Sperm penetrating power detection device

Abstract

The utility model relates to a sperm penetrating power detection device which comprises a glass cover plate and a glass base, a whitening area and a detection area are arranged on the glass base, the glass cover plate covers the detection area, the detection area is uniformly divided into a plurality of independent detection chambers, and a sample adding groove and a sperm storage pool which are connected with each other are arranged in each detection chamber. The sperm penetrating power detection device disclosed by the utility model can accurately detect the sperm penetrating power, and can better evaluate the contraception or pregnancy assisting effect of gel.

Description

Technical Field

[0001] The utility model relates to the field of reproductive medicine, in particular to a sperm penetration ability detection device. Background Art

[0002] In recent years, researchers have developed a variety of topical gels for the female reproductive tract, including contraceptive gels, fertility-aiding gels, and anti-genital tract infection gels.

[0003] Contraceptive gel is a hydrophilic gel with excellent acid-buffering properties that is placed in the female vagina. It quickly forms a semisolid polymer protective film on the surface of the cervix and vaginal mucosa, providing contraception and preventing sexually transmitted diseases similar to traditional condoms. Contraceptive gel acts as a physical barrier within the reproductive tract to prevent sperm from reaching the uterus; it can also act as a chemical barrier, containing pharmaceutical preparations that inactivate sperm in the vagina; or it can combine physical and chemical barriers to prevent sperm from meeting the egg, achieving contraception. Once placed in the vagina, the contraceptive gel forms a semisolid polymer protective film on the vaginal wall, acting as a physical barrier. When in contact with semen, it increases its viscosity, reduces sperm motility, and prevents sperm from entering the uterus. Contraceptive gel is suitable for short-term vaginal contraception and sometimes also has certain lubricating and antibacterial properties.

[0004] Fertility gel is a hydrophilic gel with excellent acid-neutralizing properties that is placed in the female vagina. It forms a liquid polymer copolymer neutralizing membrane on the surface of the vaginal mucosa, temporarily neutralizing the acidic environment within the vagina and preventing sperm from rapidly inactivating due to the low pH environment. The medication contained in the fertility gel promotes sperm capacitation within the vagina, allowing sperm to pass smoothly through the vagina into the uterus until they meet and combine with the egg in the fallopian tube, thereby achieving the purpose of assisting pregnancy. Fertility gel for the female reproductive tract is suitable for short-term vaginal pregnancy assistance. It can neutralize the acidic environment in the vagina in a short period of time, while also providing a certain degree of lubrication and promoting sperm capacitation.

[0005] Therefore, detecting the ability of sperm to penetrate the gel is one of the important indicators for evaluating the contraceptive effect of external contraceptive gels and the assisted pregnancy effect of external assisted pregnancy gels. Utility Model Content

[0006] In order to solve the technical problems existing in the prior art, the utility model provides a sperm penetration ability detection device.

[0007] In order to achieve the above object, the technical solution of the utility model is as follows:

[0008] A sperm penetration ability detection device includes a glass cover and a glass base. The glass base is provided with a whitened area and a detection area. The glass cover covers the detection area. The detection area is evenly divided into several independent detection chambers. The detection chambers are provided with interconnected sample loading grooves and sperm storage tanks.

[0009] As a preferred technical solution, the glass cover is 50-80 mm long, 20-25 mm wide, and 0.15-0.2 mm thick, and there is a gap of 0.01-0.1 mm between the glass cover and the glass base.

[0010] As a preferred technical solution, the detection area is evenly divided into 3-5 independent detection chambers by epoxy resin.

[0011] As an optimal technical solution, the detection chamber is 15 to 20 mm wide, with openings at both ends. A sample loading slot is provided in the middle of the detection chamber, and a sperm storage pool is engraved in the middle of the front end of the detection chamber. The front end of the sample loading slot is connected to the sperm storage pool, and the gel sample is filled in the sample loading slot.

[0012] As an optimal technical solution, the sperm storage pool is a 1 / 4 spherical groove with a spherical radius of 0.7-1.2 mm. The sample adding groove is 15-20 mm long and 0.2-0.4 mm deep. The sample adding groove is engraved on the glass base.

[0013] As a preferred technical solution, scale bars are provided on both sides of the sample adding groove, the scale bars are 2 to 4 mm away from the sample adding groove, the scale bars are 20 to 25 mm long, and the minimum scale is 0.1 mm.

