Blood type percolation detection card

Abstract

The utility model provides a blood type percolation detection card which can be used for identifying the blood type of a person or an animal and comprises an upper shell, a lower shell and a test strip, the upper shell is provided with a sample adding hole, the lower shell is provided with a limiting groove, the test strip is fixed in the limiting groove, a protrusion is arranged in the limiting groove, and the protrusion can jack up the test strip to prevent the test strip from making contact with the lower shell and is used for accelerating the capillary action, so that the result obtaining speed is increased; the clinging degree of the test strip and the sample adding hole can be adjusted; the periphery of the sample adding hole is provided with an elliptical recess for storing a dropped blood sample or flushing fluid; a bulge is also arranged on the back surface of the elliptical recess and is used for reinforcing and fixing the test strip. The device has the advantages of simplicity in manufacturing, small size, light weight, low price, short detection time and accurate detection result, and is suitable for large-scale detection.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the blood type detection field, specifically, relate to a blood type filtration detection card. BACKGROUND

[0002] The blood type system of animals varies by species, and most mammals have a blood type system. Blood type refers to a genetic trait that is manifested in the form of blood antigens and classified according to the presence or absence of certain antigenic substances on the surface of red blood cells.

[0003] Currently, 30 human blood type systems have been discovered and recognized by the International Blood Transfusion Association, such as ABO, Rh, MNS, and P blood type systems, and the most important are the ABO and Rh (Rhesus monkey factor) blood type systems. The ABO blood type system is the earliest recognized blood type system in humans discovered by Karl Landsteiner. According to whether the red blood cell membrane surface contains A or B agglutinogen, the blood type system is divided into A type, B type, AB type, and O type. The Rh blood type system is the most polymorphic blood type system in humans, which contains more than 45 antigens, and the most closely related antigens in clinical practice are five: D, E, C, c, and e. The D antigen in this antigen is the strongest and most important in clinical practice. According to whether the human red blood cells contain D antigen, the blood type system is divided into Rh positive and Rh negative.

[0004] In recent years, more and more people keep pets, and blood type detection is often performed on pets in pet medical diagnosis. Taking the most common pet cats and dogs as examples, the blood type of cats is mainly divided into three types: A type, B type, and AB type. Current research shows that dogs have 13 blood types, but only 8 of them meet international standards, which are DEA1.1, DEA1.2, DEA3, DEA4, DEA5, DEA6, DEA7, and DEA8.

[0005] Blood transfusion, as an effective treatment method, has been widely used in clinical or pet medical treatment. Taking humans as an example, about 85 million units of blood are transfused into patients worldwide each year. However, blood transfusion also has certain risks. If the transfused blood is incompatible with the patient, acute hemolytic reaction may occur, causing shock and renal failure, and even death. If blood type compatibility detection is not performed before blood transfusion, about 1 / 3 of patients may experience hemolytic reaction. Therefore, rapid and accurate ABO / Rh blood type detection is particularly important for patients who need blood transfusion. Correct identification of ABO / Rh blood type of blood donors and recipients is an essential prerequisite for ensuring blood transfusion safety. The same is true for pet blood transfusion. If the blood types do not match, it may trigger a serious transfusion reaction and even endanger the life of the pet.

[0006] The basic principle of blood group detection method is the agglutination reaction of red blood cell antigen and serum antibody. The classification of blood group depends on whether there is corresponding antigen on the red blood cell membrane. According to Landsteiner's law, if the red blood cell membrane contains a certain antigen (such as human A, B or D antigen), the serum does not contain the corresponding antibody; on the contrary, if the red blood cell surface lacks a certain antigen, the serum contains the corresponding antibody.

[0007] The commonly used blood group detection methods in clinic include slide method, test tube method and micro-column gel method, etc. The above methods are accurate, objective, and have high sensitivity and specificity, but still have some shortcomings: (1) professional operation is required; (2) specific experimental instruments are required; (3) appropriate experimental conditions are required; (4) the reaction time is relatively long; (5) more blood is required; (6) the sample needs pre-treatment. Therefore, although the traditional blood group detection method can meet the needs of clinic and laboratory, it cannot meet the mass blood group detection under special circumstances such as war environment, major natural disasters, economically backward underdeveloped areas, etc. Therefore, the point-of-care testing (POCT) method which is cheap, simple and fast, easy to carry, intuitive and easy to interpret is very important for blood group detection under special circumstances.

