During the filling process, the chromatographic protein solution is accumulated at the bottom, resulting in higher purification efficiency.

[0026] Specifically, since the bottom purification layer 6 and the top purification layer 8 are separated by the partition 7 and the top height of each partition 7 is increased, after the bottom purification layer 6 is filled with solution, only the surface solution can flow into the second top purification layer 8. Through this process, the precipitated protein solution portion at the bottom can be retained, thereby achieving the effect of multiple chromatography.

[0027] In this embodiment, the discharge conduit 5 includes a discharge conduit 18 and a collection conduit 19. The collection conduit 19 is welded to the end of the discharge conduit 18. A discharge port 9 is defined on the sides of the bottom ends of the bottom purification layer 6 and the top purification layer 8, and the discharge conduit 18 and the discharge port 9 are connected. A threaded sleeve 22 is defined at the top of the collection conduit 19. A second threaded column 21 is inserted into the threaded sleeve 22. A sealing sleeve 20 is welded to the end of the second threaded column 21. The side of the sealing sleeve 20 is aligned with the inner wall of the collection conduit 19. The discharge conduit 5 is connected to the side of the chromatography chamber 1. By combining this discharge conduit 5 with the auxiliary discharge assembly 4, the protein solution at the bottom of the purification layer can be discharged sequentially from bottom to top during the discharge of the chromatographic protein solution. This effectively reduces the phenomenon of the top solution flowing downward with the protein solution at the bottom layer after chromatography.

[0028] Specifically, the auxiliary discharge assembly 4 pushes the solution to partially push the bottom protein solution in each purification layer into the discharge pipe 18. At this time, by rotating the second threaded column 21, the sealing sleeve 20 is controlled to first seal the two top discharge pipes 18, leaving only the bottom discharge pipe 18, so that the protein solution in the bottom purification layer 6 can be discharged first. After the protein solution in the bottom purification layer 6 is discharged, the external container is changed to discharge the upper layer solution of the bottom purification layer 6 into another container until the bottom purification layer 6 is completely discharged. Then, the second threaded column 21 can be rotated again, and the above process can be repeated to discharge the solutions in the two subsequent top purification layers 8 step by step.

[0029] In this embodiment, the auxiliary discharge assembly 4 includes a first threaded column 10, a top bonding plate 13, and a bottom bonding plate 14. The top of the first threaded column 10 is integrally formed with a handwheel 11. The end of the first threaded column 10 is sleeved with a docking bearing 12, and the docking bearing 12 is integrally embedded in the middle position of the surface of the top bonding plate 13. The surfaces of the top bonding plate 13 and the bottom bonding plate 14 are both provided with through holes 15, and the surface of the top bonding plate 13 is also provided with a guide hole 17. A guide rod 16 is welded to the surface of the bottom bonding plate 14, and the guide rod 16 passes upward from the inside of the guide hole 17. The through holes 15 provided on the top bonding plate 13 and the bottom bonding plate 14 are staggered. By controlling the auxiliary discharge component 4 to push along the purification layer, the thrust can be used to increase the discharge pressure of the protein solution after chromatography, thereby improving the discharge efficiency of the protein solution without interfering with the process of the raw material injection and upward spread chromatography. By turning the top handwheel 11, the top bonding plate 13 is pushed downward by the rotation of the first threaded column 10, and the side of the bottom bonding plate 14 is squeezed and clamped by the purification layer and will not move downward until the first bonding plate and the second bonding plate are bonded. At this time, each through hole 15 on the two bonding plates can be staggered. Continuing to turn the handwheel 11, the two bonding plates can be pushed downward to squeeze the solution at the same time, thereby achieving the effect of accelerating the discharge of the internal solution.

[0030] The above shows and describes the basic principles, main features and advantages of the present invention. For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention.

[0031] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

Claims

1. A protein purification chromatography device, comprising a purification chromatography device body, characterized in that: The purification chromatography device body comprises a chromatography box (1), a base (2), an auxiliary discharge assembly (4) and a discharge pipe (5); the base (2) is welded to the bottom of the chromatography box (1); a partition (7) is welded inside the chromatography box (1); and the chromatography box (1) is tilted as a whole; the auxiliary discharge assembly (4) is inserted through the top of the chromatography box (1); the discharge pipe (5) is plugged into the bottom side of the chromatography box (1); and the end of each auxiliary discharge assembly (4) is in contact with the side of the partition (7).

2. A protein purification chromatography device according to claim 1, characterized in that: The interior of the chromatography box (1) is divided into a bottom purification layer (6) and a top purification layer (8), the side of the bottom purification layer (6) is connected to an injection pipe (3), and the bottom purification layer (6) and the top purification layer (8) are separated by a partition (7).

3. A protein purification chromatography device according to claim 2, characterized in that: The top of each partition (7) is located on the same vertical plane, and the tops of the bottom purification layer (6) and the top purification layer (8) are connected to each other, and the sides of the discharge pipe (5) are respectively connected to the bottom ends of the bottom purification layer (6) and the top purification layer (8).

4. A protein purification chromatography device according to claim 2, characterized in that: The discharge pipe (5) comprises a discharge pipe (18) and a collecting pipe (19), the collecting pipe (19) is welded to the end of the discharge pipe (18), and the bottom ends of the bottom purification layer (6) and the top purification layer (8) are provided with a discharge port (9), and the discharge pipe (18) and the discharge port (9) are connected.

5. A protein purification chromatography device according to claim 4, characterized in that: A threaded sleeve (22) is provided at the top end of the collecting pipe (19), a second threaded column (21) is inserted into the interior of the threaded sleeve (22), a sealing sleeve (20) is welded to the end of the second threaded column (21), and the side of the sealing sleeve (20) is in contact with the inner wall of the collecting pipe (19).

6. A protein purification chromatography device according to claim 2, characterized in that: The auxiliary discharge assembly (4) comprises a first threaded column (10), a top laminating plate (13) and a bottom laminating plate (14); the top end of the first threaded column (10) is integrally formed with a hand wheel (11).

7. A protein purification chromatography device according to claim 6, characterized in that: The end of the first threaded column (10) is sleeved with a docking bearing (12), and the docking bearing (12) is integrally embedded in the middle position of the surface of the top bonding plate (13). The surfaces of the top bonding plate (13) and the bottom bonding plate (14) are both provided with through holes (15), and the surface of the top bonding plate (13) is also provided with a guide hole (17).

8. A protein purification chromatography device according to claim 7, characterized in that: A guide rod (16) is welded on the surface of the bottom bonding plate (14), and the guide rod (16) passes upward from the inside of the guide hole (17), and the positions of the through holes (15) opened on the top bonding plate (13) and the bottom bonding plate (14) are staggered.

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