Anti-pollution cryopreservation tube

By incorporating a protective sleeve and outer cap for sealing the cryovials, and combining this with the levitation function of the floating component, the sealing problem of the cryovials during low-temperature storage and thawing is solved, achieving the effects of preventing contamination and rapid thawing.

CN223490981UActive Publication Date: 2025-10-31CHENGDU ANTICEL BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202422945514.6
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-11-29
Publication Date
2025-10-31
Estimated Expiration
2034-11-29

AI Technical Summary

Technical Problem

Existing cryopreservation tubes have poor sealing properties during low-temperature storage and thawing, which allows liquid nitrogen and water to seep in and contaminate the cryopreserved cells.

Method used

The system employs a protective sleeve and outer cover for sealing, combined with a floating component design, to ensure a tight seal at the connection between the tube body and the cover, preventing liquid nitrogen and water from seeping in, and allowing the tube body to suspend in the water for uniform heating during thawing.

Benefits of technology

It improves the sealing of cryovials, prevents contamination, ensures the purity of frozen cells, accelerates thawing, and is flexible and convenient to operate.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model provides an anti-pollution cryopreservation tube, and relates to the technical field of cryopreservation tubes. The anti-pollution cryopreservation tube comprises a tube body, the tube body is movably sleeved with a tube cover, a protective sleeve is arranged on the outer wall of the tube body, the outer wall of the protective sleeve is movably sleeved with a protective outer cover, the top face of the protective sleeve makes contact with the bottom face of the tube cover, and the tube cover is located between the protective outer cover and the protective sleeve. The outer wall of the pipe body is sleeved with a floating piece in a sliding mode, and the floating piece is located below the protective sleeve. The sealing performance of the cryopreservation tube can be improved, and water or liquid nitrogen is prevented from permeating into the cryopreservation tube to pollute cryopreserved cells.
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Description

Technical Field

[0001] This utility model relates to the field of cryopreservation tube technology, and more specifically, to a contamination-resistant cryopreservation tube. Background Technology

[0002] Cryopreservation tubes are commonly used laboratory consumables in biological research and medicine, often used for the low-temperature transport and storage of tissue or cell samples. Cryopreservation tubes are typically made of high-hardness resin, resistant to high temperatures and pressures, and can be repeatedly frozen and thawed.

[0003] Existing cryopreservation tubes consist of a tube body and a cap screwed onto the tube body. The extremely small gap between the tube body and the cap results in poor sealing. During the cryopreservation process in liquid nitrogen, liquid nitrogen can easily seep in through the gap between the tube body and the cap, leading to cell contamination. Furthermore, during the thawing of cell cryopreservation tubes, they are usually heated in a water bath, where water can also easily seep in through the gap between the tube body and the cap, causing cell contamination. Utility Model Content

[0004] The purpose of this invention is to provide a contamination-resistant cryopreservation tube that can improve the sealing performance of the cryopreservation tube and prevent water or liquid nitrogen from seeping into the cryopreservation tube and contaminating the frozen cells.

[0005] This utility model is achieved through the following technical solution:

[0006] A contamination-resistant cryopreservation tube includes a tube body, a tube cap movably fitted onto the tube body, a protective sleeve provided on the outer wall of the tube body, a protective outer cover movably fitted onto the outer wall of the protective sleeve, the top surface of the protective sleeve contacting the bottom surface of the tube cap, the tube cap being located between the protective outer cover and the protective sleeve, and a floating element slidably fitted onto the outer wall of the tube body, the floating element being located below the protective sleeve.

[0007] Furthermore, an annular sealing groove is provided on the outer wall of the tube, and an annular sealing strip is provided on the inner top surface of the tube cover, the annular sealing strip being adapted to and inserted into the annular sealing groove.

[0008] Furthermore, an identification groove is provided on the outer wall of the tube, and an identification piece is provided in the identification groove.

[0009] Furthermore, a protective shell is provided on the outer wall of the tube, which is used to cover the marking groove.

