Sample loading bottle for sample purification
By designing a sample bottle with a funnel-shaped body and a stable base, the problems of sample residue and cumbersome operation were solved, the accuracy of sample extraction and the safety of the experiment were achieved, and the purification efficiency and stability were improved.
Patent Information
- Application Number
- CN202520085592.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-13
- Publication Date
- 2025-12-30
- Estimated Expiration
- 2035-01-13
AI Technical Summary
In the process of protein chromatography purification, the use of small beakers or conical centrifuge tubes can lead to problems such as excessive sample residue, cumbersome operation, and poor stability, which affect the purification effect and experimental safety.
Design a sample loading bottle for small-sample purification, including a bottle body, a cap, and a base. The bottle body is funnel-shaped, the cap has a sampling hole and a sample addition hole, the base has a support surface that is flush with the placement plane, is made of transparent material, and is equipped with graduation lines and dustproof components. The sampling tube is nearly perpendicular to the bottom of the bottle, the base is heavier than the bottle body, and the base is made of PC and PE to enhance stability and safety.
It improves the accuracy and integrity of sample extraction, reduces residues, ensures experimental efficiency and safety, provides a stable operating environment, and reduces the risk of spillage.
Smart Images
Figure CN223732797U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of experimental equipment technology, and in particular to a sample bottle for small-sample purification. Background Technology
[0002] In protein chromatography purification, when the purification process is still in the pilot stage or the sample volume is relatively small, researchers often choose gravity columns for purification for simplicity. In this process, the sample storage container is particularly important. Generally, samples are stored in small beakers or centrifuge tubes. However, when using small beakers as containers, the relatively smooth bottom design leads to a larger amount of sample residue, which causes some inconvenience to the experimental operation. Especially when connected to a constant flow pump, the tubing itself may be aging or made of soft materials, and the smooth bottom design of the beaker makes it easy for the tubing to tilt. This tilting can allow air to enter the tubing, thus affecting the purification effect and efficiency.
[0003] To address this issue, some researchers choose to use pointed-bottom centrifuge tubes to hold samples. The pointed-bottom design does indeed reduce sample residue to some extent, as its pointed bottom guides the liquid flow more effectively. However, the use of pointed-bottom centrifuge tubes is not without its problems. Due to their unique shape, they cannot be placed directly on the lab bench for stable operation; therefore, they must be placed inside a small beaker and secured in other ways. This makes the operation relatively cumbersome, and due to improper securing or operational errors, pointed-bottom centrifuge tubes are prone to tipping over. This not only wastes samples but can also lead to experimental accidents, such as sample spillage and contamination of the experimental environment. Utility Model Content
[0004] In view of this, the purpose of this utility model is to overcome the shortcomings of related technologies and to provide a sample loading bottle for small sample purification.
[0005] This utility model provides the following technical solution:
[0006] A sample loading bottle for small-sample purification includes a bottle body, a cap, and a base.
[0007] The bottle body is used to hold samples. The bottom of the bottle body is funnel-shaped and has a lowest point. The bottle cap covers the upper opening of the bottle body and has a sampling hole and a sample addition hole. A sampling tube extends from the bottle cap into the bottle body. One end of the sampling tube is connected to the sampling hole, and the other end of the sampling tube extends to the vicinity of the lowest point of the bottle bottom. The base is installed at the bottom of the bottle body. The end face of the base facing away from the bottle body is a support surface, and the support surface is flush with the placement plane.
[0008] As a further improvement to the above technical solution, both the bottle body and the base are made of transparent material.
[0009] As a further improvement to the above technical solution, scale lines are evenly distributed on the outer side wall of the bottle.
[0010] As a further improvement to the above technical solution, the angle between the opening interface of the sampling tube near the bottom of the bottle and the axis of the sampling tube is less than 90°.
[0011] As a further improvement to the above technical solution, the supporting surface is a frosted surface.
[0012] As a further improvement to the above technical solution, the end face area of the support surface is larger than the cross-sectional area of the bottle body.
[0013] As a further improvement to the above technical solution, the weight of the base is greater than the weight of the bottle body.
[0014] As a further improvement to the above technical solution, a dustproof component is provided inside the sample feeding hole.
[0015] As a further improvement to the above technical solution, the dustproof component includes dustproof sheets, and multiple dustproof sheets are evenly distributed relative to the axis of the sample dispensing hole. The multiple dustproof sheets can be spliced together into a circle when not subjected to external force to seal the sample dispensing hole.
[0016] As a further improvement to the above technical solution, the bottle cap is fitted onto the bottle body via a threaded connection.
[0017] As a further improvement to the above technical solution, the outer wall of the bottle cap is provided with anti-slip texture.
