Magnetic stirring biological culture reactor

Abstract

The utility model belongs to the technical field of biological culture devices, and particularly relates to a magnetic stirring biological culture reactor. The utility model discloses a magnetic stirring biological culture reactor which at least comprises a culture device and a transmission device, the culture device is provided with a culture cavity, a connecting shaft and at least two stirring paddles, and the connecting shaft is arranged in the culture cavity and on a central shaft; each stirring paddle is in a shape with narrow top and wide bottom, and the top end is rotatably connected with the connecting shaft; the transmission device comprises a rotating disc, a second magnet and a driving motor, and the second magnet is arranged on the rotating disc and rotates along with the rotating disc; the culture cavity and the rotating disc are oppositely arranged in sequence from top to bottom. The stirring paddle is in the shape of the sector with the narrow upper part and the wide lower part, and only the top end of the stirring paddle is rotatably connected with the connecting shaft, so that the shearing force can be effectively reduced, and bubbles can be reduced.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the technical field of biological incubator, concretely relates to a magnetic force stirring biological incubation reactor. BACKGROUND

[0002] In the biological culture dish culture process, in order to avoid the biological sedimentation in the culture dish, often with the aid of stirring mechanism to the microorganism or cell is stirred culture. By controlling the speed and steering of the stirrer, can promote the culture conditions such as nutrient, temperature in the culture dish uniform distribution.

[0003] In the prior art, biological culture dish stirring reactor is based on the magnetic transmission principle operation, utilizes the interaction between magnet material to achieve the stirring effect. This magnetic stirring device mainly relies on magnetic transmission, and the torque and rotational speed of the driving motor are transmitted to the stirring paddle in a non-contact manner, and then the material mixing is realized.

[0004] Although the existing biological magnetic stirrer can ensure that the culture conditions such as nutrient, temperature in the culture dish are uniformly distributed to a large extent, however, the excessive shear force caused by stirring and excessive bubbles are still widespread. Excessive shear force is easy to cause damage to cells, and excessive bubbles may hinder the exchange of gas between culture medium and cells (dissolved oxygen mass transfer), affect normal metabolism of cells, and then lead to problems such as reduced survival rate of cells, slowed down cell reproduction speed. Therefore, the problems of shear force and bubbles limit the application of biological reactor to high-density, high-expression, high-quality continuous culture of mammalian cells. UTILITY MODEL CONTENTS

[0005] In order to solve the above-mentioned problems of prior art, the utility model provides a magnetic stirring biological reactor.

[0006] The technical effects achieved by the utility model are realized by the following technical aspects:

[0007] The utility model provides a magnetic stirring biological incubation reactor, at least including incubator and transmission device;

[0008] The incubator is provided with a culture cavity, a connecting shaft and at least two stirring paddles provided with a first magnet, the connecting shaft is arranged in the culture cavity and on the central shaft, each stirring paddle is in the shape of narrow top and wide bottom, and the top end is rotatably connected with the connecting shaft.

[0009] The transmission device includes a rotating disc, a second magnet for driving the first magnet and a driving motor for driving the rotating disc to rotate, the second magnet is arranged on the rotating disc and rotates with the rotating disc, and the culture cavity and the rotating disc are arranged in sequence from top to bottom.

[0010] In some embodiments, the bottom end of each stirring paddle is provided with a mounting structure for accommodating the first magnet.

[0011] In some embodiments, the culture device and the transmission device are detachably connected.

[0012] In some embodiments, a controller and a control circuit board for outputting a control signal according to an input signal of the controller are further included, the input end of the control circuit board is in communication connection with the controller, and the output end is in communication connection with the driving motor.

[0013] In some embodiments, the rotating disc is provided with a transmission gear; a plurality of rotating discs are arranged in groups, and the transmission gears in each group are driven by a synchronous belt; the driving motor drives one rotating disc in each group, and each rotating disc drives a corresponding stirring paddle.

[0014] In some embodiments, the culture device is further provided with a top cover, the top cover covers the upper opening of the culture cavity, and the connecting shaft is connected with the top cover.

