Nucleic acid synthesis gas phase ammonolysis device

Abstract

The utility model discloses a nucleic acid synthesis gas phase ammonolysis device, which relates to the technical field of nucleic acid synthesis gas phase ammonolysis, and comprises a device main body and a clamping frame, the bottom of the clamping frame is connected with a push plate, the top of the device main body is provided with a motor, the output end of the motor is connected with a rotating shaft, and the outer surface of the rotating shaft is respectively connected with a paddle and a fan blade. Through the arrangement of the paddle, the fan blade, the clamping frame and the push plate, the motor drives the paddle and the fan blade to simultaneously rotate at a high speed, the fan blade rotating at the high speed generates airflow to increase the contact between ammonia gas and the nucleic acid synthesis column, and the paddle rotates at the high speed and stirs water flow, so that water can be uniformly heated, the output efficiency of water vapor is improved, and the production efficiency of the nucleic acid synthesis column is improved. The water flow can generate vortexes while being stirred by the paddles, and the arc-shaped push plate has relatively large water resistance, so that the vortexes can drive the clamping frame to rotate, and the nucleic acid synthesis column is driven to rotate; the ammonolysis reaction efficiency is improved, and the influence on the nucleic acid synthesis column caused by overlarge centrifugal force is avoided.

Description

Technical Field

[0001] This utility model relates to the field of gas-phase ammonolysis technology for nucleic acid synthesis, specifically to an apparatus for gas-phase ammonolysis of nucleic acid synthesis. Background Technology

[0002] After oligonucleotide synthesis, the synthesized oligonucleotide chain needs to be chemically cleaved from the support. The main method now is to use concentrated ammonia or similar substances to break the ester bond between the oligonucleotide compound and the initial nucleoside on the solid-phase synthesis support, which is an important step in oligonucleotide synthesis.

[0003] A device for large-scale nucleic acid synthesis gas-phase ammonolysis, application number 202120442309.1, includes an ammonolysis tank. The ammonolysis tank is a cylindrical vessel with an inner cavity. A driving mechanism is located at the bottom of the inner cavity, and a clamping mechanism is located in the middle of the inner cavity. A sealing top cover is hinged to the top of the ammonolysis tank. A spraying mechanism, a pressure gauge, and an air inlet mechanism are embedded in the sealing top cover. The pressure gauge is located between the spraying mechanism and the air inlet mechanism. A water pool is located at the bottom of the inner cavity of the ammonolysis tank. Several heating plates are located on the sidewalls of the inner cavity of the ammonolysis tank, within the water pool. A water outlet pipe with a switch is located at the bottom of the water pool. Furthermore, by incorporating the clamping device, this invention allows for the placement of multiple nucleic acid synthesis columns within the ammonolysis device, enabling simultaneous ammonolysis and subsequent purification of multiple nucleic acid columns, thus improving the working efficiency of the ammonolysis device.

[0004] This technical solution uses a motor to drive the fan blades and the nucleic acid synthesis column in the clamping mechanism to rotate. However, the fan blades need to rotate at a high speed, otherwise it will be difficult to generate airflow or the generated airflow will be small, thus affecting the airflow circulation and contact with the nucleic acid synthesis column. However, if the speed is too high, it will drive the nucleic acid synthesis column to rotate rapidly. Since the nucleic acid synthesis column is a solid structure, high-speed rotation will generate a large centrifugal force, which will have a significant impact on the clamping stability and structural stability of the nucleic acid synthesis column. Utility Model Content

[0005] Based on this, the purpose of this utility model is to provide an apparatus for gas-phase ammonolysis of nucleic acid synthesis, so as to solve the technical problems mentioned in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a device for gas-phase ammonolysis of nucleic acid synthesis, comprising a device body and a clamping frame, wherein a push plate is connected to the bottom of the clamping frame, a motor is installed on the top of the device body, and a rotating shaft is connected to the output end of the motor, wherein a paddle blade and a fan blade are respectively connected to the outer surface of the rotating shaft.

