Water sample biological counting device

Abstract

The utility model provides a biological counting device for a water sample. The biological counting device comprises a bottom plate and a cover glass, the top surface of the bottom plate is provided with at least one accommodating groove for accommodating a water sample, the periphery of the accommodating groove is annularly provided with a sealing groove, the sealing groove is filled with the water sample, the periphery of the sealing groove is provided with an overflow groove, and the overflow groove is used for collecting the water sample overflowing from the accommodating groove; the cover glass is connected to the top face of the bottom plate in a covering mode and covers the containing groove and the sealing groove. During volatilization, the water sample in the overflow groove is volatilized firstly, then the water sample in the sealing groove is volatilized, and finally the water sample in the containing groove is volatilized, so that the time of starting to volatilize the water sample in the containing groove can be delayed through sequential volatilization from outside to inside, it is ensured that the water sample in the containing groove cannot volatilize before counting is completed, and the accuracy of the counting result is improved; and compared with an oil seal mode, the cover glass is easy to clean, can be repeatedly used until the cover glass cannot be used, is high in repeated utilization rate, and can greatly reduce the cost.

Description

Technical Field

[0001] This utility model relates to the field of water analysis technology, and in particular to a water sample biological counting device. Background Technology

[0002] The organisms in the water samples include microscopic plants and animals, primarily algae and bacteria. Due to eutrophication, algae proliferate in large quantities in some lakes and reservoirs, including cyanobacteria blooms which can synthesize and release harmful metabolites such as algal toxins, dimethylisoborneol, and geosmin during their growth. These harmful substances not only affect the sensory properties of the water but can also have significant toxic effects on fish, shrimp, and even humans and livestock, seriously threatening water supply security. Bacteria also affect water quality. Monitoring the abundance of algae and bacteria plays a crucial role in ecological and environmental monitoring.

[0003] A typical water sample biological counting device consists of a counting frame, a glass slide, and a coverslip. The counting frame is fixed on the glass slide. During use, the water sample is dropped into the counting frame, and then the coverslip is placed flat on top of the counting frame. Pressing down on the water sample removes air bubbles from the counting frame. Finally, the counting device is placed under an optical microscope for observation and counting. Because manual identification and counting can be time-consuming, the water sample can easily evaporate between the counting frame and the coverslip, causing the sample edge to shrink towards the center within the counting frame. This can lead to the displacement of some organisms, resulting in double counting, undercounting, and omissions, directly affecting the accuracy of the final results.

[0004] To address the aforementioned evaporation issues, some existing technologies propose sealing by injecting oil. However, oil stains make cleaning the cover plate inconvenient, and the cover plate needs to be replaced after one or a few uses, resulting in a low reuse rate and significantly increasing costs. Utility Model Content

[0005] In view of this, the present invention proposes a water sample biological counting device to solve the technical problems mentioned in the background art, which are that sealing by injecting oil makes it inconvenient to clean the cover plate, the reusability of the cover glass is low, and the cost is greatly increased.

[0006] The technical solution of this utility model is implemented as follows:

[0007] This utility model provides a biological counting device for water samples, including a base plate and a cover glass, wherein:

[0008] The top surface of the base plate is provided with at least one receiving tank for holding water samples. The receiving tank is surrounded by a sealing groove, which is filled with water samples. An overflow groove is provided around the sealing groove to collect water samples that overflow from the receiving tank.

[0009] The cover glass is attached to the top surface of the base plate and covers the receiving groove and the sealing groove.

[0010] Based on the above technical solutions, preferably, the base plate is embedded with a magnet, the cover glass includes a cover frame and glass, the cover frame is made of magnetic material, the glass is sealed to the cover frame, and the visible area of ​​the glass is larger than the area where the receiving groove is located.