[0008] As a new development direction of medical examination in the 21st century, POCT has been paid more and more attention. Its main characteristics are "fast, side, convenient and easy", that is, outside the traditional laboratory, non-laboratory professionals use the simplest and most economical method to obtain accurate results in the shortest time. In the draft of "POCT Special Certificate" formulated by American Clinical Biochemistry Society, POCT is defined as "clinical laboratory examination conducted by clinical personnel who have not received multi-disciplinary training in clinical laboratory or patients (self-examination) near the patient treatment site".

[0009] The existing blood group filtration detection card directly contacts the test strip with the lower shell, so that the air pressure on the upper and lower sides of the test strip is not circulated, and the sample to be tested is blocked by the capillary effect and penetrates into the test strip. Such a structure design not only makes the detection time longer, but also increases the possibility of detection failure; in addition, it also causes the test strip to be too concave, and the distance from the sample addition hole is too far, which will cause the detection result to be inaccurate. In addition, many blood group filtration detection cards fix the test strip only in the lower shell design card slot and limiting plate, and do not set limiting device in the upper shell, which will cause the test strip to displace during the detection process, so as to cause inaccurate or failed detection results, and the test strip will be damaged due to displacement and collision between the upper shell and the lower shell during transportation.

[0010] Therefore, it is urgent to find a blood group filtration detection card to solve the above problems. Content of the utility model

[0011] The utility model provides a blood type infiltration detection card, can identify the blood type of people or animals, including upper casing, lower casing and test strip, the upper casing sets up sample adding hole, the lower casing sets up limit groove, the test strip is fixed in the limit groove, the limit groove is provided with the protrusion, the protrusion can not only lift the test strip to avoid the test strip and lower casing contact for accelerating capillary action, and then accelerate the speed of result, can also adjust the close degree of test strip and sample adding hole, the periphery of sample adding hole is provided with oval recess for storing droped blood sample or irrigation fluid, the back of oval recess is also provided with protrusion for strengthening the test strip.

[0012] A blood type infiltration detection card, including upper casing, lower casing and test strip, the upper casing sets up sample adding hole, the lower casing sets up limit groove, the limit groove is provided with first protrusion, the protrusion can not only lift the test strip to avoid the test strip and lower casing contact, can also adjust the close degree of test strip and sample adding hole.

[0013] On the one hand, the test strip is infiltrated by the capillary action of the sample to be measured, and the protrusion in the limit groove lifts the test strip, so that there is a gap between the test strip and the lower casing. Therefore, the air pressure flows through the upper and lower surfaces of the test strip, accelerates the capillary action of the test strip, and quickly displays the detection result. On the other hand, the protrusion in the limit groove lifts the test strip to adjust the distance between the test strip and the sample adding hole to the optimal position. If the test strip is too recessed in the limit groove, the distance between the test strip and the sample adding hole will be too large, which will lead to inaccurate detection results and damage to the test strip during transportation. Therefore, the protrusion in the limit groove not only fixes and protects the test strip, but also accelerates the detection speed and improves the accuracy of the detection result.

[0014] Further, the upper casing is provided with an oval recess, and the sample adding hole is located in the middle of the oval recess.

[0015] The oval recess is used for temporarily storing the added sample to be measured and the irrigation fluid, provides a buffer space for capillary action, allows the sample to be measured to react more fully with the test strip, and allows the irrigation fluid to be washed more cleanly.

[0016] Further, the back of the oval recess is provided with a second protrusion.

[0017] The back of the oval recess has two second protrusions, which can resist the infiltration membrane to strengthen the fixation of the infiltration membrane, align the sample adding hole with the infiltration membrane, and prevent displacement.

[0018] Further, the limit groove is provided with limit plates at both ends and both sides.

[0019] The two end limiting plates prevent the test strip from moving forward and backward, and the two side limiting plates prevent the test strip from moving left and right.

[0020] Further, the limiting plates arranged on both sides of the limiting groove include horizontal limiting plates and vertical limiting plates.