[0018] As a further improvement to the above technical solution, the bottle cap is made of PE (polyethylene); the bottle body and the base are made of PC (polycarbonate).
[0019] Compared with related technologies, the beneficial effects of this utility model are:
[0020] The sample loading bottle for small-sample purification provided by this utility model allows operators to simply add samples into the bottle through the sample loading port on the cap. When it's necessary to extract the sample, the operator connects the sampling port on the cap to an extraction device, allowing the sample to be drawn out sequentially from the bottom of the bottle via a sampling tube. During this process, the sample flows smoothly along the sampling tube, passing through the sampling ports sequentially, and is ultimately completely extracted from the bottle. The entire operation is convenient and smooth, saving experimental time and improving experimental efficiency.
[0021] Furthermore, by designing the bottle bottom in a funnel shape, the sample continuously converges towards the lowest point of the bottle as it decreases, making it easier and more thorough to extract the sample from the bottle. This design not only reduces sample residue inside the bottle but also ensures the accuracy and integrity of sample extraction, thereby further improving the reliability of experimental results.
[0022] To further enhance the practicality and safety of the sample vials, a base was incorporated to ensure stable placement on a flat surface. This base design makes the vials more stable during placement, effectively preventing the risk of tipping over. This not only protects the integrity of the experimental equipment but also ensures safety during the experimental process.
[0023] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, preferred embodiments are described below in detail with reference to the accompanying drawings. Attached Figure Description
[0024] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.
[0025] Figure 1 This diagram shows a schematic view of the sample bottle used for small-sample purification in one embodiment of the present invention.
[0026] Explanation of key component symbols:
[0027] 100-Bottle body; 110-Bottle bottom; 120-Lowest end; 130-Graduation line; 200-Bottle cap; 210-Sampling hole; 220-Sampling hole; 230-Sampling tube; 240-Anti-slip texture; 300-Base; 310-Supporting surface; 400-Dustproof component. Detailed Implementation
[0028] The embodiments of this utility model are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this utility model, and should not be construed as limiting this utility model.
[0029] In the description of this utility model, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model.
[0030] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.
[0031] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.
[0032] In this utility model, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.
[0033] like Figure 1 As shown, this embodiment provides a sample loading bottle for small-sample purification, including a bottle body 100, a bottle cap 200, and a base 300.
[0034] The bottle body 100 is used to hold samples. The bottom 110 of the bottle body 100 is funnel-shaped and has a lowest point 120. The bottle cap 200 covers the upper opening of the bottle body 100. The bottle cap 200 has a sampling hole 210 and a sample addition hole 220. A sampling tube 230 extends from the bottle cap 200 into the bottle body 100. One end of the sampling tube 230 is connected to the sampling hole 210, and the other end of the sampling tube 230 extends to the vicinity of the lowest point 120 of the bottle bottom 110. The base 300 is installed at the bottom of the bottle body 100. Specifically, the base 300 is fixedly connected to the bottle body 100 by adhesive or snap-fit. The end face of the base 300 facing away from the bottle body 100 is a support surface 310, which is flush with the placement plane.
[0035] The sample loading bottle for small-sample purification provided in this embodiment allows the operator to simply add the sample into the bottle body 100 through the sample loading port 220 on the cap 200 during actual use. When it is necessary to extract the sample from the bottle body 100, the operator simply connects the sampling port 210 on the cap 200 to an extraction device, and the sample from the bottle body 100 is then extracted sequentially from the bottom 110 through the sampling tube 230. During this process, the sample flows smoothly along the sampling tube 230, passes through the sampling port 210 sequentially, and is finally completely extracted from the bottle body 100. The entire operation is convenient and smooth, saving experimental time and improving experimental efficiency.
[0036] Furthermore, by designing the bottle bottom 110 in a funnel shape, the sample continuously converges towards the lowest point 120 of the bottle bottom 110 during the reduction process. This allows the sampling tube 230 to more easily and thoroughly extract the sample from the bottle body 100. This design not only reduces sample residue in the bottle body 100 but also ensures the accuracy and integrity of sample extraction, thereby further improving the reliability of experimental results.
[0037] To further enhance the practicality and safety of the sample bottles, a base 300 is incorporated to ensure stable placement on a flat surface. This base 300 design makes the sample bottles more stable during placement, effectively preventing the risk of tipping over. This not only protects the integrity of the experimental equipment but also ensures safety during the experiment.
[0038] In some specific embodiments, both the bottle 100 and the base 300 are made of transparent material. This design allows the operator to clearly observe the sample state inside the bottle 100, including its color, transparency, sedimentation, and changes during the reaction process. The use of transparent material enables the operator to monitor the sample's state in real time, allowing for timely adjustments or appropriate measures to ensure the accuracy and reliability of the experiment.
