Blood pretreatment experiment tube

Abstract

The utility model discloses a blood pretreatment experiment tube which comprises a tube body, the internal pressure of the tube body is smaller than the external pressure, and a blood pretreatment reagent is arranged in the tube body. According to the utility model, a blood pretreatment reagent is placed in the blood collection tube, so that the sample treatment flow of red blood cells needing to be lysed is greatly shortened, and the experiment efficiency is improved. A traditional blood pretreatment method needs a plurality of steps and tools, while the blood collection tube can be used for directly carrying out erythrocyte lysis after blood collection, and additional operation is not needed. And secondly, the blood collection tube saves consumables and reduces the experiment cost. And an additional centrifugal tube or lysis solution container is not needed, so that the material consumption in the experiment process is reduced. In addition, according to the blood collection tube, the pollution risk in operation is reduced, and the accuracy and reliability of experiments are improved. The blood and the lysate are mixed and lysed in a closed environment, so that the interference of external pollutants is avoided, and the accuracy of an experimental result is ensured.

Description

TECHNICAL FIELD

[0001] The utility model relates to blood collection tube technical field more specifically, is related to a blood pretreatment experiment tube. BACKGROUND

[0002] In biomedical and clinical research, the lysis of red blood cells is a common blood pretreatment step, mainly used for separating other cellular components in blood, such as white blood cells or platelets, in order to facilitate subsequent cell analysis or extraction of biomolecules within cells. The traditional red blood cell lysis process involves multiple steps and the use of tools. First, a vacuum blood collection tube is used for the collection of blood samples, which is a blood collection method that ensures sterility and contamination, and is widely used in clinical diagnosis and laboratory research. The collected blood sample needs to be transferred to a centrifuge tube containing a red blood cell lysis solution. The red blood cell lysis solution is a special solution that can destroy the red blood cell membrane and release hemoglobin, so that the red blood cells are lysed, and the subsequent separation of cellular components is facilitated.

[0003] In the process of red blood cell lysis, not only the vacuum blood collection tube is needed to ensure the purity and sterility of the blood sample, but also the centrifuge tube and the red blood cell lysis solution are needed to be used together to achieve rapid and effective lysis of red blood cells. Each step of the process needs to be controlled accurately to ensure the accuracy and reliability of the experimental results. However, the process is complex and requires the experimenter to have rich experience and skills.

[0004] The above deficiencies need to be improved. SUMMARY

[0005] In order to solve or alleviate the problem that the existing red blood cell lysis process needs to use multiple tools and the process is complex, the utility model provides a blood pretreatment experiment tube.

[0006] The utility model technical scheme is as follows:

[0007] A blood pretreatment experiment tube, comprising a tube body, the internal pressure of the tube body is less than the external pressure, and a blood pretreatment reagent is arranged in the tube body.

[0008] Further, the blood pretreatment reagent is a red blood cell lysis reagent or a lymphocyte separation reagent.

[0009] Further, the bottom of the tube body is conical, and the diameter gradually decreases from top to bottom.

[0010] Further, the bottom of the tube body is provided with a first sampling port, and the first sampling port is provided with a first rubber plug.

[0011] Further, the pipe body top is provided with a rubber plug, the rubber plug comprises a limiting part and a connecting part arranged below the limiting part, the limiting part is disc-shaped, the diameter of the limiting part is equivalent to the outer diameter of the pipe body, and the connecting part is ring-shaped, and the outer diameter of the connecting part is equivalent to the inner diameter of the pipe body.

[0012] Further, the pipe body top is detachably connected with a pipe cap.

[0013] Further, the pipe body top is provided with external threads, the inner wall of the pipe cap is provided with internal threads, and the pipe body is threadedly connected with the pipe cap.

[0014] Further, a sampling hole is formed in the pipe cap, and a hole cover is detachably connected to the sampling hole.

[0015] Further, a sealing element is arranged between the hole cover and the sampling hole.

