Device for dynamically observing stomata motion of living plant leaves

Abstract

The utility model relates to plant physiology research equipment, in particular to a living plant leaf stomata motion dynamic observation device which comprises a microscope, an observation platform and an LED illumination device, the observation platform is placed on the microscope, a plant leaf is clamped and fixed on the observation platform, and the LED illumination device provides a light source for the plant leaf; the induction mechanism is composed of a carbon dioxide concentration control device, a moisture content control device and an abscisic acid release device, and the carbon dioxide concentration control device, the moisture content control device and the abscisic acid release device are connected into the display device and then conveyed to the observation platform. The device can accurately control the carbon dioxide concentration, the moisture content and the release of plant hormones so as to simulate the change of stomatal movement under different environmental conditions, and the LED illumination device is used for adjusting the illumination condition, so that the opening and closing of the stomatal are further controlled; the device can provide real-time experimental data for the fields of plant physiology research, plant breeding and the like, and has the characteristics of multi-factor regulation and control, real-time monitoring, high-precision control and wide application.

Description

TECHNICAL FIELD

[0001] The utility model relates to plant physiology research equipment, concretely relates to a device of live plant leaf stomata movement dynamic observation. BACKGROUND

[0002] Plant stomata movement is the key process of plant gas exchange and water regulation. With the deepening of plant physiology and plant ecology research, observing the dynamic change of stomata movement is of great significance to the study of plant response mechanism to environmental stress. At present, there are some observation devices for stomata movement, but most of the devices cannot accurately control the environmental parameters (such as CO2 concentration, water content, hormone treatment, etc.), and lack the real-time observation ability of stomata movement under the combined action of multiple factors. Therefore, it is urgent to develop a device that can accurately control environmental variables and observe the change of stomata movement in real time. UTILITY MODEL CONTENT

[0003] To solve the above technical problems, the utility model provides a device for dynamic observation of live plant leaf stomata movement, which comprises a microscope, an observation platform and an LED lighting device. The observation platform is placed on the microscope, and the plant leaf is clamped and fixed on the observation platform. The LED lighting device provides light source for the plant leaf.

[0004] During the observation of the plant leaf, the surrounding environment is supplied with external filtered air. The air passes through the carbon dioxide removal device and the water removal device in turn to obtain oxygen / nitrogen mixed gas. The mixed gas is transported into the plant leaf environment after passing through the display device. The mixed gas passes through the display device again after being affected by the plant leaf.

[0005] During the observation of the plant leaf, an induction mechanism is also provided, which comprises a carbon dioxide concentration control device, a water content control device and an abscisic acid release device. The induction mechanism is connected to the display device and transported to the observation platform.

[0006] Preferably, the carbon dioxide removal device is provided with sodium hydroxide and sodium carbonate, and the water removal device is provided with a water absorbent.

[0007] Preferably, the carbon dioxide concentration control device is an adjustable gas supply system, which regulates the concentration of carbon dioxide in the gas chamber.

[0008] Preferably, the water content control device is an adjustable relative humidity control system, which controls the relative humidity in the experimental environment.

[0009] Preferably, the abscisic acid release device is a micro-control system for releasing abscisic acid, which can set concentration gradient for multi-point regulation.

[0010] Preferably, the LED lighting device is an LED light bar with dimming function, simulating different light intensities and cycles.

[0011] Preferably, the observation platform supports access to a microscope or imaging system, including an intermediate seat, a pressing plate and a base, the pressing plate is arranged at the top of the intermediate seat, and the base is arranged at the bottom of the intermediate seat.

[0012] The intermediate seat is internally provided with an intermediate groove extending through the top and bottom, and the intermediate groove is provided with transparent plates one on both sides of the top and bottom.

[0013] The pressing plate is provided with a top groove in the middle, the top groove corresponds in position to the intermediate groove, and the top groove is provided with a transparent plate two on the lower side, the pressing plate is provided with pin holes two on both sides of the top groove, and the pin holes two are provided with pin rods, and the bottom of the pin rods is located in the pin holes one.

[0014] The base is fixed to the intermediate seat by two bolts arranged on the left and right sides, and the base is provided with a bottom groove directly below the intermediate groove.

[0015] Preferably, the device for observing the movement of stomata is adjusted and monitored by a computer control system, and the changes of carbon dioxide concentration, moisture content, light intensity and plant hormone concentration are displayed in real time.