[0015] In some embodiments, a connecting protrusion extending vertically downward is further arranged at the center of the top cover, and the upper end of the connecting shaft is connected with the connecting protrusion.

[0016] In some embodiments, a side wall extending downward is further arranged at the outer ring of the top cover, and a compact part is arranged at the opposite side of the side wall of the culture cavity, and the side wall and the compact part are arranged in cooperation to seal the culture cavity.

[0017] In some embodiments, the transmission device is further provided with a mounting shell, the rotating disc and the driving motor are arranged in the mounting shell, and the culture device is fixedly connected with the upper surface of the mounting shell.

[0018] In some embodiments, a first positioning structure is arranged on the culture device, and a second positioning structure matched with the first positioning structure is arranged on the mounting shell of the transmission device, so that the culture device is movably positioned on the transmission device.

[0019] In summary, the culture device has at least the following advantages:

[0020] The utility model discloses a drive motor drives the rotary disc, and the second magnet on the rotary disc drives the first magnet on the stirring paddle based on magnetic force, to reach the function of driving the stirring paddle. The stirring paddle is in the shape of narrow top and wide bottom, and only the top end is rotatably connected with the connecting shaft, which can effectively reduce the shear force and reduce the generation of bubbles, improve the survival rate of the cells in the suspension culture. In addition, the controller and the control circuit board are used to accurately control the stirring speed and working time of the stirring paddle in the multiple culture devices, which can meet the needs of the small-scale test of multiple parameter comparison at the same time. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 It is the whole structure schematic view of the utility model embodiment 1 or 2;

[0022] Figure 2 It is the explosion schematic view of the whole structure of the utility model embodiment 1 or 2;

[0023] Figure 3 It is the vertical section view of the culture device of the utility model embodiment 1 or 2;

[0024] Figure 4 It is the structure schematic view of the rotary disc and drive motor of the utility model embodiment 1 or 2;

[0025] Figure 5 It is the whole structure schematic view of the utility model embodiment 3;

[0026] Figure 6 It is the explosion schematic view of the whole structure of the utility model embodiment 3;

[0027] Figure 7 It is the structure schematic view of the mounting shell of the utility model embodiment 3 removes the bottom cover.

[0028] Mark in drawing:

[0029] 10, culture device;11, culture cavity;111, compact part;112, first positioning structure;12, connecting shaft;13, stirring paddle;131, mounting structure;14, first magnet;15, top cover;151, connecting protrusion;152, side wall;

[0030] 20, transmission device;21, rotary disc;211, preset slot;212, transmission gear;22, second magnet;23, drive motor;24, mounting shell;241, protective cover;242, fixed frame;243, second positioning structure;25, synchronous belt;

[0031] 30, controller;40, control circuit board. DETAILED DESCRIPTION

[0032] In order to make the purpose, technical scheme and advantages of the embodiments of the present application clearer, the technical scheme of the embodiments of the present application will be described clearly and completely in combination with the drawings in the embodiments of the present application. The described embodiments are part of the embodiments of the present application, but not all the embodiments of the present application.

[0033] Therefore, the following detailed description of the embodiments of the present application provided in the drawings is not intended to limit the scope of the claimed present application, but only represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of protection of the present application.

[0034] Embodiment 1

[0035] Please refer to the accompanying drawings Figure 1 , please combine Figures 2 to 4 The present application provides a magnetic stirring biological culture reactor, at least comprising a culture device 10 and a transmission device 20; the culture device 10 is provided with a culture cavity 11, a connecting shaft 12 and at least two stirring paddles 13 provided with first magnets 14, the connecting shaft 12 is arranged in the culture cavity 11 and on the central axis; each stirring paddle 13 is in the shape of narrow top and wide bottom, and the top end is rotatably connected with the connecting shaft 12; the transmission device 20 comprises a rotating disc 21, a second magnet 22 for driving the first magnet 14 and a driving motor 23 for driving the rotating disc 21 to rotate, the second magnet 22 is arranged on the rotating disc 21 and rotates with the rotating disc 21; the culture cavity 11 and the rotating disc 21 are oppositely arranged in sequence from top to bottom.