[0016] Further, the pipe body is provided with a scale line.

[0017] Further, the pipe body is made of polypropylene or polystyrene.

[0018] The utility model discloses a blood sampling tube, which comprises a pipe body and a pipe cap, and the pipe body is arranged in the pipe cap. BRIEF DESCRIPTION OF DRAWINGS

[0019] In order to more clearly illustrate the technical scheme in the embodiments of the utility model, the drawings needed to be used in the embodiments or prior art description will be briefly introduced as follows. Obviously, the drawings in the following description are only some embodiments of the utility model, and for those skilled in the art, other drawings can also be obtained according to these drawings without creative labor.

[0020] Figure 1 It is a front structure schematic view of the utility model;

[0021] Figure 2 It is a structure explosion drawing of the utility model;

[0022] Figure 3 It is the structure explosion map of the pipe cap in the utility model;

[0023] Figure 4 It is the sectional structure schematic view of the rubber plug in the utility model.

[0024] In the drawing, the reference signs are as follows: 1, pipe body; 101, scale line; 2, blood pretreatment reagent; 3, pipe cap; 301, sampling hole; 302, hole cover; 303, sealing element; 4, rubber plug; 401, limiting portion; 402, connecting portion. DETAILED DESCRIPTION

[0025] In order to make the technical problems, technical solutions and beneficial effects of the utility model clearer and more apparent, the utility model is further described in detail below in combination with the drawings and examples. It should be understood that the specific examples described herein are only used to explain the utility model and are not used to limit the utility model.

[0026] It should be noted that when a component is referred to as being "fixed" or "arranged" or "connected" to another component, it can be directly or indirectly on the other component. The terms "upper", "lower", "left", "right", "front", "back", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like indicate the orientation or position shown in the drawings and are only used for convenience of description and cannot be understood as limiting the technical solutions. The terms "first", "second" and the like are only used for convenience of description and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features. The meaning of "multiple" is two or more, unless otherwise specifically limited. The meaning of "several" is one or more, unless otherwise specifically limited.

[0027] As shown in Figure 1 An embodiment of the utility model discloses a blood pretreatment experimental tube, which comprises a pipe body 1, the internal pressure of the pipe body 1 is less than the external pressure, and a red blood cell lysate is arranged in the pipe body 1.

[0028] In use, first, ensure that the internal pressure of the pipe body 1 is in a negative pressure state, that is, the internal pressure of the pipe body 1 is less than the external pressure, so that when a blood sampling needle is pricked into the vein of a patient, blood can flow into the pipe body 1 under the attraction of the negative pressure without needing an additional power source. Meanwhile, the red blood cell lysate is pre-placed in the pipe body 1, and once the blood flows in, the blood will immediately mix with the lysate and start the process of red blood cell lysis. After the blood mixes with the lysate, the lysis process can be accelerated by simple shaking or rotating operation, so that the red blood cells are completely lysed in a short time. Thereafter, the blood sampling tube can be directly placed into a centrifuge for centrifugation to further separate cell components without needing to be transferred into other containers.

[0029] In this embodiment, by placing the red blood cell lysate in the blood collection tube, the sample processing procedure for lysing red blood cells is greatly shortened, improving the experimental efficiency. Traditional red blood cell lysis methods require multiple steps and tools, while using this blood collection tube can directly perform red blood cell lysis after blood collection without additional operations. Secondly, this blood collection tube saves consumables and reduces experimental costs. Since no additional centrifuge tube or lysate container is needed, material consumption during the experiment is reduced. In addition, this blood collection tube also reduces the risk of contamination during operation, improving the accuracy and reliability of the experiment. Since blood and lysate are mixed and lysed in a closed environment, external contaminants are avoided, ensuring the accuracy of the experimental results.

[0030] In actual application, other blood pretreatment reagents 2 such as lymphocyte separation reagent can also be placed in the tube body 1. The blood pretreatment reagent 2 is a liquid or dry powder.