[0021] The horizontal limiting plates are perpendicular to the limiting groove and are arranged at a position of two-thirds from the top to the bottom of the limiting groove; and the vertical limiting plates are parallel to the limiting groove and are arranged at a position of one-third from the top to the bottom of the limiting groove.

[0022] Further, the test strip includes a filter membrane, an absorbent paper and a fixing plate; the filter membrane and the fixing plate are arranged on the absorbent paper, the filter membrane is located in the middle of the absorbent paper, and the fixing plate is located on both sides of the filter membrane.

[0023] The filter membrane is composed of fibers, and the small holes in the filter membrane have capillary action, so that no external energy is needed. Therefore, the filter membrane is selected as the material of the test strip for detecting blood type. The diameters of red blood cells of different animals are different. For example, the diameter of human red blood cells is in the range of 6-9 μm, the diameter of cat red blood cells is about 5.5-6 μm, and the diameter of dog red blood cells is about 7 μm. Therefore, for red blood cells of different species, a filter membrane with a suitable size should be selected, so that the pore size of the filter membrane is larger than the diameter of the red blood cells but smaller than the diameter of the agglutinated red blood cell aggregates. The filter membrane is composed of three layers. The uppermost filter membrane is coated with an antibody. When the red blood cells come into contact with the filter membrane, the red blood cells will sink due to the capillary action of the pores of the filter membrane. If the red blood cells and the antigen coated on the uppermost filter membrane have an agglutination reaction, the diameter of the agglutinated red blood cells will increase and exceed the pore size of the filter membrane, so the agglutinated red blood cells will not sink. The filter membrane has the advantages of low price, biodegradability, easy disposal, lightness and easy portability.

[0024] The filter membrane is the main reaction area. The sample to be tested sinks through the filter membrane and is finally absorbed by the absorbent paper at the bottom. The liquid will gradually penetrate to both sides of the absorbent paper and be absorbed by the absorbent paper.

[0025] Further, the upper surface of the fixing plate is provided with adhesive tape, and one end of the fixing plate close to the filter membrane is fixed to the filter membrane by the adhesive tape.

[0026] The fixing plate is made of a fixing material, and the fixing plate is located on both sides of the filter membrane. The lower surfaces of the two fixing plates are fixed to the absorbent paper, and the upper surfaces of the two fixing plates are provided with adhesive tape. The adhesive tape can stick to both sides of the upper surface of the filter membrane, so that the filter membrane can be fixed in the middle of the absorbent paper without displacement.

[0027] Further, the filter membrane includes a first filter membrane, a second filter membrane and a third filter membrane, and the first filter membrane is coated with an antibody.

[0028] The antibodies include all antibodies corresponding to the specific antigens on the surface of animal red blood cells identifying their blood types, such as human anti-A monoclonal antibodies, anti-B monoclonal antibodies, anti-D monoclonal antibodies, cat anti-A monoclonal antibodies, anti-B monoclonal antibodies, anti-AB monoclonal antibodies, and the like, dog anti-DEA1.1 monoclonal antibodies, anti-DEA1.2 monoclonal antibodies, anti-DEA3 monoclonal antibodies, anti-DEA4 monoclonal antibodies, anti-DEA5 monoclonal antibodies, anti-DEA6 monoclonal antibodies, anti-DEA7 monoclonal antibodies, and anti-DEA8 monoclonal antibodies, and the like.

[0029] The first diafiltration membrane is coated with antibodies as a solid phase carrier. When the sample is in contact with the diafiltration membrane, if the red blood cell surface antigen in the sample and the antibody in the solid phase carrier have an immunosorbent reaction, the red blood cells will remain on the first diafiltration membrane. After washing with the washing solution, the red blood cells will not sink due to the agglutination reaction, so the first diafiltration membrane shows a blood red color, which is a positive reaction. On the contrary, if the red blood cells do not have an agglutination reaction, they will sink along the order of the first diafiltration membrane, the second diafiltration membrane, and the third diafiltration membrane from top to bottom after washing with the washing solution, so the first diafiltration membrane shows no color, which is a negative reaction.