[0036] Specifically, the connecting shaft 12 is arranged in the culture cavity 11 and is arranged in the central axis direction of the culture cavity 11. The number of the stirring paddles 13 is at least two leaves, so as to better play the stirring effect. Generally, the stirring paddles 13 are symmetrically and equidistantly arranged with the connecting shaft 12. Each stirring paddle 13 is in the shape of a fan with a narrow upper end and a wide lower end. The top end of each stirring paddle 13 is rotationally connected to the outer surface of the connecting shaft 12 through a ring sleeve. That is, the ring sleeve can rotate around the connecting shaft 12 to stir. The material of the stirring paddle 13 can be selected from, but is not limited to, plastic that can be deformed to a certain extent during rotation and returns to the original shape in the normal state. In the embodiment, two second magnets 22 are arranged on the rotating disc 21 and rotate with the rotating disc 21. The second magnets 22 are mainly used to drive the first magnet 14 by magnetic force during rotation, thereby driving the stirring paddle 13 to rotate. The culture cavity 11, the rotating disc 21 and the driving motor 23 are arranged one by one from top to bottom. The working principle is as follows: the driving motor 23 drives the rotating disc 21 to rotate through mechanical driving. The second magnet 22 on the rotating disc 21 rotates with the rotating disc 21 to drive the first magnet 14 arranged on the stirring paddle by magnetic force, thereby realizing the rotation of each stirring paddle 13 around the connecting shaft 12 in the culture cavity 11 to stir.

[0037] It is worth mentioning that, in the above working principle, since only the top end of the stirring paddle 13 is rotationally connected to the connecting shaft 12, when the stirring paddle 13 rotates around the connecting shaft 12 under the action of the magnetic force, the lower end of the stirring paddle 13 will swing first, and the upper end of the stirring paddle 13 will swing following the lower end. The stirring paddle 13 is in a following rotation state from bottom to top. The rotation state is gentle, that is, the stirring can be started without directly applying shear force to the fluid, which can effectively reduce the shear force. In addition, since the angular velocities of all points of the stirring paddle 13 in the following rotation state are almost the same, according to v = ω·r (v represents linear velocity, ω represents angular velocity, and r represents rotation radius), the closer the stirring paddle in the following state is to the upper end, the smaller the rotation radius of each point of the stirring paddle is, and the closer the linear velocity of each point of the stirring paddle 13 to the top end is. In addition, the liquid close to the water surface is more likely to interact with the external air to form bubbles, and the stirring paddle 13 is in the shape of a fan with a narrow upper end and a wide lower end. The closer the stirring paddle is to the upper end, the smaller the area is. Therefore, the slower the linear velocity is, and the smaller the area is, so that bubbles are less likely to be generated. Thus, the effect of reducing the generation of bubbles can be achieved.

[0038] The unique design of the stirring paddle 13 structure in combination with the driving characteristics between the first magnet 14 and the second magnet 22 can effectively reduce the shear force generated by the stirring paddle 13 on the organism during stirring and reduce the generation of bubbles.

[0039] Embodiment 2:

[0040] This embodiment is a further implementation manner of embodiment 1. Please refer to Figures 2 to 4In the embodiment, the bottom end of each stirring paddle 13 is provided with a mounting structure 131 for accommodating the first magnet 14.

[0041] Specifically, the mounting structure 131 is provided as a groove body matched with the first magnet 14, and the first magnet 14 is arranged in the mounting structure 131. Since the second magnet 22 is arranged below the culture cavity 11, the first magnet 14 arranged at the lower end of the stirring paddle 13 can be better driven by the magnetic force of the second magnet 22. In addition, the lower the first magnet 14 is arranged on the stirring paddle 13, the greater the amplitude of the stirring paddle 13 swings, so that the stirring speed of the upper end edge of the stirring paddle 13 changes more gently, that is, the acceleration of the angular velocity is smaller, so that it is less likely to produce bubbles during stirring.

