Auxiliary device for fixing small-capacity collecting pipe of flow sorter
By designing an auxiliary device for fixing small-capacity collection tubes in a flow cytometer, the problem of existing devices being unable to stably fix small-capacity collection tubes was solved, enabling stable collection and multi-channel sorting of rare cells, and improving the accuracy and success rate of experiments.
Patent Information
- Application Number
- CN202520277093.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-20
- Publication Date
- 2026-01-16
- Estimated Expiration
- 2035-02-20
AI Technical Summary
Existing flow cytometer collection devices cannot stably fix small-capacity collection tubes, resulting in excessively low cell concentrations during rare cell sorting, which affects the accuracy and reliability of experimental results.
Design an auxiliary device for fixing small-capacity collection tubes in a flow classifier, including an insert tube and a collection tube placement cavity. The outer diameter of the insert tube is larger than the inner diameter of the adapter groove, and it has a guide strip to ensure stable placement and centered positioning of the small-capacity collection tube.
This method achieves stable fixation of small-capacity collection tubes, reduces cell loss, improves the efficiency of multi-channel collection of rare cells, and ensures the accuracy and success rate of experimental results.
Smart Images
Figure CN223805073U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to cell sorting equipment technical field, concretely relates to a kind of for the auxiliary device for fixing of small capacity collection tube of flow sorting instrument. BACKGROUND
[0002] The collection device of the MofloAstrios EQ flow sorting instrument of BECKMAN company includes 1.5mL EP tube, 5mL flow tube and 96, 384 hole plate sorting device. The capacity of sorting collection device is at least 1.5mL, and for some rare (rare) cell sorting, using the sorting collection device to collect can cause the cell concentration to be too low, affect subsequent experiment, for example, cell is difficult to identify and count, and for example, in subsequent experimental operation, such as centrifugation, liquid exchange, low-concentration cell is more easily lost or lost, to further reduce the number of cells, to affect the accuracy and reliability of experimental results. Therefore, when carrying out cell sorting, appropriate receiving container capacity should be selected according to the number of cells and experimental requirements, to ensure that the cell concentration is moderate, to improve the success rate of sorting efficiency and subsequent experiment.
[0003] The collection device adapter of existing flow sorting instrument is generally suitable for 5mL flow tube and 1.5mL EP tube, as shown in Figure Figure 1 When using 5mL flow tube, flow tube can be directly inserted into the groove of adapter, and 1.5mL EP can also be inserted into the groove of adapter, and is hung along the tube at the mouth of groove. And for smaller capacity, such as 0.2mL, 0.3mL, 0.5mL collection tube, its outer diameter is much smaller than the inner diameter of groove, and when placed in the groove, it will fall to the bottom of the groove, and cannot be effectively collected for cell sorting. Although 96 hole plate can be used for collection, only one-way cell can be collected, and multiple sorting cannot be carried out, resulting in cell waste, especially when rare cell sorting is carried out, the collection device cannot meet the needs. Therefore, for the rare cell sorting requirement of small capacity collection tube, it is necessary to design an auxiliary device to help small capacity collection tube to be stably fixed on the upper part of the groove of adapter. SUMMARY
[0004] To solve the above problems in the prior art, the utility model provides an auxiliary device for fixing small capacity collection tube of flow sorting instrument.
[0005] To achieve the above technical purpose, the utility model adopts the technical scheme that:
[0006] An auxiliary device for fixing small capacity collection tube of flow sorting instrument, comprising a tube, a collection tube placing cavity is arranged in the tube, the tube comprises a tube body and a tube edge, the tube edge is located at the outer periphery of the top of the opening side of the tube body and extends outward, and the outer diameter of the tube edge is greater than the inner diameter of the groove of the adapter.
[0007] As a preferred technical solution, the outer wall of the tube body of the cannula is in the shape of an inverted cone or an inverted truncated cone.
[0008] As a preferred technical solution, the collecting tube placement cavity is in the shape of a cylinder or an inverted truncated cone,
[0009] As a preferred technical solution, the maximum outer diameter of the tube body is equivalent to the inner diameter of the adapter groove.
[0010] As a preferred technical solution, the cannula is made of transparent glass or plastic material.
