Polymorphism detection kit with amplification function

Abstract

The utility model relates to the field of kits, and discloses a polymorphic detection kit with an amplification function, which comprises a kit body, a cover plate is detachably mounted at the top end of the outer wall of the kit body through a buckle, a rotating door is arranged on the surface of the kit body, an insertion block is elastically connected to the inner wall of the cover plate through a reset spring, and the inner wall of the insertion block is provided with an amplification function. A moving plate is slidably connected to the inner wall of the inclined face of the triangular block, multiple sets of telescopic rods are fixedly connected to the surface of the rotating column, and positioning assemblies are arranged on the outer walls of the telescopic rods. According to the utility model, the pressing rod, the triangular block and the moving plate in the pushing mechanism interact with each other, when a reagent needs to be taken out, the pressing rod is pressed to push a kit in the placing barrel to be pushed outwards from the rotating door, the operation is simple and rapid, and the convenient reagent taking mode greatly shortens the detection preparation time, improves the detection efficiency and reduces the labor intensity of workers. The method is especially suitable for large-scale detection or emergency detection scenes.

Description

Technical Field

[0001] This invention relates to the field of reagent kits, and more particularly to a polymorphism detection kit with amplification function. Background Technology

[0002] Polymorphism detection kits are a method or tool used to detect gene polymorphisms (i.e., variations in genes that exist in different individuals or populations) in biological samples. These kits are commonly used in fields such as genetics, medicine, forensics, and biotechnology.

[0003] In the field of gene testing, the detection of polymorphisms in the TPMT and NUDT15 genes is of paramount importance for precision medicine and drug development. Accurate detection of these gene polymorphisms helps doctors develop personalized treatment plans, improve the effectiveness and safety of drug therapy, and reduce adverse drug reactions.

[0004] Currently, existing polymorphism detection kits have some shortcomings: on the one hand, the structural design of many kits is not conducive to the rapid removal of reagents, which wastes a lot of time and reduces detection efficiency during emergency or large-scale testing; on the other hand, some kits do not fix the reagents securely enough, and the reagents are easily displaced or damaged during transportation and storage due to bumps, affecting the accuracy of the test results. Therefore, a polymorphism detection kit with amplification function is proposed to solve the above problems. Utility Model Content

[0005] To overcome the above shortcomings, this invention provides a polymorphism detection kit with amplification function, which aims to improve the problem in the prior art that it is not conducive to the rapid extraction of reagents, which wastes a lot of time and reduces detection efficiency in emergency or large-scale detection.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a polymorphism detection kit with amplification function, comprising a box body, a cover plate detachably installed on the top of the outer wall of the box body by means of a buckle, a rotating door provided on the surface of the box body, an insert block elastically connected to the inner wall of the cover plate by means of a return spring, a connecting column fixedly connected to the inner wall of the box body, a rotating column rotatably connected at the center of the connecting column, and a pushing mechanism provided on the inner wall of the rotating column;

[0007] The pushing mechanism includes a pressing rod a, which is elastically connected to the inner wall of the box body by a limiting spring. A triangular block is fixedly connected to the outer wall of the bottom end of the pressing rod a, and a moving plate is slidably connected to the inner wall of the inclined surface of the triangular block. Multiple sets of telescopic rods are fixedly connected to the surface of the rotating column, and a positioning component is provided on the outer wall of the telescopic rod.

[0008] As a further description of the above technical solution:

[0009] The positioning component includes a placement bucket, with a pressing rod b slidably connected through the inner wall of the placement bucket. Two sets of hinge rods are hinged to the outer wall of the bottom end of the pressing rod b. A connecting rod is hinged to the end of the hinge rod away from the pressing rod b. A positioning block is fixedly connected to the top of the outer wall of the connecting rod. The pressing rod b is elastically connected to the inner wall of the bottom end of the placement bucket through a connecting spring.

[0010] As a further description of the above technical solution:

[0011] One end of the reset spring is fixedly connected to the inner wall of the cover plate, and the other end of the reset spring is fixedly connected to the outer wall of the bottom end of the insert block. The insert block is slidably connected to the inner wall of the cover plate.

[0012] As a further description of the above technical solution:

[0013] One end of the limiting spring is fixedly connected to the bottom end of the outer wall of the pressing rod a, and the other end of the limiting spring is fixedly connected to the bottom end of the inner wall of the box.

[0014] As a further description of the above technical solution:

[0015] The pressing rod a passes through and is slidably connected to the inner wall of the rotating column, the moving plate is slidably connected to the bottom end of the inner wall of the box, and the outer wall of the moving plate is in contact with the outer wall of the placement bucket.

