Lateral immunochromatography quantitative reagent

Abstract

The utility model relates to the technical field of in-vitro diagnosis and medical application, and discloses a lateral immunochromatography quantitative reagent which comprises a bottom plate, a sample pad is fixedly connected to the rightmost side of the upper portion of the bottom plate, a blood filtering pad is arranged on the left portion of the sample pad, a combining pad is arranged on the left portion of the blood filtering pad, and the combining pad is arranged on the right portion of the blood filtering pad. An NC membrane is arranged at the left part of the combination pad, a water absorption pad is arranged at the left part of the NC membrane, the NC membrane is coated with a detection antigen and is used for fixing a target substance in a sample, the sample pad is used for receiving and primarily treating the sample to be detected, and the sample pad and the blood filtering pad are fixed through an adhesive. According to the utility model, when whole blood and peripheral blood are detected, the content of folic acid in the body of a person to be detected can be obtained without centrifugation and red blood cell counting, so that folic acid detection can be realized in primary hospitals and village and town clinics.

Description

TECHNICAL FIELD

[0001] The utility model relates to in-vitro diagnosis and medical application technical field, especially relate to a lateral immunochromatography quantitative reagent. BACKGROUND

[0002] Folic acid mainly exists in red blood cells, and a part of folic acid also exists in serum plasma, and serum plasma folic acid detection provides an early indicator of the folic acid state in the body.

[0003] Folic acid mainly exists in the form of folic acid binding protein binding in the body, and folic acid needs to be dissociated from folic acid binding protein during detection, and red blood cells will be broken during the dissociation process, and the release of hematin and red blood cell folic acid from the broken red blood cells will affect the detection result due to the red background in the detection window, and the content of red blood cell folic acid is much higher than that of serum plasma folic acid, and if the red blood cells are broken, the actual detection result is mainly the content of red blood cell folic acid, and the content of red blood cell folic acid is often detected together with red blood cell count, and the amount of folic acid is obtained by multiplying the folic acid concentration and the red blood cell number, which has certain detection significance.

[0004] However, most primary hospitals cannot perform red blood cell counting. Therefore, a lateral immunochromatography quantitative reagent is provided to solve the problems in the above background art. UTILITY MODEL CONTENT

[0005] In order to make up for the above shortcomings, the utility model provides a lateral immunochromatography quantitative reagent, which aims to improve the problem that red blood cell counting cannot be performed in the prior art.

[0006] To achieve the above purpose, the utility model provides the following technical scheme: a lateral immunochromatography quantitative reagent, comprising a bottom plate, a sample pad is fixedly connected to the uppermost right side of the bottom plate, a sample pad is arranged on the left part of the sample pad, a blood filter pad is arranged on the left part of the sample pad, a combination pad is arranged on the left part of the blood filter pad, an NC membrane is arranged on the left part of the combination pad, and a water absorption pad is arranged on the left part of the NC membrane.

[0007] Further, the NC membrane is coated with a detection antigen for fixing the target substance in the sample.

[0008] Further, the sample pad is used for receiving and preliminarily processing the sample to be tested, and the sample pad and the blood filter pad are fixed through an adhesive.

[0009] Further, the blood filter pad is used for filtering solid particles in the sample and transmitting the liquid sample to the combination pad.

[0010] Further, the sample pad and the blood filter pad are fixed through an adhesive.

[0011] Further, the binding pad and the NC membrane are fixed by an adhesive.

[0012] The utility model has the advantages of the following:

[0013] 1、 the utility model discloses, user drops to be measured sample on sample pad, and sample pad absorbs sample liquid and begins to preliminarily process sample, and sample pad passes sample liquid to filter blood pad, and possibly carries out preliminary dilution or mixing to sample, and filter blood pad filters solid particles in sample, and the liquid sample after filtration flows into binding pad, and the compound on binding pad then moves to NC membrane, and on NC membrane, material antibody compound and coated detection antigen on membrane occur specific binding, thereby when detecting whole blood and peripheral blood, need not centrifugation, and also need not carry out red blood cell counting to obtain the folate content in the body of the person to be tested, make basic-level hospital and township clinic can realize folate detection. BRIEF DESCRIPTION OF DRAWINGS

[0014] Figure 1 A side view of the lateral flow immunochromatographic quantitative reagent is provided for the utility model;

[0015] Figure 2 A side view of the lateral flow immunochromatographic quantitative reagent is provided for the utility model;

[0016] LEGEND:

[0017] 1, bottom plate, 2, sample pad, 3, filter blood pad, 4, binding pad, 5, NC membrane, 6, water absorption pad. DETAILED DESCRIPTION

[0018] The technical solutions in the embodiments of the utility model will be apparently and completely described in combination with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.