[0031] As shown in Figure 2 In a preferred example, the bottom of the tube body 1 is conical, with the diameter gradually decreasing from top to bottom. That is, the bottom of the tube body 1 is a pointed bottom.

[0032] In use, due to the conical design of the bottom of the tube body 1, blood will naturally gather towards the bottom during inflow, forming a relatively concentrated blood layer. When blood is mixed with the pre-installed red blood cell lysate in the tube body 1, the conical shape helps the lysate and blood mix more uniformly, ensuring that the red blood cell lysis process is more uniform and efficient. During the mixing process, through simple shaking or rotating operation, blood and lysate can be mixed and reacted more quickly, further promoting the lysis of red blood cells. After mixing is completed, the blood collection tube can be directly placed into a centrifuge to further separate cell components using the centrifugal force of the centrifuge.

[0033] In this embodiment, the pointed bottom blood collection tube allows blood to naturally gather in the tube body 1, facilitating uniform mixing of the red blood cell lysate with the blood, thereby improving the efficiency of red blood cell lysis. Secondly, since the mixing process is more uniform, the lysis effect is also more consistent, which is crucial for subsequent experimental analysis. In addition, the conical pointed bottom design also helps to reduce the residue of blood samples during centrifugation, further improving the utilization of samples, simplifying the experimental process, reducing experimental costs, and improving the accuracy and reliability of the experiment.

[0034] In actual application, after red blood cell lysis, centrifugation is needed to separate cell components, and appropriate blood collection tubes can be selected according to experimental needs.

[0035] If density gradient collection is to be performed, round bottom blood collection tubes are usually chosen. Because of the larger bottom area, round bottom blood collection tubes can withstand greater centrifugal force, ensuring that the sample will not overflow or be damaged during high-speed centrifugation. After red blood cell lysis, different density cell components will be distributed in the blood collection tube according to the density gradient after centrifugation, facilitating subsequent separation and collection.

[0036] When the sample volume is small and the precipitate needs to be collected, a sharp bottom blood collection tube is more suitable. Because the bottom of the sharp bottom blood collection tube is sharp, the precipitate will be more easily concentrated at the bottom, and the upper layer liquid will be more easily sucked with a pipette. After red blood cell lysis, the precipitate will be concentrated at the bottom of the tube after centrifugation, and the supernatant can be easily removed by a pipette.

[0037] The flat bottom blood collection tube is similar to the sharp bottom blood collection tube in use, but the flat bottom design allows the blood collection tube to stand stably on the experimental bench, facilitating placement and removal during the experiment. The flat bottom blood collection tube is also suitable for centrifugal separation after red blood cell lysis, especially in experiments that require frequent manipulation or observation, the use of flat bottom blood collection tubes is more convenient.

[0038] As shown in Figure 2 and Figure 4 , in a preferred example, the top of the tube body 1 is provided with a rubber plug 4, which includes a limiting portion 401 and a connecting portion 402 arranged below the limiting portion 401. The limiting portion 401 is disc-shaped, and the diameter of the limiting portion 401 is comparable to the outer diameter of the tube body 1. The connecting portion 402 is ring-shaped, and the outer diameter of the connecting portion 402 is comparable to the inner diameter of the tube body 1.

[0039] The limiting portion 401 of the rubber plug 4 is disc-shaped, and its diameter is comparable to the outer diameter of the tube body 1, so that when the rubber plug 4 is installed at the top of the tube body 1, the limiting portion 401 can tightly fit with the tube body 1, ensuring the stable installation of the rubber plug. The connecting portion 402 is ring-shaped, and its outer diameter matches the inner diameter of the tube body 1, allowing the connecting portion 402 to be easily inserted into the tube body 1 and tightly fit with the inner wall of the tube body 1, achieving the sealing of the tube body 1. When the tube body 1 needs to be opened, the user can easily remove the rubber plug 4.

