Fluorescence immunoassay light path box for accurate detection

Abstract

A horizontal light path groove, a vertical light path groove, a light source generation device and a dichroic mirror are arranged in a light path box body, the vertical light path groove is provided with a first lens assembly and a second lens assembly at the two ends of the dichroic mirror respectively, the first lens assembly comprises a first plano-convex lens and a cylindrical mirror, and the second lens assembly comprises a second plano-convex lens and a second plano-convex lens. The cylindrical mirror is arranged on the side, away from the dichroic mirror, of the first plano-convex lens, the cylindrical mirror can focus light into linear light spots, the problem of mutual interference between adjacent lines of the multi-union card is solved, the linear light spots can excite a single line, the numerical value reading accuracy is improved, and the peak curve recognition rate is increased; the second lens assembly comprises a second plano-convex lens and a third plano-convex lens, the third plano-convex lens is arranged on the side, away from the dichroic mirror, of the second plano-convex lens, the third plano-convex lens is additionally arranged, emitted light spots are parallel light, the light intensity and the light ray are evenly distributed through the secondary convex lens, and the numerical value reading accuracy is improved.

Description

Technical Field

[0001] This utility model relates to the field of immunofluorescence detection equipment technology, and in particular to a fluorescence immunoassay optical path box for accurate detection. Background Technology

[0002] The basic principle of immunofluorescence technology is to organically combine the high specificity of antigen-antibody reactions with the sensitive detectability of fluorescence. It uses fluorescent substances as tracers to label antigens or antibodies, creating specific reagents for detecting the corresponding antibodies or antigens. Specifically, a specific light source is used to excite the fluorescent substance in the antigen-antibody conjugate. The excited fluorescent substance emits fluorescence, which can be observed or quantitatively detected by an analyzer to detect and locate the function of a specific substance. Due to its advantages of accuracy, specificity, sensitivity, and speed, it is widely used in the fields of medicine, biology, and pharmacy.

[0003] With the continuous development and advancement of science and technology, more immunofluorescence technologies are being applied to clinical testing and analysis, and more immunofluorescence detection instruments are entering the market. Generally, the core component of an immunofluorescence detection instrument is the optical detection assembly, or simply the optical path. The optical path consists of multiple components, including a light source generator, lenses, filters, and a fluorescence acquisition device for detection. It also includes structural components that connect and fix these components, forming the optical path box. The optical path box of a fluorescence immunoassay analyzer is a device capable of emitting ultraviolet light, converging parallel light, reflecting light, and receiving the emitted light.

[0004] Application No. 202121350213.9 discloses a fluorescence immunoassay optical path box, including a left optical path plate. A rectangular groove is provided at one end of the side wall of the left optical path plate, and a lamp source mechanism is provided on the rectangular groove. A first semi-cylindrical groove is formed on the left optical path plate, and a collimating lens and a first filter are sequentially arranged in the first semi-cylindrical groove. A second semi-cylindrical groove is provided on the side of the left optical path plate opposite to the collimating lens, and the second semi-cylindrical groove is perpendicular to the first semi-cylindrical groove. A dichroic mirror is provided at the connection between the second and first semi-cylindrical grooves. A first plano-convex lens is provided on the second semi-cylindrical groove below the dichroic mirror, and a second filter and a second plano-convex lens are sequentially arranged on the second semi-cylindrical groove above the dichroic mirror. A right optical path plate is provided on the side of the left optical path plate to cooperate with it. The structure is more compact, the internal components are precisely positioned, assembly and debugging are convenient, and the test data is accurate and stable.

[0005] The aforementioned optical path box has advantages such as compact structure, precise positioning, easy assembly and debugging, accurate test data, and good stability. However, the optical path box still has the following defects: 1. The first plano-convex lens converges the parallel light to form a small light spot at the focal point. The light spot at the focal point is projected onto the reagent card with the sample. The light spot here is circular. Commonly used reagent cards are mostly single cards (2 lines). The spacing between adjacent lines on four- or five-part cards is small. The circular light spot will excite two lines at the same time, resulting in numerical deviation and making it difficult to identify the peak curve; 2. The parallel light is projected again onto the second plano-convex lens. The second plano-convex lens converges the light, and the light spot at the focal point is projected onto the PCB board. The corresponding light intensity is calculated and used by the system for calculation and analysis. The aperture can adjust the light spot characteristics from the plano-convex lens to the photoelectric converter to improve the accuracy and stability of the test. The light intensity and light distribution are uneven after the parallel light passes through the second plano-convex lens and the aperture, making it difficult to read the weak signal of the small value card. Utility Model Content

[0006] To address the problems in related technologies, this application discloses a fluorescence immunoassay optical path box for accurate detection, which solves the problem of low detection accuracy of optical path boxes in related technologies.