[0039] Furthermore, the choice of transparent materials makes the entire experimental process more intuitive and visual, which is crucial for researchers. It not only improves the efficiency of experimental operations and reduces the possibility of misjudgment and errors, but also provides more accurate and detailed information for recording and analyzing experimental results.
[0040] In some specific embodiments, graduation lines 130 are evenly distributed on the outer wall of the bottle 100. The presence of these graduation lines 130 provides operators with an intuitive and accurate reference standard, enabling them to easily read and record the sample volume inside the bottle 100. By directly observing the graduation lines 130, operators can quickly obtain the specific value of the sample volume without the need for other measuring tools, thereby greatly improving the efficiency and accuracy of experimental operations.
[0041] Furthermore, these evenly distributed graduations 130 allow operators to more precisely control the amount of sample when adding or removing it, avoiding sample waste or experimental errors caused by improper operation. This is especially important for experiments requiring strict control of sample volume, ensuring the reliability and repeatability of experimental results.
[0042] In some specific embodiments, the angle between the opening interface of the sampling tube 230 near the bottom of the bottle 110 and the axis of the sampling tube 230 is less than 90°. This design expands the suction surface of the sampling tube 230, so that when the sample is drawn from the bottom of the bottle 110, the sampling tube 230 can make more full contact with the sample, thereby improving the efficiency and accuracy of sampling.
[0043] Meanwhile, this design also reduces the probability of the sampling tube 230 becoming blocked due to deformation and its opening coming into contact with the bottle bottom 110. In actual operation, due to various factors, the sampling tube 230 may sometimes undergo slight deformation. If the opening interface of the sampling tube 230 is perpendicular to the axis, the deformed sampling tube 230 can easily come into contact with the bottle bottom 110, causing the opening to be blocked and preventing successful sample extraction. By designing the opening interface to form an angle of less than 90° with the axis, even if the sampling tube 230 deforms, its opening is more likely to maintain a certain gap, thus avoiding blockage and ensuring the reliable operation of this embodiment.
[0044] In some specific embodiments, the support surface 310 is a frosted surface; this design cleverly utilizes the unique roughness of the frosted surface to increase the friction between the base 300 and the placement surface.
[0045] In practical use, when the base 300 is placed on an experimental table or other flat surface, the frosted surface can form a tight contact with the surface, and its rough surface structure effectively prevents relative sliding between the base 300 and the surface. This characteristic is crucial for ensuring the stable placement of experimental equipment, especially when conducting experiments requiring high stability and precision, as even the slightest slippage can have unpredictable effects on the experimental results.
[0046] Therefore, by designing the support surface 310 as a frosted surface, not only is the friction between the base 300 and the placement plane increased, but the stability of the placement during the experiment is also ensured, providing researchers with a more reliable and safe experimental environment.
[0047] In some specific embodiments, the end face area of the support surface 310 is larger than the cross-sectional area of the bottle body 100.
[0048] In some specific embodiments, the weight of the base 300 is greater than the weight of the bottle body 100. This design utilizes the principle of gravity in physics to reduce the probability of tipping over during use.
[0049] To achieve a design where the base 300 weighs more than the bottle body 100, several methods were employed. One method involved placing filler material between the base 300 and the bottle bottom 110. This filler material could be a high-density, high-strength material, such as metal particles, ceramic fragments, or specially made plastic blocks, which was evenly distributed throughout the gap between the base 300 and the bottle bottom 110, effectively increasing the weight of the base 300. Another method was to directly use high-density materials to manufacture the base 300. These materials possess excellent mechanical properties and weight characteristics, ensuring that the base 300 maintains structural strength while possessing sufficient weight to support the entire device.
[0050] By making the weight of the base 300 greater than that of the bottle 100, the overall center of gravity of this embodiment is lowered, which helps reduce the risk of the device tipping over when subjected to external forces. In practical use, whether conducting experiments or storing and transporting the equipment, this design provides researchers with a more stable and reliable experimental environment, ensuring the accuracy and safety of experimental results.
[0051] In some specific embodiments, a dustproof component 400 is provided inside the sample dispensing hole 220; this design enables the sample dispensing hole 220 to be effectively sealed when it is not necessary to input samples into the bottle 100, thereby preventing foreign objects, dust or impurities from entering the bottle 100 and causing contamination of the sample or interfering with the experimental results.
[0052] Specifically, the dustproof assembly 400 includes multiple dustproof sheets. These dustproof sheets are evenly distributed relative to the axis of the sample application hole 220, and their number can be flexibly adjusted according to actual needs. When not subjected to external force, the multiple dustproof sheets can be joined together to form a complete circle, tightly fitting the opening of the sample application hole 220, achieving an effective sealing effect. This design is not only simple in structure and easy to implement, but also convenient to use, allowing for easy sealing and opening of the sample application hole 220 without additional operations or tools.