[0040] In this embodiment, by providing the rubber plug 4 and stably connecting the rubber plug with the tube body 1, liquid leakage during centrifugation is prevented, ensuring the safety of the experimental process. On the other hand, the rubber plug 4 is relatively soft, which facilitates the piercing of the rubber plug by the blood collection needle, thereby facilitating blood collection. After the needle of the rubber plug is pulled out, the rubber plug 4 can elastically recover, so that the inside of the tube body 1 remains a relatively stable sealed environment, and the blood sample is not easily contaminated. Similarly, after centrifugation, it is also convenient for the needle of the syringe to pierce in, and the supernatant can be easily removed.

[0041] As shown in Figure 1 and Figure 2 , in a preferred example, the top of the tube body 1 is detachably connected with a tube cap 3.

[0042] The pipe body 1 is provided with external threads at the top, and the inner wall of the pipe cap 3 is provided with internal threads, and the pipe body 1 is screwed with the pipe cap 3.

[0043] Since the pipe body 1 is provided with external threads at the top, and the inner wall of the pipe cap 3 is provided with internal threads, the user only needs to align the pipe cap 3 with the top of the pipe body 1, and then rotate the pipe cap 3, so that the internal threads of the pipe cap 3 and the external threads of the pipe body 1 are engaged with each other. With the continuation of the rotation, the pipe cap 3 will gradually move downward until it is completely fixed on the top of the pipe body 1, realizing the close connection of the pipe body 1 and the pipe cap 3. When it is needed to open the pipe body 1, the user only needs to rotate the pipe cap 3 in the opposite direction to separate the internal threads from the external threads, and then the pipe cap 3 can be easily removed.

[0044] In this embodiment, through the mutual engagement of the internal and external threads, a firm connection is formed between the pipe body 1 and the pipe cap 3, effectively preventing the leakage of liquid or gas and ensuring the safety of the experimental process. Secondly, the connection is simple and reliable, and the user only needs to rotate to realize the quick installation and disassembly of the pipe cap 3, greatly improving the work efficiency. In addition, the pipe cap 3 presses the rubber plug 4 against the upper edge of the pipe body 1, improving the sealing performance of the pipe body 1 and further enhancing its stability and reliability in use.

[0045] As shown in Figure 2 and Figure 3 In a preferred example, a sampling hole 301 is formed on the pipe cap 3, and a hole cover 302 is detachably connected to the sampling hole 301.

[0046] A sealing element 303 is arranged between the hole cover 302 and the sampling hole 301. Specifically, the sealing element 303 is a sealing ring.

[0047] When it is needed to extract the upper layer of the pipe body 1, the user directly extracts through the sampling hole 301 without removing the entire pipe cap 3, and the hole cover 302 is detachably connected to the sampling hole 301, and the user only needs to rotate or pull the hole cover 302 to expose the sampling hole 301. Then, the user can insert a straw or other extraction tool into the sampling hole 301 to extract the upper layer. After the extraction is completed, the user rotates or covers the hole cover 302 back to its original position, and the sealing element 303 between the hole cover 302 and the sampling hole 301 realizes sealing, ensuring that the inside of the pipe body 1 is not contaminated by the outside.

[0048] In this embodiment, by opening a sampling hole 301 on the cap 3 and providing a detachable hole cover 302, the convenience of using the tube body 1 is improved, and the sealing performance is significantly improved by providing a sealing member 303. Users can extract the upper layer of the material without removing the entire cap 3, greatly simplifying the operation steps and improving work efficiency. At the same time, the use of the sealing member 303 ensures the tight connection between the hole cover 302 and the sampling hole 301, effectively preventing liquid leakage and ensuring the safety and purity of the material inside the tube body 1.

[0049] As shown in Figure 2 In a preferred example, the tube body 1 is made of transparent material, and the tube body 1 is provided with a scale line 101.

