Special cell preserving fluid bottle with impurity filtering function

Abstract

The utility model discloses a special cell preserving fluid bottle with an impurity filtering function. The bottle body of the special bottle is made of the elastic extrudable material, and the pressure-maintaining plugging filter plate is arranged in the bottle body, so that the functions of sealing, pressure maintaining and filtering of liquid are realized. Through a pressure maintaining mode, the cell preservation liquid can smoothly flow out in a filtering mode when the bottle body is simply extruded, and impurities and mucus are removed. According to the technology, the efficient filtering function is creatively integrated into the bottle body design. Through the synergistic effect of the unique bottle body structure, the flip bottle cap and the pressure-maintaining plugging filter plate, sealed storage, stable pressure maintaining and efficient impurity filtration of the cell preservation liquid are realized. The operation steps are simplified, the use cost is reduced, and the integrity and quality of cells are ensured. The technical highlights lie in excellent filtering effect and simplicity and convenience in operation, the filtering structure is simple, and the use method can also be simple and efficient.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of biological sample's preservation and processing technology in biotechnology, especially a cell preservation solution special bottle with impurity filtering function. BACKGROUND

[0002] When cell preservation solution is used to preserve various cell samples (such as cervical mucus, vaginal secretion, sputum, nasopharyngeal secretion), impurities and mucus often exist due to the following reasons:

[0003] 1. Complexity of cell sources: Cell preservation solution is usually used to preserve cells from various sources, such as cervical mucus, vaginal secretion, sputum, and nasopharyngeal secretion. These cell sources may naturally contain some impurities and mucus, so these substances will also be preserved in the cell preservation solution during the preservation process.

[0004] 2. Additives in the preservation solution: Some chemicals are usually added to the cell preservation solution to maintain the activity of the cells. These additives may sometimes react with the cells, producing some precipitates or mucus.

[0005] 3. Cell metabolism: Even in the preservation solution, cells still undergo some degree of metabolic activity, which may lead to the production of some waste, thus forming impurities and mucus in the preservation solution.

[0006] The presence of these impurities and mucus not only threatens the integrity of the cells, but also interferes with the accuracy of the test results, reduces the efficiency of cell separation, and makes it necessary to remove these impurities and mucus. This is crucial for ensuring the health of the cells and the accuracy of the test results.

[0007] At present, the main methods for filtering impurities and mucus in cell preservation solution are conventional filtration and vacuum filtration.

[0008] 1. Conventional filtration method: Prepare the cell culture solution to ensure that there are no obvious suspended solids and impurities. Then, place the filter on a clean container and slowly pour the cell preservation solution into the filter. After the liquid drips out, collect the cells on the filter.

[0009] 2. Vacuum filtration method: Use a vacuum pump or other equipment to quickly pass the cell preservation solution through the filter by negative pressure suction, thereby removing impurities and mucus. This method is faster and more efficient, but requires the use of specialized equipment.

[0010] Referring to the utility model discloses "a cell filtering device that can be fixed on a storage tube" with the application number 202020939052.6, but as shown in its Figure 1 Although the structure of the freeze storage tube cooperates with the filter tube, as shown in its Figure 1 When using, the concave hole of the drainage tube 4 and the convex 6 of the filter tube are buckled together, and the cell group suspension required for filtering is added into the drainage tube 4, after filtering, the device combined by the drainage tube 4 and the filter tube 3 is taken out, then the tube cover 2 of the storage 1 is covered, and then the drainage tube 4 and the filter tube 3 are pulled apart, which is relatively complicated and time-consuming and labor-consuming.

[0011] Therefore, how to realize the filtering of impurities in the cell preservation solution, and make the filtering structure simple and the use method simple and efficient, has become a technical problem to be solved by the utility model. Utility model content

[0012] The utility model solves the technical problem in the prior art, and provides a cell preservation solution special bottle with impurity filtering function to solve the problems of relatively complex structure or relatively complex method for filtering impurities in cells in the background art.