[0016] As a further description of the above technical solution:

[0017] The surface of the rotating column has multiple sets of slots, the outer wall of the insert block is inserted into the inner wall of the slot, and the rotating column passes through and is rotatably connected to the inner wall of the cover plate.

[0018] As a further description of the above technical solution:

[0019] The bottom end of the outer wall of the placement bucket is in contact with the bottom end of the inner wall of the box, and the end of the telescopic rod away from the rotating column is fixedly connected to the outer wall of the placement bucket.

[0020] As a further description of the above technical solution:

[0021] One end of the connecting spring is fixedly connected to the bottom end of the outer wall of the pressing rod b, and the other end of the connecting spring is fixedly connected to the bottom end of the inner wall of the placement bucket. The connecting rod passes through and is slidably connected to the inner wall of the bottom end of the placement bucket, and the positioning block is slidably connected to the inner wall of the placement bucket.

[0022] This utility model has the following beneficial effects:

[0023] 1. In this utility model, the pressing rod, triangular block and moving plate in the pushing mechanism interact with each other. When it is necessary to take out the reagent, pressing the pressing rod will push the reagent kit in the container outward from the rotating door. The operation is simple and quick. This convenient reagent taking method greatly shortens the test preparation time and improves the test efficiency. It is especially suitable for large-scale test or emergency test scenarios.

[0024] 2. In this utility model, when placing the reagent, pressing the pressing rod can make the positioning blocks move away from each other. After the reagent is placed, the connecting spring will reset the positioning blocks and make them fit tightly against the reagent, preventing the reagent from being damaged or displaced due to shaking or collision during transportation, storage and use. This reliable protection and fixing mechanism ensures the stability and integrity of the reagent and guarantees the accuracy of the test results. Attached Figure Description

[0025] Figure 1 This is a schematic diagram of the overall three-dimensional structure of a polymorphism detection kit with amplification function proposed in this utility model;

[0026] Figure 2 This is a partial cross-sectional view of the cover plate of a polymorphism detection kit with amplification function proposed in this utility model.

[0027] Figure 3 This invention provides a polymorphism detection kit with amplification function. Figure 2 Enlarged structural diagram of section A;

[0028] Figure 4 This is a partial cross-sectional view of the housing of a polymorphism detection kit with amplification function proposed in this utility model.

[0029] Figure 5 This is a partial cross-sectional view of the positioning component of a polymorphism detection kit with amplification function proposed in this utility model.

[0030] Legend:

[0031] 1. Box body; 2. Buckle; 3. Cover plate; 4. Rotating door; 5. Pushing mechanism; 51. Pressing rod a; 52. Limiting spring; 53. Triangular block; 54. Moving plate; 55. Telescopic rod; 6. Rotating column; 7. Return spring; 8. Insert block; 9. Connecting column; 10. Positioning assembly; 101. Placement bucket; 102. Pressing rod b; 103. Hinge rod; 104. Connecting rod; 105. Positioning block; 106. Connecting spring. Detailed Implementation

[0032] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0033] Reference Figure 1 - Figure 3 This utility model provides an embodiment of a polymorphism detection kit with amplification function, comprising a box body 1. A cover plate 3 is detachably installed on the top of the outer wall of the box body 1 via a snap fastener 2. This connection method facilitates opening and closing the box body 1. When the snap fastener 2 is released, the cover plate 3 can be removed from the top of the box body 1, allowing the kit to be placed inside. The snap fastener 2 is existing technology. A rotating door 4 is provided on the surface of the box body 1. An insert block 8 is elastically connected to the inner wall of the cover plate 3 via a return spring 7. One end of the return spring 7 is fixedly connected to the inner wall of the cover plate 3, and the other end is fixedly connected to the outer wall of the bottom end of the insert block 8. The insert block 8 can slide on the inner wall of the cover plate 3 and maintain a certain position under the action of the return spring 7. The position of the insert 8 is automatically reset after it moves. The insert 8 is slidably connected to the inner wall of the cover plate 3. The inner wall of the box body 1 is fixedly connected to the connecting post 9. The center of the connecting post 9 is rotatably connected to the rotating post 6. The position of the rotating post 6 can be supported by the connecting post 9. The surface of the rotating post 6 has multiple sets of empty slots. The outer wall of the insert 8 is inserted into the inner wall of the empty slot. The rotating post 6 is fixed at a specific angle position by the cooperation of the insert 8 and the empty slot, so that multiple sets of placement buckets 101 can rotate and correspond to the position of the rotating door 4, so that they can be pushed outwards and the reagents inside can be taken out. The rotating post 6 is rotatably connected to the inner wall of the cover plate 3.