[0007] To achieve the above objectives, this application provides the following technical solution:

[0008] A precise fluorescence immunoassay optical path box includes a main body with a horizontal optical path groove inside. A vertical optical path groove is connected to the horizontal optical path groove. A light source generator is located at one end of the horizontal optical path groove. A dichroic mirror is tilted at the front end of the light source generator. The dichroic mirror is located at the intersection of the horizontal and vertical optical path grooves. A first lens assembly and a second lens assembly are respectively located at both ends of the dichroic mirror in the vertical optical path groove. A reagent card is located outside the first lens assembly, and a fluorescence sampling device is located outside the second lens assembly. The first lens assembly includes a first plano-convex lens and a cylindrical lens. The cylindrical lens can focus light into a linear light spot.

[0009] As a further aspect of this application:

[0010] The cylindrical mirror is positioned on the side of the first plano-convex lens away from the dichroic mirror.

[0011] As a further aspect of this application:

[0012] The second lens assembly includes a second plano-convex lens and a third plano-convex lens, wherein the third plano-convex lens is disposed on the side of the second plano-convex lens away from the dichroic mirror.

[0013] As a further aspect of this application:

[0014] A collimating lens is provided between the light source generating device and the dichroic mirror on the horizontal optical path groove.

[0015] As a further aspect of this application:

[0016] A filter is provided between the second lens assembly and the dichroic mirror on the vertical optical path groove.

[0017] As a further aspect of this application:

[0018] The optical path box body has a first limiting groove on the outside of the second lens assembly, and a second limiting groove inside the optical path box body.

[0019] As a further aspect of this application:

[0020] The main body of the optical path box is provided with a heat dissipation groove on the outside of the light source generating device.

[0021] As a further aspect of this application:

[0022] The dichroic mirror is tilted at an angle of 45 degrees.

[0023] In summary, the beneficial effects of this application are as follows:

[0024] 1. A precise fluorescence immunoassay optical path box. The main body of the optical path box has a horizontal optical path slot, a vertical optical path slot, and a light source generator. The light source generator has a dichroic mirror tilted at the front end of the light source emission. The vertical optical path slot has a first lens assembly and a second lens assembly at both ends of the dichroic mirror. A reagent card is located outside the first lens assembly, and a fluorescence sampling device is located outside the second lens assembly. The first lens assembly includes a first plano-convex lens and a cylindrical lens. The cylindrical lens is located on the side of the first plano-convex lens away from the dichroic mirror. The cylindrical lens can focus the light into a linear light spot, which solves the problem of mutual interference between adjacent lines of the multi-card. The linear light spot will excite a single line, which improves the accuracy of numerical reading and the peak curve recognition rate.

[0025] 2. The second lens assembly includes a second plano-convex lens and a third plano-convex lens. The third plano-convex lens is located on the side of the second plano-convex lens away from the dichroic mirror. Adding a third plano-convex lens makes the emitted light spot parallel. Through the double convex lens, the light intensity and light distribution are ensured, which solves the problem of weak signals and difficulty in reading small value cards. Without increasing the power of the light source generating device (increasing the power will increase the heat of the light source), it can distinguish weak light signals from blank signals (noise floor) and improve the accuracy of numerical reading.

[0026] 3. A collimating lens is installed on the horizontal optical path groove between the light source generating device and the dichroic mirror. The collimating lens filters and calibrates the near-parallel light from the light source.

[0027] 4. A filter is provided between the second lens assembly and the dichroic mirror on the vertical optical path groove. The filter can filter out stray light and improve the accuracy of numerical detection.

[0028] 5. The main body of the optical path box is equipped with a first limiting groove and a second limiting groove, which ensures the accuracy of the installation position of the internal components of the optical path box, effectively avoids external light, and improves the detection accuracy.

[0029] 6. The main body of the optical path box is equipped with heat dissipation grooves on the outside of the light source generating device, which improves the heat dissipation of the light source generating device. Attached Figure Description

[0030] The accompanying drawings are provided to further understand this application and form part of the specification. They are used together with the embodiments of this application to explain this application and do not constitute a limitation thereof.

[0031] In the attached diagram:

[0032] Figure 1 This is a schematic diagram of the structure of this application.

