High-throughput coupling sample screening platform

Abstract

The utility model discloses a high-flux coupling small sample screening platform which comprises a 96-pore plate for providing antibodies and filler fixing matrixes, a membrane for intercepting filler is arranged on the pore plate, the pore plate is arranged on a base, a fixing frame for fixing the pore plate is arranged on the base, the base is connected with a hose, the other end of the hose is connected with a vacuum pump, and the vacuum pump is connected with a vacuum pump. The vacuum pump functions in the platform to remove filler on the membrane and antibodies bound to the filler. The problems that existing equipment is insufficient and new drug screening is time-consuming are solved, and the screening efficiency is greatly improved.

Description

Technical Field

[0001] This utility model relates to the field of biomedical devices, specifically to a high-throughput conjugate small sample screening device in the drug conjugate new drug development stage. Background Technology

[0002] The pharmaceutical field has no shortage of instruments and equipment for industrial production, as well as for analysis and testing. However, in the early stages of new drug development, the lack of usable equipment significantly reduces the efficiency of the experimental phase. For example, the recent development of antibody-drug conjugates (ADCs) has brought new breakthroughs to the pharmaceutical industry, leading to a concentration of resources in the new drug development stage. However, the availability of equipment cannot keep up with the pace of research, thus extending the development time. Antibody screening is a crucial and time-consuming process in the development of ADCs. Therefore, a high-throughput device capable of screening small batches during the development phase is needed. Utility Model Content

[0003] To address the above problems, this utility model discloses a high-throughput antibody-drug conjugation screening platform. This platform uses 96-well plates to screen small-batch antibody-drug conjugation experimental conditions, solving the problems of insufficient existing equipment and time-consuming new drug screening, and greatly improving screening efficiency.

[0004] The technical solution disclosed in this utility model is as follows:

[0005] A high-throughput conjugation screening platform includes a 96-well plate providing an antibody and a packing material immobilization matrix, and a membrane for intercepting the packing material is disposed on the plate.

[0006] The perforated plate is placed on a base and a fixing frame is used to fix the perforated plate. The base is connected to a hose, and the other end of the hose is connected to a vacuum pump. The vacuum pump plays a role in removing filler and antibodies bound to filler on the membrane in the platform.

[0007] Furthermore, the bottom of the base is closed, and a pipe hole is provided at the bottom for connecting a flexible hose.

[0008] Furthermore, the base is placed in a base groove and a vacuum is drawn.

[0009] Furthermore, the lower part of the base tank is provided with a drain hole and a hose connection port.

[0010] Compared with existing technologies, the high-throughput conjugate small-sample screening platform solves the problem that existing technologies do not have a suitable platform for conditional screening of conjugate drugs, thus improving the efficiency of antibody-drug conjugate development. Attached Figure Description

[0011] Figure 1 A schematic diagram of the structure of the base and perforated plate of this utility model;

[0012] Figure 2Structural diagram of the fixing frame, perforated plate, and base of this utility model;

[0013] Figure 3 Schematic diagram of the vacuum pump and base tank structure of this utility model;

[0014] In the figure, 1 is the orifice plate, 2 is the base, 3 is the fixing frame, 4 is the hose, 5 is the vacuum pump, 6 is the pipe hole, 7 is the base tank, 8 is the drain hole, and 9 is the hose connection port. Specific Implementation

[0015] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0016] A high-throughput conjugation small sample screening platform includes a 96-well plate 1 providing an antibody and a filler immobilization matrix. The plate 1 has a membrane for intercepting the filler. The plate is placed on a base 2 and a fixing frame 3 for fixing the plate. The base 2 is connected to a flexible tube 4, and the other end of the flexible tube 4 is connected to a vacuum pump 5. The vacuum pump 5 removes the filler and the antibody bound to the filler from the membrane in the platform.

[0017] The bottom of the base 2 is closed, and a pipe hole 6 is provided at the bottom for connecting the flexible hose 4.

[0018] The base 2 is placed in the base groove 7 and a vacuum is drawn.

[0019] The lower part of the base tank is provided with a drain hole 8 and a hose connection port 9.

[0020] Working principle: The obtained antibody and filler are placed in a 96-well plate, different coupling conditions are selected, eluent is added for elution, and a vacuum pump is used to evacuate the plate to obtain the coupled product.

[0021] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present invention.

Claims

1. A high-throughput coupling small-sample screening platform, characterized in that: The plate includes a 96-well plate (1) that provides antibodies and filler immobilization matrix. The plate (1) is provided with a membrane that intercepts filler. The plate (1) is placed on a base (2) and a fixing frame (3) is used to fix the plate. The base (2) is connected to a hose (4), and the other end of the hose is connected to a vacuum pump (5). The vacuum pump (5) removes filler and filler-bound antibodies from the membrane in the platform.

2. The high-throughput coupled small-sample screening platform according to claim 1, characterized in that: The bottom of the base (2) is closed, and a pipe hole (6) is provided at the bottom for connecting the hose (4).

3. The high-throughput coupled small-sample screening platform according to claim 2, characterized in that: The base (2) is placed in the base groove (7) for vacuuming.

4. The high-throughput coupled small-sample screening platform according to claim 3, characterized in that: The lower part of the base tank is provided with a drain hole (8) and a hose connection port (9).

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