[0019] Referring to Figure 1 and Figure 2 An embodiment provided by the utility model: a lateral flow immunochromatographic quantitative reagent, including bottom plate 1, the uppermost right side of bottom plate 1 is fixedly connected with sample pad 2, the left part of sample pad 2 is provided with sample pad 2, the left part of sample pad 2 is provided with filter blood pad 3, the left part of filter blood pad 3 is provided with binding pad 4, the left part of binding pad 4 is provided with NC membrane 5, and the left part of NC membrane 5 is provided with water absorption pad 6.

[0020] The bottom plate 1 is used for providing a place for supporting and connecting each part, the sample pad 2 is used for removing red blood cells in whole blood and peripheral blood, the blood filter pad 3 is connected with the sample pad 2 and the binding pad 4, further filters out the residual red blood cells in the sample, and meanwhile isolates the binding pad 4 from the sample pad 2, the binding pad 4 is used for connecting the blood filter pad 3 and the nitrocellulose membrane, contains a dissociation agent, makes folic acid dissociate from folic acid binding protein, changes from a bound state to a free state, and achieves the purpose of being detectable; meanwhile, the binding pad 4 contains fluorescent microsphere labeled antibodies, the NC membrane 5 is used for connecting the binding pad 4 and the water absorption pad 6, provides a reaction place, and a specific area is fixed with detection antigens (antibodies), and can fix folic acid in the sample, and the water absorption pad 6 is used for providing liquid chromatography power and storing excess liquid.

[0021] Referring to Figure 1 and Figure 2 , the NC membrane 5 is coated with detection antigens, and is used for fixing target substances in the sample, the sample pad 2 is used for receiving and preliminarily processing the sample to be detected, the sample pad 2 is fixed with the blood filter pad 3 through an adhesive, the blood filter pad 3 is used for filtering solid particles in the sample, and transmitting the liquid sample to the binding pad 4, the sample pad 2 and the blood filter pad 3 are fixed through an adhesive, and the binding pad 4 and the NC membrane 5 are fixed through an adhesive.

[0022] Working principle: when the device is used, the bottom plate 1 can provide a place for supporting and connecting each part, the sample pad 2 contains substances such as red blood cell antibodies which can fix red blood cells in the sample pad 2; the sample pad 2 is made of glass fiber, polyester film and other substances; the blood filter pad 3 is connected with the sample pad 2, further filters out the residual red blood cells in the sample, and meanwhile isolates the binding pad 4 from the sample pad 2; the binding pad 4 contains a dissociation agent and antibody labeled fluorescent microspheres; the NC membrane 5 contains coated antigens (antibodies); the water absorption pad 6 provides liquid chromatography power and stores excess liquid, so that when whole blood and peripheral blood are detected, the folic acid content in the body of the person to be detected can be obtained without centrifugation and red blood cell counting, and the folic acid detection can be realized in primary hospitals and township clinics.

[0023] Finally, it should be noted that: the above only for preferred embodiments of the present application, and not for limiting the present application, although the present application has been described in detail with reference to the foregoing embodiments, for those skilled in the art, it still can modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement for part of the technical features, any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application, should be included in the protection scope of the present application.

Claims

1. A lateral immunochromatographic quantitative test reagent comprising a base plate (1), characterized in that: The upper right side of the bottom plate (1) is fixedly connected with a sample pad (2), the left part of the sample pad (2) is provided with a sample pad (2), the left part of the sample pad (2) is provided with a blood filter pad (3), the left part of the blood filter pad (3) is provided with a combination pad (4), the left part of the combination pad (4) is provided with an NC membrane (5), and the left part of the NC membrane (5) is provided with a water absorption pad (6).

2. The lateral immunochromatographic quantitative reagent according to claim 1, characterized in that: The NC membrane (5) is coated with a detection antigen for fixing target substances in the sample.

3. The lateral immunochromatographic quantitative reagent according to claim 1, characterized in that: The sample pad (2) is used for receiving and preliminarily processing the sample to be tested, and the sample pad (2) is fixed with the blood filter pad (3) through an adhesive.

4. The lateral immunochromatographic quantitative reagent according to claim 1, characterized in that: The blood filter pad (3) is used for filtering solid particles in the sample and transmitting the liquid sample to the combination pad (4).

5. The lateral immunochromatographic quantitative reagent according to claim 1, characterized in that: The sample pad (2) and the blood filter pad (3) are fixed through an adhesive.

6. The lateral immunochromatographic quantitative reagent according to claim 1, characterized in that: The combination pad (4) and the NC membrane (5) are fixed through an adhesive.

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