High-efficiency separator for human sperm and spot mixed spots

Abstract

The utility model discloses an efficient separator for human seminal spot mixed spots, which relates to the technical field of separators and comprises a centrifugal tube and a detachable sleeve matched with the centrifugal tube, the caliber of the detachable sleeve is consistent with that of the centrifugal tube, and a sleeve cavity for containing biological material evidences is arranged in the detachable sleeve. A plastic filter plate is arranged in the middle of the sleeve cavity, a filter membrane filter layer is arranged at the bottom of the sleeve cavity, and a bottom drainage hole is formed in the bottom of the detachable sleeve. According to the utility model, a two-stage filtering mode of carrier removal filtering and sperm enrichment filtering is constructed in one sleeve, so that a biological material evidence carrier can be efficiently removed, water-soluble substances of human epithelial cells can be quickly filtered, sperm cells can be efficiently separated from related biological material evidences only by using a small amount of biological material evidences, and the operation is simple and convenient. The test speed and efficiency are greatly improved, difficult biological material evidence treatment is simplified, the experiment repeatability is good, the applicability is high, biological material evidence samples are saved, and the cost of related experiments is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of separators, and specifically relates to a high-efficiency separator for human semen stains and mixed stains. Background Technique

[0002] In the practice of forensic science work, human semen stains and mixed stains in sexual crimes are common and difficult-to-process biological evidence. Due to the special composition of such evidence itself and the key nature of the evidence, it has the characteristics of difficult testing and high value. Forensic science testers need to separate sperm cells in the body fluid mixed stains and then test the DNA template of the suspect. In practical work, the classic method is the two-step separation method, that is, after the biological evidence is experimentally digested and processed, the human epithelial cell components in the supernatant of the centrifuge tube and the sperm components in the bottom sediment are separated. However, the classic two-step separation method takes a long time, causes great loss of evidence, is not easy to guarantee the experimental success rate, and the results of parallel tests are not balanced; some current related optimization technologies are cumbersome to operate, have a long experimental process and high costs, and have certain limitations in practical applicability. Therefore, the utility model hereby proposes a high-efficiency separator for human semen stains and mixed stains. Content of the Utility Model

[0003] Technical Problems to be Solved

[0004] The purpose of the utility model is to make up for the deficiencies of the existing technology and provide a high-efficiency separator for human semen stains and mixed stains.

[0005] Technical Solution

[0006] To achieve the above purpose, the utility model provides the following technical solution: A high-efficiency separator for human semen stains and mixed stains includes a centrifuge tube and a detachable sleeve that matches the centrifuge tube. The caliber of the detachable sleeve is the same as that of the centrifuge tube. A sleeve lumen for containing biological evidence is provided in the detachable sleeve. A plastic filter plate is arranged in the middle of the sleeve lumen, and a filter membrane filtration layer is arranged at the bottom of the sleeve lumen. A bottom drainage hole is arranged at the bottom of the detachable sleeve. A two-stage filtration mode of removing the carrier filtration and enriching sperm filtration is constructed in one sleeve, which can efficiently remove the biological evidence carrier, quickly filter the water-soluble substances of human epithelial cells, and only a small amount of biological evidence can efficiently separate sperm cells from the relevant biological evidence, greatly improving the testing speed and efficiency, simplifying the processing of difficult biological evidence, having good experimental repeatability and strong applicability, saving biological evidence samples, and reducing the costs of related experiments.

[0007] Preferably, it further includes a tube lid that is common to the centrifuge tube and the sleeve, and the tube lid is adapted to the caliber of the detachable sleeve.

[0008] Preferably, the tube lid is connected to the centrifuge tube.

[0009] Preferably, the tube cap is connected to the sleeve.

[0010] Preferably, the plastic filter plate has filter holes.

[0011] Preferably, the diameter of the centrifuge tube is adapted to the lower edge of the detachable sleeve.

[0012] Beneficial effects:

[0013] Compared with existing technologies, this high-efficiency separator for mixed human semen stains has the following advantages:

[0014] This invention relates to a high-efficiency separator for mixed human sperm stains. It constructs a two-stage filtration mode within a single sleeve, consisting of carrier removal filtration and sperm enrichment filtration. This mode can efficiently remove biological evidence carriers and rapidly filter water-soluble substances from human epithelial cells. Only a small amount of biological evidence is required to efficiently separate sperm cells from relevant biological evidence, greatly improving the speed and efficiency of testing. It simplifies the processing of difficult biological evidence, has good experimental repeatability and strong applicability, saves biological evidence samples, and reduces the cost of related experiments. Attached Figure Description

[0015] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0016] Figure 1 This is a schematic diagram of the structure of this utility model;

[0017] Figure 2 This is a cross-sectional view of the detachable sleeve of this utility model.