[0016] The technical effects and advantages of the present application are as follows:

[0017] The present application can accurately control the release of carbon dioxide concentration, moisture content and plant hormones, thereby simulating the changes of stomata movement under different environmental conditions, adjusting the light conditions through the LED lighting device, further controlling the opening and closing of the stomata, providing real-time experimental data for plant physiology research, plant breeding and other fields, and having the characteristics of multi-factor regulation, real-time monitoring, high-precision control and wide application. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 is a structure diagram of a device for observing the movement of stomata of live plant leaves provided by the embodiment of the present application;

[0019] Figure 2 is a structure diagram of an observation platform in a device for observing the movement of stomata of live plant leaves provided by the embodiment of the present application Figure 1 ;

[0020] Figure 3 is a structure diagram of an observation platform in a device for observing the movement of stomata of live plant leaves provided by the embodiment of the present application Figure 2 ;

[0021] Figure 4is a front view of an observation platform in a device for dynamic observation of stomata movement of live plant leaves provided by the embodiment of the present application;

[0022] Figure 5 is an exploded view of an observation platform in a device for dynamic observation of stomata movement of live plant leaves provided by the embodiment of the present application;

[0023] Figure 6 is an exploded view of an observation platform in a device for dynamic observation of stomata movement of live plant leaves provided by the embodiment of the present application;

[0024] Figure 7 is an exploded view of an observation platform in a device for dynamic observation of stomata movement of live plant leaves provided by the embodiment of the present application.

[0025] in the figure:

[0026] 1, microscope; 2, observation platform; 3, LED lighting device; 4, display device; 5, carbon dioxide concentration control device; 6, moisture content control device; 7, abscisic acid release device; 8, carbon dioxide removal device; 9, water removal device; 10, oxygen / nitrogen mixed gas; 21, intermediate seat; 22, pressing plate; 23, base; 211, intermediate groove; 212, transparent plate one; 213, pin hole one; 214, mixed gas inlet and outlet; 221, top groove; 222, transparent plate two; 223, pin hole two; 224, pin rod; 231, bottom groove; 232, bolt. DETAILED DESCRIPTION

[0027] The utility model will be explained in further detail in combination with the drawings and specific embodiments. The embodiments of the utility model are given for example and description, and are not exhaustive or limit the utility model to the disclosed forms. Many modifications and changes are obvious to those skilled in the art. The embodiments are selected and described to better illustrate the principles and practical application of the utility model, and to enable those skilled in the art to understand the utility model so as to design various embodiments with various modifications suitable for specific purposes. Embodiment 1

[0028] Please refer to Figure 1 In the embodiment, a device for dynamic observation of stomata movement of live plant leaves is provided, which comprises a microscope 1, an observation platform 2 and an LED lighting device 3. The observation platform 2 is placed on the microscope 1, and the plant leaves are clamped and fixed on the observation platform 2. The plant leaves can be observed through the microscope 1, and the LED lighting device 3 provides light source for the plant leaves.

[0029] In the process of observing the plant leaves, the surrounding environment is supplied with the external filtered air, the external air passes through the carbon dioxide removing device 8 and the water removing device 9 in turn, so that the filtered air forms the oxygen / nitrogen mixed gas 10, the mixed gas is delivered into the environment of the plant leaves after passing through the display device 4, the display device 4 can detect the specific concentration content and the like, can record the data, and can specifically display the data of carbon dioxide, water, air temperature, atmospheric pressure, relative humidity and the like, wherein the carbon dioxide removing device 8 is provided with sodium hydroxide and sodium carbonate as the removing agent, and the water removing device 9 is provided with a water absorbing agent to absorb the water in the air;

[0030] Moreover, in the process of observing the plant leaves, the inducing mechanism is also included, which is composed of the carbon dioxide concentration control device 5, the water content control device 6 and the abscisic acid releasing device 7, is passed through the display device 4 before being delivered to the observation platform 2, so as to record the concentration content, and is passed through the display device 4 again after the action of the plant leaves, and the change of the concentration content is displayed again for recording;

[0031] In the experiment, the researchers place the plant leaves in the observation platform 2, adjust the inducing mechanism according to the experimental requirements, and then adjust the carbon dioxide concentration, water content and hormone level, adjust the light intensity through the LED light device 3, the plant leaves will show different stomatal opening and closing dynamics under different environmental conditions, and the stomatal movement is recorded in real time through the high-resolution microscope or other imaging equipment, and further data analysis is carried out.

[0032] When directly observed through the microscope 1, the environmental parameters can be set as follows:

[0033] (1) Carbon dioxide control - low concentration induction open; high concentration induction off;

[0034] (2) Light: blue light LED stimulation induction open;

[0035] (3) Hormone: abscisic acid ABA induction off;

[0036] (4) Relative humidity: RH relative high humidity 85% open, low humidity off;

[0037] Carbon dioxide control induction, blue light LED stimulation induction, abscisic acid ABA induction are the main inductions, and the relative humidity is the secondary induction.