[0011] Based on the above technical solutions, preferably, the base plate includes an outer frame and an observation platform, the observation platform is located inside the outer frame, the outer frame has a groove cut on the outside of the observation platform to form the overflow groove, and the receiving groove and the sealing groove are both located on the top surface of the observation platform;

[0012] The cover frame has a positioning groove on the side facing the receiving groove, and the observation platform is nested in the positioning groove.

[0013] Based on the above technical solutions, preferably, the top surface of the base plate is provided with a recessed portion along the periphery of the overflow groove, and the two sides of the cover frame are provided with protrusions, the protrusions being placed in the recessed portion and protruding from the side of the base plate.

[0014] Based on the above technical solutions, preferably, the side of the base plate is provided with a side recess corresponding to the position of the recessed portion, and the protrusion protrudes from the inner sidewall of the side recess.

[0015] Based on the above technical solutions, preferably, there are two receiving grooves, two sealing grooves and two overflow grooves, and two cover glass sheets, with each cover glass sheet corresponding to one receiving groove.

[0016] Based on the above technical solutions, preferably, the top surface of the base plate is provided with focusing holes at both ends along the length direction.

[0017] Based on the above technical solutions, preferably, it also includes an installation fixture, which is provided with multiple clamping stations for clamping the base plate.

[0018] Based on the above technical solutions, preferably, the installation fixture includes a fixture frame and a clamp. The fixture frame is provided with a plurality of fixture slots. One side of each fixture slot is provided with a slot, and the clamp is installed on the other side. The clamp is slidably installed on the fixture frame, and a return spring is provided between the clamp and the inner wall of the fixture frame.

[0019] Based on the above technical solutions, preferably, the cross-section of the receiving groove is rectangular.

[0020] The water sample biological counting device of this invention has the following advantages over the prior art:

[0021] (1) A water sample is contained in a container. The container is surrounded by a sealing groove. The sealing groove is filled with water sample. An overflow groove is provided around the sealing groove. The overflow groove collects the water sample that overflows from the container. During evaporation, the water sample in the overflow groove evaporates first, then the water sample in the sealing groove evaporates, and finally the water sample in the container. By evaporating sequentially from the outside to the inside, the time when the water sample in the container begins to evaporate can be delayed, ensuring that the water sample in the container will not evaporate before the counting is completed, thus improving the accuracy of the counting results. Moreover, compared with the oil sealing method, the cover glass is easy to clean and can be reused until it is no longer usable. The high reuse rate can significantly reduce costs.

[0022] (2) The base plate is embedded with a magnet. The cover glass includes a cover frame and glass. The cover frame is made of magnetic material. When the cover glass is closed on the base plate, the magnet magnetically connects the cover frame, so that the cover frame and the base plate are tightly connected and the containment tank is sealed. The assembly and disassembly are relatively convenient and quick, which can improve the counting efficiency, shorten the counting time, and ensure that the water sample in the containment tank will not evaporate before the counting is completed.

[0023] (3) A positioning groove is provided on the side of the cover frame facing the receiving groove. The observation platform is nested in the positioning groove. The positioning groove is aligned with the observation platform and put on. With the magnetic attraction of the magnet, a quick connection can be completed. The positioning is convenient and quick, and the entire receiving groove can be seen from the position of the glass, which can improve the installation efficiency.

[0024] (4) A recessed portion is provided on the top surface of the bottom plate along the periphery of the overflow groove, and a protruding portion is provided on both sides of the cover frame. The protruding portion is placed in the recessed portion and protrudes from the side of the bottom plate, which makes it easier to pick up and put down the cover frame through the protruding portion, making the operation more convenient, improving the assembly and disassembly efficiency, and thus improving the counting efficiency.

