Intelligent multi-channel fluorescence immunochromatography analyzer

Abstract

The invention provides an intelligent multi-channel fluorescence immunochromatography analyzer, which belongs to the technical field of optical immunochromatography analyzers and comprises a shell, a driving motor and a power transmission component, wherein the driving motor and the power transmission component are used for providing operation power for mechanical components in the shell; the optical module is used for emitting and receiving detection light; a base is arranged in the shell, a plurality of slots used for containing colloidal gold are fixedly mounted above the base, and the slots can be customized according to specific requirements of customers so as to be suitable for different numbers of multi-connected cards or single cards; the optical module comprises a vertical light path module, a transverse light path module and a mounting plate for supporting the vertical light path module and the transverse light path module; the chromatographic analyzer provided by the invention can solve the problems that equipment needs to be readjusted when fluorescence immunoassay detection and colloidal gold detection are carried out at the same time, and time and labor are wasted at present.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of optical immunochromatographic instruments, and particularly relates to an intelligent multi-channel fluorescence immunochromatographic analyzer. BACKGROUND

[0002] Fluorescence immunochromatography and colloidal gold method are two famous immunological technologies, which can be used to detect and calculate antigen-specific antibodies. Because of their different application ranges, their implementation principles are also different.

[0003] Fluorescence immunochromatography is a common antibody detection technology, which uses an immune reaction to detect antigen-specific antibodies. The technology includes steps such as immune reaction, labeling and analysis.

[0004] Colloidal gold method is another common immunological technology, which, compared with fluorescence immunochromatography, has a similar principle of recognizing antigens, but uses colloidal gold instead of fluorescent dyes as a marker to achieve a rapid and simple antibody detection process.

[0005] Fluorescence immunochromatography can quickly and sensitively detect antigen-specific antibodies, while colloidal gold method has higher specificity and sensitivity, and the analysis result is also more accurate. Colloidal gold method is more suitable for qualitative analysis, while fluorescence immunochromatography is more suitable for quantitative analysis.

[0006] Based on the above differences, in the current social production process, there are often situations that require colloidal gold method detection and fluorescence immunochromatography detection of antibodies at the same time. For the above situation, the existing technology usually needs to replace the detection equipment. Replacing the detection equipment needs to re-commission the equipment, and also needs to replace the carrier, which is time-consuming and laborious, and prolongs the detection cycle. SUMMARY

[0007] The intelligent multi-channel fluorescence immunochromatographic analyzer provided by the application can complete colloidal gold method detection and fluorescence immunochromatography detection of antibody carriers at the same time through special optical module and light source adjustment, solving the problem of time-consuming and laborious and prolonging the detection cycle mentioned in the background technology.

[0008] The application is implemented as follows: The application provides an intelligent multi-channel fluorescence immunochromatographic analyzer, which comprises a shell and a driving motor for providing mechanical components in the shell with running power, a power transmission assembly The shell is provided with a base, and a plurality of slots for containing colloidal gold are fixedly installed above the base. The slots can be customized according to specific customer needs and are suitable for different numbers of multi-card or single-card.

[0009] On the basis of the above technical solutions, the intelligent multi-channel fluorescence immunochromatographic analyzer can be further improved as follows: Further, the optical module comprises a vertical light path module, a horizontal light path module, and a mounting plate for supporting the vertical light path module and the horizontal light path module. The vertical light path module comprises a light source for emitting detection light, the light source is plug-in mounted on the mounting plate, and a collimating mirror is fixedly installed in the light emission direction of the light source. The horizontal light path module comprises a two-way mirror for changing the direction of light, the two-way mirror is vertically plug-in installed on the mounting plate at an angle of 45°, a turning mirror is plug-in installed on one side of the two-way mirror, and a filter is plug-in installed on the other side of the two-way mirror; a convex lens one is plug-in installed on the side of the filter away from the turning mirror, a small hole transparent plate is plug-in installed on the side of the convex lens one away from the filter, a receiver is fixedly installed on the side of the small hole transparent plate away from the convex lens one, and a convex lens two is plug-in installed below the turning mirror. The optical module further comprises an upper cover, which is abuttingly installed above the mounting plate, and the upper cover and the mounting plate are mutually embedded.

