Detection device based on absorption spectrometry

Abstract

The utility model relates to a detection device based on absorption spectroscopy, which comprises a bracket, a detection cavity and a detection probe, the micro-fluidic chip is arranged on the support, the micro-fluidic chip is provided with a plurality of micro-fluidic channels, detection areas are arranged in the micro-fluidic channels in an expanded mode, and at least the area, where the detection areas are located, of the micro-fluidic chip is located in the detection cavity; the injection pump is detachably connected to the micro-fluidic chip and is communicated with each micro-fluidic channel; the light detection assembly comprises a light emitter and a light receiver which are oppositely arranged on the two sides of the micro-fluidic chip, the light emitter comprises light emitting tubes which correspond to the detection areas in the micro-fluidic channels and are embedded in the wall of the detection cavity, and the light receiver comprises light receiving tubes which correspond to the detection areas in the micro-fluidic channels and are embedded in the wall of the detection cavity; and the control module is in electric signal connection with the micro-fluidic chip, the optical detection assembly and the injection pump. The detection device based on the absorption spectrometry is small and portable in structure, can accurately detect trace liquid, and can greatly shorten the detection time.

Description

Technical Field

[0001] This utility model relates to the field of absorption spectroscopy technology, specifically to a detection device based on absorption spectroscopy. Background Technology

[0002] Enzyme-linked immunosorbent assays (ELISA) are characterized by their speed, sensitivity, and standardization, playing a vital role in the early diagnosis and personalized treatment of diseases. ELISA based on immunomagnetic beads typically relies on multiple large analytical instruments. While this method offers high sensitivity and reduces reagent consumption and costs to some extent, the experimental procedures become more complex. It requires magnetic separation of the magnetic bead suspension before detection. If a small number of magnetic beads remain in the separated liquid, the resulting scattering will increase the detection error.

[0003] Developing a highly automated and portable enzyme-linked immunosorbent assay (ELISA) system based on immunomagnetic beads can not only reduce the cost of materials and personnel, but also enable automated and high-precision point-of-care testing (POCT). POCT can quickly and accurately diagnose diseases without the need for professional personnel and laboratory equipment. Its development will promote the advancement of global medical standards and is of great significance.

[0004] Currently, most portable POCT (Point-of-Care Testing) devices are used in conjunction with smartphones. However, these methods also have the following problems: 1. In dark environments, due to the smartphone's automatic exposure and color restoration algorithms, the acquired sample images may fail to reflect the true colors, leading to serious deviations in the test results, and even false negatives; 2. When testing low-concentration samples, smartphones are not sensitive to changes in sample concentration, exhibiting low sensitivity. Therefore, how to achieve portable, automated, easy-to-operate, and highly accurate rapid disease detection is a key issue that urgently needs to be addressed in the development of POCT testing platforms. Utility Model Content

[0005] The technical problem to be solved by this utility model is to provide a detection device based on absorption spectroscopy, which is small and portable, can accurately detect trace amounts of liquid, and can greatly shorten the detection time.

[0006] The technical solution adopted by this utility model to solve the above-mentioned technical problems is: a detection device based on absorption spectroscopy, characterized in that: it includes...

[0007] The support has a detection chamber inside;

[0008] A microfluidic chip is mounted on a support. The microfluidic chip has multiple microfluidic channels, and a detection area is enlarged in each microfluidic channel. At least the area where the detection area is located is located within the detection cavity of the microfluidic chip.

[0009] The syringe pump is detachably connected to the microfluidic chip and communicates with each microfluidic channel.

[0010] The optical detection component includes an optical emitter and an optical receiver disposed opposite to each other on both sides of a microfluidic chip. The optical emitter includes an optical emitting tube embedded in the detection cavity wall corresponding to the detection area in each microfluidic channel, and the optical receiver includes an optical receiving tube embedded in the detection cavity wall corresponding to the detection area in the microfluidic channel.

[0011] The control module is connected to the microfluidic chip, the photodetector, and the syringe pump via electrical signals.

[0012] For ease of assembly, the light transmitter further includes a light emitting circuit board, and the light receiver further includes a light receiving circuit board;

[0013] The top plate of the detection cavity has a first mounting hole corresponding to each light emitting tube. The light emitting tube is embedded in the first mounting hole. The light emitting circuit board is covered on the upper surface of the top plate of the detection cavity and is electrically connected to each light emitting tube.