[0007] By adopting the above technical solution, the high-speed rotating blades generate airflow to increase the contact between ammonia and the nucleic acid synthesis column. The high-speed rotation of the blades agitates the water flow, thereby enabling the water to be heated evenly and improving the steam production efficiency. At the same time, the water flow is agitated by the blades and generates eddies. The arc-shaped pusher plate has a large water resistance, which allows the eddies to drive the clamping frame to rotate, thereby driving the nucleic acid synthesis column to rotate, further increasing the contact between ammonia and the nucleic acid synthesis column. The high-speed rotation of the blades generates eddies in the water, which drive the clamping frame to rotate. During this process, there is a large energy consumption. The number of pushers is small and the contact area with the water flow is small, so the rotation speed of the clamping frame is much lower than that of the shaft. This avoids the phenomenon of loosening of the clamping and structural breakage due to excessive rotation speed of the clamping frame and the nucleic acid synthesis column.

[0008] Furthermore, the push plate is arc-shaped, and the clamping frame is rotatably connected to the main body of the device.

[0009] By adopting the above technical solution, the arc-shaped pusher plate has a large water resistance, which enables the vortex to drive the clamping frame to rotate, thereby driving the nucleic acid synthesis column to rotate, and further increasing the contact between ammonia and the nucleic acid synthesis column.

[0010] Furthermore, four push plates are provided, and the four push plates are distributed in a circular array.

[0011] By adopting the above technical solution, the high-speed rotation of the blades generates eddies in the water, which in turn drive the clamping frame to rotate. During this process, there is a significant energy consumption, and the number of push plates is small, resulting in a small contact area with the water flow. This ensures that the rotation speed of the clamping frame is much lower than that of the shaft, thus preventing the clamping frame from loosening and structural breakage due to excessively high rotation speed between the clamping frame and the nucleic acid synthesis column.

[0012] Furthermore, the propeller has three blades, which are arranged in a ring array.

[0013] By adopting the above technical solution, the water flow is stirred up after the blades rotate at high speed, so that the water can be heated evenly, improving the steam production efficiency, and the water flow will generate eddies while being stirred by the blades.

[0014] Furthermore, a heater is installed inside the lower part of the main body of the device, and an inlet and an outlet are respectively connected to the lower sides of the main body of the device.

[0015] By adopting the above technical solution, the staff inputs water into the lower part of the main body of the device through the liquid inlet. Then, the staff turns on the heater to heat the water and vaporize it to produce water vapor. Subsequently, the wastewater generated by ammonia decomposition is discharged through the liquid outlet.

[0016] Furthermore, a sealed door is connected to one side of the top of the device body, the clamping frame is located inside the device body, and a clamping seat is fixed inside the lower part of the clamping frame. A nucleic acid synthesis column is connected inside the clamping frame and the clamping seat.

[0017] By adopting the above technical solution, the staff can open the sealed door and then insert the nucleic acid synthesis column into the clamping frame and clamping seat. The clamping frame and clamping seat will clamp and limit the nucleic acid synthesis column. The staff can also directly rotate the clamping frame by hand to install other nucleic acid synthesis columns. After that, the staff will close the sealed door.

[0018] Furthermore, the sealing door corresponds to the nucleic acid synthesis column, and the nucleic acid synthesis column is detachably connected to the clamping frame and clamping seat.

[0019] By adopting the above technical solution, the staff inserts the nucleic acid synthesis column into the clamping frame and clamping seat, and clamps and limits the nucleic acid synthesis column by the clamping frame and clamping seat.

[0020] Furthermore, a one-way valve is installed on the other side of the top of the device body, and a nozzle is connected to one side of the one-way valve via a pipe.

[0021] By adopting the above technical solution, staff spray acetonitrile into the main body of the device through one-way valves, pipes and nozzles, so that the acetonitrile comes into contact with the nucleic acid synthesis column for purification.