[0033] Figure label annotations:

[0034] 1. Main body of the optical path box; 2. Horizontal optical path slot; 3. Vertical optical path slot; 4. Light source generating device; 5. Collimating lens; 6. Dichroic mirror; 7. First lens assembly; 8. Second lens assembly; 9. Reagent card; 10. Fluorescence sampling device; 11. Filter; 12. First limiting slot; 13. Second limiting slot; 14. Heat dissipation slot; 71. First plano-convex lens; 72. Cylindrical mirror; 81. Second plano-convex lens; 82. Third plano-convex lens; Detailed Implementation

[0035] Exemplary embodiments will now be described in detail, examples of which are illustrated in the accompanying drawings. When the following description relates to the drawings, unless otherwise indicated, the same numbers in different drawings denote the same or similar elements. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with this application. Rather, they are merely examples of apparatuses and methods consistent with some aspects disclosed in this embodiment as detailed in the appended claims.

[0036] It should be noted that all directional indicators in the embodiments (such as up, down, left, right, front, back, etc.) are only used to explain the relative positional relationship and movement of each component in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indicator will also change accordingly.

[0037] Furthermore, the use of terms such as "first" and "second" in the embodiments is for descriptive purposes only and does not specifically refer to any order or sequence, nor is it intended to limit this application. It is merely to distinguish components or operations described using the same technical terms and should not be construed as indicating or implying their relative importance or implicitly specifying the number of indicated technical features. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the technical solutions of the various embodiments can be combined with each other, but only if they are feasible for those skilled in the art. If a combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed in this application.

[0038] To further understand the content, features, and effects of this application, the following embodiments are provided, and detailed descriptions are given below in conjunction with the accompanying drawings:

[0039] like Figure 1 As shown:

[0040] A fluorescence immunoassay light path box for precise detection, comprising the light path box body 1.

[0041] The main body 1 of the optical path box is provided with a horizontal optical path groove 2, and a vertical optical path groove 3 is connected to the horizontal optical path groove 2. The horizontal optical path groove 2 and the vertical optical path groove 3 intersect perpendicularly, and the optical path groove provides a channel for the light source to run.

[0042] A light source generating device 4 is provided at one end of the horizontal optical path groove 2. The light source generating device 4 includes LED lights or laser lights, etc. Different light source generating devices 4 can be selected according to different substances being detected.

[0043] A dichroic mirror 6 is provided at a 45-degree angle at the front end of the light source emitting device 4. The dichroic mirror 6 is located at the intersection of the horizontal light path slot 2 and the vertical light path slot 3. The vertical light path slot 3 has a first lens assembly 7 and a second lens assembly 8 at both ends of the dichroic mirror 6. A reagent card 9 is provided on the outside of the first lens assembly 7, and a fluorescence sampling device 10 is provided on the outside of the second lens assembly 8.

[0044] The first lens assembly 7 includes a first plano-convex lens 71 and a cylindrical lens 72. The cylindrical lens 72 is disposed on the side of the first plano-convex lens 71 away from the dichroic mirror 6. The cylindrical lens 72 can focus light into a linear light spot.

[0045] The second lens assembly 8 includes a second plano-convex lens 81 and a third plano-convex lens 82, with the third plano-convex lens 82 disposed on the side of the second plano-convex lens 81 away from the dichroic mirror 6.

[0046] In addition, a collimating lens 5 is provided on the horizontal optical path groove 2 between the light source generating device 4 and the dichroic mirror 6.

[0047] A filter 11 is provided on the vertical optical path groove 3 between the second lens assembly 8 and the dichroic mirror 6.

[0048] Preferably, the main body 1 of the optical path box has a first limiting groove 12 on the outside of the second lens assembly 8, and a second limiting groove 13 inside the main body 1 of the optical path box. The first limiting groove 12 can limit the PCB board, and together with the groove of the optical path box, ensures the accurate and consistent position of the light source generating device 4 and the photocell.

[0049] The second limiting groove 13 is located inside the main body 1 of the optical path box. It is I-shaped, which ensures the installation accuracy and avoids external light interference.

[0050] The main body 1 of the optical path box is provided with a heat dissipation groove 14 on the outside of the light source generating device 4 to prevent the light source generating device 4 from overheating and affecting the light source.