[0053] It is worth mentioning that although the multiple dustproof sheets fit together tightly when not subjected to external force, tiny gaps still exist between them. These gaps play a crucial role when samples need to be drawn from the bottle 100. They allow the air pressure inside and outside the bottle 100 to balance, thus avoiding difficulties or obstructions in sample extraction caused by pressure differences. This design ensures both dustproof effectiveness and smooth sample extraction, achieving a dual improvement in practicality and safety.
[0054] In some specific embodiments, the bottle cap 200 is threaded onto the bottle body 100; by rotating the bottle cap 200, it can be smoothly fitted onto the bottle body 100, achieving a tight seal. This design not only enhances the sealing performance of the bottle cap 200, preventing leakage of internal samples or intrusion of external contaminants, but also greatly facilitates the operator's quick opening and closing of the opening on the bottle body 100, improving work efficiency.
[0055] Furthermore, to optimize the user experience and prevent slippage when twisting the bottle cap 200, anti-slip textures 240 are added to the outer wall of the bottle cap 200. These anti-slip textures 240 can specifically be vertical stripes parallel to the axis of the bottle cap 200, evenly distributed on the side wall of the bottle cap 200, thus forming an effective friction surface. This allows the operator to obtain a more stable feel when rotating the bottle cap 200. Even in wet or oily conditions, the anti-slip textures 240 effectively increase the friction between the hand and the bottle cap 200, ensuring that the operator can easily and accurately complete the tightening or unscrewing of the bottle cap 200, further improving the convenience and safety of operation.
[0056] In some specific embodiments, the bottle cap 200 is made of PE (polyethylene), a material known for its excellent chemical stability, good sealing performance, and low cost. It effectively prevents sample leakage during storage and transportation and does not chemically react with most samples, thus ensuring sample integrity and accuracy. Furthermore, PE material possesses good flexibility and processing properties, allowing the bottle cap 200 to easily fit with the bottle body 100 for a tight seal. The bottle body 100 and the base 300 are made of PC (polycarbonate). PC material is renowned for its high strength, high transparency, excellent heat resistance, and impact resistance. It can withstand high pressure and temperature while maintaining good transparency, allowing operators to clearly observe the sample inside the bottle body 100. In addition, PC material has good processing properties and plasticity, enabling the bottle body 100 and base 300 to be designed in various shapes and sizes to meet the needs of different experimental scenarios.
[0057] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.
[0058] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.
Claims
1. A sample injection vial for small sample purification, characterized by, The utility model relates to a bottle body (100) for containing sample, the bottle bottom (110) of bottle body (100) is funnel-shaped, and the bottle bottom (110) has the lowest end (120). The bottle cap (200) is arranged on the upper opening of the bottle body (100), and the sampling hole (210) and the sample adding hole (220) are arranged on the bottle cap (200). The sampling tube (230) is arranged in the bottle cap (200) and extends into the bottle body (100). One end of the sampling tube (230) is communicated with the sampling hole (210), and the other end of the sampling tube (230) extends to the vicinity of the lowest end (120) of the bottle bottom (110). The base (300) is arranged on the bottom of the bottle body (100), and the end surface of the base (300) away from the bottle body (100) is the supporting surface (310). The supporting surface (310) is flush with the placement plane. The bottle body (100) and the base (300) are made of transparent material.
2. The loading vial for small scale purification according to claim 1, wherein The outer side wall of the bottle body (100) is uniformly provided with scale lines (130).
3. The loading vial for small scale purification according to claim 2, wherein The supporting surface (310) is frosted.
4. The loading vessel for small scale purification according to claim 1, wherein The area of the end surface of the supporting surface (310) is greater than the cross-sectional area of the bottle body (100).
5. The loading vessel for small scale purification according to claim 1, wherein The weight of the base (300) is greater than the weight of the bottle body (100).
6. The loading vessel for small scale purification according to claim 1, wherein The dustproof assembly (400) is arranged in the sample adding hole (220).
7. The loading vessel for small scale purification according to claim 1, wherein The dustproof assembly (400) includes dustproof sheets, and the dustproof sheets are uniformly distributed with respect to the axis of the sample adding hole (220). The dustproof sheets can be jointly spliced into a circle under the condition of no external force, and the sample adding hole (220) is blocked.
8. The loading cartridge of claim 7, wherein, The bottle cap (200) is sleeved on the bottle body (100) through thread cooperation.
9. The loading vessel for small scale purification according to any one of claims 1 to 8, wherein The outer side wall of the bottle cap (200) is provided with anti-slip lines (240).
10. The loading cartridge of claim 9, wherein,