Protein concentration device

Abstract

The utility model discloses a protein concentration device which comprises an ultrafiltration membrane assembly, the output end and the input end of the ultrafiltration membrane assembly are respectively provided with a connecting pipe, the other ends of the two connecting pipes are respectively provided with a pressure monitor, the pressure monitors monitor the pressure difference of the two sides of an ultrafiltration membrane in real time, and when the pressure difference is close to the upper limit of a set threshold value, the ultrafiltration membrane assembly is started. The control system reduces the rotating speed of the peristaltic pump, reduces the flow rate of the solution and reduces the pressure difference, so that the ultrafiltration membrane assembly is more stable in filtration, and a conveying pipe is mounted on one side of the pressure monitor at the upper end; the concentration mode and degree can be flexibly selected according to requirements, the protein concentration efficiency is greatly improved, compared with a traditional centrifugal concentration method and a dialysis concentration method, the concentration time can be shortened, meanwhile, the heat preservation cover, the Peltier and the heat dissipation ring pieces are matched for heat preservation of the exterior of the ultrafiltration membrane assembly, and the heat preservation effect is good. The denaturation and inactivation risk of the protein in the concentration process is reduced, it is ensured that the concentrated protein still has high biological activity, and the protein concentration quality is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of biological medicine protein, specifically is a protein concentration device. BACKGROUND

[0002] Biological medicine protein has a crucial position in the field of modern medicine and life science. Protein is the main executor of life activities and participates in almost all physiological processes, such as metabolism, signal transduction, immune response, and cell structure maintenance. In the aspect of disease diagnosis, the markers of many diseases are specific proteins, and through detecting the existence, content or activity change of these proteins, early diagnosis and disease monitoring can be realized. For example, the detection of tumor marker proteins (such as carcinoembryonic antigen, alpha-fetoprotein, etc.) has important significance for early detection and treatment effect evaluation of cancer. In the field of disease treatment, biological medicine protein also plays an increasingly important role.

[0003] In the field of biological medicine, protein concentration is crucial, but traditional concentration methods such as centrifugal concentration, dialysis concentration and freeze-drying concentration have many limitations. Centrifugal concentration is prone to protein denaturation and inactivation, has low efficiency for large-scale processing and is difficult to control concentration accurately; dialysis concentration is time-consuming, easy to contaminate and difficult to control concentration; freeze-drying concentration requires expensive equipment, is complex to operate and the product is prone to caking.

[0004] Therefore, there is an urgent need for a new protein concentration device that can overcome these problems. UTILITY MODEL CONTENT

[0005] The utility model aims at providing a protein concentration device to solve the problems raised in the background art.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a protein concentration device, comprising an ultrafiltration membrane assembly, a connecting pipe is installed at the output end and the input end of the ultrafiltration membrane assembly, a pressure monitor is installed at the other end of the two connecting pipes, a conveying pipe is installed on one side of the upper end pressure monitor, a peristaltic pump is installed at the other end of the conveying pipe, a heat preservation cover is installed on the outer wall of the ultrafiltration membrane assembly, a Peltier device is installed inside the heat preservation cover, a heat dissipation ring piece matched with the outer side of the ultrafiltration membrane assembly is installed on one side of the Peltier device, a three-way valve is installed on one side of the lower end pressure monitor, a discharge pipe is installed at the lower end of the three-way valve, a vacuum tank is rotatably installed below the outer wall of the discharge pipe, a vacuumizing mechanism is arranged on one side of the vacuum tank, a vacuum concentration tank is arranged inside the vacuum tank and is connected with the discharge pipe, a driving mechanism and a discharging mechanism are arranged below the vacuum concentration tank to drive it to rotate.

[0007] Preferably, the inner wall of the vacuum concentration box is rotationally connected below the outer wall of the feeding pipe, and the inside of the ultrafiltration membrane assembly, the feeding pipe, the vacuum box and the vacuum concentration box are connected through.

[0008] Preferably, the vacuum mechanism comprises an evacuation pipe, the evacuation pipe is connected through the inside of the vacuum box and the vacuum concentration box, one side of the evacuation pipe is provided with a plurality of valves, and the other end of the evacuation pipe is provided with an air pump.

[0009] Preferably, the driving mechanism comprises a fixed seat, the fixed seat is installed on the vacuum box, a permanent magnet rotor is rotationally installed in the inside of the fixed seat, a connecting plate is installed on the top of the permanent magnet rotor, and the top of the connecting plate is installed on the vacuum concentration box.

[0010] Preferably, the driving mechanism further comprises a copper rotor which is magnetically coupled with the permanent magnet rotor, a rotating seat is installed at the bottom of the copper rotor, a driving motor is installed at the bottom of the rotating seat, and a machining table is installed on one side of the driving motor.

[0011] Preferably, the air pump is installed on the machining table, an installation table is installed on the top of the machining table, a supporting seat is installed on the top of the installation table, and one side of the ultrafiltration membrane assembly and the peristaltic pump is installed on the installation table.