[0014] As a preferred technical solution, a partition strip is provided on the edge of the detection chamber.

[0015] Compared with the prior art, the beneficial effects of the present invention are:

[0016] The sperm penetration ability detection device of the utility model can accurately detect the sperm penetration ability and can better evaluate the contraceptive or pregnancy-aiding effect of the gel. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic structural diagram of a sperm penetration ability detection device of the present invention;

[0018] Figure 2 This is a structural schematic diagram of a glass cover in the sperm penetration ability detection device of the present invention;

[0019] Figure 3 It is a structural schematic diagram of a glass base in a sperm penetration ability detection device of the present invention.

[0020] In the figure: 1. White-painted area; 2. Detection area; 3. Detection chamber; 4. Sperm reservoir; 5. Sample loading slot; 6. Scale bar; 7. Divider bar; 8. Glass cover slip. DETAILED DESCRIPTION

[0021] The technical solution of the present invention is further described below in conjunction with specific embodiments:

[0022] like Figure 1 As shown, a sperm penetration ability detection device includes a glass cover 8 and a glass base. The glass base is provided with a white area 1 and a detection area 2. The white area 1 is used for writing labels; the detection area 2 is used for sample detection. The glass cover 8 covers the detection area 2.

[0023] like Figure 2 As shown, the glass cover 8 is 50-80 mm long, 20-25 mm wide, and 0.15-0.2 mm thick, and there is a gap of 0.01-0.1 mm between the glass cover 8 and the glass base.

[0024] The detection area 2 is evenly divided into 3-5 independent detection chambers 3 by epoxy resin.

[0025] like Figure 3 As shown, the detection chamber 3 is 15 to 20 mm wide, with openings at both the front and rear ends. A sample loading groove 5 is provided in the middle of the detection chamber 3, and a sperm storage pool 4 is engraved in the middle of the front end of the detection chamber 3. The front end of the sample loading groove 5 is connected to the sperm storage pool 4, and the sample loading groove 5 is convenient for adding gel samples with higher viscosity.

[0026] The sperm storage pool 4 is a 1 / 4 spherical groove with a spherical radius of 0.7-1.2 mm. The sample adding groove 5 is 15-20 mm long and 0.2-0.4 mm deep. The sample adding groove 5 is engraved on the glass base.

[0027] Scale bars 6 are provided on both sides of the sample adding groove 5 . The scale bars 6 are 2 to 4 mm away from the sample adding groove 5 . The scale bars 6 are 20 to 25 mm long, and the minimum scale is 0.1 mm.

[0028] A separation strip 7 is provided at the edge of the detection chamber 3 for adhering a glass cover 8 and separating the detection chambers 3 .

[0029] Example 1

[0030] In this embodiment, the method for using the sperm penetration ability detection device includes the following steps:

[0031] S1. Take liquefied semen and perform upstream test to obtain high-motility sperm, then adjust the sperm concentration to 1×10 7 / ml, as a backup;

[0032] S2. The gel sample to be tested is added to the sample loading tank 5 from the front end of the detection chamber 3. Under the action of siphon, the detection chamber 3 is filled with the gel sample to be tested;

[0033] S3. Use vaseline or other sperm-safe sealant to seal the rear opening of the detection chamber 3;

[0034] S4. Add 3 to 5 μl of sperm from step S1 to the sperm reservoir 4 in the detection chamber 3;

[0035] S5. Place the sperm penetration test device in a humidified chamber and incubate at 37°C in a 5% CO2 incubator for 10-30 min.

[0036] S6. Observe under a microscope and record the presence of sperm, sperm count, and maximum distance of sperm movement in chamber 3;

[0037] S7. Compare the data recorded in step S6 with the control group to determine the barrier effect of the gel on sperm. The control group uses the same sperm penetration ability testing device as above, except that the gel is replaced with sperm culture medium (containing 1% bovine serum albumin)

[0038] According to the method of using the sperm penetration ability detection device described above, a more universal sperm penetration ability detection method can be obtained, which comprises the following steps:

[0039] Step 1: Determine the swimming direction of sperm;

[0040] This step can be achieved by the detection chamber 3 in the above device, and the swimming direction of the sperm is determined to be along the length direction of the sample loading groove 5.

[0041] Step 2: placing gel in the direction of sperm swimming;

[0042] This step can be achieved by adding the gel sample to be tested and filling the detection chamber 3 .

