Optical imaging system
By integrating the Hoffman imaging system and the Mueller matrix polarization imaging system, the problem of image misalignment caused by sample movement is solved, highly fused synchronous imaging is achieved, and the characteristic analysis capability of cell/embryo imaging is improved.
Patent Information
- Application Number
- CN202422453653.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-11
- Publication Date
- 2025-09-26
- Estimated Expiration
- 2034-10-11
AI Technical Summary
Existing cell/embryo imaging equipment requires moving samples over long distances when using Hoffman imaging technology and Mueller matrix polarization imaging technology, resulting in image overlap and affecting feature analysis.
The Hoffman imaging system and the Mueller matrix polarization imaging system are integrated into an optical imaging system, and synchronous imaging is achieved through a beam splitter prism to ensure that the two systems image the same sample at the same time, avoiding the problem of image misalignment caused by sample movement.
The two imaging methods achieve a high degree of correspondence in time and space, and the image feature fusion degree is high, which improves the accuracy of sample feature analysis.
Smart Images
Figure CN223389655U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of microscopy technology, in particular to an optical imaging system. Background Art
[0002] In the field of microscopy, brightfield illumination is commonly used to directly observe samples. However, this observation method is not ideal when applied to cells / embryos. This is because cells / embryos are transparent and colorless sample tissues, so the images obtained using brightfield illumination often have very low contrast. Therefore, Hoffman imaging technology is often used in this field to enhance the contrast of cell / embryo images. In addition, because cells / embryos also contain tissues with polarization characteristics such as spindles, Mueller matrix polarization imaging technology is also chosen for imaging.
[0003] Existing imaging equipment for cells / embryos typically uses either Hoffman imaging technology or Mueller matrix polarization imaging technology. If you want to obtain imaging data from both, you need to move the sample over a long distance, which will cause changes in the sample tissue and affect the final observation results. Utility Model Content
[0004] In order to solve the problems existing in the prior art, the utility model discloses an optical imaging system.
[0005] An optical imaging system includes a Hoffman imaging system and a Mueller matrix polarization imaging system. By arranging a plurality of beam splitting prisms, the Hoffman imaging system and the Mueller matrix polarization imaging system can synchronously image the same sample imaging area.
[0006] Specifically, the Hoffman imaging system and the Mueller matrix polarization imaging system are integrated into a single optical imaging system, allowing both to image the same sample at the same time. This effectively eliminates the problem of the two images not overlapping due to sample movement, which in turn affects feature analysis. That is, with this system, the image features obtained by the two imaging methods are highly aligned in time and space, and have a high degree of fusion, which is more conducive to analyzing the characteristics of the sample.
[0007] In addition, samples are usually transparent and colorless tissues such as embryos and cells.
[0008] Preferably, the Hoffman imaging system comprises a first light source unit and a first imaging unit, and the sample is arranged between the first light source unit and the first imaging unit;
[0009] The Mueller matrix polarization imaging system includes a second light source section and a second imaging section, and the sample is arranged between the second light source section and the second imaging section;
[0010] The plurality of beam splitter prisms include a first beam splitter prism disposed between the sample and the first light source portion, and a second beam splitter prism disposed between the sample and the second imaging portion;
[0011] The first beam splitter prism and the second beam splitter prism are arranged on a straight line;
[0012] The position of the first beam splitter prism does not affect the imaging of the Hoffman imaging system and the first beam splitter prism is on the optical path of the Mueller matrix polarization imaging system;
[0013] The second beam splitter prism is disposed on the optical path of the Hoffman imaging system, and the second imaging portion is located on the optical path of the light reflected by the second beam splitter prism.
[0014] Specifically, the Hoffman imaging system and the Mueller matrix imaging system are integrated into an optical imaging system. The two need to be able to image the same sample at the same time, so the optical paths between the two cannot conflict. This setting can achieve the above-mentioned function.
[0015] Preferably, the first light source unit is provided with a Hoffman light source, a light collecting mirror, a first polarizer, a slit plate and a condenser in a direction close to the sample, and the first beam splitter prism is arranged between the condenser and the sample.
[0016] Specifically, the Hoffman light source beam provided by the Hoffman light source passes through a collecting mirror and reaches the first polarizer. The first polarizer changes the Hoffman light source beam from natural light into linearly polarized light, and then the linearly polarized light reaches the slit plate and the condenser in sequence; the condenser focuses the linearly polarized light and tilts the linear polarized light at a certain angle (usually from one side downward) to avoid the first beam splitter prism and then illuminate the sample. That is, the first beam splitter prism is not in the optical path of the Hoffman imaging system; in addition, the first beam splitter prism is a small-sized beam splitter prism and is a non-polarizing beam splitter prism.