[0022] Furthermore, the outer ring of the rotating shaft is provided with multiple stirring blades, and the multiple stirring blades are distributed in a ring array.

[0023] By adopting the above technical solution, the high-speed rotating stirring blades can make ammonia gas and water vapor mix evenly, thereby improving the ammonia hydrolysis efficiency.

[0024] In summary, the present invention has the following main advantages:

[0025] This invention utilizes a combination of paddles, fan blades, a clamping frame, and push plates. A motor drives both the paddles and fan blades to rotate at high speed simultaneously. The high-speed rotation of the fan blades generates airflow, increasing the contact between ammonia and the nucleic acid synthesis column. The high-speed rotation of the paddles agitates the water flow, ensuring uniform heating and improving steam production efficiency. The agitation of the water flow by the paddles generates eddies, and the arc-shaped push plate provides significant water resistance, allowing these eddies to drive the clamping frame to rotate, which in turn drives the nucleic acid synthesis column, further increasing the contact between ammonia and the column. The high-speed rotation of the paddles creates eddies in the water, which in turn drive the clamping frame. While this process involves significant energy consumption, the limited number of push plates and their small contact area with the water flow ensure that the clamping frame rotates at a much lower speed than the shaft, preventing loosening and structural breakage due to excessively high rotation speeds. This improves the efficiency of the ammonolysis reaction and avoids excessive centrifugal force affecting the nucleic acid synthesis column. Attached Figure Description

[0026] Figure 1 This is a schematic diagram of the structure of this utility model;

[0027] Figure 2 This is a cross-sectional structural diagram of the present invention;

[0028] Figure 3 This is a schematic diagram of the rotating shaft structure of this utility model;

[0029] Figure 4 This is a bottom view of the clamping frame structure of this utility model.

[0030] In the diagram: 1. Main body of the device; 2. Inlet; 3. Outlet; 4. Sealing door; 5. One-way valve; 6. Pipeline; 7. Nozzle; 8. Heater; 9. Motor; 10. Shaft; 11. Paddle; 12. Stirring blade; 13. Fan blade; 14. Clamping frame; 15. Clamping seat; 16. Nucleic acid synthesis column; 17. Push plate. Detailed Implementation

[0031] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0032] The embodiments of this utility model will be described below based on its overall structure.

[0033] Example 1:

[0034] An apparatus for gas-phase ammonolysis of nucleic acids, such as Figures 2-4As shown, the device includes a main body 1 and a clamping frame 14. Four push plates 17 are connected to the bottom of the clamping frame 14, arranged in a circular array. The push plates 17 are arc-shaped. The clamping frame 14 is rotatably connected to the main body 1. When the water is agitated by the paddle 11, eddies are generated. The arc-shaped push plates 17 have significant water resistance, allowing the eddies to drive the clamping frame 14 to rotate, thereby driving the nucleic acid synthesis column 16 to rotate. This further increases the contact between ammonia and the nucleic acid synthesis column 16. The high-speed rotation of the paddle 11 generates eddies in the water, which then drive the clamping frame 14 to rotate. This process involves significant energy consumption, and the relatively small number of push plates 17 results in a small contact area with the water flow. The small size ensures that the rotation speed of the clamping frame 14 is much lower than that of the rotating shaft 10, thus preventing the clamping frame 14 from loosening and structural breakage due to excessively high rotation speed between the clamping frame 14 and the nucleic acid synthesis column 16. A motor 9 is installed on the top of the main body 1 of the device, and the output end of the motor 9 is connected to the rotating shaft 10. The outer surface of the rotating shaft 10 is connected to the paddle 11 and the fan blade 13 respectively. There are three paddle blades 11, which are arranged in a ring array. The motor 9 drives the paddle 11, the stirring blade 12 and the fan blade 13 to rotate at high speed at the same time. The high-speed rotating fan blade 13 generates airflow to increase the contact between ammonia and the nucleic acid synthesis column 16. The high-speed rotation of the paddle 11 agitates the water flow, so that the water can be heated evenly and the water vapor production efficiency can be improved.