[0038] In the actual experiment, the carbon dioxide control - low concentration can be induced open alone, the blue light LED stimulation induction open, or the carbon dioxide control - low concentration induction open can be combined with the blue light LED stimulation induction open to be induced open together.

[0039] Carbon dioxide control - high concentration can be induced alone, abscisic acid ABA can be induced alone, or carbon dioxide control - high concentration induced closure combined with abscisic acid ABA induced closure together induced closure.

[0040] The carbon dioxide concentration control device 5 is used for adjusting the carbon dioxide concentration in the stomatal movement experiment, simulating different environmental climate conditions; the carbon dioxide concentration control device is an adjustable gas supply system, which can accurately control the concentration of carbon dioxide in the gas chamber.

[0041] The water content control device 6 is used for adjusting the relative humidity of the environment around the plant leaves, controlling the water content of the leaves, and simulating dry or humid environment; the water content control device is an adjustable relative humidity control system, which can control the relative humidity in the experimental environment.

[0042] The abscisic acid release device 7 is used for accurately controlling the release of abscisic acid ABA or other plant hormones, simulating the physiological changes of plants in response to water stress; the abscisic acid release device is a micro-control system capable of accurately releasing abscisic acid, which can be controlled by setting concentration gradient.

[0043] The LED light device 3 can simulate the photosynthesis and stomatal movement of plants under different light conditions by adjusting the light intensity and light period; the LED light device is a LED light bar with light adjusting function, which can simulate different light intensity and period, and adapt to the light demand of various plants.

[0044] The observation platform 2 is used for fixing the plant sample, so that the leaves are in the appropriate position for high-precision observation.

[0045] The whole device is adjusted and monitored by a computer control system, which can display the changes of carbon dioxide concentration, water content, light intensity and plant hormone concentration in real time.

[0046] The utility model can realize the following effects:

[0047] Multi-factor regulation: multiple environmental factors (such as carbon dioxide concentration, water content, hormone level and light intensity) can be regulated at the same time, providing more comprehensive experimental conditions for comprehensive research on stomatal movement.

[0048] Real-time monitoring: through the precise observation platform 2 and imaging equipment (microscope 1 or other imaging equipment), the opening and closing state of the stomata can be observed in real time, providing data support for quantitative research on the dynamic of stomatal movement.

[0049] High-precision control: the control precision of each parameter is high, which can simulate the response of plant stomata under various environmental changes and meet different research needs.

[0050] Wide application: suitable for plant physiology research, plant ecology research, agricultural production management and other fields.

[0051] The utility model discloses can accurate control carbon dioxide concentration, moisture content and plant hormone (such as abscisic acid) release to the change of stoma movement under different environmental conditions is simulated, the adjustment of illumination condition is realized through LED lighting device 3, further control the opening and closing of stoma, can provide real-time experimental data for the field of plant physiology research, plant breeding etc. Example 2

[0052] Please refer to Figures 2 to 7 In the device for dynamically observing stoma movement of live plant leaves provided in the embodiment, the observation platform 2 supports access to the microscope 1 or imaging system, thereby achieving observation and recording, and includes an intermediate seat 21, a pressing sheet 22 and a base 23, the pressing sheet 22 is arranged on the top of the intermediate seat 21, and the base 23 is mounted on the bottom of the intermediate seat 21,

[0053] In the intermediate seat 21, an intermediate groove 211 is arranged inside and penetrates through the intermediate seat 21 from top to bottom, transparent plates 212 are arranged on the upper and lower sides of the intermediate groove 211, so as to facilitate observation and illumination, meanwhile, pin holes 213 are arranged on the left and right sides of the upper end of the intermediate seat 21, and mixed gas inlets and outlets 214 are arranged on the left and right side walls of the intermediate seat 21, so that the mixed gas in the outside can be introduced into the hollow intermediate seat 21, thereby reacting with the plant leaves, one side of the mixed gas inlets and outlets 214 is an inlet, and the other side is an outlet.