[0013] To solve the above technical problems, the utility model adopts the following technical scheme:

[0014] A cell preservation solution special bottle with impurity filtering function, comprising a bottle body and a flip cap bottle cap matched with the bottle body, the flip cap bottle cap is provided with a liquid outlet hole, and the bottle body is internally provided with a bottle cavity;

[0015] The cell preservation solution special bottle with impurity filtering function further comprises a pressure retaining plugging filter plate;

[0016] The pressure retaining plugging filter plate is positioned between the bottle cavity and the liquid outlet hole;

[0017] The pressure retaining plugging filter plate and the bottle cavity form an extrusion cavity, and the pressure retaining plugging filter plate and the liquid outlet hole form a plugging type pressure retaining filtering cavity;

[0018] The bottle body is made of an elastic extrudable material that can extrude the liquid in the bottle body.

[0019] As a further scheme of the utility model, the elastic extrudable material includes but is not limited to PE polyethylene, PP polypropylene and TPE thermoplastic elastomer.

[0020] As a further scheme of the utility model, the filter plate of the pressure retaining plugging filter plate is equipped with a molybdenum filter screen with a mesh size of 80-100-150.

[0021] As a further scheme of the utility model, the molybdenum filter screen is fixed on the pressure maintaining and plugging filter plate by embedding for realizing the pressure maintaining and plugging function of the pressure maintaining and plugging filter plate.

[0022] As a further scheme of the utility model, the molybdenum filter screen and the pressure maintaining and plugging filter plate are integrated.

[0023] As a further scheme of the utility model, when the bottle body is operated by extrusion, the angle between the center line of the bottle body and the horizontal line is 60 to 120 degrees, preferably 80 to 100 degrees.

[0024] As a further scheme of the utility model, the flip bottle cap further comprises a cap body, an upper cover and a connecting belt, the cap body and the flip are connected through the connecting belt, the liquid outlet hole is arranged on the cap body, and the flip covers the cap body and seals the liquid outlet hole.

[0025] Compared with the prior art, the utility model has the beneficial effects that: the bottle body is made of elastic extrudable material, the bottle body is internally provided with a pressure maintaining and plugging filter plate, realizing the sealing, pressure maintaining and filtering functions of the liquid.

[0026] The additional aspects and advantages of the utility model will be partly given in the following description, partly will become obvious from the following description, or will be understood through the practice of the utility model. BRIEF DESCRIPTION OF DRAWINGS

[0027] In order to more clearly illustrate the technical scheme in the embodiments of the utility model or the prior art, the following will briefly introduce the drawings needed to be used in the embodiment or the prior art description, obviously, the drawings in the following description are only some embodiments of the utility model, and for those skilled in the art, other drawings can also be obtained according to these drawings without paying the creative labor.

[0028] Figure 1 It is the structural schematic diagram of the utility model.

[0029] Figure 2 It is Figure 1 It is the structural schematic diagram under the state of axial section.

[0030] Figure 3 A is enlarged schematic view of Figure 2

[0031] Figure 4 is a structure schematic view of the flip cap bottle cap in the closed state.

[0032] The reference signs and names in the drawings are as follows:

[0033] The bottle body 1, the flip cap bottle cap 2, the liquid outlet hole 3, the bottle cavity 4, the pressure maintaining plugging and filtering plate 5, the extrusion cavity 6, the pressure maintaining filtering cavity 7, the molybdenum filter screen 8, the upper cover 9, the plugging column 10, the connecting belt 11 and the bottle cap body 12. DETAILED DESCRIPTION

[0034] The technical solutions in the embodiments of the present application will be clearly and completely described below. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all the other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the present application.

[0035] Please refer to Figure 1 -4, in the embodiments of the present application, a cell preservation liquid special bottle with impurity filtering function is mainly composed of a bottle body 1 and a flip cap bottle cap 2 matched with the bottle body 1, and the flip cap bottle cap 2 is provided with a liquid outlet hole 3. This design not only facilitates the operation of the user, but also ensures the sealing of the liquid. The bottle body 1 is internally provided with a bottle cavity 4 for containing the cell preservation liquid.

[0036] The cell preservation liquid special bottle with impurity filtering function further comprises a pressure maintaining plugging and filtering plate 5.

[0037] The pressure maintaining plugging and filtering plate 5 is positioned between the bottle cavity 4 and the liquid outlet hole 3.