[0017] Preferably, the first imaging portion is provided with a Hoffman objective lens, a first filter, a first tube lens and a Hoffman camera in a direction away from the sample, and the second beam splitter prism is arranged between the Hoffman objective lens and the first filter.
[0018] Specifically, the Hoffman objective lens is combined with a condenser and a slit to realize Hoffman modulation imaging, so that the imaging of the sample presents a relief effect; the first filter transmits the Hoffman light source beam and cuts off the Mueller light source beam; the first tube lens is used to focus the imaging light of the Hoffman objective lens onto the CMOS chip of the Hoffman camera; after receiving the imaging light, the Hoffman camera collects signals to generate a Hoffman imaging image.
[0019] Preferably, the Hoffman light source is a Hoffman green light source, and the first filter is a green light filter.
[0020] Preferably, the second light source unit is provided with a Mueller light source, a collimating lens, a second polarizer, a rotating motor, and a first quarter wave plate toward the direction close to the first beam splitter prism, and the rotating motor is used to adjust the angle between the first quarter wave plate and the first beam splitter prism.
[0021] Specifically, the Mueller light source beam provided by the Mueller light source is converted into a parallel beam after passing through a collimating lens, and then the second polarizer converts the parallel beam from natural light into linearly polarized light. The angle of the first quarter-wave plate is adjusted by a rotating motor to change the polarization state of the linearly polarized light as needed. The linearly polarized light is then reflected by the first beam splitter prism and irradiated onto the sample.
[0022] Preferably, the second imaging unit is provided with a second filter, a second tube lens, a third beam splitter prism, a second quarter-wave plate, and a first polarization camera in a direction away from the second beam splitter prism; the second imaging unit also includes a second polarization camera, and the second polarization camera is arranged on the optical path of the light reflected by the third beam splitter prism.
[0023] Specifically, the Mueller light source beam emitted by the Mueller light source undergoes the aforementioned changes and is then irradiated onto the sample. It is then magnified by the Hoffman objective lens and irradiated onto the second beam splitter prism. The second beam splitter prism reflects the light, passes the light through a second filter, and then reaches the second tube lens. The second tube lens focuses the light and irradiates the light onto the third beam splitter prism. Part of the light irradiating the third beam splitter prism is transmitted through the third beam splitter prism, and the other part is reflected by the third beam splitter prism. The part of the light that has transmitted the third beam splitter prism passes through the second quarter-wave plate and is focused onto the first polarization camera. The light reflected by the third beam splitter prism is then focused onto the second polarization camera. Images are formed by the first polarization camera and the second polarization camera, respectively.
[0024] The second filter transmits the Mueller light source beam and cuts off the Hoffman light source beam.
[0025] Preferably, the Mueller light source is a Mueller red light source, and the second filter is a red light filter.
[0026] Preferably, the system further includes an image fusion unit for acquiring images from the Hoffman imaging system and the Mueller matrix polarization imaging system and fusing the two images for output.
[0027] Compared with the prior art, the present invention is beneficial in that:
[0028] The utility model can simultaneously perform Hoffman imaging and Mueller matrix polarization imaging. During the imaging process, the sample, light source, camera and other components do not need to be moved. The light source and camera are directly turned on by electrical signals to collect images to obtain images of the two imaging technologies. The images of the two imaging technologies can then be fused. Since the two imaging methods are performed simultaneously on the same sample, the features of the two images obtained are highly corresponding in time and space, so the degree of fusion of the two images is extremely high. BRIEF DESCRIPTION OF THE DRAWINGS
[0029] Figure 1 A schematic diagram of the overall structure of the optical imaging system provided by the present invention (excluding the optical path);
[0030] Figure 2 A schematic diagram of the partial structure of the optical imaging system provided by the present invention (including part of the optical path);
[0031] Figure 3 This is an example picture of Hoffman imaging of the optical imaging system provided by the utility model;
[0032] Figure 4 This is an example picture of Mueller matrix polarization imaging of the optical imaging system provided by the utility model;
[0033] Figure 5 This is an example picture of the fused image of the optical imaging system provided by the present invention. DETAILED DESCRIPTION
[0034] The present invention will be further described below with reference to the accompanying drawings and specific embodiments.