[0030] In some ways, the blood group diafiltration detection card is used to detect human ABO / Rh blood groups. According to the blood group system used, three sample wells are set. The first sample well from left to right detects A blood group, the first diafiltration membrane of which is coated with anti-A monoclonal antibodies containing red dye. The second sample well detects B blood group, the first diafiltration membrane of which is coated with anti-B monoclonal antibodies containing yellow dye. The third sample well detects Rh blood group, the first diafiltration membrane of which is coated with anti-D monoclonal antibodies containing green dye. Red, yellow, and green are the most easily distinguishable typical colors with the naked eye, so these three colors of dye are selected as the identifiers for different antibodies. In addition, coating the first diafiltration membranes with different monoclonal antibodies in different colors can avoid the possibility of confusion during the manufacture of the test strip.

[0031] Further, the diafiltration membranes are stacked in the order of the first diafiltration membrane, the second diafiltration membrane, and the third diafiltration membrane from top to bottom.

[0032] Further, the number of test strips includes one or more, the number of limiting grooves includes one or more, the number of sample wells includes one or more, the lower shell is further provided with a jack, and the upper shell is further provided with a jack.

[0033] According to the blood type system of the animal to be used, the corresponding number of sample adding holes, test strips and limiting grooves are arranged, and the filtration membrane of each test strip is coated with different antibodies in the blood type system to be used.

[0034] In some ways, the inner side of the edge of the lower shell is further provided with six insertion holes, and the inner side of the edge of the upper shell is further provided with six insertion pins, and the insertion holes and the insertion pins correspond.

[0035] The utility model has the following beneficial effects:

[0036] (1) provide a blood type filtration detection card, can detect the blood type of human, cat, dog and the like, including upper shell, lower shell and test strip;

[0037] (2) the upper shell is provided with sample adding hole, the lower shell is provided with limiting groove, the test strip is fixed in the limiting groove, the limiting groove is provided with protrusion, the protrusion not only can lift the test strip and avoid the contact of test strip and lower shell, is used for accelerating capillary action, and then accelerates the speed of result, can also adjust the close degree of test strip and sample adding hole;

[0038] (3) the oval recess around the sample adding hole is used for storing the drop of blood sample or flushing fluid;The back of the oval recess is further provided with a protrusion for strengthening the fixation of the test strip to prevent displacement.

[0039] (4) the utility model has the advantages of simple production, small and light, low price, and is suitable for large batch detection, short detection time and accurate detection result. BRIEF DESCRIPTION OF DRAWINGS

[0040] Figure 1 It is the overall structure diagram of human blood type filtration detection card;

[0041] Figure 2 It is the structure diagram of lower shell;

[0042] Figure 3 It is the structure diagram of test strip in limiting groove;

[0043] Figure 4 It is the structure diagram of test strip;

[0044] Figure 5 It is the top view of upper shell;

[0045] Figure 6 It is the bottom view of upper shell;

[0046] Figure 7Structure diagram of setting two limiting grooves in the lower shell;

[0047] Figure 8 Structure diagram of fixing two test paper strips by setting two limiting grooves in the lower shell;

[0048] Figure 9 Top view of setting two sample adding holes in the upper shell;

[0049] Figure 10 Bottom view of setting two sample adding holes in the upper shell. DETAILED DESCRIPTION

[0050] The utility model will be further described in detail below in combination with the drawings and examples, and it should be pointed out that the following examples are intended to facilitate the understanding of the utility model and do not limit the utility model.

[0051] Example 1, a blood group filtration detection card

[0052] This example takes detecting human ABO / Rh blood group as an example and provides a human blood group filtration detection card.

[0053] It should be noted that the first filtration membrane of the filtration membrane can be coated with antibodies corresponding to specific antigens on the red blood cells of other animals for identifying the blood group of other animals, and the corresponding number of sample adding holes, test paper strips and limiting grooves for fixing the test paper strips can be set according to the blood group system adopted.

[0054] As shown in Figure 1 , it is a human blood group filtration detection card, which comprises a lower shell 1, a test paper strip 2 and an upper shell 3.

