Exhaust defoaming device for cell culture tank
By designing a defoaming box and defoaming mesh in a small benchtop reactor, the problem of filter clogging caused by foam generation was solved, achieving a simplified defoaming process and ease of operation.
Patent Information
- Application Number
- CN202423225303.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-25
- Publication Date
- 2025-12-16
- Estimated Expiration
- 2034-12-25
AI Technical Summary
Foam generation in small benchtop reactors leads to filter clogging, and existing defoaming methods require large equipment space and are difficult to operate.
Design a defoaming device for venting cell culture tanks, including a defoaming box connected in series with the vent pipe. The defoaming box is filled with defoaming mesh. The gas drives the bubbles to be cut and broken, and the culture medium flows back into the culture tank. The defoaming device has a simple structure, small size, and is easy to operate.
The defoaming process is simplified, the equipment is small in size, easy to operate, and adaptable to culture tanks with different gas flow rates, thus improving the applicability and flexibility of the defoaming device.
Smart Images
Figure CN223674663U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of biological cell culture, in particular to a cell culture tank exhaust defoaming device. BACKGROUND
[0002] In the process of cell culture, due to the influence of culture medium composition, stirring, aeration and other factors, a large amount of foam is often produced, which is easy to cause filter blockage.
[0003] The commonly used method is to add a defoaming agent or install a condenser in the exhaust pipeline to make the foam condense backflow, but a cooling water circulation system is needed to provide the condenser with condensing function, which is commonly used in large stainless steel bioreactors, but for small benchtop reactors, the equipment occupies too much space and is not easy to operate. The present application provides a small and simple device which can play a defoaming role. CONTENT OF THE UTILITY MODEL
[0004] In order to improve the problem that the equipment occupies too much space and is not easy to operate for small benchtop reactors, the present application provides a cell culture tank exhaust defoaming device.
[0005] The cell culture tank exhaust defoaming device provided by the present application adopts the following technical scheme:
[0006] A cell culture tank exhaust defoaming device, comprising a defoaming box connected in series on an exhaust pipe, the defoaming box is located between an exhaust filter and a culture tank, a defoaming cavity for gas flow is arranged in the defoaming box, and a defoaming wire mesh is filled in the defoaming cavity.
[0007] By adopting the above technical scheme, when the gas drives the bubbles into the defoaming cavity in the defoaming box along the exhaust pipe, the defoaming wire mesh cuts and breaks the bubbles, and the culture solution is backflowed to the culture tank along the exhaust pipe. Compared with the condenser defoaming method, the defoaming device has simple structure, small equipment volume, simplified defoaming process and easy operation.
[0008] In a specific implementable scheme, the defoaming box comprises an upper box body and a lower box body, the upper box body and the lower box body are connected through a connecting structure, the lower box body is connected with the exhaust pipe through a lower connecting pipe, and the upper box body is connected with the exhaust pipe through an upper connecting pipe.
[0009] In a specific implementable scheme, the connecting structure is ultrasonic welding.
[0010] In a specific implementable scheme, the connecting structure comprises a clamp and a sealing gasket, the sealing gasket is arranged between the upper box body and the lower box body, and the upper box body and the lower box body are clamped through the clamp.
[0011] By adopting the technical scheme, the upper box body and the lower box body are clamped by the clamp, the upper box body and the lower box body are convenient to disassemble, and the upper box body and the lower box body are sealed by the sealing gasket, and the sealing property of the defoaming device is improved.
[0012] In one specific implementation, the upper connecting pipe and the lower connecting pipe are both lotus-shaped joints with a gradually increasing diameter from a free end to a connecting end.
[0013] By adopting the technical scheme, the upper connecting pipe and the lower connecting pipe are both lotus-shaped joints, which can be connected with pipes of different diameters, and the flexibility of the connection between the upper connecting pipe, the lower connecting pipe and the pipes is improved.
[0014] In one specific implementation, the connecting structure comprises a connecting cylinder arranged on the upper box body and a connecting part arranged on the lower box body, the connecting part is capable of being inserted into the connecting cylinder, an outer thread is arranged on the inner side wall of the connecting cylinder, and an inner thread is arranged on the outer side of the connecting part and engaged with the outer thread.
[0015] By adopting the technical scheme, when the upper box body and the lower box body are connected, the connecting part is inserted into the connecting cylinder, and the connecting part is screwed, so that the inner thread is engaged with the outer thread, and the detachable connection between the upper box body and the lower box body is realized.
[0016] In one specific implementation, a first sealing annular surface is arranged on the connecting part, a sealing ring is arranged on the connecting part, a second sealing annular surface is arranged on the connecting cylinder, and the connecting part can tightly seal the sealing ring against the first sealing annular surface and the second sealing annular surface, so that the connecting cylinder and the connecting part are sealed.