[0025] (5) A side recess is provided on the side of the base plate corresponding to the position of the recess. The protrusion protrudes from the inner wall of the side recess. The protrusion does not need to extend beyond the maximum width of the side of the base plate, making the structure more compact and facilitating the flexible arrangement of multiple base plates. This makes the device more compact overall, with smaller spacing between adjacent base plates and a smaller movement distance of the microscope between different base plates, which facilitates microscope observation and can improve counting efficiency. Attached Figure Description

[0026] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0027] Figure 1 This is a perspective view of the water sample biological counting device of this utility model;

[0028] Figure 2 This is an exploded view of the water sample biological counting device of this utility model;

[0029] Figure 3 This is an exploded view of the base plate (single receiving slot), cover glass, and magnet of this utility model;

[0030] Figure 4 This is a structural schematic diagram of the base plate (single receiving groove) of this utility model;

[0031] Figure 5 This is a schematic diagram of the cover glass of this utility model;

[0032] Figure 6 Exploded view of the base plate (two receiving slots), cover glass and magnet of this utility model;

[0033] Figure 7 This is a schematic diagram of the structure of the base plate (two receiving slots) of this utility model;

[0034] Figure 8 This is a schematic diagram of the installation fixture of this utility model;

[0035] Figure 9 This is a schematic diagram of the fixture frame of this utility model;

[0036] Figure 10 This is a schematic diagram of the card slot structure of this utility model;

[0037] Figure 11 This is a schematic diagram of the slide groove of this utility model;

[0038] Figure 12 This is a schematic diagram of the clip structure of this utility model.

[0039] Explanation of reference numerals in the attached drawings: 1-base plate, 2-cover glass slide, 3-magnet, 4-mounting fixture;

[0040] 11-Outer frame, 111-Overflow groove, 112-Lower recess, 113-Side recess, 114-Partition plate, 115-Focusing hole, 12-Observation platform, 121-Receiving groove, 122-Sealing groove;

[0041] 21-Cover frame, 211-Positioning groove, 212-Protrusion, 22-Glass, 23-Screw;

[0042] 41- Fixture frame, 411- Fixture groove, 412- Slot, 413- Slide groove, 42- Clamp, 421- Sliding part, 422- Pressing groove, 423- Hollowed-out groove, 43- Reset spring. Detailed Implementation

[0043] The technical solutions of this utility model will be clearly and completely described below with reference to the embodiments of this utility model. Obviously, the described embodiments are only a part of the embodiments of this utility model, and not all of them. Based on the embodiments of this utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of this utility model.

[0044] Reference Figure 1-12 As shown in the embodiment of this utility model, a water sample biological counting device is proposed, comprising a base plate 1 and a coverslip 2, wherein:

[0045] The top surface of the base plate 1 is provided with at least one receiving trough 121 for holding water samples. The receiving trough 121 is surrounded by a sealing trough 122. The sealing trough 122 is filled with water samples. An overflow trough 111 is provided around the sealing trough 122. The overflow trough 111 is used to collect water samples that overflow from the receiving trough 121. The base plate 1 includes an outer frame 11 and an observation platform 12. The observation platform 12 is located inside the outer frame 11. The outer frame 11 has a groove cut on the outside of the observation platform 12 to form the overflow trough 111. The receiving trough 121 and the sealing trough 122 are both located on the top surface of the observation platform 12.

[0046] The cover glass 2 is attached to the top surface of the base plate 1 and covers the receiving groove 121 and the sealing groove 122. The cover glass 2 is in contact with the top surface of the observation stage 12, thereby forming sealed chambers with the receiving groove 121 and the sealing groove 122 respectively.

[0047] It should be noted that when only one receiving tank 121 is set on the base plate 1, the volume of the receiving tank 121 can be set to 1ml or 5ml, only the depth of the receiving tank 121 differs. Of course, the volume of the receiving tank 121 can be set according to specific needs, and the volume is the product of the bottom area and the depth of the receiving tank 121. During assembly, the sealing tank 122 is filled with water sample beforehand until the water sample overflows from the sealing tank 122 and reaches the receiving tank 121 and the overflow tank 111. Then, water sample larger than the volume of the receiving tank 121 is injected into the receiving tank 121. The coverslip 2 is placed on the top surface of the base plate 1 to squeeze out the excess water sample and remove the air between the coverslip 2 and the receiving tank 121. The excess water sample overflows into the overflow tank 111. After assembly, it is placed under a microscope for observation, and the organisms in the water sample are counted.