[0010] Furthermore, the protective sleeve is slidably connected to the pipe body, and the protective sleeve is threadedly connected to the protective outer cover.

[0011] Furthermore, the outer diameter of the floating component is larger than the outer diameter of the protective sleeve.

[0012] Furthermore, a water-absorbing ring is provided at one end of the protective sleeve near the pipe cap, and the pipe cap is provided with a groove corresponding to the water-absorbing ring.

[0013] The technical solution of this utility model has at least the following advantages and beneficial effects:

[0014] This invention uses a protective sleeve and a protective outer cap to seal the connection between the tube cap and the tube body, improving the sealing performance between them and preventing water or liquid ammonia from seeping in through the gaps, thus protecting the cryopreserved cells. By incorporating a floatation element, the cryopreservation tube can be vertically suspended in the water during thawing, with the connection between the tube body and the cap above the water surface. This ensures even heating of the tube, accelerating the thawing process, and also prevents water from entering the tube and causing contamination, further enhancing the cryopreservation tube's airtightness. Attached Figure Description

[0015] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0016] Figure 1 A cross-sectional view of the anti-contamination cryopreservation tube provided in Embodiment 1 of this utility model;

[0017] Figure 2 Provided for Embodiment 1 of this utility model Figure 1 A magnified view of a section at point A in the middle;

[0018] Figure 3 Provided for Embodiment 1 of this utility model Figure 1 A schematic diagram of the structure of the anti-contamination cryopreservation tube.

[0019] Icons: 1-pipe body, 2-pipe cap, 3-protective sleeve, 4-protective outer cover, 5-floating component, 6-ring sealing strip, 7-water absorption ring, 8-marking groove, 9-protective shell. Detailed Implementation

[0020] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. The components of the embodiments of this utility model described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0021] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

[0022] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0023] In the description of this utility model, it should be noted that if terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" appear to indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product is in use, they are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model.

[0024] In the description of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0025] Example 1

[0026] like Figure 1-3 As shown, this embodiment provides a contamination-resistant cryopreservation tube, including a tube body 1, a tube cap 2 movably sleeved on the tube body 1, a protective sleeve 3 provided on the outer wall of the tube body 1, a protective outer cover 4 movably sleeved on the outer wall of the protective sleeve 3, the top surface of the protective sleeve 3 contacting the bottom surface of the tube cap 2, the tube cap 2 being located between the protective outer cover 4 and the protective sleeve 3, and a floating element 5 slidably sleeved on the outer wall of the tube body 1, the floating element 5 being located below the protective sleeve 3.

[0027] The cap 2 is threaded to the tube body 1 to seal the tube body 1 and protect the cryopreserved cells inside. The protective sleeve 3 is connected to the protective outer cap 4 to wrap the connection between the cap 2 and the tube body 1, improving the sealing performance of the connection between the tube body 1 and the cap 2, preventing water or liquid ammonia from seeping in from the gaps between the cap 2 and the tube body 1, and protecting the cryopreserved cells.

[0028] During the thawing of the cryopreservation tube, the float 5 allows the tube body 1 to float vertically in the water, while ensuring that the connection between the tube body 1 and the cap 2 is above the water surface. This ensures that the tube body 1 is heated evenly, accelerating the thawing process, and also prevents water from entering the tube body 1 and causing contamination, thus improving the sealing of the cryopreservation tube. Furthermore, there is no need to clamp or fix the tube body 1 during the thawing process, making the operation flexible and convenient.

[0029] In this embodiment, an annular sealing groove is provided on the outer wall of the pipe body 1, and an annular sealing strip 6 is provided on the inner top surface of the pipe cap 2. The annular sealing strip 6 is adapted to and inserted into the annular sealing groove. The width of the annular sealing groove gradually decreases from top to bottom. The cooperation of the annular sealing groove and the annular sealing strip 6 can improve the sealing performance of the pipe cap 2 to the pipe body 1.