[0051] In practical applications:

[0052] The main body 1 of the optical path box is provided with a horizontal optical path slot 2, a vertical optical path slot 3 and a light source generating device 4. The light source generating device 4 has a dichroic mirror 6 tilted at the front end of the light source emission. The vertical optical path slot 3 has a first lens assembly 7 and a second lens assembly 8 at both ends of the dichroic mirror 6. A reagent card 9 is provided on the outside of the first lens assembly 7 and a fluorescence sampling device 10 is provided on the outside of the second lens assembly 8. The first lens assembly 7 includes a first plano-convex lens 71 and a cylindrical lens 72. The cylindrical lens 72 is located on the side of the first plano-convex lens 71 away from the dichroic mirror 6. The cylindrical lens 72 can focus the light into a linear light spot, which solves the problem of mutual interference between adjacent lines of the multi-card. The linear light spot will excite a single line, which improves the accuracy of numerical reading and the peak curve recognition rate.

[0053] The second lens assembly 8 includes a second plano-convex lens 81 and a third plano-convex lens 82. The third plano-convex lens 82 is located on the side of the second plano-convex lens 81 away from the dichroic mirror 6. Adding a third plano-convex lens 82 makes the emitted light spot parallel light. Through the double convex lens, the light intensity and light distribution are ensured, which solves the problem of weak signal and difficulty in reading small value cards. Without increasing the power of the light source generating device 4 (increasing the power will increase the heat of the light source), it can distinguish weak light signals from blank signals (noise floor) and improve the accuracy of numerical reading.

[0054] A collimating lens 5 is provided on the horizontal light path groove 2 between the light source generating device 4 and the dichroic mirror 6. The collimating lens 5 filters and straightens the near-parallel light from the light source.

[0055] A filter 11 is provided on the vertical optical path groove 3 between the second lens assembly 8 and the dichroic mirror 6. The filter 11 can filter out stray light and improve the accuracy of numerical detection.

[0056] The main body 1 of the optical path box is provided with a first limiting groove 12 and a second limiting groove 13, which ensures the accuracy of the installation position of the internal components of the main body 1 of the optical path box, effectively avoids external light, and improves the detection accuracy.

[0057] The main body 1 of the optical path box has a heat dissipation groove 14 on the outside of the light source generating device 4, which improves the heat dissipation of the light source generating device 4.

[0058] Finally, it should be noted that the above disclosure is merely a preferred embodiment of this application and is not intended to limit this application. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application. The scope of this application is limited only by the appended claims.

Claims

1. A fluorescence immunoassay light path box for precise detection, comprising a light path box body (1), wherein a horizontal light path groove (2) is provided inside the light path box body (1), and a vertical light path groove (3) is provided on the horizontal light path groove (2), a light source generating device (4) is provided at one end of the horizontal light path groove (2), a dichroic mirror (6) is provided at an angle at the front end of the light source emitting device (4), the dichroic mirror (6) is located at the intersection of the horizontal light path groove (2) and the vertical light path groove (3), a first lens assembly (7) and a second lens assembly (8) are respectively provided at both ends of the dichroic mirror (6) in the vertical light path groove (3), a reagent card (9) is provided on the outside of the first lens assembly (7), and a fluorescence sampling device (10) is provided on the outside of the second lens assembly (8), characterized in that: The first lens assembly (7) includes a first plano-convex lens (71) and a cylindrical lens (72), wherein the cylindrical lens (72) can focus light into a linear spot.

2. The fluorescence immunoassay optical path box for precise detection according to claim 1, characterized in that: The cylindrical mirror (72) is positioned on the side of the first plano-convex lens (71) away from the dichroic mirror (6).

3. The fluorescence immunoassay optical path box for precise detection according to claim 1, characterized in that: The second lens assembly (8) includes a second plano-convex lens (81) and a third plano-convex lens (82), wherein the third plano-convex lens (82) is disposed on the side of the second plano-convex lens (81) away from the dichroic mirror (6).

4. The fluorescence immunoassay optical path box for precise detection according to claim 1, characterized in that: A collimating lens (5) is provided on the horizontal optical path groove (2) between the light source generating device (4) and the dichroic mirror (6).

5. The fluorescence immunoassay optical path box for precise detection according to claim 1, characterized in that: A filter (11) is provided between the second lens assembly (8) and the dichroic mirror (6) on the vertical optical path groove (3).

6. The fluorescence immunoassay optical path box for precise detection according to claim 1, characterized in that: The optical path box body (1) has a first limiting groove (12) on the outside of the second lens assembly (8), and a second limiting groove (13) is provided inside the optical path box body (1).

7. The fluorescence immunoassay optical path box for precise detection according to claim 1, characterized in that: The main body (1) of the optical path box is provided with a heat dissipation groove (14) on the outside of the light source generating device (4).

8. The fluorescence immunoassay optical path box for precise detection according to claim 1, characterized in that: The dichroic mirror (6) is tilted at an angle of 45 degrees.

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