[0043] Step three: testing the penetration of sperm into the gel to determine the sperm's ability to penetrate the gel.

[0044] This step can be accomplished by observing the position of sperm within the gel under a microscope. Specifically, the sperm penetration test uses a scale to measure the distance the sperm travel within the gel, and then determines the sperm's penetration ability based on this distance. The farther the sperm travels within the gel, the greater the sperm's ability to penetrate the gel.

[0045] The sperm penetration ability detection method can also measure the farthest swimming distance of the sperm in the gel through the scale in the detection chamber 3; calculate the first average number of sperm at half of the farthest swimming distance, and compare the first average number with the second average number of sperm at the same distance in the control group to obtain the percentage of the first average number to the second average number; then determine the sperm penetration ability in the gel based on the percentage.

[0046] The first average is calculated by photographing motile sperm in five fields of view at half the maximum distance of sperm migration within the detection chamber 3 under a microscope, and calculating the average number of motile sperm in the images (a motile sperm with both its head and tail fully photographed is counted as one sperm). This average is the first average. The second average is calculated by photographing motile sperm in five fields of view at the same distance from the control group under a microscope, and calculating the average number of motile sperm in the images (a motile sperm with both its head and tail fully photographed is counted as one sperm). This average is the second average.

[0047] In one embodiment, the maximum distance sperm in the gel group's detection chamber 3 migrated was 17 mm. At half the maximum distance (8.5 mm), the first average number of motile sperm was approximately 26. In the control group's detection chamber 3C, at the same location (8.5 mm), the second average number of motile sperm was approximately 56. The percentage of the first average number to the second average number was 46.43. A smaller percentage indicates weaker sperm penetration in the gel group, while a larger percentage indicates stronger sperm penetration in the gel group.

[0048] Some gels may contain drug ingredients that kill sperm or inhibit sperm motility. Sperm cannot move or survive in the gel. Therefore, no active sperm may be observed in the detection chamber 3 containing the gel. In this case, the first average number of active sperm is directly recorded as 0, and the above percentage is also recorded as 0.

[0049] Example 2

[0050] In this embodiment, the method for using the sperm penetration ability detection device includes the following steps:

[0051] S1. Take liquefied semen and perform upstream test to obtain high-motility sperm, then adjust the sperm concentration to 1×10 7 / ml, as a backup;

[0052] S2. Incubate the sperm from the previous step with the drug (a drug that inhibits or promotes sperm motility) for 10-120 minutes, centrifuge at 300 g for 5 minutes, discard the supernatant, and rinse 2-3 times with culture medium (containing 1% bovine serum albumin) for later use. Add artificial cervical mucus from the front end of detection chamber 3 to sample reservoir 5. Siphon the chamber 3 until it is filled with artificial cervical mucus.

[0053] S3. Use vaseline or other sperm-safe sealant to seal the rear opening of the detection chamber 3;

[0054] S4. Add 3 to 5 μl of sperm from step S2 to the sperm reservoir 4 in the detection chamber 3;

[0055] S5. Place the sperm penetration test device in a humidified chamber and incubate at 37°C in a 5% CO2 incubator for 10-30 min.

[0056] S6. Observe under a microscope and record the presence of sperm, sperm count, and maximum distance of sperm movement in chamber 3;

[0057] S7. Compare the data recorded in step S6 with a control group to determine the barrier effect of the gel on sperm. The control group uses the same sperm penetration ability testing device as described above, except that the sperm are incubated with culture medium (containing 1% bovine serum albumin) instead of the drug.

[0058] According to the description of the method for using the sperm penetration ability detection device above, a more universal sperm penetration ability detection method can be obtained, which includes the following steps:

[0059] Step 1: Determine the swimming direction of sperm;

[0060] This step can be achieved through the above-mentioned detection chamber 3, and the swimming direction of the sperm is determined to be along the length direction of the sample loading groove 5.

[0061] Step 2: placing artificial cervical mucus in the direction of sperm swimming;

[0062] This step can be achieved by adding artificial cervical mucus to fill the detection chamber 3 .

[0063] Step three, testing the penetration of the drug-treated sperm into the artificial cervical mucus to determine the penetration ability of the drug-treated sperm in the artificial cervical mucus.