[0017] By adopting the technical scheme, when the connecting part is screwed into the connecting cylinder, the sealing ring is tightly pressed against the second sealing annular surface, and the first sealing annular surface abuts against the sealing ring, so that the sealing ring is tightly sealed against the first sealing annular surface and the second sealing annular surface, and the sealing effect of the connecting part and the connecting cylinder is improved.
[0018] In one specific implementation, a first sealing groove for placing the sealing ring is arranged on the first sealing annular surface.
[0019] By adopting the technical scheme, the stability of the connection of the sealing ring on the connecting part is improved by arranging the first sealing groove.
[0020] In one specific implementation, a first arc-shaped surface matched with the sealing ring is arranged on the groove bottom wall of the first sealing groove.
[0021] By adopting the technical scheme, the first arc surface is matched with the sealing ring, the contact area of the sealing ring and the connecting portion is increased, and the sealing effect of the sealing ring is improved.
[0022] In one specific implementation, the second sealing groove is arranged on the second sealing ring surface.
[0023] The groove bottom wall of the second sealing groove is provided with a second arc surface matched with the sealing ring.
[0024] By adopting the technical scheme, the sealing ring is inserted into the second sealing groove, the stability of the sealing ring under extrusion is improved, the second arc surface is matched with the sealing ring, the contact area of the sealing ring and the connecting cylinder is increased, and the sealing effect of the sealing ring is improved.
[0025] In summary, the present application has at least one of the following beneficial technical effects:
[0026] 1. When the gas drives the bubbles into the defoaming cavity in the defoaming box along the exhaust pipe, the defoaming wire mesh cuts and breaks the bubbles, and the culture solution flows back to the culture tank along the exhaust pipe. Compared with the method of adding a condenser for defoaming, the defoaming device has a simple structure, a small equipment size, a simplified defoaming process, and is easy to operate.
[0027] 2. The upper box body and the lower box body are connected by a clamp, which facilitates replacement of the defoaming wire mesh in the defoaming box to adapt to different gas flow rates of the culture tank, and improves the applicability and flexibility of the defoaming device. BRIEF DESCRIPTION OF DRAWINGS
[0028] Figure 1 is a structural schematic view of a cell culture tank exhaust defoaming device according to Embodiment 1 of the present application.
[0029] Figure 2 is a structural schematic view of a cell culture tank exhaust defoaming device according to Embodiment 2 of the present application.
[0030] Figure 3 is a structural schematic view of a cell culture tank exhaust defoaming device according to Embodiment 3 of the present application. Figure 2 is a sectional view along line A-A in FIG.
[0031] Figure 4 is a structural schematic view of a cell culture tank exhaust defoaming device according to Embodiment 2 of the present application.
[0032] Figure 5 is a sectional view along line B-B in FIG. Figure 4
[0033] Figure 6 is a structural schematic view of a cell culture tank exhaust defoaming device according to Embodiment 3 of the present application.
[0034] Figure 7 is a sectional view along line C-C in FIG.Figure 6 Sectional view of the middle C-C line.
[0035] Figure 8 is Figure 7 Enlarged view of D in
[0036] Reference signs: 1, exhaust pipe; 2, defoaming box; 21, upper box body; 22, lower box body; 23, lower connecting pipe; 24, upper connecting pipe; 25, defoaming cavity; 3, culture tank; 4, connecting structure; 41, connecting cylinder; 411, matching section; 412, sealing section; 413, second sealing ring surface; 414, second sealing groove; 415, first arc surface; 42, connecting part; 421, connecting section; 422, plug-in section; 423, first sealing ring surface; 424, first sealing groove; 425, first arc surface; 43, sealing ring; 44, clamp; 45, sealing gasket; 5, defoaming wire mesh; 6, exhaust filter. DETAILED DESCRIPTION
[0037] The following will be described in detail in combination with the accompanying Figures 1-8 The present application is further described in detail.
[0038] The embodiment of the present application discloses a cell culture tank exhaust defoaming device.
[0039] Embodiment 1
[0040] Referring to Figure 1 , Figure 2 A cell culture tank exhaust defoaming device comprises a defoaming box 2 connected in series on an exhaust pipe 1, the defoaming box 2 is located between an exhaust filter 6 and a culture tank 3, the defoaming box 2 comprises an upper box body 21 and a lower box body 22, the upper box body 21 and the lower box body 22 are both transparent plastic materials, so that the bubble condition in the defoaming box 2 can be observed, the lower box body 22 is connected with the exhaust pipe 1 through a lower connecting pipe 23, and the upper box body 21 is connected with the exhaust pipe 1 through an upper connecting pipe 24.