[0035] like Figure 1-2 The optical imaging system shown includes a Hoffman imaging system and a Mueller matrix polarization imaging system. By setting a plurality of beam splitting prisms, the Hoffman imaging system and the Mueller matrix polarization imaging system can simultaneously image the imaging area of the same sample 22.
[0036] The Hoffman imaging system and the Mueller matrix polarization imaging system are integrated into a single optical imaging system, and both can image the same sample 22 at the same time. This effectively eliminates the problem of the two images not being able to overlap due to the movement of the sample 22, which in turn affects feature analysis. That is, with this system, the image features obtained by the two imaging methods are highly consistent in time and space, and the degree of fusion is high, which is more conducive to the analysis of the characteristics of the sample 22.
[0037] In addition, the sample 22 is usually a transparent and colorless tissue such as an embryo or a cell.
[0038] The Hoffman imaging system includes a first light source section and a first imaging section, and the sample 22 is disposed between the first light source section and the first imaging section;
[0039] The Mueller matrix polarization imaging system includes a second light source section and a second imaging section, and the sample 22 is disposed between the second light source section and the second imaging section;
[0040] The plurality of beam splitters include a first beam splitter prism 21 disposed between the sample 22 and the first light source portion, and a second beam splitter prism 23 disposed between the sample 22 and the second imaging portion;
[0041] The first beam splitter prism 21 and the second beam splitter prism 23 are arranged on a straight line;
[0042] The position of the first beam splitter prism 21 does not affect the optical path of the Hoffman imaging system and the first beam splitter prism 21 is on the optical path of the Mueller matrix polarization imaging system;
[0043] The second beam splitter prism 23 is disposed on the optical path of the Hoffman imaging system, and the second imaging portion is located on the optical path of the light reflected by the second beam splitter prism 23 .
[0044] The Hoffman imaging system and the Mueller matrix imaging system are integrated into one optical imaging system. The two need to be able to image the same sample 22 at the same time, so the optical paths between the two cannot conflict. This setting can achieve the above-mentioned function.
[0045] The first light source unit is provided with a Hoffman light source 11 , a condenser lens 12 , a first polarizer 13 , a slit plate 14 and a condenser lens 15 toward the sample 22 , and a first beam splitter prism 21 is provided between the condenser lens 15 and the sample 22 .
[0046] The Hoffman light source beam 61 provided by the Hoffman light source 11 passes through the condenser 12 and reaches the first polarizer 13. The first polarizer 13 changes the Hoffman light source beam 61 from natural light into linearly polarized light, and then the linearly polarized light reaches the slit 14 and the condenser 15 in sequence; the condenser 15 focuses the linearly polarized light and tilts the linear polarized light at a certain angle (usually from one side downward) to avoid the first beam splitter prism 21 and then illuminates the sample 22. That is, the first beam splitter prism 21 is not in the optical path of the Hoffman imaging system; in addition, the first beam splitter prism 21 is a small-sized beam splitter prism and is a non-polarizing beam splitter prism.
[0047] The first imaging portion is provided with a Hoffman objective lens 31 , a first filter 32 , a first tube lens 33 and a Hoffman camera 34 in a direction away from the sample 22 , and the second beam splitter prism 23 is arranged between the Hoffman objective lens 31 and the first filter 32 .
[0048] The Hoffman objective lens 31 is combined with the condenser 15 and the slit 14 to realize Hoffman modulation imaging, so that the imaging of the sample 22 presents a relief effect; the first filter 32 transmits the Hoffman light source beam 61 and cuts off the Mueller light source beam 62; the first tube lens 33 is used to focus the imaging light of the Hoffman objective lens 31 onto the CMOS chip of the Hoffman camera 34; after receiving the imaging light, the Hoffman camera 34 collects signals to generate a Hoffman imaging image.
[0049] The Hoffman light source 11 is a Hoffman green light source, and the first filter 32 is a green light filter.
[0050] The second light source unit is provided with a Mueller light source 41, a collimating lens 42, a second polarizer 43, a rotating motor 44, and a first quarter wave plate 45 toward the direction close to the first beam splitter prism 21. The rotating motor 44 is used to adjust the angle between the first quarter wave plate 45 and the first beam splitter prism 21.
[0051] The Mueller light source beam 62 provided by the Mueller light source 41 is converted into a parallel light beam after passing through the collimating lens 42, and then the second polarizer 43 converts the parallel light beam from natural light into linearly polarized light. The angle of the first quarter-wave plate 45 is adjusted by the rotating motor 44 to change the polarization state of the linearly polarized light as needed, and then the linearly polarized light is reflected by the first beam splitter prism 21 and irradiated onto the sample 22.