[0038] The pressure maintaining plugging and filtering plate 5 and the bottle cavity 4 form an extrusion cavity 6, and the pressure maintaining plugging and filtering plate 5 and the liquid outlet hole 3 form a plugging type pressure maintaining filtering cavity 7.

[0039] The bottle body 1 is made of an elastic extrudable material which can extrude the liquid in the bottle body 1.

[0040] The elastic extrudable material includes but is not limited to PE polyethylene, PP polypropylene and TPE thermoplastic elastomer. The selection of this material is to enable the user to easily extrude the liquid in the bottle body 1, and at the same time, the filtering of the pressure maintaining plugging and filtering plate 5 is achieved by being equipped with a molybdenum filter screen 8 with 80-100-150 mesh, so as to achieve the use effect of extrusion filtering while extruding the liquid.

[0041] ​Meanwhile, when PE polyethylene, PP polypropylene or TPE thermoplastic elastomer is selected as the material of the bottle body 1, the following properties can be referred to for selection according to needs.

[0042] 1. Polyethylene (PE):

[0043] Properties: PE has good flexibility, impact resistance and chemical corrosion resistance. PE is relatively light, easy to process and low in cost.

[0044] Extrusion performance: PE has good extrusion performance and can easily discharge the liquid in the bottle body 1 through extrusion.

[0045] 2. Polypropylene (PP):

[0046] Properties: PP has high hardness, rigidity and heat resistance. It also has excellent chemical stability and electrical insulation performance.

[0047] Extrusion performance: Although PP has high hardness, it still has certain extrusion performance and can be used to discharge the liquid in the bottle body 1 through extrusion.

[0048] 3. Thermoplastic elastomer (TPE):

[0049] Properties: TPE combines the elasticity of rubber and the processing performance of plastic. It has good flexibility, impact resistance and chemical corrosion resistance and can be recycled multiple times.

[0050] Extrusion performance: TPE has good extrusion performance and can easily discharge the liquid in the tube through extrusion. At the same time, TPE also has good sealing performance, which can ensure the sealing performance of the bottle body 1 and the flip cap 2.

[0051] The molybdenum filter screen 8 is fixed on the pressure-keeping plugging filter plate 5 in an embedded manner to realize the pressure-keeping plugging filtering function of the pressure-keeping plugging filter plate 5. In another way, the molybdenum filter screen 8 and the pressure-keeping plugging filter plate 5 are integrated, which belongs to the extended implementation mode known to those skilled in the art.

[0052] The bottle body 1 and the flip cap 2 are threadedly connected, which ensures that the bottle body 1 and the flip cap 2 have strong sealing performance, and the pressure-keeping plugging filter plate 5 is positioned between the bottle cavity 4 and the liquid outlet hole 3. The pressure-keeping plugging filter plate 5 can be fixed on the bottle body 1, or fixed on the flip cap 2, or positioned on the bottle body 1 by the thread connection between the bottle body 1 and the flip cap 2, which belongs to the extended connection and fixed installation mode known to those skilled in the art and belongs to the prior art, which will not be described in detail here.

[0053] When the bottle body 1 is operated in an extrusion mode, the center line of the bottle body 1 and the horizontal line need to have an included angle of 60-120 degrees, preferably 80-100 degrees.

[0054] The flip cap bottle cap 2 further comprises a cap body 12, an upper cap 9 and a connecting band 11, the cap body and the flip cap are connected through the connecting band, the liquid outlet hole 3 is arranged on the cap body, and the flip cap seals the liquid outlet hole 3 when the flip cap is covered on the cap body; the upper cap 9 can be provided with a sealing column 10 for sealing the liquid outlet hole 3 of the cap body 12 when the upper cap is covered on the cap body 12; the sealing column 10 and the upper cap 9 are in an integrated structure, so that the upper cap 9 and the cap body 12 have a strong sealing effect, which belongs to the extension embodiment known to those skilled in the art.