[0054] A top groove 221 is arranged in the middle of the pressing sheet 22, the top groove 221 corresponds in position to the intermediate groove 211, and transparent plate 222 is arranged on the lower side of the top groove 221, the plant leaves can be placed on the transparent plate 212 on the upper side, then the plant leaves are fixed by being pressed through the transparent plate 222 on the pressing sheet 22, and the transparent material can be clearly observed, pin holes 223 are arranged on the left and right sides of the pressing sheet 22 located in the top groove 221, pin rods 224 are arranged in the pin holes 223, the bottom of the pin rods 224 is located in the pin holes 213, so that the pressing sheet 22 can be limited on the intermediate seat 21 through the pin rods 224, after the pin rods 224 are pulled out from the pin holes 213, the pressing sheet 22 can be moved and lifted, and then the plant leaves are placed, after the plant leaves are placed, the pin rods 224 are installed again, the plant leaves are fixed by being pressed through the transparent plate 222 on the pressing sheet, and the pressing sheet 22 is also limited and avoids shaking.

[0055] The base 23 is fixed with the intermediate seat 21 through the left and right two arranged bolts 232, and a bottom groove 231 is arranged below the intermediate groove 211 on the base 23, so that the bottom groove 231, the intermediate groove 211 and the top groove 211 form a through hole penetrating from top to bottom, and the through hole is partially sealed through the transparent material, so as not to affect the observation and light source passing through.

[0056] Obviously, the described embodiments are only a part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art and related fields without creative labor shall belong to the scope of protection of the present application. The structures, devices and operation methods not specifically described and explained in the present application, if not specially described and limited, are implemented according to the conventional means in the art.

Claims

1. A device for dynamically observing the stomatal movement of living plant leaves, characterized in that, Includes a microscope (1), an observation platform (2), and an LED lighting device (3). The observation platform (2) is placed on the microscope (1), and the plant leaves are clamped and fixed on the observation platform (2). The LED lighting device (3) provides a light source for the plant leaves. During the observation of plant leaves, filtered air is introduced into the surrounding environment. The air passes through a carbon dioxide removal device (8) and a water removal device (9) to obtain an oxygen / nitrogen mixture (10). The mixture is then transported to the plant leaf environment after passing through a display device (4). After passing through the plant leaves, the mixture passes through the display device (4) again. During the observation of plant leaves, an induction mechanism is also provided. The induction mechanism consists of a carbon dioxide concentration control device (5), a water content control device (6), and an abscisic acid release device (7). After being connected to the display device (4), it is transported to the observation platform (2).

2. The device for dynamic observation of stomatal movement in living plant leaves according to claim 1, characterized in that, The carbon dioxide removal device (8) contains sodium hydroxide and sodium carbonate, and the dehydration device (9) contains a dehydrating agent.

3. The device for dynamic observation of stomatal movement in living plant leaves according to claim 1, characterized in that, The carbon dioxide concentration control device (5) is an adjustable gas supply system that regulates the concentration of carbon dioxide in the gas chamber.

4. The device for dynamic observation of stomatal movement in living plant leaves according to claim 1, characterized in that, The moisture content control device (6) is an adjustable relative humidity control system that controls the relative humidity in the experimental environment.

5. The device for dynamic observation of stomatal movement in living plant leaves according to claim 1, characterized in that, The abscisic acid release device (7) is a micro-control system for releasing abscisic acid, which can be set with a concentration gradient for multi-point regulation.

6. The device for dynamic observation of stomatal movement in living plant leaves according to claim 1, characterized in that, The LED lighting device (3) is an LED light strip with dimming function, which simulates different light intensities and cycles.

7. The device for dynamic observation of stomatal movement in living plant leaves according to claim 1, characterized in that, The observation platform (2) supports access to a microscope (1) or an imaging system, including a middle seat (21), a pressing plate (22) and a base (23). The pressing plate (22) is set on the top of the middle seat (21), and the base (23) is installed at the bottom of it. The middle seat (21) has a through-hole (211) inside. The upper and lower sides of the middle seat (211) are provided with transparent plates (212). The upper end of the middle seat (21) is provided with pin holes (213) on the left and right sides of the middle seat (211). The left and right side walls of the middle seat (21) are provided with mixed gas inlet and outlet (214). The tablet (22) has a top groove (221) in the middle, which corresponds to the middle groove (211). A transparent plate (222) is provided on the lower side of the top groove (221). The tablet (22) has pin holes (223) on the left and right sides of the top groove (221). A pin (224) is provided in the pin hole (223), and the bottom of the pin (224) is located in the pin hole (213). The base (23) is fixed to the middle seat (21) by two bolts (232) on the left and right sides. The base (23) has a bottom groove (231) located directly below the middle groove (211).

8. A device for observing the dynamic movement of stomata in living plant leaves according to any one of claims 1-7, characterized in that, The device for dynamic observation of stomatal movement is regulated and monitored by a computer control system, which displays changes in carbon dioxide concentration, water content, light intensity, and plant hormone concentration in real time.

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