[0030] In this embodiment, an identification groove 8 is provided on the outer wall of the tube 1, and an identification plate is provided in the identification groove 8. The identification plate can identify the name of the frozen cells, which can help staff quickly identify different types of frozen cells and prevent confusion between multiple frozen cells.

[0031] In this embodiment, a protective shell 9 is also provided on the outer wall of the tube body 1. The protective shell 9 is used to cover the marking groove 8. One end of the protective shell 9 is hinged to the inner wall of the marking groove 8, and the other end of the protective shell 9 is snapped into the inner wall of the marking groove 8. The protective shell 9 plays a protective role and can prevent the marking piece from falling off or being damaged by external force.

[0032] In this embodiment, the protective sleeve 3 is slidably connected to the pipe body 1, and the protective sleeve 3 is threadedly connected to the protective outer cover 4. This allows for selective installation of the protective sleeve 3 according to freezing and thawing requirements, making it more convenient.

[0033] In this embodiment, the outer diameter of the float 5 is larger than the outer diameter of the protective sleeve 3. During thawing, the float 5 is tightly attached to the protective sleeve 3, ensuring that the connection between the protective cover 4 and the protective sleeve 3 is also above the water surface, preventing water seepage. After thawing, the float 5 is slid to the bottom of the tube 1, so that the bottom surface of the float 5 is on the same horizontal plane as the bottom surface of the tube 1, increasing the contact area between the tube 1 and the platform, and improving the stability of the cryopreservation tube placed on the platform.

[0034] In this embodiment, a water-absorbing ring 7 is provided at one end of the protective sleeve 3 near the pipe cap 2, and the pipe cap 2 is provided with a groove corresponding to the water-absorbing ring 7. The water-absorbing ring 7 can absorb water that seeps into the space between the protective sleeve 3 and the pipe body 1, preventing water from seeping into the pipe body 1.

[0035] When using cryopreservation, place the cryopreserved cells inside the tube body 1, tighten the tube cap 2, attach the protective sleeve 3 to the outer wall of the tube body 1, slide the protective sleeve 3 until it contacts the tube cap 2, so that the absorbent ring 7 is located in the groove, and tighten the protective outer cap 4.

[0036] When thawing, remove tube 1 from liquid nitrogen, attach float 5 to the outer wall of tube 1, adjust the height of float 5 on tube 1 as needed to suspend tube 1 in water, and then heat the water to thaw.

[0037] The above are merely preferred embodiments of this utility model and are not intended to limit the scope of this utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A type of anti-contamination cryopreservation tube, characterized in that: The device includes a tube body, a tube cap movably fitted onto the tube body, a protective sleeve provided on the outer wall of the tube body, a protective outer cover movably fitted onto the outer wall of the protective sleeve, the top surface of the protective sleeve contacting the bottom surface of the tube cap, the tube cap being located between the protective outer cover and the protective sleeve, and a floating component slidably fitted onto the outer wall of the tube body, the floating component being located below the protective sleeve.

2. The anti-contamination cryopreservation tube according to claim 1, characterized in that, The outer wall of the tube is provided with an annular sealing groove, and the inner top surface of the tube cover is provided with an annular sealing strip, which is adapted to be inserted into the annular sealing groove.

3. The anti-contamination cryopreservation tube according to claim 1, characterized in that, The outer wall of the tube is provided with an identification groove, and an identification piece is placed in the identification groove.

4. The anti-contamination cryopreservation tube according to claim 3, characterized in that, A protective shell is also provided on the outer wall of the tube, which is used to cover the marking groove.

5. The anti-contamination cryopreservation tube according to claim 1, characterized in that, The protective sleeve is slidably connected to the pipe body, and the protective sleeve is threadedly connected to the protective outer cover.

6. The anti-contamination cryopreservation tube according to claim 1, characterized in that, The outer diameter of the floating component is larger than the outer diameter of the protective sleeve.

7. The anti-contamination cryopreservation tube according to claim 1, characterized in that, The protective sleeve is provided with a water-absorbing ring at one end near the pipe cap, and the pipe cap is provided with a groove corresponding to the water-absorbing ring.