[0042] In some embodiments, a limiting structure is arranged between each stirring paddle 13 and the connecting shaft 12 to prevent each stirring paddle 13 from being separated from the connecting shaft 12.

[0043] Specifically, the bottom end of the connecting shaft 12 is provided with a limiting structure, which is provided as an annular clamping block to prevent each stirring paddle 13 from being separated from the connecting shaft 12 under the action of gravity.

[0044] In some embodiments, the transmission device 20 is further provided with a mounting shell 24, and the rotating disc 21 and the driving motor 23 are arranged in the mounting shell 24. The culture device 10 is fixedly connected to the upper surface of the mounting shell 24.

[0045] Specifically, the culture device 10 is fixedly connected to the upper surface of the mounting shell 24. At this time, the upper and lower positions of the culture device 10 and the rotating disc 21 are opposite, and the position is unchanged compared with that of the embodiment 1. The mounting shell 24 is arranged in the transmission device 20, which can effectively increase the stability of the whole device.

[0046] In the embodiment, the mounting shell 24 is further provided with a bottom cover for facilitating the disassembly and assembly of the driving motor.

[0047] In some embodiments, the culture device 10 and the transmission device 20 are detachably connected.

[0048] In this way, when the culture device 10 needs to be removed, it can be directly taken off from the transmission device 20, which is more convenient.

[0049] Further, the culture device 10 is provided with a first positioning structure, and the transmission device 20 is provided with a second positioning structure matched with the first positioning structure, so as to movably position the culture device 10 on the transmission device 20.

[0050] Specifically, the mounting shell 24 comprises a protective cover 241, a fixing frame 242, and a second positioning structure 243, the second positioning structure 243 is provided on the protective cover 241, and a first positioning structure 112 is provided at the bottom of the culture cavity 11, the first positioning structure 112 is provided as a groove recessed into the cavity, and the second positioning structure 243 is provided as a protrusion, and the two can be inserted and fixed.

[0051] In addition, a preset groove 211 is provided at the center of the rotating disc 21, the preset groove 211 is recessed downward, and the lower end of the second positioning structure 243 can be inserted and positioned. The rotating disc 21 is arranged between the protective cover 241 and the fixing frame 242, and the driving shaft of the driving motor 23 is drivingly connected with the rotating disc 21 through the fixing frame 242.

[0052] It is worth mentioning that the second positioning structure 243 only plays a role of positioning and pre-fixing for the rotating disc 21, and does not affect the rotating state of the rotating disc 21, and the rotating disc 21 and the culture cavity 11 can be more conveniently arranged in an up-down relative manner, that is, only by inserting the culture cavity 11 into the specified position, the position of the maximum magnetic force can be found.

[0053] In some embodiments, the culture device 10 is further provided with a top cover 15, the top cover 15 is arranged on the upper opening of the culture cavity 11, and the connecting shaft 12 is connected with the top cover 15.

[0054] Specifically, the top cover 15 can conveniently add culture substances at any time, and can effectively isolate the culture cavity 11 from the external environment. The top cover 15 is arranged on the upper opening of the culture cavity 11, the upper end of the connecting shaft 12 is connected with the center of the top cover 15, and the lower end is connected with the top end of each stirring paddle 13. At this time, when the top cover 15 is lifted, the connecting shaft 12 and the stirring paddle 13 can be lifted together, which is more convenient, and does not need to be disassembled and assembled, and is not easy to lose.

[0055] In some embodiments, a connecting protrusion 151 extending vertically downward is further arranged at the center of the top cover 15, and the upper end of the connecting shaft 12 is connected with the connecting protrusion 151.

[0056] Specifically, the upper end of the connecting shaft 12 is connected with the connecting protrusion 151. The connecting protrusion 151 can be connected with the connecting shaft 12 in various ways, such as threaded connection, buckle connection.