[0055] A use method of a cell preservation liquid special bottle with impurity filtering function, the use method comprises the following steps:

[0056] Step 1: open the upper cap 9 of the flip cap bottle cap 2 to expose the liquid outlet hole 3;

[0057] Step 2: tilt the bottle body 1 to align with the target container;

[0058] Step 3: use the atmospheric pressure and the surface tension of water to prevent the liquid from flowing out before extrusion;

[0059] Step 4: extrude the bottle body made of an elastic extrudable material by hand to make the cell preservation liquid pass through the pressure sealing and blocking filter plate 5 between the bottle cavity 4 and the liquid outlet hole 3, and filter out impurities and mucus;

[0060] Step 5: the filtered cell preservation liquid flows into the target container through the liquid outlet hole 3;

[0061] Step 6: after the liquid is poured, the upper cap 9 of the flip cap bottle cap 2 is covered again.

[0062] In step 2, the liquid outlet of the bottle body 1 faces downward, and the center line of the bottle body 1 and the horizontal line need to have an included angle of 60-120 degrees, preferably 80-100 degrees, so that the liquid in the bottle body 1 does not flow downward by using the atmospheric pressure and the surface tension of water, but blocks the pressure sealing and blocking filter plate 5, and the extrusion cavity 6 between the pressure sealing and blocking filter plate 5 and the bottle cavity 4 is also pressure sealed when the bottle body 1 is extruded, so that the gas in the extrusion cavity 6 is not easily discharged, so as to ensure that there is enough gas in the extrusion cavity 6, which is beneficial to filtering out impurities and mucus by extrusion.

[0063] The present application mainly attributes the removal of impurities and mucus to the pore size of the filter membrane and the filtering principle.

[0064] Firstly, the pore size of the filter membrane is carefully designed, using a 80-100-150 mesh molybdenum filter screen 8. This pore size only allows cells to pass through, while blocking impurities and mucus larger than cells. Therefore, when the cell preservation solution passes through the filter membrane, impurities and mucus are effectively intercepted on the filter membrane, thereby achieving the removal of impurities and mucus.

[0065] Secondly, the filtering principle of the filter membrane also plays a key role. The filter membrane usually uses a microporous filtration technology, which uses the difference in pore size to allow small molecular substances such as cells to pass through the membrane pores, while large molecular substances such as impurities and mucus are intercepted. This filtering method not only effectively removes impurities and mucus, but also ensures the integrity and activity of the cells.

[0066] In addition, the filter membrane also has waterproof performance, which can prevent liquid leakage or membrane pore blockage during the filtration process, thereby ensuring the filtration effect and the quality of the cell preservation solution.

[0067] In summary, the cell preservation solution of the present application can remove impurities and mucus by filtering, which is mainly due to the careful design of the pore size and filtering principle of the filter membrane, as well as the waterproof performance of the filter membrane. This method of removing impurities and mucus can ensure the integrity and activity of the cells, improve the separation efficiency, and ensure the purity of the sample, providing reliable data support for subsequent research.

[0068] Example 1

[0069] The set of (cervical) cell preservation solution special bottles is designed based on the principle of air extrusion and water surface tension. The entire working process is as follows: the upper cover of the flip cap bottle cap 2 is popped open with the thumb, exposing the liquid outlet hole 3, maintaining an angle of 60 degrees to 120 degrees between the center line of the bottle body 1 and the horizontal line, and aligning the liquid outlet hole 3 downward to the 1.5ml centrifuge tube. At this time, due to atmospheric pressure and water surface tension, the liquid will not flow out, then the tube is squeezed by hand, the sample in the tube is filtered through the 80-100-150 mesh molybdenum filter screen 8, impurities and mucus are filtered out, and then flows into the 1.5ml centrifuge tube through the liquid outlet hole 3, finally the upper cover of the flip cap bottle cap 2 is flipped, and the entire working process is completed.

[0070] The beneficial effects brought by the present application will be more fully demonstrated in the following specific application scenarios and methods:

[0071] 1. Structural innovation: the present application integrates the unique bottle body 1, flip cap bottle cap 2 and pressure maintaining plugging filter plate 5, ensuring the stability and controllability of the cell preservation solution during the extrusion process, effectively preventing liquid leakage and overflow, and realizing extrusion filtration in a pressure maintaining manner.