[0010] The beneficial effects of the above further scheme are as follows: the collimating mirror is arranged to concentrate the light emitted by the light source, ensure accurate light path, and optimize laser beam transmission efficiency; the two-way mirror is arranged to change the X-axis direction transmission of light to Y-axis direction transmission, and optimize the light transmission path; the turning mirror is arranged to change the Y-axis direction transmission of light to Z-axis direction transmission, ensure that the detection light emitted by the mounting plate irradiates on the colloidal gold, and at the same time, the light reflected by the colloidal gold is transmitted to the receiver; the light irradiated on the colloidal gold inside the detection paper is reflected and received by the receiver through the optical module; the filter is arranged to eliminate the useless wavelengths in the reflected light, and avoid affecting the detection result.

[0011] Further, the power transmission assembly comprises a mounting box for mounting the optical module, an optical axis is penetratively installed on the front and back of the mounting box, and both ends of the optical axis are fixedly installed on the base through a support; an extrusion plate is fixedly installed on the back of the mounting box, a power belt is clamped and fixed between the mounting box and the extrusion plate; the driving motor drives the power belt to move, thereby driving the mounting box to slide on the optical axis.

[0012] Further, the convex lens one and the convex lens two are both plano-convex lenses; the convex surface of the convex lens one is close to the filter, and the convex surface of the convex lens two is close to the turning mirror.

[0013] The beneficial effect of the further scheme is that the convex lens two is arranged to converge the light reflected by the colloidal gold detection paper, thereby improving the information collection capability. The convex lens one is arranged to converge the light reflected by the receiver and to convert the light reflected by the colloidal gold detection paper into parallel light, thereby facilitating the reception by the receiver.

[0014] Further, the convex lens one and the convex lens two are both convex lenses, but the height of one side is much greater than that of the other side, and the ratio ranges from 1:10 to 1:5.

[0015] Further, the turning straight mirror is a triangular prism, and the 45° inclined surface of the turning straight mirror is a mirror surface and is not transparent to light.

[0016] Further, the surface of the pinhole transparent plate is black and is frosted.

[0017] The beneficial effect of the further scheme is that the pinhole transparent plate is arranged to further process the light reflected by the colloidal gold detection paper, thereby reducing the light intensity and improving the acceptability of the light; and the frosted surface of the pinhole transparent plate is arranged to shield the scattered light around the pinhole.

[0018] Further, the filter, the convex lens one, the pinhole transparent plate, and the receiver are coaxial, and the center line is parallel to the plane in which the colloidal gold detection paper is located; and the center line of the convex lens two is perpendicular to the plane in which the colloidal gold detection paper is located.

[0019] Further, all the components in the optical module are provided with gaps for disassembly or replacement of the components.

[0020] Further, the upper surface of the shell is further provided with a window for fixing and mounting a display screen, and the display screen is used to display the detection result information and select the detection mode.

[0021] Compared with the prior art, the intelligent multi-channel fluorescence immunoassay analyzer has the following beneficial effects: BRIEF DESCRIPTION OF DRAWINGS

[0022] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiments. It should be understood that the following drawings only show some embodiments of the present application, and therefore should not be regarded as a limitation on the scope, and for those skilled in the art, other related drawings can also be obtained without creative labor.

[0023] Figure 1 It is an overall structure schematic diagram; Figure 2 It is an internal main structure schematic diagram; Figure 3 This is a top view of the main internal structure; Figure 4 This is a schematic diagram of the optical component distribution structure; Figure 5 This is a schematic diagram of the mounting plate structure; Figure 6 This is a schematic diagram of the upper sealing structure; Figure 7 for Figure 3 Enlarged view of point A in the middle.

[0024] The attached diagram lists the components represented by each number as follows: 10. Outer casing; 11. Slot; 12. Base; 20. Drive motor; 30. Power transmission assembly; 31. Power belt; 32. Optical shaft; 33. Mounting box; 34. Extrusion plate; 40. Optical module; 41. Mounting plate; 42. Vertical light path module; 421. Light source; 422. Collimating lens; 43. Horizontal light path module; 431. Dividing lens; 432. Straightening lens; 433. Filter; 434. Convex lens one; 435. Pinhole lens; 436. Receiver; 437. Convex lens two; 44. Top cover. Detailed Implementation

[0025] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0026] Therefore, the following detailed description of the embodiments of the invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the invention without inventive effort are within the scope of protection of the invention. Example

[0027] like Figures 1-7 As shown, the present invention provides an intelligent multi-channel fluorescence immunochromatographic analyzer, including a housing 10 and a drive motor 20 and a power transmission assembly 30 for providing power to the internal mechanical components of the housing 10. The optical module 40 for emitting and receiving detection light is further included; the base 12 is arranged in the shell 10, a plurality of slots 11 for containing colloidal gold are fixedly installed above the base 12, the slots 11 can be customized according to specific needs of customers, and are suitable for different numbers of multi-card or single card.