[0057] In some embodiments, the outer ring of the top cover 15 is further provided with a side wall 152 extending downward, and the outer wall of the culture cavity 11 is provided with a compact part 111 at the position opposite to the side wall 152 of the top cover 15, and the side wall 152 and the compact part 111 are arranged in cooperation to seal the culture cavity 11.

[0058] In this way, the cooperation between the side wall 152 and the compact part 111 can better achieve the effect of sealing the culture cavity 11.

[0059] The further optimization of the culture device 10 and the transmission device 20 can improve the stability and convenience of the overall device.

[0060] Embodiment 3:

[0061] The embodiment is a further implementation of the embodiment 1 or 2, please refer to Figures 5 to 7 In the embodiment, the magnetic stirring bioreactor further comprises a controller 30 and a control circuit board 40 for outputting a control signal according to the input signal of the controller 30, the input end of the control circuit board 40 is in communication connection with the controller 30, and the output end is in communication connection with the driving motor 23.

[0062] Specifically, the input end of the control circuit board 40 is in communication connection with the controller 30, and the output end is in communication connection with the driving motor 23. It is worth mentioning that the communication connection can be a cable connection, such as through a cable interface on the installation shell 24, so as to realize the cable connection. The controller 30 is mainly used for outputting a control signal to adjust and control the rotation state of the driving motor 23, wherein the controller 30 can be selected but not limited to Bluetooth and the like. The control circuit board 40 mainly converts the output control signal to the driving motor 23 according to the input signal of the controller 30. The driving motor 23 can be self-powered or can be provided with an external power supply.

[0063] In some embodiments, the rotating disc 21 is provided with a transmission gear 212; the rotating disc 21 is arranged in several groups, and the transmission gears 212 in each group are driven by a synchronous belt 25; the driving motor 23 drives one rotating disc 21 in each group, and each rotating disc 21 drives the corresponding stirring paddle 13.

[0064] Specifically, two are arranged in each group in the figure, and each rotating disc 21 drives one stirring paddle 13 in each culture device 10. The rotating discs 21 in each group are connected in transmission through the synchronous belt 25 on the transmission gear 212, and each group is provided with one driving motor 23, that is, one driving motor 23 drives one rotating disc 21 in each group, and the rotating disc 21 drives other rotating discs 21 in the group through the synchronous belt 25. In this way, by ensuring that the rotating discs 21 in the same group have the same rotating speed, the effect of contrast experiment can be achieved, that is, because the rotating speed of each rotating disc 21 in the same group is the same, the experiment can exclude the influence of rotating speed, and energy can also be effectively saved.

[0065] As above, a plurality of culture devices 10 can be arranged, so as to meet the experimental requirements of different numbers of culture devices 10 for different small-scale experiments.

[0066] In the embodiment, the controller 30 transmits control signals to the control circuit board 40 through a single cable, and the control circuit board 40 divides the control signals into three paths to control the three driving motors 23, so as to realize the function of controlling the rotating speed of the driving motor 23 according to the requirement. The controller 30 and the control circuit board 40 can also control the acceleration and other rotating data of the driving motor 23 according to different control strategies. Different accelerations can generate different sizes of shearing force and bubbles on the stirring paddle 13. Generally, the smaller the acceleration is, the smaller the shearing force is, and the fewer the bubbles are.

[0067] In other embodiments, the controller 30 can be a single-chip microcomputer, and the control circuit board 40 can be a PCB board.

[0068] In the utility model, unless another definite provision and limitation, the terms "mount", "connect", "joint", "fix" and so on should do broad sense understanding, for example, can be fixed connection, also can be detachable connection, or be integrated;Can be mechanical connection, also can be electrical connection;Can be direct connection, also can through the indirect connection of intermediate medium, can be the communication of two elements or the interaction of two elements. For ordinary skilled in the art, the above terms can be understood according to the specific meaning of the utility model.

[0069] In the description of the utility model, it needs to be explained that the orientation or position relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and so on is based on the orientation or position relationship shown in the drawing, or the orientation or position relationship of the utility model product when it is usually placed, which is only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, so it cannot be understood as a limitation on the utility model. In addition, the terms "first", "second", "third" and so on are only used for distinguishing description, and cannot be understood as indicating or implying relative importance.