[0072] Compared with the case that the traditional filtering method needs to additionally use a filter and a clean container, the bottle body 1 of the utility model integrates the filtering function itself, which greatly simplifies the operation steps and equipment requirements.

[0073] The bottle body 1 is used, and the defined nearly vertical inclination angle is compared with the mode of pouring the liquid in the bottle body 1 in the prior art, and by limiting the different operation mode, the use effect of helping to pressurize and facilitating extrusion filtration is achieved, so as to form a compact and efficient filtration system, realize efficient removal of impurities and mucus, and then realize pressurization sealing by impurities and mucus in the cell sample, and realize the improvement of the filtration effect.

[0074] At the same time, the viewpoint of 'the mode of pouring the liquid in the bottle body 1 in the prior art' in the present application is based on the following reasons:

[0075] a, operation habit: the staff gradually forms a fixed operation habit in long-term practice. In the medical, laboratory and other environments, horizontal pouring of liquid is a common operation mode, and the staff is used to using this mode to handle cell preservation liquid.

[0076] b, control precision: horizontal alignment pouring can ensure that the cell preservation liquid flows out at a stable and uniform speed, which helps to maintain the purity and concentration of the liquid. By horizontal alignment pouring, the staff can more accurately control the flow and direction of the liquid, avoiding waste or spilling.

[0077] c, prevent pollution: the cells and components in the cell preservation liquid are very sensitive to environmental factors. Horizontal alignment pouring can reduce the area and time of liquid contact with air, reducing the risk of pollution. At the same time, this operation mode is also helpful to maintain the vitality and stability of the cells.

[0078] d, safety: the cell preservation liquid usually contains some toxic or harmful components. Horizontal alignment pouring can reduce the possibility of liquid spilling or overflowing during operation, thereby reducing the risk of staff exposure to these harmful substances.

[0079] In summary, when pouring the liquid in the bottle body 1, the staff mainly uses the nearly horizontal mode, or needs to use an additional micropipette to assist the operation.

[0080] 2. High efficiency filtration: The molybdenum filter screen 8 shape adopted by the utility model has excellent filtering effect and larger filtering area, which can quickly and completely remove impurities and mucus in the cell preservation solution.

[0081] Compared with the conventional filtration method, the molybdenum filter screen 8 shape of the utility model can provide higher separation efficiency and purer cell preservation solution, thereby ensuring the accuracy and reliability of subsequent experiments or detection.

[0082] The use of the pressure maintaining and plugging filter plate 5 also realizes the pressure maintaining function in the filtering process, avoids the plugging and the need for pumping equipment that may occur in the conventional vacuum filtration method, and further improves the filtering efficiency and operation convenience.

[0083] 3. Cost saving: By integrating the filtering function in the bottle body 1 itself, the utility model saves the cost of additional purchase of filters and containers in the traditional method, thereby saving the cost for the user.

[0084] The bottle body 1 is made of extrudable material, which not only reduces the manufacturing cost, but also allows the user to easily extrude the liquid without the need for other auxiliary equipment, further reducing the use cost.

[0085] Due to the simple and efficient filtering operation, the utility model reduces the processing time and labor cost, improves the work efficiency, and thus brings economic benefits to the user.

[0086] 4. Environmental sustainability: The utility model adopts a reusable bottle body 1 design, which reduces the use and disposal of traditional filter consumables and reduces the burden on the environment.

[0087] The bottle body 1 is made of extrudable material, which not only reduces the manufacturing cost, but also facilitates the user to perform the extrusion operation and reduces the generation of waste.

[0088] By simplifying the operation steps and equipment requirements, the utility model reduces overall energy consumption and resource waste, meeting the urgent needs of current society for environmental sustainability.

[0089] In summary, the utility model has significant advantages and progress in structural innovation, efficient filtration and environmental sustainability. These features make the utility model have great competitiveness and prospects in the market, and can meet the urgent needs of the biological and pharmaceutical industries and the cell therapy field for high-quality and high-efficiency equipment.

[0090] 5. Simple operation: The utility model realizes a simple and intuitive operation method through unique design and structural innovation. The user only needs a few simple steps to complete the liquid pouring and filtering without complicated operation procedures.