[0064] This step can be accomplished by observing the position of sperm in the artificial cervical mucus under a microscope. Specifically, the sperm penetration ability test method uses a scale to measure the distance sperm travel within the artificial cervical mucus after drug treatment, and then determines the sperm's penetration ability based on this distance. The greater the distance sperm travels within the artificial cervical mucus after drug treatment, the greater the sperm's penetration ability within the artificial cervical mucus.

[0065] The sperm penetration ability detection method can also measure the farthest swimming distance of the sperm in the artificial cervical mucus after drug treatment through the scale in the detection chamber 3; calculate the first average number of sperm at half of the farthest swimming distance, and compare the first average number with the second average number of sperm at the same distance in the control group to obtain the percentage of the first average number to the second average number; then determine the penetration ability of the sperm in the artificial cervical mucus after drug treatment based on the percentage.

[0066] The first average is calculated by photographing motile sperm in five fields of view at half the maximum distance of sperm migration within the detection chamber 3 under a microscope, and calculating the average number of motile sperm in the images (a motile sperm with both its head and tail fully photographed is counted as one sperm). This average is the first average. The second average is calculated by photographing motile sperm in five fields of view at the same distance from the control group under a microscope, and calculating the average number of motile sperm in the images (a motile sperm with both its head and tail fully photographed is counted as one sperm). This average is the second average.

[0067] In one embodiment, the maximum swimming distance of sperm in the gel group detection chamber 3 was 13 mm. At half the maximum distance (6.5 mm), the first average number of motile sperm was approximately 36. The second average number of motile sperm at the same location (6.5 mm) in the control group detection chamber 3 was approximately 66. The percentage of the first average number to the second average number was 54.54. A smaller percentage indicates a greater effect of the drug on sperm penetration, while a larger percentage indicates a smaller effect of the drug on sperm penetration.

[0068] Certain drugs may contain ingredients that kill sperm or inhibit sperm motility. After drug treatment, sperm can no longer move in artificial cervical mucus. Therefore, motile sperm may not be observed in detection chamber 3C containing artificial cervical mucus. In this case, the first average number of motile sperm is directly recorded as 0, and the above percentage is also recorded as 0.

[0069] Certain drugs may contain ingredients that promote sperm motility. After drug treatment, sperm penetration ability is enhanced. It may be observed that the maximum sperm swimming distance in testing chamber 3C containing artificial cervical mucus is greater than that in the control group, or the first average is greater than the second average. Therefore, the percentage of the first average to the second average may be greater than 100%.

[0070] This embodiment is only a further explanation of the invention and is not a limitation of the invention. After reading this specification, those skilled in the art may make non-creative modifications to this embodiment as needed, but as long as they are within the scope of the claims of the invention, they are protected by patent law.

Claims

1. A sperm penetration ability detection device, characterized in that: The invention comprises a glass cover and a glass base. The glass base is provided with a whitening area and a detection area. The glass cover covers the detection area. The detection area is evenly divided into several independent detection chambers. The detection chambers are provided with interconnected sample loading grooves and sperm storage tanks.

2. The sperm penetration ability detection device according to claim 1, characterized in that: The glass cover piece is 50-80 mm long, 20-25 mm wide, and 0.15-0.2 mm thick. There is a gap of 0.01-0.1 mm between the glass cover piece and the glass base.

3. The sperm penetration ability detection device according to claim 1, characterized in that: The detection area is evenly divided into 3-5 independent detection chambers by epoxy resin.

4. The sperm penetration ability detection device according to claim 3, characterized in that: The detection chamber is 15 to 20 mm wide and is open at both ends. A sample adding groove is provided in the middle of the detection chamber. A sperm storage pool is engraved in the middle of the front end of the detection chamber. The front end of the sample adding groove is connected to the sperm storage pool, and the gel sample is added to the sample adding groove.

5. The sperm penetration ability detection device according to claim 4, characterized in that: The sperm storage pool is a 1 / 4 spherical groove with a spherical radius of 0.7-1.2 mm. The sample adding groove is 15-20 mm long and 0.2-0.4 mm deep. The sample adding groove is engraved on the glass base.

6. The sperm penetration ability detection device according to claim 5, characterized in that: Scale bars are provided on both sides of the sample adding groove, the scale bars are 2-4 mm away from the sample adding groove, the scale bars are 20-25 mm long, and the minimum scale is 0.1 mm.

7. The sperm penetration ability detection device according to claim 1, characterized in that: A separation strip is provided at the edge of the detection chamber.

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