[0011] As another preferred technical solution, the tube body of the cannula is in the shape of a thin-walled cylinder, and the outer wall of the tube body is in the shape of a cylinder.
[0012] As a preferred technical solution, the outer wall of the tube body is uniformly provided with a plurality of guide strips in the circumferential direction, the guide strips are arranged in the radial direction of the tube body, and the distance from the outermost edge of the guide strip to the center of the tube body is equivalent to the radius of the adapter groove.
[0013] As a preferred technical solution, the guide strips are provided in four, and the four guide strips are arranged at an interval of 90° in the circumferential direction of the tube body.
[0014] As a preferred technical solution, the side of the guide strip away from the center of the tube body is in an inclined shape, and the distance from the outer edge of the guide strip to the center of the tube body decreases from the top to the bottom of the tube body.
[0015] As a preferred technical solution, the cannula is made of plastic material.
[0016] The beneficial effects of the utility model are as follows:
[0017] The auxiliary device for fixing the small-capacity collecting tube of the flow cytometer can place the small-capacity collecting tube in the collecting tube placement cavity in the cannula, such as 0.2mL, 0.3mL and 0.5mL collecting tubes, and the cannula can be placed in the groove of the adapter, so that the stable placement of the small-capacity collecting tube is met, the collection of rare cells is realized, the loss of cells is reduced, and the yield is improved.
[0018] The auxiliary device for fixing the small-capacity collecting tube of the flow cytometer can play a central positioning role in the shape of the cannula, and ensure that the center of the collecting tube placement cavity (i.e. the small-capacity collecting tube) is aligned with the center of the adapter groove.
[0019] The auxiliary device is a separate individual, is used on the collection pipe adapter of the flow sorting instrument, can be placed in the corresponding groove of the adapter according to needs, so that the small-capacity collection pipe is placed, and the multi-way collection of rare cells is realized, and 0.2mL EP pipes, 1.5mL EP pipes and 5mL flow pipes can be mixed and sorted, so that the collection device is more personalized. BRIEF DESCRIPTION OF DRAWINGS
[0020] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed in the embodiments will be briefly introduced as follows, and it should be understood that the following drawings only show some embodiments of the present application, and should not be regarded as a limitation on the scope, and for those skilled in the art, other related drawings can also be obtained without creative labor on the basis of these drawings.
[0021] Figure 1 is a schematic view of the collection device adapter of the flow sorting instrument in the prior art;
[0022] Figure 2 is a structural schematic view of the embodiment of the present application;
[0023] Figure 3 is a structural schematic view of another embodiment of the present application.
[0024] The drawings show that: 1 is a cannula, 2 is a collection pipe placing cavity, 3 is a pipe body, 4 is a pipe edge, and 5 is a guide strip. DETAILED DESCRIPTION
[0025] In order to make the purpose, technical scheme and advantages of the embodiments of the present application more clear, the technical scheme in the embodiments of the present application will be clearly and completely described below in combination with the drawings in the embodiments of the present application, and obviously, the described embodiments are some embodiments of the present application, not all the embodiments. The components of the embodiments of the present application described and shown in the drawings here can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the present application provided in the drawings is not intended to limit the scope of the claimed present application, but only represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.
[0026] An auxiliary device for fixing a small-capacity collection pipe of a flow sorting instrument, as shown in Figure 2 , Figure 3As shown, including the cannula 1, the cannula 1 is provided with a collection tube placement cavity 2, the cannula 1 includes a tube body 4 and a tube edge 3, the tube edge 3 is located at the outer periphery of the top of the opening side of the tube body 4 and extends outward, the outer diameter of the tube edge 3 is greater than the inner diameter of the adapter groove, and the cannula 1 is clamped on the top of the adapter groove through the tube edge 3.
[0027] In one embodiment, the outer wall of the tube body 4 of the cannula is in the shape of an inverted cone or an inverted truncated cone, the maximum outer diameter of the tube body 4 is equivalent to the inner diameter of the adapter groove, and the collection tube placement cavity 2 is in the shape of a cylinder or an inverted truncated cone, and the small-capacity collection tube is placed in the collection tube placement cavity 2. Preferably, the cannula in this embodiment is made of transparent glass or plastic material.