[0014] The bottom plate of the detection cavity has a second mounting hole corresponding to each optical receiver tube. The optical receiver tube is embedded in the second mounting hole. The optical receiver circuit board is covered and disposed on the lower surface of the bottom plate of the detection cavity and is electrically connected to each optical receiver tube.

[0015] Preferably, the microfluidic channel includes, in sequence along the flow direction of the fluid, an injection section, an outwardly expanding reaction zone, a continuously curved flow control section, and the detection zone.

[0016] To ensure the accuracy of the microfluidic chip's position after assembly into the detection cavity and to improve the ease of assembly, the support includes a first frame and a second frame disposed on the right side of the first frame, with the microfluidic chip horizontally disposed on the second frame.

[0017] The first frame is provided with the detection cavity, and the first frame has a through hole for inserting the microfluidic chip into the detection cavity.

[0018] Optionally, the right sidewall of the detection cavity is provided with a through hole that matches the height of the second frame, the thickness and width of the microfluidic chip; or

[0019] The detection chamber has an opening on the right side, and a cover plate is provided on the opening. The cover plate has a through hole that matches the height of the second frame and the thickness and width of the microfluidic chip.

[0020] With a simple structure, the cover plate is provided with legs on the front and rear sides of its lower edge, and the cover plate is supported on the first frame by the legs;

[0021] The cover plate has a notch formed at the bottom based on the support legs, and the notch forms the through hole.

[0022] To ensure the stability of the cover plate, the second frame is provided with a limiting groove corresponding to the support position of the support leg, and the support leg is located in the limiting groove.

[0023] To ensure the accuracy of the microfluidic chip assembly position, the second frame is equipped with a positioning component for positioning the microfluidic chip.

[0024] Preferably, connecting components are matched on the mating surfaces of the first frame and the second frame.

[0025] Simply put, the connecting assembly includes a matching screw post and screw hole.

[0026] Compared with existing technologies, the advantages of this invention are as follows: The detection device based on absorption spectroscopy in this invention incorporates a microfluidic chip and an optical detection component on a support, enabling the detection of micro-flow fluid media using spectroscopy to obtain fluid medium parameter data. A single detection time can reach the microsecond level, significantly shortening the detection time. The microfluidic chip allows for precise micro-fluid control down to tens of micro-levels, thereby minimizing the required fluid medium volume while ensuring the accuracy of the optical detection component, effectively solving the problem that absorption spectroscopy cannot accurately detect trace amounts of liquids. Attached Figure Description

[0027] Figure 1 This is a perspective view of the detection device based on absorption spectroscopy in an embodiment of this utility model.

[0028] Figure 2 for Figure 1 Another perspective view.

[0029] Figure 3 This is an exploded perspective view of the detection device based on absorption spectroscopy in an embodiment of this utility model. Detailed Implementation

[0030] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments.

[0031] like Figures 1 to 3 As shown, the detection device based on absorption spectroscopy in this embodiment includes a support 1, a microfluidic chip 2, an injection pump, a photodetector 3, and a control module.

[0032] The support 1 has a detection cavity 11 inside, which is used to realize the spectral detection of the fluid medium.

[0033] The microfluidic chip 2 is mounted on the support 1. The microfluidic chip 2 can be any existing microfluidic chip 2 based on the fluid medium to be detected. Since this detection device based on absorption spectroscopy achieves detection using absorption spectroscopy, it needs to be made of a material with excellent light transmittance, such as polydimethylsiloxane, polymethyl methacrylate, or glass. The microfluidic chip 2 typically includes an upper chip and a lower chip; the two chips, when aligned, form a microfluidic channel 21. In this embodiment, the microfluidic chip 2 has multiple microfluidic channels 21, thus enabling simultaneous detection of parameters for multiple fluid media or multiple sets of parameter data for the same fluid medium.

[0034] The microfluidic channel 21, along the fluid flow direction, sequentially includes an injection section 211, an outwardly expanding reaction zone 212, a continuously curved flow control section 213, and an enlarged detection zone 214. The injection section 211 typically includes multiple branch channels, facilitating the injection of various fluid media to be reacted into the microfluidic channel 21. The reaction zone 212 promotes reactions between the incoming fluid media, while the flow control section 213 effectively controls the flow rate, thereby controlling the amount of fluid entering the detection zone 214. In this embodiment, the detection zone 214 is circular, with a diameter of 6 mm and a depth of 1.8 mm, resulting in a total volume of 50 μL. Based on the structure of the microfluidic channel 21, precise detection of micro-flow rates of fluid media is achieved.