[0034] Reference Figure 2 - Figure 4The inner wall of the rotating column 6 is provided with a pushing mechanism 5, which includes a pressing rod a51. The pressing rod a51 is elastically connected to the inner wall of the box body 1 by a limiting spring 52. One end of the limiting spring 52 is fixedly connected to the bottom end of the outer wall of the pressing rod a51, and the other end of the limiting spring 52 is fixedly connected to the bottom end of the inner wall of the box body 1. The function of the limiting spring 52 is to automatically reset the position of the pressing rod a51 after it is pressed down. A triangular block 53 is fixedly connected to the outer wall of the bottom end of the pressing rod a51. A moving plate 54 is slidably connected to the inner wall of the inclined surface of the triangular block 53. A protruding rod is provided on the outer side of the moving plate 54, and the protruding rod can only move within the inclined surface of the triangular block 53. Thus, the descent of the triangular block 53 will push the moving plate 54 to move outward. The rise of block 53 causes the moving plate 54 to move inward. The pressing rod a51 passes through and is slidably connected to the inner wall of the rotating column 6. The moving plate 54 is slidably connected to the bottom end of the inner wall of the box 1. The pressing rod a51 passes through the inner wall of the rotating column 6 and can slide therein. The moving plate 54 slides at the bottom end of the inner wall of the box 1 and its outer wall is in contact with the outer wall of the placement bucket 101. When the pressing rod a51 moves up and down, the interaction between the triangular block 53 and the moving plate 54 can push the placement bucket 101 to move, thereby pushing the reagent kit placed inside it outward from the position after the rotating door 4 is opened. The outer wall of the moving plate 54 is in contact with the outer wall of the placement bucket 101. Multiple sets of telescopic rods 55 are fixedly connected to the surface of the rotating column 6. The outer wall of the telescopic rod 55 is provided with a positioning component 10.

[0035] Reference Figure 4 - Figure 5The positioning component 10 includes a placement bucket 101. The bottom end of the outer wall of the placement bucket 101 contacts the bottom end of the inner wall of the box body 1. The end of the telescopic rod 55 away from the rotating column 6 is fixedly connected to the outer wall of the placement bucket 101. When the rotating column 6 rotates, it will synchronously drive the corresponding placement bucket 101 to rotate synchronously through the telescopic rod 55. At the same time, when the placement bucket 101 is contacted by the moving plate 54 and moves outward, the telescopic rod 55 will synchronously extend and retract. A pressing rod b102 is slidably connected through the inner wall of the placement bucket 101. Two sets of hinge rods 103 are hinged to the outer wall of the bottom end of the pressing rod b102. A connecting rod 104 is hinged to the end of the hinge rod 103 away from the pressing rod b102. A positioning block 105 is fixedly connected to the top of the outer wall of the connecting rod 104. When the pressing rod b102 is pressed down, it will synchronously push the two sets of connecting rods 103 through the hinge rod 103. The connecting rod 104 drives the positioning blocks 105 to move away from each other synchronously on the inner wall of the placement container 101, so that the reagent can be placed. The pressing rod b102 is elastically connected to the inner wall of the bottom end of the placement container 101 through the connecting spring 106. One end of the connecting spring 106 is fixedly connected to the bottom end of the outer wall of the pressing rod b102, and the other end of the connecting spring 106 is fixedly connected to the bottom end of the inner wall of the placement container 101. The function of the connecting spring 106 is to press the pressing rod b102 to automatically reset the position of the two sets of positioning blocks 105 after they move away from each other. Thus, the elastic action of the connecting spring 106 allows the positioning blocks 105 to always be in contact with the reagent, thereby clamping the reagent. The connecting rod 104 passes through and slides on the inner wall of the bottom end of the placement container 101, and the positioning blocks 105 slide on the inner wall of the placement container 101.

[0036] Working principle: When using this reagent kit, first, if you need to put in the reagent, open the box 1, release the buckle 2, remove the cover 3, press the pressing rod b102, the pressing rod b102 moves downward, compressing the connecting spring 106, and at the same time, push the two sets of connecting rods 104 outward through the hinge rod 103. The connecting rods 104 drive the positioning block 105 to move away from each other synchronously on the inner wall of the placement container 101. At this time, the reagent can be put into the placement container 101. After releasing the pressing rod b102, the pressing rod b102 returns to its original position under the action of the connecting spring 106. The positioning block 105 returns to the position that fits with the reagent under the elastic action of the connecting spring 106, clamping and fixing the reagent to prevent the reagent from shaking or shifting in the placement container 101, and ensuring the stability of the reagent during the transportation, storage and use of the reagent kit.