[0070] In addition, the terms "horizontal", "vertical", "overhang" and so on do not mean that the component must be absolutely horizontal or overhanging, but can be slightly inclined. For example, "horizontal" only means that its direction is more horizontal relative to "vertical", and does not mean that the structure must be completely horizontal, but can be slightly inclined.

[0071] In the utility model, unless another definite provision and limitation, first feature is above or below second feature can include that first and second features are in direct contact, also can include that first and second features are not in direct contact but contact through additional feature between them.

[0072] Although the description of the utility model is combined with above specific embodiment, it is obvious that many substitutions, modifications and changes can be made according to the above-mentioned content by the person skilled in the art. Therefore, all such substitutions, improvements and changes are included in the spirit and scope of the appended claims.

Claims

1. A magnetic stirred bioreactor characterized in that, At least comprising a culture device (10) and a transmission device (20); The culture device (10) is provided with a culture cavity (11), a connecting shaft (12) and at least two stirring paddles (13) provided with first magnets (14), the connecting shaft (12) is arranged in the culture cavity (11) and on the central axis; each of the stirring paddles (13) is in the shape of narrow top and wide bottom, and the top end is rotatably connected with the connecting shaft (12); The transmission device (20) comprises a rotating disc (21), a second magnet (22) for driving the first magnet (14) and a driving motor (23) for driving the rotating disc (21) to rotate, the second magnet (22) is arranged on the rotating disc (21) and rotates with the rotating disc (21); the culture cavity (11) and the rotating disc (21) are arranged in sequence from top to bottom.

2. The magnetic stirred bioreactor according to claim 1, characterized in that The bottom end of each of the stirring paddles (13) is provided with a mounting structure (131) for accommodating the first magnet (14).

3. The magnetic stirred bioreactor of claim 1, wherein, The culture device (10) and the transmission device (20) are detachably connected.

4. The magnetic stirred bioreactor of claim 1, wherein, Further comprising a controller (30) and a control circuit board (40) for outputting a control signal according to an input signal of the controller (30), the input end of the control circuit board (40) is in communication connection with the controller (30), and the output end is in communication connection with the driving motor (23).

5. The magnetic stirred bioreactor according to claim 4, characterized in that The rotating disc (21) is provided with a transmission gear (212); the rotating disc (21) is arranged in groups, the transmission gears (212) in each group are driven by a synchronous belt (25); the driving motor (23) drives one of the rotating discs (21) in a group, and each of the rotating discs (21) drives the corresponding stirring paddle (13).

6. The magnetic stirred bioreactor of claim 1, wherein, The culture device (10) is further provided with a top cover (15), the top cover (15) is arranged on the upper opening of the culture cavity (11); the connecting shaft (12) is connected with the top cover (15).

7. The magnetic stirred bioreactor according to claim 6, characterized in that The center of the top cover (15) is further provided with a connecting protrusion (151) extending vertically downward, and the upper end of the connecting shaft (12) is connected with the connecting protrusion (151).

8. The magnetic stirred bioreactor of claim 6, wherein, The outer ring of the top cover (15) is further provided with a side wall (152) extending downward, and the outer wall of the culture cavity (11) is provided with a compact part (111) at the position opposite to the side wall (152) of the top cover (15), and the side wall (152) and the compact part (111) are arranged in cooperation to seal the culture cavity (11).

9. The magnetic stirred bioreactor of claim 1, wherein, The transmission device (20) is further provided with a mounting shell (24), the rotating disc (21) and the driving motor (23) are arranged in the mounting shell (24), and the culture device (10) is fixedly connected with the upper surface of the mounting shell (24).

10. The magnetic stirred bioreactor of claim 9, wherein, The culture device (10) is provided with a first positioning structure (112), and the mounting shell (24) of the transmission device (20) is provided with a second positioning structure (243) matched with the first positioning structure, so as to movably position the culture device (10) on the transmission device (20).

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