[0028] Optionally, in the technical scheme, the optical module 40 comprises a vertical light path module 42, a horizontal light path module 43 and a mounting plate 41 for supporting the vertical light path module 42 and the horizontal light path module 43. The vertical light path module 42 comprises a light source 421 for emitting detection light, the light source 421 is plug-in installed on the mounting plate 41, and the light source 421 is fixedly installed with a collimating mirror 422 in the direction of emitting light. The horizontal light path module 43 comprises a two-way mirror 431 for changing the direction of light, the two-way mirror 431 is vertically plug-in installed on the mounting plate 41 and is inclined by 45°, the two-way mirror 431 is plug-in installed with a turning mirror 432 on one side, the two-way mirror 431 is plug-in installed with a filter 433 on the other side, the filter 433 is plug-in installed with a convex lens one 434 on the side away from the turning mirror 432, the convex lens one 434 is plug-in installed with a small hole transparent plate 435 on the side away from the filter 433, the small hole transparent plate 435 is fixedly installed with a receiver 436 on the side away from the convex lens one 434, and the turning mirror 432 is further plug-in installed with a convex lens two 437 below. The optical module 40 further comprises an upper cover 44, the upper cover 44 is abuttingly installed above the mounting plate 41, and the upper cover 44 and the mounting plate 41 are mutually embedded.

[0029] Optionally, in the technical scheme, the power transmission assembly 30 comprises an installation box 33 for containing the optical module 40, the installation box 33 is penetrated with an optical axis 32 in front and back, both ends of the optical axis 32 are fixedly installed on the base 12 through a support, the installation box 33 is fixedly installed with an extrusion plate 34 at the back, the installation box 33 and the extrusion plate 34 are clamped and fixed with a power belt 31, and the driving motor 20 drives the power belt 31 to move so as to drive the installation box 33 to slide on the optical axis 32.

[0030] Optionally, in the technical scheme, the convex lens one 434 and the convex lens two 437 are both plano-convex lenses, the convex surface of the convex lens one 434 is close to the filter 433, and the convex surface of the convex lens two 437 is close to the turning mirror 432.

[0031] Optionally, in the technical scheme, the convex lens one 434 and the convex lens two 437 are both convex lenses, but the height of one side is much greater than that of the other side, and the ratio range is 1:10 to 1:5.

[0032] Optionally, in the above technical solution, the rotating mirror 432 is a triangular prism, and the 45° inclined surface of the rotating mirror 432 is a mirror surface and is not transparent to light.

[0033] Optionally, in the above technical solution, the filter 433, the convex lens one 434, the pinhole transparent plate 435, and the receiver 436 are coaxial, and the center line is parallel to the plane in which the colloidal gold detection paper is located; the center line of the convex lens two 437 is perpendicular to the plane in which the colloidal gold detection paper is located, and the specific mounting manner is referred to the accompanying drawings. Figure 4 .

[0034] Optionally, in the above technical solution, after the light source 421 emits the detection light, for the convenience of understanding, the position of the horizontal light path module 43 is referred to as the X axis, the position of the vertical light path module 42 is referred to as the Y axis, and the positions of the rotating mirror 432, the convex lens two 437, and the colloidal gold below are referred to as the Z axis, and the light path direction is as follows: (1) light source 421→collimating mirror 422→two-way mirror 431→rotating mirror 432→convex lens two 437→colloidal gold; The colloidal gold reflects the light: colloidal gold→convex lens two 437→rotating mirror 432→two-way mirror 431→filter 433→convex lens one 434→pinhole transparent plate 435→receiver 436; (2) light source 421→collimating mirror 422→two-way mirror 431→filter 433→convex lens one 434→pinhole transparent plate 435→receiver 436; The receiver reflects the light: receiver 436→pinhole transparent plate 435→convex lens one 434→filter 433→two-way mirror 431→rotating mirror 432→convex lens two 437→colloidal gold; The colloidal gold reflects the light: colloidal gold→convex lens two 437→rotating mirror 432→two-way mirror 431→filter 433→convex lens one 434→pinhole transparent plate 435→receiver 436.

[0035] Optionally, in the above technical solution, a stepped through hole for mounting the convex lens two 437 is formed in the mounting plate 41, the rotating mirror 432 is inserted and placed in the stepped through hole, the center axis of the convex lens two 437 is perpendicular to one surface of the rotating mirror 432, and the conversion of the light between the Y axis and the Z axis is realized.