[0035] At least the detection area 214 in the microfluidic chip 2 is located in the detection cavity 11, and the corresponding optical detection component 3 is also set in the area of ​​the detection cavity 11, thereby working with the microfluidic chip 2 to achieve spectral detection of micro-flow fluid media.

[0036] The injection pump is detachably connected to the microfluidic chip 2 and communicates with each microfluidic channel 21. After the injection pump is turned on, the fluid medium enters the reaction zone 212 through the injection section. After fully reacting in the reaction zone 212, it passes through the flow control section 213. The flow control section 213 can effectively reduce the flow rate, achieving precise control of the microfluidic medium and extending the reaction time of the fluid medium, resulting in a more complete reaction. In this embodiment, after the fluid medium flows through the flow control section 213, the injection pump is immediately turned off, and the fluid medium flows into the detection zone 214 based on inertia, filling the detection zone 214.

[0037] The optical detection component 3 includes a light emitter 31 and a light receiver 32 disposed opposite each other on both sides of the microfluidic chip 2. The light emitter 31 includes a light emitting tube 311 embedded in the wall of the detection cavity 11 corresponding to the detection area 214 in each microfluidic channel 21. The light receiver 32 includes a light receiving tube 321 embedded in the wall of the detection cavity 11 corresponding to the detection area 214 in the microfluidic channel 21. During operation, the operation of each light emitting tube 311 and light receiving tube 321 is controlled. The resistance of the light receiving tube 321 is inversely proportional to the light intensity of the fluid medium being measured; the weaker the light intensity of the fluid medium, the greater the resistance of the light receiving tube 321. By collecting the resistance data of the light receiving tube 321, the absorbance data of the fluid medium can be calculated. During the research and development phase, a precise detection instrument and this absorption spectroscopy-based detection device can be used to simultaneously detect parameters of the same fluid medium, thereby obtaining the corresponding detection data conversion formula. The conversion formula is pre-stored in the control module, and then converted into the required parameters based on the detected spectral data.

[0038] The control module is electrically connected to the microfluidic chip 2, the photodetector 3, and the syringe pump, thereby controlling the operation of these electrical devices and acquiring the feedback signals from them. The control module can be externally mounted as needed, or it can be directly mounted on the bracket 1. When mounted on the bracket 1, it can be mounted on the first frame 101 described below.

[0039] To facilitate the assembly of the light transmitter 31 and the light receiver 32, the light transmitter 31 further includes a light emitting circuit board 312, and the light receiver 32 further includes a light receiving circuit board 322. A first mounting hole 111 is provided on the top plate of the detection cavity 11 for each light emitting tube 311, and the light emitting tube 311 is embedded in the first mounting hole 111. The light emitting circuit board 312 covers the upper surface of the top plate of the detection cavity 11 and is electrically connected to each light emitting tube 311. A second mounting hole 112 is provided on the bottom plate of the detection cavity 11 for each light receiving tube 321, and the light receiving tube 321 is embedded in the second mounting hole 112. The light receiving circuit board 322 covers the lower surface of the bottom plate of the detection cavity 11 and is electrically connected to each light receiving tube 321.

[0040] In this embodiment, the light emitting tube 311 and the light receiving tube 321 are arranged opposite each other in the vertical direction. Therefore, the first mounting hole 111 is formed on the upper cavity wall of the detection cavity 11, and the second mounting hole 112 is formed on the lower cavity wall of the detection cavity 11. Accordingly, the light emitting circuit board 312 is disposed above the upper cavity wall of the detection cavity 11, and the light receiving circuit board 322 is disposed below the lower cavity wall of the detection cavity 11. In this way, the light emitting circuit board 312 can cover each of the first mounting holes 111 from above, and the light receiving circuit board 322 can cover each of the second mounting holes 112 from below, preventing light leakage during operation and affecting the detection results.

