WB experiment antibody incubation box capable of being used for simultaneously incubating multiple membranes

By designing a WB antibody incubation box with a movable baffle, the problem of adhesion during multi-membrane incubation was solved, achieving uniform antibody distribution and saving antibody, thus simplifying experimental procedures.

CN223471041UActive Publication Date: 2025-10-24ZHONGSHAN HOSPITAL FUDAN UNIV
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Patent Information

Application Number
CN202422884563.3
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-11-25
Publication Date
2025-10-24
Estimated Expiration
2034-11-25

AI Technical Summary

Technical Problem

In existing technologies, multiple membranes tend to stick together during antibody incubation, leading to uneven antibody binding, which affects experimental results. Furthermore, multiple antibody incubation boxes are required, resulting in antibody waste.

Method used

Design a Western blot antibody incubation box for simultaneous incubation of multiple membranes, comprising a box body, a box lid, and a movable baffle. The baffle is equipped with locking blocks and channels, and its position can be adjusted to accommodate different membrane sizes. A grid-type or sieve-type baffle is used to ensure uniform antibody distribution.

Benefits of technology

This allows for simultaneous incubation of multiple membranes, avoiding adhesion, saving antibody usage, and improving experimental efficiency and effectiveness.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model discloses a WB experiment antibody incubation box capable of being used for simultaneously incubating a plurality of membranes. The antibody incubation box comprises a box body, a box cover and a movable baffle, the movable baffle is movably installed in the box body, a set of protruding clamping blocks with the same shape and size are arranged on the two corresponding sides of the upper end of the movable baffle, and the box body comprises a bottom plate and four side plates arranged around the bottom plate; transverse grooves distributed at intervals in the same direction are formed in the inner wall of the bottom plate, a plurality of sets of vertical grooves corresponding in size and position are formed in the inner walls of side plates on the two corresponding sides of the box body, the vertical grooves and the transverse grooves correspond in position and are distributed at intervals in the same direction, and the transverse grooves are used for being connected with the bottoms of movable baffles in an inserted mode. The vertical groove is used for being connected with a clamping block of a movable baffle in a clamped mode, and the movable baffle is a grating type baffle or a sieve hole type baffle. According to the utility model, when a plurality of WB membranes are incubated by using the same antibody, aggregation of the plurality of membranes and nonuniform antibody combination can be effectively avoided, and the use of the antibody can be reduced.
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Description

TECHNICAL FIELD

[0001] The utility model relates to a WB experiment antibody incubation box for simultaneous incubation of multiple membranes belongs to biological experiment instrument technical field. BACKGROUND

[0002] Western blotting (WB) is a method for detecting the content of specific protein in a sample in biological experiments. The main steps include sample extraction, electrophoresis, membrane transfer, antibody incubation and development. The experimental steps are complex, and any operation step will affect the experimental results. Among them, antibody incubation is a very key step, and whether the antibody incubation is sufficient will directly affect the experimental results. Usually in the incubation process, if two or more different membranes need to be incubated in the same antibody, an antibody incubation box is needed for each membrane, because if multiple membranes are incubated in the same antibody incubation box, the multiple membranes will be concentrated together and stick to each other, resulting in insufficient and uneven antibody binding, affecting the experimental results. SUMMARY

[0003] The utility model aims at: in order to solve above -mentioned problem, the utility model provides a WB experiment antibody incubation box for simultaneous incubation of multiple membranes, can better solve the problem that multiple membranes use the same antibody incubation needs multiple antibody incubation boxes, can save the antibody while simplifying the experimental operation.

[0004] In order to achieve the above object, the utility model provides a WB experiment antibody incubation box for simultaneous incubation of multiple membranes, including box body, box cover and movable baffle, the movable baffle is movably installed in the box body, a group of protruding and the same shape and size of clamping block are arranged on the upper end of the movable baffle, the box body includes the bottom plate and the four side plates arranged around the bottom plate, the inner wall of the bottom plate is provided with the transverse slot spaced apart along the same direction, the inner wall of the corresponding two side plates of the box body is provided with a plurality of groups of vertical slots corresponding in size and position, the vertical slot and the transverse slot are positionally corresponding and spaced apart along the same direction, the transverse slot is used for the bottom of the movable baffle and is inserted, the vertical slot is used for the clamping block of the movable baffle and is clamped, the movable baffle is provided with the hole for liquid circulation.

[0005] Preferably, the movable baffle is a grid baffle or a mesh baffle, allowing the liquid on both sides of the baffle to flow.