[0048] The water sample biocounting device proposed in this embodiment uses a container 121 to hold water samples. The container 121 is surrounded by a sealing groove 122, which is filled with water samples. An overflow groove 111 is provided around the sealing groove 122 to collect water samples that overflow from the container 121. During evaporation, the water samples in the overflow groove 111 evaporate first, then the water samples in the sealing groove 122 evaporate, and finally the water samples in the container 121 evaporate. By evaporating sequentially from the outside to the inside, the time when the water samples in the container 121 begin to evaporate can be delayed, ensuring that the water samples in the container 121 will not evaporate before the counting is completed, thus improving the accuracy of the counting results. Moreover, compared with the oil sealing method, the cover glass 2 is easy to clean and can be reused until it is no longer usable, resulting in a high reuse rate and significantly reducing costs.

[0049] In some embodiments, the base plate 1 is embedded with a magnet 3, and the cover glass 2 includes a cover frame 21 and a glass 22. The cover frame 21 is made of a magnetic material, and the glass 22 is sealed to the cover frame 21. The visible area of ​​the glass 22 is larger than the area where the receiving groove 121 is located. Threaded holes are provided at the four corners of the cover frame 21. After the glass 22 is placed in the cover frame 21, the threaded holes are connected by screws 23 to complete the connection between the glass 22 and the cover frame 21. The bottom surface of the base plate 1 is provided with a placement hole, in which a magnet 3 is embedded. The magnet 3 can be arranged in accordance with the extension direction of the cover frame 21. When the cover glass 2 is placed on the base plate 1, the magnet 3 magnetically connects to the cover frame 21, so that the cover frame 21 and the base plate 1 are tightly connected, and the receiving groove 121 is sealed. Assembly and disassembly are relatively convenient and quick, which can improve counting efficiency, shorten the counting time, and ensure that the water sample in the receiving groove 121 will not evaporate before the counting is completed. By ensuring that the visible area of ​​the glass 22 is larger than the area of ​​the container 121, it is possible to ensure that the water sample in the entire container 121 can be observed under a microscope, thereby improving the accuracy of counting.

[0050] In some embodiments, the cover frame 21 has a positioning groove 211 on the side facing the receiving groove 121, and the observation platform 12 is nested in the positioning groove 211. The positioning groove 211 is aligned with the observation platform 12 and fitted onto it; the magnetic attraction of the magnet 3 completes the quick connection. During the process of squeezing out excess water from the glass 22, the cover frame 21 will not drift, making positioning convenient, quick, and accurate. This allows the entire receiving groove 121 to be visible from the position of the glass 22, improving installation efficiency. Furthermore, compared to manual alignment by visual observation, the success rate is higher, improving assembly efficiency and thus increasing counting efficiency.

[0051] In some embodiments, the top surface of the base plate 1 has a recessed portion 112 along the periphery of the overflow groove 111, and the two sides of the cover frame 21 have protrusions 212 extending outward. The protrusions 212 are placed in the recessed portion 112 and protrude from the side of the base plate 1. The outer frame 11 has recessed portions 112 on both sides along its width direction, and the two sides of the cover frame 21 have protrusions 212 extending outward. When the cover frame 21 is closed on the base plate 1, the protrusions 212 are placed in the recessed portion 112 and protrude from the side of the outer frame 11, making it easier to put and take off the cover frame 21 through the protrusions 212, making the operation more convenient, improving the assembly and disassembly efficiency, and thus improving the counting efficiency.