[0041] To ensure the accuracy of the microfluidic chip 2's position after assembly into the detection cavity 11, and to improve the ease of assembly, while also facilitating the modularization of the detection device based on absorption spectroscopy, thereby achieving a compact and portable structure, the support 1 in this embodiment includes a first frame 101 and a second frame 102 disposed to the right of the first frame 101. The detection cavity 11 is disposed within the first frame 101, and the bottom surface of the detection cavity 11 is flush with the upper surface of the second frame 102. The microfluidic chip 2 is horizontally disposed on the second frame 102, such that the detection areas 214 of each microfluidic channel 21 on the microfluidic chip 2 are suspended outside the second frame 102. After the first frame 101 and the second frame 102 are aligned, the detection areas 214 of each microfluidic channel 21 on the microfluidic chip 2 can extend into the detection cavity 11 of the first frame 101, thereby enabling the detection of parameters of the fluid medium.

[0042] In this embodiment, the first frame 101 has a through hole for inserting the microfluidic chip 2 into the detection cavity 11. The through hole structure is specifically determined according to the needs. For example, the detection cavity 11 on the first frame 101 can be directly set as a relatively closed cavity structure, and then the through hole matching the height of the second frame 102 and the thickness and width of the microfluidic chip 2 can be directly opened on the right side wall of the detection cavity 11. Alternatively, the right side of the detection cavity 11 can be open, and a cover plate 103 can be provided on the opening. The cover plate 103 has a through hole matching the height of the second frame 102 and the thickness and width of the microfluidic chip 2. In this embodiment, the cover plate 103 structure is adopted, and the through hole is provided on the cover plate 103. This structure has a more open space, which facilitates the assembly operation. During operation, the setting area of ​​the detection area 214 on the microfluidic chip 2 is first inserted into the detection cavity 11, and after confirming the accuracy of its position, the cover plate 103 is then closed.

[0043] The cover plate 103 is attached to the opening on the right side of the detection chamber 11 and covers the detection chamber 11. Since the cover plate 103 is vertically arranged, it achieves coverage. In order to ensure the stability of the cover plate 103 installation, support feet 1031 are respectively provided on the front and rear sides of the lower edge of the cover plate 103. The cover plate 103 is supported on the first frame 101 by the support feet 1031. The second frame 102 is provided with a limiting groove 1021 corresponding to the support position of the support feet 1031. The support feet 1031 are located in the limiting groove 1021, which greatly improves the installation stability of the cover plate 103.

[0044] The cover plate 103 has a notch 1032 formed at the bottom based on the support 1031. The notch 1032 and the opening of the detection cavity 11 are staggered by overlapping to form a through hole. This structure makes it easier to install the microfluidic chip 2. The through hole is formed when the cover plate 103 is installed. The support 1031 and the lower edge of the cover plate 103 are attached to the microfluidic chip 2, which can form a better limit for the microfluidic chip 2.

[0045] To ensure the accuracy of the assembly position of the microfluidic chip 2, the second frame 102 is provided with positioning members 1022 for positioning the microfluidic chip 2. In this embodiment, the positioning is achieved by positioning ribs on several sides other than the mating surface. When the microfluidic chip 2 is placed on the second frame 102 based on the positioning ribs, it is directly in the accurate position. At this time, when the microfluidic chip 2 is inserted into the detection cavity 11 of the first frame 101 based on the mating of the first frame 101 and the second frame 102, the detection area 214 on the microfluidic chip 2 can be precisely aligned with the light generator tube and the light receiver tube 321.

[0046] To prevent the microfluidic chip 2 from shifting during the detection process, a connecting component 4 is provided on the mating surfaces of the first frame 101 and the second frame 102. The connecting component 4 includes matching screw posts and screw holes. After the first frame 101 and the second frame 102 are connected based on the screw posts and screw holes, their positions are stable, and the relative positions of the microfluidic chip 2 and the photodetector 3 are also stable.

[0047] The detection device based on absorption spectroscopy in this invention incorporates a microfluidic chip 2 and an optical detection component 3 mounted on a support 1. This enables the detection of minute flow rates of fluid media using spectroscopy, thereby acquiring parameter data of the fluid medium. A single detection can be performed in microseconds, significantly reducing the overall detection time. The microfluidic chip 2 allows for precise micro-fluid control down to tens of micro-levels, thus minimizing the required fluid medium volume while ensuring the accuracy of the optical detection component 3. This effectively solves the problem of absorption spectroscopy's inability to accurately detect minute amounts of liquid.