[0055] As shown in Figures 1-2As shown, the lower shell 1 is provided with three identical limiting grooves 4, i.e. limiting groove 5, limiting groove 6 and limiting groove 7, for fixing the test paper strip 2. The first protrusion 8 is arranged in each of the limiting groove 5, limiting groove 6 and limiting groove 7, which will lift the test paper strip 2. On the one hand, the first protrusion 8 avoids the contact between the test paper strip 2 and the lower shell 1, the main reaction zone of the test paper strip 2 is mainly composed of the filtration membrane, and the first protrusion 8 makes the test paper strip 2 have a gap with the lower shell 1, so that the air pressure flows through the upper and lower surfaces of the test paper strip 2, accelerates the capillary action of the test paper strip 2, and can accelerate the display of the detection result. On the other hand, the first protrusion 8 adjusts the distance between the test paper strip 2 and the sample adding hole to the optimal position. If the test paper strip 2 is too recessed in the limiting groove, the distance between the test paper strip 2 and the sample adding hole 23 will be too large, which will affect the detection result, and the test paper strip 2 may be damaged during the transportation process due to the up and down displacement. The limiting groove 5, limiting groove 6 and limiting groove 7 are provided with limiting plates 9 at both ends, so that the test paper strip 2 will not displace forward and backward. The limiting groove is provided with longitudinal limiting plates 10 and transverse limiting plates 11 at both sides, so that the test paper strip 2 will not displace left and right. The longitudinal limiting plates 10 are parallel to the limiting groove, and the transverse limiting plates 11 are perpendicular to the limiting groove. The inner side of the edge of the lower shell 1 is further provided with six insertion holes 12.

[0056] As Figures 3-4As shown, the test strip 2 includes three test strips, namely test strip 13, test strip 14 and test strip 15. The test strip 13 is fixed in the limiting groove 5, the test strip 14 is fixed in the limiting groove 6, and the test strip 15 is fixed in the limiting groove 7. Each test strip 2 is composed of a percolation membrane 16, an absorbent paper 17 and a fixed plate 18. The absorbent paper 17 is located at the lowermost layer, the percolation membrane 16 is located in the middle of the absorbent paper 17, and the fixed plate 18 is located on both sides of the percolation membrane 16 and is made of a fixing material. The lower surface of the fixed plate 18 is fixed with the absorbent paper, and the upper surface of the fixed plate 18 is provided with an adhesive tape. The adhesive tapes of the two fixed plates 18 can adhere to both sides of the upper surface of the percolation membrane 16 to fix the percolation membrane 16 in the middle of the absorbent paper. The percolation membrane 16 has three layers, including a first percolation membrane 20, a second percolation membrane 21 and a third percolation membrane 22. The first percolation membrane 20 of the test strip 13 is coated with anti-A monoclonal antibody containing red dye, the first percolation membrane 20 of the test strip 14 is coated with anti-B monoclonal antibody containing yellow dye, and the first percolation membrane 20 of the test strip 15 is coated with anti-D monoclonal antibody containing green dye. Red, yellow and green are the most easily distinguishable typical colors with the naked eye, so the dyes of these three colors are selected as the identifiers of different antibodies. In addition, coating the first percolation membrane 20 coated with different monoclonal antibodies with different colors can avoid the possibility of confusion in the manufacturing process of the test strip. The first percolation membrane 20 as a solid carrier is coated with antibodies. When the sample contacts the first percolation membrane 20 at the uppermost layer, if the red blood cell surface antigen in the sample reacts with the antibodies in the solid carrier, the diameter of the red blood cell becomes larger, and after the washing liquid is washed, the red blood cell does not sink and remains on the first percolation membrane 20, so the first percolation membrane 20 shows a blood red color, which is a positive reaction. On the contrary, if the red blood cell does not react, after the washing liquid is washed, the red blood cell sinks along the order from top to bottom through the first percolation membrane 20, the second percolation membrane 21 and the third percolation membrane 22, so the first percolation membrane 20 shows no color, which is a negative reaction. The sample to be tested sinks through the three layers of percolation membranes and is finally absorbed by the absorbent paper 17 at the bottom layer. The liquid will gradually penetrate to both sides of the absorbent paper 17 and be shunted and absorbed by the absorbent paper 17.