[0091] The inclined angle of the bottle body 1 and the accurate positioning of the pressure retaining plugging filter plate 5 enable the liquid to smoothly flow out through the filter when being squeezed, and further improve the convenience and comfort of operation.

[0092] The flip cap bottle cap 2 design enables the user to easily open and close the bottle cap, realizes convenient liquid pouring and storage, and ensures the sealing and stability of the liquid.

[0093] 6. Wide application: The utility model is suitable for processing and filtering various cell preservation liquids, such as cervical mucus, vaginal secretion, sputum, nasopharyngeal secretion, etc., and has a wide application field.

[0094] Through the efficient filtering system, the utility model can remove impurities and mucus in the cell preservation liquid, ensure the integrity and quality of the cells, and is suitable for clinical detection, scientific research experiment and cell therapy and other fields.

[0095] The flexibility and scalability of the utility model enable it to adapt to cell preservation liquid processing of different specifications and requirements, and meet the needs of different users.

[0096] In summary, the utility model has the advantages of cost saving, simple operation and wide application, and exhibits significant technical effects and progress. These features enable the utility model to have great competitiveness and prospects in the market, and can meet the urgent needs of the biological pharmaceutical industry and the cell therapy field for high-quality and high-efficiency equipment.

[0097] In the utility model, unless otherwise specified and limited, the terms "installation", "setting", "connection", "fixing", "screw connection" and other terms should be understood broadly, for example, it can be fixed connection, or detachable connection, or integrated; it can be mechanical connection, or electrical connection; it can be directly connected, or indirectly connected through an intermediate medium; it can be the communication between two elements or the interaction relationship between two elements, unless otherwise specified, the above-mentioned terms in the utility model can be understood according to the specific meaning of the above-mentioned terms in the utility model by the person skilled in the art.

[0098] It is obvious for those skilled in the art that the utility model is not limited to the details of the above-mentioned exemplary embodiments, and can be realized in other specific forms without departing from the spirit or basic characteristics of the utility model. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting, the scope of the utility model is defined by the appended claims rather than the above-mentioned description, therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the utility model.

Claims

1. A cell preservation solution special bottle with impurity filtering function, comprising a bottle body and a flip cap bottle cap matched with the bottle body, the flip cap bottle cap is provided with a liquid outlet hole, the bottle body is internally provided with a bottle cavity, and characterized in that: The cell preservation solution special bottle with impurity filtering function further comprises a pressure-keeping plugging filter plate; The pressure-keeping plugging filter plate is positioned between the bottle cavity and the liquid outlet hole; The pressure-keeping plugging filter plate and the bottle cavity form a squeezing cavity, and the pressure-keeping plugging filter plate and the liquid outlet hole form a plugging pressure-keeping filtering cavity; The bottle body is made of an elastic squeezable material which can squeeze out the liquid in the bottle body.

2. The cell storage solution dedicated bottle having an impurity filtering function according to claim 1, characterized in that, The elastic squeezable material includes but is not limited to PE, PP and TPE.

3. The cell storage solution dedicated bottle having an impurity filtering function according to claim 1, characterized in that, The filter plate is equipped with a molybdenum filter screen with a mesh size of 80-100-150.

4. The cell storage solution dedicated bottle having an impurity filtering function according to claim 3, characterized in that, The molybdenum filter screen is fixed on the pressure-keeping plugging filter plate by embedding to realize the pressure-keeping plugging filtering function of the pressure-keeping plugging filter plate.

5. The cell storage solution dedicated bottle having an impurity filtering function according to claim 3, characterized in that, The molybdenum filter screen and the pressure-keeping plugging filter plate are in an integrated structure.

6. The cell storage solution dedicated bottle having an impurity filtering function according to claim 3, wherein When the bottle body is operated by squeezing, the center line of the bottle body and the horizontal line need to have an included angle of 60-120 degrees, specifically 80-100 degrees.

7. The cell storage solution dedicated bottle having an impurity filtering function according to claim 1, wherein The flip cap bottle cap further comprises a cap body, a cover and a connecting belt, the cap body and the flip cap are connected by the connecting belt, the liquid outlet hole is arranged on the cap body, and the cover is closed on the cap body, and the flip cap plugs the liquid outlet hole.

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