[0028] In another preferred embodiment, the tube body 4 of the cannula is in the shape of a thin-walled cylinder, the outer wall of the tube body 4 is in the shape of a cylinder, and a plurality of guide strips 5 are uniformly arranged on the outer wall of the tube body 4 in the circumferential direction, the guide strips 5 are arranged in the radial direction of the tube body 4, and the distance from the outermost edge of the guide strips 5 to the center of the tube body 4 is equivalent to the radius of the adapter groove. Preferably, the guide strips 5 are provided in four, the four guide strips 5 are arranged in the circumferential direction of the tube body 4 at intervals of 90°, the side of the guide strips 5 away from the center of the tube body 4 is in the shape of an inclination, and the distance from the outer edge of the guide strips 5 to the center of the tube body 4 decreases from the top to the bottom of the tube body 4. Preferably, the cannula in this embodiment is made of plastic material.
[0029] The auxiliary device of the utility model, the collection tube placement cavity in the cannula is used for placing the small-capacity collection tube, and the cannula can be placed in the groove of the adapter, thereby meeting the stable placement of the small-capacity collection tube. The shape of the cannula can play a role in center positioning, and the center of the collection tube placement cavity (i.e. the small-capacity collection tube) is aligned with the center of the adapter groove.
[0030] The auxiliary device of the utility model is a separate individual, which is used on the collection tube adapter of the flow cytometer, and can be placed in the corresponding groove of the adapter as needed, thereby placing the small-capacity collection tube, realizing the multi-channel collection of rare cells, and also realizing the mixed sorting of 0.2mL EP tubes, 1.5mL EP tubes and 5mL flow tubes, so that the collection device is more personalized.
[0031] Of course, the utility model can also have other various embodiments, and those skilled in the art can make various corresponding changes and modifications according to the utility model without departing from the spirit and essence of the utility model, but these corresponding changes and modifications should belong to the protection scope of the claims attached to the utility model.
Claims
1. An aid for securing a flow cytometer small volume collection tube, comprising: The invention relates to a catheter (1) comprising a collecting tube placing cavity (2) inside the catheter (1), the catheter (1) comprising a tube body (4) and a tube rim (3), the tube rim (3) being located at the top outer periphery of the opening side of the tube body (4) and extending outward, the outer diameter of the tube rim (3) being larger than the inner diameter of the adapter groove.
2. An auxiliary device for flow cytometer low volume collection tube fixation according to claim 1, wherein: The outer wall of the tube body (4) of the catheter is in the shape of an inverted cone or an inverted truncated cone.
3. An auxiliary device for flow cytometer low volume collection tube fixation according to claim 2, wherein: The collecting tube placing cavity (2) is in the shape of a cylinder or an inverted truncated cone.
4. The supplemental device for flow sorter small volume collection tube immobilization of claim 2, wherein: The maximum outer diameter of the tube body (4) is equivalent to the inner diameter of the adapter groove.
5. The auxiliary device for fixing small volume collection tubes of a flow sorter according to any one of claims 2 to 4, characterized in that: The catheter is made of transparent glass or plastic material.
6. An auxiliary device for flow sorter small volume collection tube fixation according to claim 1, characterized in that: The tube body (4) of the catheter is in the shape of a thin-walled cylinder, and the outer wall of the tube body (4) is in the shape of a cylinder.
7. An auxiliary device for flow cytometer low volume collection tube fixation according to claim 6, wherein: A plurality of guide strips (5) are uniformly arranged on the outer wall of the tube body (4) in the circumferential direction, the guide strips (5) being arranged in the radial direction of the tube body (4), and the distance from the outermost edge of the guide strips (5) to the center of the tube body (4) is equivalent to the radius of the adapter groove.
8. An auxiliary device for flow cytometer low volume collection tube fixation according to claim 7, wherein: The guide strips (5) are arranged in the circumferential direction of the tube body (4) at an interval of 90°.
9. The supplemental device for flow sorter small volume collection tube immobilization of claim 7, wherein: The side of the guide strips (5) away from the center of the tube body (4) is in the shape of an inclination, and the distance from the outer edge of the guide strips (5) to the center of the tube body (4) decreases from the top to the bottom of the tube body (4).
10. The auxiliary device for the fixation of small volume collection tubes in a flow sorter according to any one of claims 6 to 9, characterized in that: The catheter is made of plastic material.