[0006] Preferably, the length of the transverse slot and the bottom of the movable baffle is set to be consistent, and the shape and length of the vertical slot and the clamping block are set to be consistent.

[0007] Compared with the prior art, the utility model has the beneficial effects that:

[0008] The WB experimental antibody incubation box for simultaneously incubating multiple films can be used, the movable baffle is provided with a grid type or a screen hole type baffle, the position of the baffle can be adjusted in real time according to experimental requirements, when the same antibody is used to incubate different films, on one hand, multiple films can be prevented from being pasted together, and uneven antibody combination can be avoided, on the other hand, the amount of the antibody used can be saved, efficient incubation of multiple films can be completed in one experiment, and the experimental effect is improved and the consumption of the antibody is saved BRIEF DESCRIPTION OF DRAWINGS

[0009] Figure 1 The whole structure schematic view of the WB experimental antibody incubation box provided for the embodiment is shown in the figure.

[0010] Figure 2 The inside structure schematic view of the box body is shown in the figure.

[0011] Figure 3 The structure schematic view of the grid type baffle is shown in the figure.

[0012] Figure 4 The structure schematic view of the screen hole type baffle is shown in the figure.

[0013] The figure shows that the box body, the box cover and the movable baffle are shown in the figure. DETAILED DESCRIPTION

[0014] In order to make the utility model more obvious and easy to understand, the preferred embodiment is described in detail below, and the figure is used for description.

[0015] Embodiment 1

[0016] The WB experimental antibody incubation box for simultaneously incubating multiple films can be used, the movable baffle is provided with a grid type or a screen hole type baffle, the position of the baffle can be adjusted in real time according to experimental requirements, when the same antibody is used to incubate different films, on one hand, multiple films can be prevented from being pasted together, and uneven antibody combination can be avoided, on the other hand, the amount of the antibody used can be saved, efficient incubation of multiple films can be completed in one experiment, and the experimental effect is improved and the consumption of the antibody is saved Figures 1-4 The WB experimental antibody incubation box for simultaneously incubating multiple films can be used, the movable baffle is provided with a grid type or a screen hole type baffle, the position of the baffle can be adjusted in real time according to experimental requirements, when the same antibody is used to incubate different films, on one hand, multiple films can be prevented from being pasted together, and uneven antibody combination can be avoided, on the other hand, the amount of the antibody used can be saved, efficient incubation of multiple films can be completed in one experiment, and the experimental effect is improved and the consumption of the antibody is saved Figure 3 ) or a screen hole type baffleFigure 4 ), allowing the liquid on both sides of the baffle to communicate, in the experiment, the antibody can be added at one time, and the uniform distribution of the antibody in the antibody incubation box can be ensured. In the utility model, the overall size of the antibody incubation box can be designed according to needs, and meanwhile, one or more movable baffles can be selected to be inserted into the clamping groove of the antibody incubation box according to experimental needs, and the movable baffle can be moved and its mounting position can be adjusted according to the size of the incubation film, and the size of the incubation space of the antibody incubation box can be adjusted according to the size of the incubation film.

[0017] The above implementation cases are only preferred implementation cases of the utility model, and do not limit the utility model in any form and substance, and it should be pointed out that, for ordinary skilled persons in the technical field, some improvements and supplements can be made without departing from the utility model, and these improvements and supplements should also be regarded as the protection scope of the utility model.

Claims

1. A WB experiment antibody incubation box which can be used for simultaneous incubation of multiple membranes, characterized in that, The utility model relates to a box, which comprises a box body, a box cover and a movable baffle, the movable baffle is movably installed in the box body, the upper end of the movable baffle is provided with a group of convex and same shape and size clamping blocks on the corresponding sides, the box body comprises a bottom plate and four side plates arranged around the bottom plate, the inner wall of the bottom plate is provided with transverse grooves which are spaced apart along the same direction, the inner wall of the corresponding two side plates of the box body is provided with a plurality of groups of vertical grooves which are corresponding in size and position, the vertical grooves and the transverse grooves are spaced apart along the same direction and correspond in position, the transverse grooves are used for being inserted with the bottom of the movable baffle, the vertical grooves are used for being clamped with the clamping blocks of the movable baffle, and the movable baffle is provided with a channel for liquid circulation.

2. The WB experimental antibody incubation box of claim 1, wherein, The movable baffle is a grating baffle or a mesh baffle.

3. The WB experimental antibody incubation box of claim 1 or 2, wherein, The length of the transverse grooves and the bottom of the movable baffle is consistent, and the shape and length of the vertical grooves and the clamping blocks are consistent.