[0036] Optionally, in the above technical solution, the surface of the pinhole transparent plate 435 is black and is frosted.

[0037] Optionally, in the above technical solution, gaps for disassembling or replacing the components are reserved between all the components in the optical module 40.

[0038] Optionally, the upper surface of the shell 10 is further provided with a window for fixing a display screen, and the display screen is used for displaying detection result information and selecting a detection mode.

[0039] The preferred embodiments of the present application have been described above with the preferred embodiments, but the present application is not limited to the above examples, and various modifications and changes can be made by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. An intelligent multi-channel fluorescence immunochromatographic analyzer, characterized in that, It includes a housing (10), a drive motor (20), a power transmission assembly (30), and an optical module (40); the drive motor (20) is used to provide operating power for the mechanical components inside the housing (10); the power transmission assembly (30) is connected to the drive motor (20); the optical module (40) is used to emit and receive detection light. It also includes a base (12), which is disposed inside the outer shell (10) and has a plurality of slots (11) for holding reagents fixedly installed on its top. The optical module (40) includes: a mounting plate (41) for carrying other components, a vertical light path module (42), a horizontal light path module (43), and an upper cover (44). The vertical light path module (42) is mounted on the mounting plate (41) and includes a light source (421) for emitting detection light and a collimating lens (422) for collimating the light. The lateral light path module (43) is mounted on the mounting plate (41) and includes a split mirror (431) for changing the direction of light. The upper cover (44) abuts against and fits onto the mounting plate (41).

2. The intelligent multi-channel fluorescence immunochromatographic analyzer according to claim 1, characterized in that, The transverse light path module (43) also includes: a rotating mirror (432), a filter (433), a first convex lens (434), a pinhole plate (435), a receiver (436), and a second convex lens (437). The reversing mirror (432) is inserted and installed on one side of the split mirror (431); the filter (433) is inserted and installed on the other side of the split mirror (431); the first convex lens (434) is inserted and installed on the side of the filter (433) away from the split mirror (431); the pinhole plate (435) is inserted and installed on the side of the first convex lens (434) away from the filter (433); the receiver (436) is fixedly installed on the side of the pinhole plate (435) away from the first convex lens (434); and the second convex lens (437) is inserted and installed below the reversing mirror (432).

3. The intelligent multi-channel fluorescence immunochromatographic analyzer according to claim 2, characterized in that, The filter (433), convex lens one (434), pinhole plate (435) and receiver (436) are coaxially arranged, and their center lines are parallel to the plane of the colloidal gold detection paper to be tested; the center line of the convex lens two (437) is perpendicular to the plane of the colloidal gold detection paper.

4. The intelligent multi-channel fluorescence immunochromatographic analyzer according to claim 3, characterized in that, Both the first convex lens (434) and the second convex lens (437) are plano-convex lenses; wherein the convex surface of the first convex lens (434) faces the filter (433), and the convex surface of the second convex lens (437) faces the rotating mirror (432).

5. The intelligent multi-channel fluorescence immunochromatographic analyzer according to claim 3, characterized in that, The rotating mirror (432) is a triangular prism structure with a 45° mirror bevel for reflecting light.

6. The intelligent multi-channel fluorescence immunochromatographic analyzer according to claim 1, characterized in that, The power transmission assembly (30) includes a power belt (31), an optical axis (32), a mounting box (33), and an extrusion plate (34). The optical axis (32) is fixedly mounted on the base (12) by a bracket; the mounting box (33) is used to hold and mount the optical module (40), and the mounting box (33) is slidably mounted on the optical axis (32) through the front and back; the power belt (31) is connected to the drive motor (20); the extrusion plate (34) is fixed to the back of the mounting box (33) for clamping and fixing the power belt (31) together with the mounting box (33); The drive motor (20) drives the power belt (31) to move, thereby driving the mounting box (33) to slide along the optical axis (32).

7. The intelligent multi-channel fluorescence immunochromatographic analyzer according to claim 3, wherein the surface of the small-hole plate (435) is a black frosted surface.

8. The intelligent multi-channel fluorescence immunochromatographic analyzer according to claim 2 or 3, characterized in that, The optical module (40) has gaps reserved between each optical component for disassembly or replacement.

9. The intelligent multi-channel fluorescence immunochromatographic analyzer according to claim 1, characterized in that, The upper surface of the outer casing (10) is also provided with a window for fixing and installing a display screen, which is used to display test result information and allow users to select test methods.

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