[0035] See Figure 1 , Figure 2 and Figure 4 In the above embodiment, a heater 8 is installed inside the lower part of the device body 1. An inlet 2 and an outlet 3 are connected to the lower sides of the device body 1, respectively. Workers input water into the lower part of the device body 1 through the inlet 2, and then turn on the heater 8 to heat the water, causing it to vaporize and produce steam. A sealing door 4 is connected to one side of the top of the device body 1. A clamping frame 14 is located inside the device body 1, and a clamping seat 15 is fixed inside the lower part of the clamping frame 14. A nucleic acid synthesis column 16 is connected inside the clamping frame 14 and the clamping seat 15. The sealing door 4 corresponds to the nucleic acid synthesis column 16, and the nucleic acid synthesis column 16 is connected to the clamping frame 14 and the clamping seat. 15. After disassembling the connection, the staff opens the sealed door 4, and then inserts the nucleic acid synthesis column 16 into the clamping frame 14 and clamping seat 15. The clamping frame 14 and clamping seat 15 clamp and limit the nucleic acid synthesis column 16. The staff can directly rotate the clamping frame 14 by hand to install other nucleic acid synthesis columns 16. After that, the staff closes the sealed door 4. A one-way valve 5 is installed on the other side of the top of the device body 1. A nozzle 7 is connected to one side of the one-way valve 5 through the pipe 6. The staff sprays acetonitrile into the device body 1 through the one-way valve 5, the pipe 6 and the nozzle 7, so that the acetonitrile comes into contact with the nucleic acid synthesis column 16 for purification.

[0036] Example 2:

[0037] Based on the above embodiment one, in order to facilitate the mixing of water vapor and ammonia, the following settings are now adopted.

[0038] See Figure 2 and Figure 3 In the above embodiment, the outer ring of the rotating shaft 10 is provided with a plurality of stirring blades 12, which are arranged in a ring array. The high-speed rotating stirring blades 12 can make ammonia gas and water vapor mix evenly.

[0039] The implementation principle of this utility model is as follows: First, the staff opens the sealing door 4, and then the staff can insert the nucleic acid synthesis column 16 into the clamping frame 14 and the clamping seat 15. The clamping frame 14 and the clamping seat 15 clamp and limit the nucleic acid synthesis column 16. The staff can directly rotate the clamping frame 14 by hand to install other nucleic acid synthesis columns 16. Then the staff closes the sealing door 4.

[0040] The staff inputs water into the lower part of the main body 1 of the device through the liquid inlet 2. Then, the staff turns on the heater 8 to heat the water and vaporize it to produce water vapor. At the same time, the staff introduces ammonia into the main body 1 of the device. The ammonia introduction structure is existing technology and is not shown in the figure. Then, the water vapor combines with the ammonia and comes into contact with the nucleic acid synthesis column 16 to start the ammonolysis process.

[0041] Simultaneously, the operator starts the motor 9, which drives the paddle 11, stirring blade 12, and fan blade 13 to rotate at high speed. The high-speed rotating fan blade 13 generates airflow to increase the contact between ammonia and nucleic acid synthesis column 16. The high-speed rotating paddle 11 agitates the water flow, thereby enabling the water to be heated evenly and improving the steam production efficiency. The high-speed rotating stirring blade 12 can evenly mix ammonia and steam, and the water flow generates eddies while being agitated by the paddle 11. The arc-shaped push plate 17 has a large water resistance, which allows the eddies to drive the clamping frame 14 to rotate, thereby driving the nucleic acid synthesis column 16 to rotate, further increasing the contact between ammonia and nucleic acid synthesis column 16. The high-speed rotation of the paddle 11 generates eddies in the water, which in turn drive the clamping frame 14 to rotate. During this process, there is a large energy consumption. The number of push plates 17 is small and the contact area with the water flow is small, so the rotation speed of the clamping frame 14 is much lower than that of the rotating shaft 10, avoiding the phenomenon of loose clamping and structural breakage due to excessive rotation speed of the clamping frame 14 and nucleic acid synthesis column 16.