[0052] In some embodiments, the side of the base plate 1 is provided with a side recess 113 corresponding to the position of the recess 112, and the protrusion 212 protrudes from the inner sidewall of the side recess 113. The protrusion 212 may not extend beyond the maximum width of the side of the outer frame 11, making the structure more compact, facilitating the flexible arrangement of multiple base plates 1, making the overall device more miniaturized, reducing the spacing between adjacent base plates 1, and decreasing the distance the microscope moves between different base plates 1, thus facilitating microscope observation and improving counting efficiency.

[0053] In some embodiments, such as Figure 6 and Figure 7 As shown, there are two receiving slots 121, two sealing slots 122, and two overflow slots 111. There are also two cover slips 2, each covering slip 2 covering one receiving slot 121. When the volume of the receiving slot 121 is small (e.g., 0.1 ml), two receiving slots 121 can be provided on each base plate 1. The base plate 1 includes an outer frame 11 and two observation stages 12. A partition plate 114 can be provided in the middle of the outer frame 11 to divide the outer frame 11 into two independent spaces. The two receiving slots 121 and the two sealing slots 122 are respectively provided on the corresponding observation stages 12. The overflow slot 111 is located outside the corresponding sealing slot 122 and between the observation stage 12 and the outer frame 11.

[0054] In other embodiments, in actual design, the outer frame 11 may not have a partition plate 114, and the two overflow channels 111 may be integrated as a whole.

[0055] In some embodiments, the top surface of the base plate 1 is provided with focusing holes 115 at both ends along the length direction. The focusing holes 115 are located at both ends of the outer frame 11 along the length direction and at the midpoint of the width direction of the outer frame 11, which facilitates the focusing of the microscope and improves the efficiency of observation and counting.

[0056] In some embodiments, the water sample biological counting device further includes a mounting fixture 4, which has multiple clamping stations for clamping the base plate 1. By clamping the base plate 1 at the clamping stations on the mounting fixture 4, and then mounting the mounting fixture 4 on the observation stage 12 of the microscope, multiple base plates 1 can be clamped. A single clamping operation can be used to observe and count water samples on multiple base plates 1, improving counting efficiency.

[0057] In some embodiments, the mounting fixture 4 includes a fixture frame 41 and a clamp 42. The fixture frame 41 has a plurality of fixture slots 411. One side of each fixture slot 411 has a slot 412, and the clamp 42 is mounted on the other side. The clamp 42 is slidably mounted on the fixture frame 41. A return spring 43 is provided between the clamp 42 and the inner wall of the fixture frame 41. The fixture frame 41 has a sliding groove 413. The clamp 42 has sliding portions 421 on both sides, which are slidably mounted in the sliding groove 413. The slot 412 is inclined from the inside to the outside from top to bottom. One end of the outer frame 11 abuts against the slot 412. The lower part of the clamp 42 has a pressing groove 422, which abuts against the top and side surfaces of the other end of the outer frame 11. The return spring 43 clamps the outer frame 11 between the clamp 42 and the slot 412. During installation, an external force is applied to move the outer frame 11 toward one side of the clamp 42 to compress the return spring 43, placing the outer frame 11 in the fixture slot 411. Then, the outer frame 11 is released, and under the elastic force of the return spring 43, the outer frame 11 moves toward the opposite slot 412, with the other side of the outer frame 11 abutting against the slot 412, thus clamping the base plate 1. During disassembly, only an external force needs to be applied to move the outer frame 11 toward one side of the clamp 42 to remove the assembly of the base plate 1 and the cover glass 2. Installation and disassembly are relatively convenient and quick, which can improve counting efficiency.

[0058] In some embodiments, the top surface of the clip 42 is provided with a hollowed-out groove 423, which facilitates the application of external force by hand to the clip 42, thereby improving the convenience of operation and the efficiency of assembly and disassembly.

[0059] In some embodiments, the receiving groove 121 has a rectangular cross-section. A rectangular cross-section of the receiving groove 121 facilitates volume calculation, makes it easier to design the bottom area as an integer, and facilitates depth design for receiving grooves 121 with different volumes.