[0048] In the specification and claims of this utility model, terms indicating direction, such as "front," "rear," "upper," "lower," "left," "right," "side," "top," and "bottom," are used to describe various exemplary structural parts and elements of the invention. However, the use of these terms is merely for illustrative purposes and is based on the exemplary orientations shown in the accompanying drawings. Since the embodiments disclosed in this invention can be arranged in different orientations, these terms indicating direction are for illustrative purposes only and should not be considered as limitations. For example, "upper" and "lower" are not necessarily limited to directions opposite to or consistent with the direction of gravity.

Claims

1. A detection device based on absorption spectroscopy, characterized in that: include The bracket (1) has a detection cavity (11) inside; A microfluidic chip (2) is mounted on a support (1). The microfluidic chip (2) has multiple microfluidic channels (21). A detection area (214) is enlarged in the microfluidic channel (21). At least the area where the detection area (214) is located is located in the detection cavity (11) of the microfluidic chip (2). The injection pump is detachably connected to the microfluidic chip (2) and communicates with each microfluidic channel (21); The optical detection component (3) includes an optical emitter (31) and an optical receiver (32) disposed opposite each other on both sides of the microfluidic chip (2). The optical emitter (31) includes an optical emitting tube (311) embedded in the wall of the detection cavity (11) corresponding to the detection area (214) in each microfluidic channel (21). The optical receiver (32) includes an optical receiving tube (321) embedded in the wall of the detection cavity (11) corresponding to the detection area (214) in the microfluidic channel (21). The control module is connected to the microfluidic chip (2), the photodetector (3), and the injection pump via electrical signals.

2. The detection device based on absorption spectroscopy according to claim 1, characterized in that: The light transmitter (31) further includes a light emitting circuit board (312), and the light receiver (32) further includes a light receiving circuit board (322); The top plate of the detection cavity (11) is provided with a first mounting hole (111) corresponding to each light emitting tube (311), the light emitting tube (311) is embedded in the first mounting hole (111), and the light emitting circuit board (312) is covered and disposed on the upper surface of the top plate of the detection cavity (11) and is electrically connected to each light emitting tube (311). The bottom plate of the detection cavity (11) has a second mounting hole (112) corresponding to each optical receiver tube (321). The optical receiver tube (321) is embedded in the second mounting hole (112). The optical receiver circuit board (322) is covered and disposed on the lower surface of the bottom plate of the detection cavity (11) and is electrically connected to each optical receiver tube (321).

3. The detection device based on absorption spectroscopy according to claim 1, characterized in that: The microfluidic channel (21) includes, in sequence along the flow direction of the fluid, an injection section (211), an outwardly expanded reaction zone (212), a continuously curved flow control section (213), and a detection zone (214).

4. The detection device based on absorption spectroscopy according to any one of claims 1 to 3, characterized in that: The support (1) includes a first frame (101) and a second frame (102) disposed on the right side of the first frame (101), and the microfluidic chip (2) is horizontally disposed on the second frame (102); The first frame (101) is provided with the detection cavity (11), and the first frame (101) has a through hole for inserting the microfluidic chip (2) into the detection cavity (11).

5. The detection device based on absorption spectroscopy according to claim 4, characterized in that: The detection cavity (11) has a through hole on its right side wall that matches the height of the second frame (102) and the thickness and width of the microfluidic chip (2); or The detection chamber (11) has an opening on the right side, and a cover plate (103) is provided on the opening. The cover plate (103) has a through hole that matches the height of the second frame (102), the thickness and width of the microfluidic chip (2).

6. The detection device based on absorption spectroscopy according to claim 5, characterized in that: The cover plate (103) is provided with support feet (1031) on the front and rear sides of the lower edge, and the cover plate (103) is supported on the first frame (101) by the support feet (1031); The cover plate (103) has a notch (1032) formed at the bottom based on the support (1031), and the notch (1032) constitutes the through hole.

7. The detection device based on absorption spectroscopy according to claim 6, characterized in that: The second frame (102) is provided with a limiting groove (1021) corresponding to the support position of the support leg (1031), and the support leg (1031) is located in the limiting groove (1021).

8. The detection device based on absorption spectroscopy according to claim 4, characterized in that: The second frame (102) is provided with a positioning element (1022) for positioning the microfluidic chip (2).

9. The detection device based on absorption spectroscopy according to claim 4, characterized in that: A connecting component (4) is provided on the mating surfaces of the first frame (101) and the second frame (102).

10. The detection device based on absorption spectroscopy according to claim 9, characterized in that: The connecting component (4) includes a matching screw post and screw hole.

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