[0042] Afterwards, the staff discharged the wastewater generated by ammonolysis through the outlet 3. At the same time, the staff sprayed acetonitrile into the main body 1 of the device through the one-way valve 5, the pipe 6 and the nozzle 7, so that the acetonitrile came into contact with the nucleic acid synthesis column 16 for purification. At the same time, the vortex generated by the rotation of the blade 11 could continue to drive the clamp 14 to rotate, so that the subsequently sprayed acetonitrile could fully come into contact with the nucleic acid synthesis column 16. Then, the staff discharged and recovered the acetonitrile through the outlet 3. Finally, the staff turned off the heater 8 and the motor 9 and opened the sealing door 4 to take out the nucleic acid synthesis column 16.

[0043] Although embodiments of the present invention have been shown and described, these specific embodiments are merely explanations of the present invention and are not intended to limit the invention. The specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. After reading this specification, those skilled in the art may make modifications, substitutions, and variations to the embodiments as needed without departing from the principles and spirit of the present invention, provided that such modifications, substitutions, and variations are within the scope of the claims of the present invention and are protected by patent law.

Claims

1. An apparatus for gas-phase ammonolysis of nucleic acid synthesis, comprising a main body (1) and a clamping frame (14), characterized in that: The bottom of the clamping frame (14) is connected to a push plate (17), the top of the main body (1) of the device is equipped with a motor (9), and the output end of the motor (9) is connected to a rotating shaft (10). The outer surface of the rotating shaft (10) is connected to a blade (11) and a fan blade (13).

2. The apparatus for gas-phase ammonolysis of nucleic acid synthesis according to claim 1, characterized in that: The push plate (17) is arc-shaped, and the clamping frame (14) is rotatably connected to the main body (1) of the device.

3. The apparatus for gas-phase ammonolysis of nucleic acid synthesis according to claim 2, characterized in that: The pusher plate (17) is provided in four parts, and the four pusher plates (17) are distributed in a ring array.

4. The apparatus for gas-phase ammonolysis of nucleic acid synthesis according to claim 1, characterized in that: The blades (11) are provided in three parts, and the three blades (11) are arranged in a ring array.

5. The apparatus for gas-phase ammonolysis of nucleic acid synthesis according to claim 1, characterized in that: A heater (8) is installed inside the lower part of the main body (1) of the device, and an inlet (2) and an outlet (3) are respectively connected to the lower sides of the main body (1).

6. The apparatus for gas-phase ammonolysis of nucleic acid synthesis according to claim 5, characterized in that: A sealing door (4) is connected to one side of the top of the main body (1) of the device. The clamping frame (14) is located inside the main body (1) of the device, and a clamping seat (15) is fixed inside the lower part of the clamping frame (14). A nucleic acid synthesis column (16) is connected inside the clamping frame (14) and the clamping seat (15).

7. The apparatus for gas-phase ammonolysis of nucleic acid synthesis according to claim 6, characterized in that: The sealing door (4) corresponds to the nucleic acid synthesis column (16), and the nucleic acid synthesis column (16) is detachably connected to the clamping frame (14) and the clamping seat (15).

8. The apparatus for gas-phase ammonolysis of nucleic acid synthesis according to claim 6, characterized in that: A one-way valve (5) is installed on the other side of the top of the main body (1) of the device, and a nozzle (7) is connected to one side of the one-way valve (5) through a pipe (6).

9. The apparatus for gas-phase ammonolysis of nucleic acid synthesis according to claim 1, characterized in that: The outer ring of the rotating shaft (10) is provided with multiple stirring blades (12), and the multiple stirring blades (12) are distributed in a ring array.

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