[0060] The working principle of this water sample biological counting device is as follows: the sealing groove 122 is filled with water sample beforehand until the water sample overflows from the sealing groove 122 and reaches the receiving groove 121 and the overflow groove 111. Then, water sample larger than the volume of the receiving groove 121 is injected into the receiving groove 121. The positioning groove 211 is placed on the observation table 12. The magnet 3 attracts the cover frame 21, so that the cover glass 2 covers the top surface of the base plate 1, squeezing out the excess water sample and expelling the air between the glass 22 and the receiving groove 121. The excess water sample overflows into the overflow groove 111. After assembly, it is placed under a microscope for observation to count the organisms in the water sample. During evaporation, the water sample in the overflow tank 111 evaporates first, then the water sample in the sealing tank 122 evaporates, and finally the water sample in the receiving tank 121 evaporates. By evaporating sequentially from the outside to the inside, the time when the water sample in the receiving tank 121 begins to evaporate can be delayed, ensuring that the water sample in the receiving tank 121 will not evaporate before the counting is completed, thus improving the accuracy of the counting results. Moreover, compared with the oil sealing method, the cover glass 2 is easy to clean and can be reused until it is no longer usable, with a high reuse rate, which can significantly reduce costs.

[0061] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A device for counting aquatic organisms, characterized in that The device comprises a base plate and a cover glass, wherein: a top surface of the base plate is provided with at least one holding groove for holding water sample, a sealing groove is arranged around the holding groove, the sealing groove is filled with water sample, an overflow groove is arranged around the sealing groove, and the overflow groove is used for collecting water sample overflowing from the holding groove; the cover glass is connected to the top surface of the base plate and covers the holding groove and the sealing groove.

2. The apparatus for counting aquatic organisms of claim 1, wherein The base plate is embedded with a magnet, the cover glass comprises a cover frame and a glass, the cover frame is made of magnetic material, the glass is sealingly connected to the cover frame, and a visible area of the glass is larger than an area where the holding groove is located.

3. The apparatus for counting aquatic organisms of claim 2, wherein, The base plate comprises an outer frame and an observation platform, the observation platform is located inside the outer frame, the outer frame is recessed outside the observation platform to form the overflow groove, and the holding groove and the sealing groove are both located on a top surface of the observation platform. One side of the cover frame facing the holding groove is provided with a positioning groove, and the observation platform is nested in the positioning groove.

4. The apparatus for counting aquatic organisms of claim 2, wherein The top surface of the base plate is provided with a lower recess along a periphery of the overflow groove, both sides of the cover frame are provided with a protruding part outward, the protruding part is placed in the lower recess, and the protruding part protrudes from a side surface of the base plate.

5. The apparatus for counting aquatic organisms of claim 4, wherein, A side recess is arranged on a side surface of the base plate corresponding to a position of the lower recess, and the protruding part protrudes from an inner side wall of the side recess.

6. The apparatus of claim 1 wherein, The holding groove is provided with two, the sealing groove and the overflow groove are correspondingly provided with two, the cover glass is provided with two, and each cover glass corresponds to one holding groove.

7. The apparatus of claim 1 wherein, The top surface of the base plate is provided with a focusing hole at both ends in a length direction.

8. The apparatus for counting aquatic organisms of claim 1, wherein The device further comprises a mounting jig, the mounting jig is provided with a plurality of clamping stations, and the clamping stations are used for clamping the base plate.

9. The apparatus for counting aquatic organisms of claim 8, wherein, The mounting jig comprises a jig frame and a clamp, the jig frame is provided with a plurality of jig grooves, one side of the jig groove is provided with a clamping groove, and the other side is provided with the clamp, the clamp is slidingly installed on the jig frame, and a return spring is arranged between the clamp and an inner wall of the jig frame.

10. The device for counting aquatic organisms according to any one of claims 1 to 9, wherein The holding groove has a rectangular cross section.

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