[0052] The second imaging unit is provided with a second filter 51, a second tube lens 52, a third beam splitter prism 53, a second quarter-wave plate 54, and a first polarization camera 55 in a direction away from the second beam splitter prism 23; the second imaging unit also includes a second polarization camera 56, which is arranged on the optical path of the light reflected by the third beam splitter prism 53.
[0053] The Mueller light source beam 62 emitted by the Mueller light source 41 undergoes the aforementioned changes and impinges on the sample 22. It is then magnified by the Hoffman objective lens 31 and impinges on the second beam splitter prism 23. The second beam splitter prism 23 reflects the light, passes through the second filter 51, and then reaches the second tube lens 52. The second tube lens 52 focuses the light and impinges on the third beam splitter prism 53. Part of the light impinging on the third beam splitter prism 53 is transmitted through the third beam splitter prism 53, and the other part is reflected by the third beam splitter prism 53. The part of the light that has transmitted through the third beam splitter prism 53 passes through the second quarter-wave plate 54 and is focused on the first polarization camera 55. The light reflected by the third beam splitter prism 53 is focused on the second polarization camera 56. The first polarization camera 55 and the second polarization camera 56 respectively form images.
[0054] The second filter 51 transmits the Mueller light source light beam 62 and cuts off the Hoffman light source light beam 61 .
[0055] The Mueller light source 41 is a Mueller red light source, and the second filter 51 is a red light filter.
[0056] The system also includes an image fusion unit for acquiring images from the Hoffman imaging system and the Mueller matrix polarization imaging system and fusing the two for output.
[0057] The image obtained by the Hoffman imaging system is as follows Figure 3 As shown, the image obtained by the Mueller matrix polarization imaging system is as follows Figure 4 As shown, the image obtained after the two are fused is as follows Figure 5 shown.
Claims
1. An optical imaging system, comprising a Hoffman imaging system and a Mueller matrix polarization imaging system, characterized in that: By setting a plurality of beam splitting prisms, the Hoffman imaging system and the Mueller matrix polarization imaging system can synchronously image the same sample imaging area.
2. The imaging system according to claim 1, wherein: The Hoffman imaging system includes a first light source unit and a first imaging unit, and the sample is arranged between the first light source unit and the first imaging unit; The Mueller matrix polarization imaging system includes a second light source section and a second imaging section, and the sample is arranged between the second light source section and the second imaging section; The plurality of beam splitter prisms include a first beam splitter prism disposed between the sample and the first light source portion, and a second beam splitter prism disposed between the sample and the second imaging portion; The first beam splitter prism and the second beam splitter prism are arranged on a straight line.
3. The imaging system according to claim 2, wherein: The position of the first beam splitter prism does not affect the imaging of the Hoffman imaging system and the first beam splitter prism is on the optical path of the Mueller matrix polarization imaging system; The second beam splitter prism is disposed on the optical path of the Hoffman imaging system, and the second imaging portion is located on the optical path of the light reflected by the second beam splitter prism.
4. The imaging system according to claim 3, wherein: The first light source unit is provided with a Hoffman light source, a condenser, a first polarizer, a slit plate and a condenser toward the direction close to the sample, and the first beam splitter prism is arranged between the condenser and the sample.
5. The imaging system according to claim 4, wherein: The first imaging part is provided with a Hoffman objective lens, a first filter, a first tube lens and a Hoffman camera in a direction away from the sample, and the second beam splitter prism is arranged between the Hoffman objective lens and the first filter.
6. The imaging system according to claim 5, wherein: The Hoffman light source is a Hoffman green light source, and the first filter is a green light filter.
7. The imaging system according to claim 3, wherein: The second light source unit is provided with a Mueller light source, a collimating lens, a second polarizer, a rotating motor, and a first quarter wave plate toward the direction close to the first beam splitter prism. The rotating motor is used to adjust the angle between the first quarter wave plate and the first beam splitter prism.
8. The imaging system according to claim 7, wherein: The second imaging unit is provided with a second filter, a second tube lens, a third beam splitter prism, a second quarter-wave plate, and a first polarization camera in a direction away from the second beam splitter prism; the second imaging unit also includes a second polarization camera, which is arranged on the optical path of the light reflected by the third beam splitter prism.
9. The imaging system according to claim 8, wherein: The Mueller light source is a Mueller red light source, and the second filter is a red light filter.
10. The imaging system according to claim 1, wherein: The system also includes an image fusion unit for acquiring images from the Hoffman imaging system and the Mueller matrix polarization imaging system and fusing the two for output.