[0057] As Figure 1 , Figures 5-6As shown, the upper shell 3 is provided with three sample application holes 23, namely sample application holes 24, 25 and 26. Sample application hole 24 corresponds to the test strip 13 for detecting blood type A, sample application hole 25 corresponds to the test strip 14 for detecting blood type B, and sample application hole 26 corresponds to the test strip 15 for detecting Rh blood type. Elliptical recesses 27 are provided around each of the sample application holes 24, 25 and 26, with the three sample application holes located in the center of the elliptical recesses 27. The elliptical recesses 27 are used to temporarily store the added test samples and washing solution, providing a buffer space for capillary action, allowing the test samples to react more fully with the test strips and allowing the washing solution to rinse more thoroughly. Two second protrusions 28 are provided on the back of the elliptical recesses 27. These two second protrusions 28 can abut against the permeate membrane 16, strengthening the fixation of the permeate membrane 16 and preventing its displacement, ensuring that the three sample application holes are aligned with the permeate membrane 16. Six pins 29 are also provided on the inner edge of the upper housing 3, which correspond to the six insertion holes 12 of the lower housing 1.

[0058] The steps for using a human blood type filtration test card are as follows:

[0059] 1. Remove the sample from its storage conditions and allow it to equilibrate to room temperature;

[0060] 2. Allow the test card to reach room temperature and place it flat on the table;

[0061] 3. Use a dropper to draw up the sample to be tested. Add 10 μL of the whole blood sample to be tested to the sample window. After 1 minute, add 4 drops of diluent to the sample window.

[0062] 4. Wait 1 minute and observe the results at the sample loading window. Results observed after 30 minutes are invalid.

[0063] like Figures 7-10 As shown, the current housing has two limiting grooves to fix two test strips, and the upper housing has two sample dispensing holes.

[0064] While the present invention has been disclosed above, it is not limited thereto. Any person skilled in the art can make various modifications and alterations without departing from the spirit and scope of the present invention; therefore, the scope of protection of the present invention should be determined by the scope defined in the claims.

Claims

1. A blood group filtration test card, characterized in that, The application relates to a test strip box, which comprises an upper shell, a lower shell and test strips; the upper shell is provided with a sample adding hole, the lower shell is provided with a limiting groove, and a first protrusion is arranged in the limiting groove; the protrusion can not only lift the test strips to avoid contact between the test strips and the lower shell, but also can adjust the close degree of the test strips and the sample adding hole.

2. The blood group filtration test card according to claim 1, wherein The upper shell is provided with an elliptical recess, and the sample adding hole is located in the middle of the elliptical recess.

3. The blood group hemofiltration test card according to claim 2, wherein The back of the elliptical recess is provided with a second protrusion.

4. The blood group hemofiltration test card according to claim 3, wherein Limiting plates are arranged at both ends and both sides of the limiting groove.

5. The blood group hemofiltration test card according to claim 4, wherein The limiting plates arranged at both sides of the limiting groove comprise transverse limiting plates and longitudinal limiting plates.

6. The blood group hemofiltration test card according to claim 5, wherein The test strips comprise a percolation membrane, an absorbent paper and a fixing plate; the percolation membrane and the fixing plate are arranged on the absorbent paper, the percolation membrane is located in the middle of the absorbent paper, and the fixing plate is located at both sides of the percolation membrane.

7. The blood group hemofiltration test card according to claim 6, wherein An adhesive tape is arranged on the upper surface of the fixing plate, and one end of the fixing plate close to the percolation membrane is fixed with the percolation membrane through the adhesive tape.

8. The blood group hemofiltration test card according to claim 7, wherein The percolation membrane comprises a first percolation membrane, a second percolation membrane and a third percolation membrane, and the first percolation membrane is coated with an antibody.

9. The blood group hemofiltration test card according to claim 8, wherein The percolation membrane is stacked in the order of the first percolation membrane, the second percolation membrane and the third percolation membrane from top to bottom.

10. The blood group hemofiltration test card according to claim 9, wherein The number of the test strips comprises one or more, the number of the limiting grooves comprises one or more, the number of the sample adding holes comprises one or more, the lower shell is further provided with a jack, and the upper shell is further provided with a jack pin.

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