[0050] The scale line 101 is evenly distributed along the length direction of the tube body 1, which is used to indicate the volume or height of the liquid or other material in the tube body 1. Since the tube body 1 is made of transparent material, the user can clearly observe the inside of the tube body 1, and quickly and accurately obtain the volume of the liquid or other material by comparing its height with the scale line 101, so that the user can more conveniently operate and observe in the case of experiments, medical treatment or other occasions requiring accurate measurement of the volume of the material.

[0051] In this embodiment, the transparent tube body 1 and the scale line 101 provided thereon provide the user with an intuitive and convenient measurement experience. The transparent material makes the inside of the tube body 1 clear at a glance, and the user can observe the state and quantity of the material without opening the tube body 1 or using other tools. The scale line 101 improves the accuracy and convenience of measurement, improves work efficiency, reduces operation difficulty and error rate, and ensures the reliability and accuracy of the experiment.

[0052] Specifically, the tube body 1 is made of polypropylene material.

[0053] The tube body 1 made of polypropylene material has good transparency, and the user can clearly observe the inside of the tube body 1, such as the color and clarity of the liquid. At the same time, the corrosion resistance of polypropylene also ensures that the tube body 1 will not be damaged or deformed when storing and transporting various chemicals. It ensures the stability and reliability of the tube body 1 under various experimental conditions, reduces the risk of experimental failure caused by material problems. In addition, the polypropylene material also has good processing performance, which can produce tube bodies 1 of various specifications and shapes to meet the needs of different experimental and medical applications. Finally, the tube body 1 made of polypropylene material has relatively low cost, which is conducive to reducing the cost of experiments and medical treatment.

[0054] In actual application, the pipe body 1 can adopt other materials, which can be selected according to experimental requirements, such as polystyrene. In addition, users can select appropriate specifications of the pipe body 1 according to experimental or medical needs, and the pipe body 1 can be matched with other experimental apparatuses or equipment. Specifically, the pipe body 1 has a diameter of 12 mm and a capacity of 5 ml, and can be suitable for a variety of experimental instruments.

[0055] The above are only preferred embodiments of the present application, and are not used to limit the present application, and any modification, equivalent replacement and improvement made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A blood pre-treatment test tube, characterized by, The application relates to a blood pre-treatment reagent tube, which comprises a tube body, wherein the pressure inside the tube body is less than the pressure outside the tube body, and blood pre-treatment reagents are arranged in the tube body. A rubber plug is arranged at the top of the tube body, wherein the rubber plug comprises a limiting part and a connecting part arranged below the limiting part; the limiting part is disc-shaped, the diameter of the limiting part is equivalent to the outer diameter of the tube body, the connecting part is ring-shaped, and the outer diameter of the connecting part is equivalent to the inner diameter of the tube body. A tube cap is detachably connected to the top of the tube body, a sampling hole is formed in the tube cap, a hole cover is detachably connected to the sampling hole, and a sealing element is arranged between the hole cover and the sampling hole.

2. The blood pre-treatment test tube as claimed in claim 1, wherein, The blood pre-treatment reagents are red blood cell lysis reagents or lymphocyte separation reagents.

3. The blood pre-treatment test tube as claimed in claim 1, wherein, The bottom of the tube body is conical, and the diameter gradually decreases from top to bottom.

4. The blood pre-treatment test tube of claim 1, wherein the blood pre-treatment test tube is a blood pre-treatment test tube for a blood pre-treatment test tube set. An outer thread is arranged at the top of the tube body, an inner thread is arranged on the inner wall of the tube cap, and the tube body is threadedly connected with the tube cap.

5. The blood pre-treatment test tube of claim 1, wherein the blood pre-treatment test tube is a blood pre-treatment test tube for a blood pre-treatment test tube set. A scale line is arranged on the tube body.

6. The blood pretreatment test tube of claim 1, wherein The material of the tube body is polypropylene or polystyrene.

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