[0041] Referring to Figure 2 , Figure 3 The upper box body 21 and the lower box body 22 are connected through a connecting structure 4, the connecting structure 4 in the embodiment is ultrasonic welding, and the welding mode can also adopt hot melt welding. The upper box body 21 and the lower box body 22 jointly enclose a defoaming cavity 25 for gas circulation, the defoaming cavity 25 is filled with a defoaming wire mesh 5, the defoaming wire mesh 5 in the embodiment is plastic material, and the defoaming wire mesh 5 is a sheet structure, so that the bubbles can be better cut.
[0042] The top of the upper connecting pipe 24 is a free end, one end of the upper box body 21 connected is a connecting end, the bottom of the lower connecting pipe 23 is a free end, and one end connected with the lower box body 22 is a connecting end. The upper connecting pipe 24 and the lower connecting pipe 23 are both lotus-shaped joints with a gradually increasing diameter from the free end to the connecting end. The upper connecting pipe 24 and the lower connecting pipe 23 are both set as lotus-shaped joints, which can be connected with pipes of different diameters, improving the flexibility of the connection between the upper connecting pipe 24, the lower connecting pipe 23 and the pipe.
[0043] The implementation principle of example 1 is as follows: when the gas drives the bubbles into the defoaming cavity 25 in the defoaming box 2 along the exhaust pipe 1, the defoaming wire mesh 5 cuts and breaks the bubbles, and the culture solution flows back to the culture tank 3 along the exhaust pipe 1. Compared with the method of adding a condenser for defoaming, the defoaming device has a simple structure, a small equipment volume, a simplified defoaming process and easy operation.
[0044] Example 2
[0045] With reference to Figure 4 , Figure 5 The difference between this embodiment and example 1 is that the connecting structure in this embodiment includes a clamp 44 and a sealing gasket 45. The clamp in this embodiment is a 304 stainless steel sanitary clamp joint. The connecting end of the upper box body 21 and the lower box body 22 is formed as a chuck structure. The clamp 44 is sleeved on the chuck structure and is fixed by screwing the bolt head to complete the clamping and fixing of the clamp 44. The sealing gasket 45 is installed between the upper box body 21 and the lower box body 22. After the clamp 44 is fixed, the upper box body 21 and the lower box body 22 are pulled to press the sealing gasket 45 together, so as to improve the sealing effect of the upper box body 21 and the lower box body 22.
[0046] Example 3
[0047] With reference to Figure 6 , Figure 7 The difference between this embodiment and example 1 is that the connecting structure 4 in this embodiment includes a connecting cylinder 41 arranged on the upper box body 21 and a connecting part 42 arranged on the lower box body 22. The materials of the connecting cylinder 41 and the connecting part 42 are the same as those of the upper box body 21 and the lower box body 22. The connecting cylinder 41 is fixedly arranged at the opening end of the upper box body 21 and communicates with the upper box body 21. The connecting part 42 is in a cylindrical shape. The connecting part 42 is fixedly arranged at the opening end of the lower box body 22 and is arranged in communication with the lower box body 22. The outer diameter of the connecting part 42 is equal to the inner diameter of the connecting cylinder 41, so that the connecting part 42 can be inserted into the connecting cylinder 41. The inner side wall of the connecting cylinder 41 is provided with external threads, and the outer side of the connecting part 42 is provided with internal threads engaged with the external threads.
[0048] When connecting the upper box 21 and the lower box 22, the connecting part 42 is inserted into the connecting cylinder 41 and screwed on, so that the internal thread and the external thread engage, thereby achieving a detachable connection between the upper box 21 and the lower box 22. This detachable connection facilitates the replacement of the defoaming mesh 5 inside the defoaming box 2, adapting to different gas flow rates in the culture tank 3 and improving the applicability and flexibility of the defoaming device.
[0049] Reference Figure 7 , Figure 8 The connecting part 42 includes a connecting section 421 and a plug-in section 422. The outer diameter of the plug-in section 422 is smaller than the outer diameter of the connecting section 421. An internal thread is provided on the connecting section 421. A first sealing ring surface 423 is formed on the end wall of the connecting section 421. The connecting cylinder 41 includes a mating section 411 and a sealing section 412. The inner diameter of the sealing section 412 is smaller than the inner diameter of the mating section 411, so that a second sealing ring surface 413 can be formed on the end wall of the sealing section 412. A sealing ring 43 is fitted on the plug-in section 422. A first sealing groove 424 is provided on the first sealing ring surface 423. The bottom wall of the first sealing groove 424 is provided with a first arc-shaped surface 425 that mates with the sealing ring 43. When the sealing ring 43 is fitted on the plug-in section 422, it is partially inserted into the first sealing groove 424. A second sealing groove 414 is provided on the second sealing ring surface 413 for placing the sealing ring 43. The bottom wall of the second sealing groove 414 is provided with a second arc-shaped surface 415 that mates with the sealing ring 43. By inserting the sealing ring 43 into the second sealing groove 414, the stability of the sealing ring 43 under compression can be improved. By fitting the second arc-shaped surface 415 with the sealing ring 43, the contact area between the sealing ring 43 and the connecting cylinder 41 is increased, thereby improving the sealing effect of the sealing ring 43.