[0018] Figure 3 This is a schematic diagram of the detachable sleeve of this utility model;

[0019] Figure 4 This is a schematic diagram of the centrifuge tube of this utility model;

[0020] Figure 5 This is a schematic diagram of one embodiment of the present invention.

[0021] In the picture:

[0022] 1. Centrifuge tube; 2. Removable sleeve; 3. Tube cap; 4. Sleeve cavity; 5. Plastic filter plate; 6. Filter membrane layer; 201. Lower edge; 202. Bottom drainage hole; 501. Filter hole. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0024] Please see Figures 1-5 As shown, this utility model provides a technical solution: a high-efficiency separator for mixed human semen stains, comprising an external 1.5 ml centrifuge tube 1 (4.0 cm in length, 1.4 cm in outer diameter, and 1.0 cm in inner diameter), and a detachable sleeve 2 (2.2 cm in length, 1.4 cm in outer diameter, 1.0 cm in inner diameter, 0.3 cm in height, and a liquid volume greater than 500 μL) matching the centrifuge tube 1. The diameter of the detachable sleeve 2 is the same as that of the centrifuge tube 1. The diameter of the centrifuge tube 1 is adapted to the lower edge 201 of the detachable sleeve 2. The detachable sleeve 2 is provided with a sleeve cavity 4 for holding biological evidence. A plastic filter plate 5 is provided in the middle of the sleeve cavity 4. The plastic filter plate 5 has filter holes 501. A filter membrane 6 with a certain degree of permeability is provided at the bottom of the sleeve cavity 4. The biological characteristics of human epithelial cells and sperm cells are used to separate different donors in the same mixed plaque. A bottom drainage hole 202 is provided at the bottom of the detachable sleeve 2.

[0025] Please refer to the following carefully. Figure 1 and Figure 5 It also includes a tube cap 3 that is universal for centrifuge tube 1 and sleeve 2, and the tube cap 3 is adapted to the diameter of the detachable sleeve 2.

[0026] In one embodiment, the tube cap 3 is connected to the centrifuge tube 1.

[0027] In one embodiment, the tube cap 3 is connected to the sleeve 2.

[0028] Working principle: (1) Combine the detachable sleeve 2 with the centrifuge tube 1, and add the target substance into the sleeve lumen of the detachable sleeve 2. (2) Place the device in a centrifuge for low-speed centrifugation. The DNA in human epithelial cells dissolves in the lysis buffer, while the sperm cells are preserved intact. Through centrifugation, the mixed plaque carrier is retained on the plastic filter plate 5, which can maximize the transfer of the target substance and facilitate the removal of the carrier. At the same time, the specific filter membrane 6 can flow into the bottom of the centrifuge tube through the digestion solution of the mixed plaque and the drainage hole 202 at the bottom of the sleeve. The filter membrane can enrich the sperm cells, thereby achieving rapid separation of the mixed plaque.

[0029] This patent provides a high-efficiency separator for mixed human sperm stains. By constructing a two-stage filtration system in one device, it can efficiently remove biological evidence carriers and quickly filter water-soluble substances from human epithelial cells. Only a small amount of biological evidence is needed to efficiently separate sperm cells from relevant biological evidence. Combined with the accompanying experimental methods, it achieves fast speed, high success rate, simple operation, and good stability in related experiments, making it suitable for general laboratories.

[0030] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0031] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A high-efficiency separator for mixed human semen stains, characterized in that: The application relates to a centrifugal tube (1), a detachable sleeve (2) matched with the centrifugal tube (1), wherein the diameter of the detachable sleeve (2) is consistent with that of the centrifugal tube (1), a sleeve cavity (4) for containing biological evidence is arranged in the detachable sleeve (2), a plastic filter plate (5) is arranged in the middle of the sleeve cavity (4), a filter membrane layer (6) is arranged at the bottom of the sleeve cavity (4), and a bottom drainage hole (202) is arranged at the bottom of the detachable sleeve (2).

2. A high efficiency separator of human sperm and mixed cells according to claim 1, characterized in that: The application also relates to a tube cover (3) matched with the centrifugal tube (1) and the detachable sleeve (2), wherein the diameter of the tube cover (3) is consistent with that of the detachable sleeve (2).

3. A high efficiency separator of human sperm and mixed cells according to claim 2, characterized in that: The tube cover (3) is connected with the centrifugal tube (1).

4. A high efficiency separator of human sperm and mixed cells according to claim 2, characterized in that: The tube cover (3) is connected with the detachable sleeve (2).

5. A high efficiency separator of human sperm and mixed cells according to claim 1, characterized in that: The plastic filter plate (5) is provided with filter holes (501).

6. A high efficiency separator of human sperm and mixed cells according to claim 1, characterized in that: The diameter of the centrifugal tube (1) is consistent with that of the lower edge (201) of the detachable sleeve (2).

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