[0037] When it is necessary to remove the reagent from the placement container 101, the insert block 8 can be pulled to disengage it from the through hole of the rotating column 6, thereby causing the telescopic rod 55 to rotate. This aligns the placement container 101 containing the reagent to be removed with the rotating door 4. When the insert block 8 is released, it will be inserted into the corresponding slot of the rotating column 6 under the action of the return spring 7, fixing the position of the rotating column 6. At this time, the corresponding placement container 101 is located at the rotating door 4, which facilitates subsequent operations.

[0038] Pressing the pressing lever a51 causes it to move downwards, compressing the limiting spring 52 and simultaneously causing the triangular block 53 to descend. Due to the sliding connection between the inclined surface of the triangular block 53 and the protrusion of the moving plate 54, the descent of the triangular block 53 pushes the moving plate 54 outwards. The movement of the moving plate 54 pushes the placement container 101, which is in contact with it, outwards. At this time, the telescopic rod 55 extends and retracts synchronously, pushing the placement container 101 outwards from the rotating door 4 for easy retrieval of the reagents inside the placement container 101. After releasing the pressing lever a51, it returns to its initial position under the action of the limiting spring 52, and the moving plate 54 also returns to its original position, preparing for the next material pushing operation.

[0039] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A polymorphism detection kit with amplification function, comprising a housing (1), characterized in that: The top of the outer wall of the box (1) is detachably installed with a cover plate (3) via a buckle (2). The surface of the box (1) is provided with a rotating door (4). The inner wall of the cover plate (3) is elastically connected with a plug (8) via a return spring (7). The inner wall of the box (1) is fixedly connected with a connecting column (9). The center of the connecting column (9) is rotatably connected with a rotating column (6). The inner wall of the rotating column (6) is provided with a pushing mechanism (5). The pushing mechanism (5) includes a pressing rod a (51), which is elastically connected to the inner wall of the box (1) by a limiting spring (52). A triangular block (53) is fixedly connected to the outer wall of the bottom end of the pressing rod a (51). A moving plate (54) is slidably connected to the inner wall of the inclined surface of the triangular block (53). Multiple sets of telescopic rods (55) are fixedly connected to the surface of the rotating column (6). A positioning component (10) is provided on the outer wall of the telescopic rod (55).

2. The polymorphism detection kit with amplification function according to claim 1, characterized in that: The positioning component (10) includes a placement bucket (101), and a pressing rod b (102) is slidably connected through the inner wall of the placement bucket (101). Two sets of hinge rods (103) are hinged to the outer wall of the bottom end of the pressing rod b (102). A connecting rod (104) is hinged to the end of the hinge rod (103) away from the pressing rod b (102). A positioning block (105) is fixedly connected to the top of the outer wall of the connecting rod (104). The pressing rod b (102) is elastically connected to the inner wall of the bottom end of the placement bucket (101) by a connecting spring (106).

3. The polymorphism detection kit with amplification function according to claim 1, characterized in that: One end of the reset spring (7) is fixedly connected to the inner wall of the cover plate (3), and the other end of the reset spring (7) is fixedly connected to the outer wall of the bottom end of the insert (8). The insert (8) is slidably connected to the inner wall of the cover plate (3).

4. The polymorphism detection kit with amplification function according to claim 1, characterized in that: One end of the limiting spring (52) is fixedly connected to the bottom end of the outer wall of the pressing rod a (51), and the other end of the limiting spring (52) is fixedly connected to the bottom end of the inner wall of the box (1).

5. The polymorphism detection kit with amplification function according to claim 1, characterized in that: The pressing rod a (51) passes through and is slidably connected to the inner wall of the rotating column (6), the moving plate (54) is slidably connected to the bottom end of the inner wall of the box (1), and the outer wall of the moving plate (54) is in contact with the outer wall of the placement bucket (101).

6. The polymorphism detection kit with amplification function according to claim 1, characterized in that: The surface of the rotating column (6) has multiple sets of slots, the outer wall of the insert (8) is inserted into the inner wall of the slot, and the rotating column (6) is rotatably connected to the inner wall of the cover plate (3).

7. A polymorphism detection kit with amplification function according to claim 2, characterized in that: The bottom end of the outer wall of the placement bucket (101) is in contact with the bottom end of the inner wall of the box body (1), and the end of the telescopic rod (55) away from the rotating column (6) is fixedly connected to the outer wall of the placement bucket (101).

8. A polymorphism detection kit with amplification function according to claim 2, characterized in that: One end of the connecting spring (106) is fixedly connected to the bottom end of the outer wall of the pressing rod b (102), and the other end of the connecting spring (106) is fixedly connected to the bottom end of the inner wall of the placement bucket (101). The connecting rod (104) passes through and is slidably connected to the inner wall of the bottom end of the placement bucket (101). The positioning block (105) is slidably connected to the inner wall of the placement bucket (101).

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