[0050] When the connecting part 42 is screwed into the connecting cylinder 41, it presses the sealing ring 43 onto the second sealing ring surface 413. At the same time, the first sealing ring surface 423 abuts against the sealing ring 43, so that the sealing ring 43 abuts against both the first sealing ring surface 423 and the second sealing ring surface 413, thereby improving the sealing effect between the connecting part 42 and the connecting cylinder 41.
[0051] The implementation principle of Example 3 is as follows: When connecting the upper box 21 and the lower box 22, the connecting part 42 is inserted into the connecting cylinder 41, and the connecting part 42 is screwed on so that the internal thread and the external thread mesh, thereby realizing the detachable connection between the upper box 21 and the lower box 22. The detachable connection between the upper box 21 and the lower box 22 facilitates the replacement of the defoaming mesh 5 in the defoaming box 2 to adapt to the incubator 3 with different gas flow rates, thereby improving the applicability and flexibility of the defoaming device.
[0052] The above are all preferred embodiments of the present application, and do not limit the protection scope of the present application, so that: all equivalent changes made according to the structure, shape, principle of the present application should be covered in the protection scope of the present application.
Claims
1. A degassing and defoaming device for a cell culture tank, characterized in that: Includes a defoaming box (2) connected in series on the exhaust pipe (1), the defoaming box (2) being located between the exhaust filter (6) and the culture tank (3), the defoaming box (2) having a defoaming chamber (25) for gas flow, the defoaming chamber (25) being filled with defoaming mesh (5).
2. The cell culture tank exhaust and defoaming device according to claim 1, characterized in that: The defoaming box (2) includes an upper box body (21) and a lower box body (22). The upper box body (21) and the lower box body (22) are connected by a connecting structure (4). The lower box body (22) is connected to the exhaust pipe (1) through a lower connecting pipe (23). The upper box body (21) is connected to the exhaust pipe (1) through an upper connecting pipe (24).
3. The cell culture tank exhaust and defoaming device according to claim 2, characterized in that: The connection structure (4) is ultrasonically welded.
4. The cell culture tank exhaust and defoaming device according to claim 2, characterized in that: The connection structure (4) includes a clamp (44) and a sealing gasket (45). The sealing gasket (45) is disposed between the upper box body (21) and the lower box body (22). The upper box body (21) and the lower box body (22) are connected by the clamp (44).
5. The cell culture tank exhaust and defoaming device according to claim 2, characterized in that: Both the upper connecting pipe (24) and the lower connecting pipe (23) are pagoda-shaped connectors that gradually thicken from the free end to the connecting end.
6. The cell culture tank exhaust and defoaming device according to claim 2, characterized in that: The connecting structure (4) includes a connecting cylinder (41) disposed on the upper box body (21) and a connecting part (42) disposed on the lower box body (22). The connecting part (42) can be inserted into the connecting cylinder (41). The inner side wall of the connecting cylinder (41) is provided with an external thread, and the outer side of the connecting part (42) is provided with an internal thread that meshes with the external thread.
7. The cell culture tank exhaust and defoaming device according to claim 6, characterized in that: The connecting part (42) is provided with a first sealing ring surface (423), and a sealing ring (43) is sleeved on the connecting part (42). The connecting cylinder (41) is provided with a second sealing ring surface (413). The connecting part (42) can simultaneously abut and seal the sealing ring (43) against the first sealing ring surface (423) and the second sealing ring surface (413) to seal the connecting cylinder (41) and the connecting part (42).
8. The cell culture tank exhaust and defoaming device according to claim 7, characterized in that: A first sealing groove (424) is provided on the first sealing ring surface (423) for placing the sealing ring (43).
9. The cell culture tank exhaust and defoaming device according to claim 8, characterized in that: The bottom wall of the first sealing groove (424) is provided with a first arc-shaped surface (425) that cooperates with the sealing ring (43).
10. The cell culture tank exhaust and defoaming device according to claim 7, characterized in that: A second sealing groove (414) is provided on the second sealing ring surface (413) for placing the sealing ring (43); The bottom wall of the second sealing groove (414) is provided with a second arc-shaped surface (415) that cooperates with the sealing ring (43).