[0012] Preferably, the discharging mechanism comprises a discharging valve, the upper end of the discharging valve is connected through the inside of the vacuum concentration box, the lower end of the discharging valve is provided with a discharging pipe, and the lower end of the discharging pipe penetrates through the copper rotor and extends to the outside.

[0013] Preferably, a mounting seat is installed below the inner wall of the vacuum box, and a heating pipe is installed on the top of the mounting seat.

[0014] Preferably, a control panel is installed on the outer wall of the heat preservation cover, a temperature monitor which is electrically connected with the control panel is installed on the outer wall of the conveying pipe, the temperature monitor is used for monitoring the temperature of the material conveyed by the peristaltic pump, the vacuum box is made of high-strength transparent acrylic material, and the vacuum concentration box is made of borosilicate glass material.

[0015] Compared with the prior art, the protein concentration device has the following beneficial effects:

[0016] 1. The ultrafiltration membrane assembly 1, the peristaltic pump 5, the vacuum concentration box 15 and the vacuum mechanism are combined, the concentration mode and degree can be flexibly selected according to requirements, the protein concentration efficiency is greatly improved, the concentration time can be shortened compared with the traditional centrifugal concentration method and dialysis concentration method, the heat preservation cover, the Peltier and the heat dissipation ring piece are matched to perform heat preservation on the outside of the ultrafiltration membrane assembly, the denaturation and inactivation risk of the protein in the concentration process is reduced, the protein after concentration still has high biological activity, and the protein concentration quality is improved.

[0017] 2、 The utility model discloses a mounting seat and the setting of heating pipe are set up, and the material is along the inner wall of vacuum concentration box and shakes when the vacuum concentration box is rotated in the inside of vacuum box through the drive mechanism control, and cooperate the heating pipe of installing on the mounting seat and heat the material in the inside of vacuum concentration box, make the temperature of protein solution increase, and the molecule movement intensifies, and further promote the evaporation of solvent and water, improve the concentration purity of protein. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 It is the main structure schematic diagram of the utility model;

[0019] Figure 2 It is the section structure schematic diagram of the utility model;

[0020] Figure 3 It is Figure 2 the enlarged view of A in

[0021] Figure 4 It is Figure 2 the enlarged view of B in

[0022] Figure 5 It is the partial section structure schematic diagram of the utility model.

[0023] In the drawing: 1, ultrafiltration membrane assembly;2, connecting pipe;3, pressure monitor;4, conveying pipe;5, peristaltic pump;6, three-way valve;7, heat preservation cover;8, peltier;9, heat dissipation ring piece;10, blanking pipe;11, vacuum box;12, fixed seat;13, permanent magnet rotor;14, connecting plate;15, vacuum concentration box;16, discharge valve;17, discharge pipe;18, copper rotor;19, rotating seat;20, drive motor;21, evacuation pipe;22, multi-way valve;23, air pump;24, mounting seat;25, heating pipe;26, support seat;27, mounting table;28, processing table;29, control panel;299, temperature monitor. DETAILED DESCRIPTION

[0024] The technical scheme in the embodiments of the utility model will be described clearly and completely in combination with the drawings in the embodiments of the utility model, and apparently, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by the person skilled in the art without creative labor belong to the range of protection of the utility model.

[0025] Embodiment 1: please refer to Figures 1-5The utility model provides a technical scheme: a protein concentration device, including ultrafiltration membrane subassembly 1, the output and input of ultrafiltration membrane subassembly 1 are all installed with connecting pipe 2, the other end of two connecting pipes 2 is all installed with pressure monitor 3, and pressure monitor 3 real -time monitoring pressure difference of both sides of ultrafiltration membrane, when pressure difference approaches set threshold upper limit, control system reduces peristaltic pump speed, reduces solution flow rate, reduces pressure difference, makes the more stable filtration of ultrafiltration membrane subassembly 1, and the side of upper end pressure monitor 3 is installed with delivery pipe 4, and the other end of delivery pipe 4 is installed with peristaltic pump 5, and the outer wall of ultrafiltration membrane subassembly 1 is installed with heat preservation cover 7, and the inside of heat preservation cover 7 is installed with peltier 8, and the side of peltier 8 is installed with the heat dissipation ring piece 9 matched with the outside of ultrafiltration membrane subassembly 1, and the side of lower end pressure monitor 3 is installed with three -way valve 6, and the lower end of three -way valve 6 is installed with downcomer 10, and the lower of downcomer 10 outer wall is rotatably installed with vacuum tank 11, and one side of vacuum tank 11 is equipped with vacuumizing mechanism, and the inside of vacuum tank 11 is equipped with the vacuum concentration tank 15 of through connection with downcomer 10, and the lower of vacuum concentration tank 15 is equipped with the drive mechanism and discharge mechanism of driving its rotation, and through the combination of ultrafiltration membrane subassembly 1 and peristaltic pump 5 and vacuum concentration tank 15 and vacuum mechanism, can flexibly select concentration mode and degree according to demand, greatly improve protein concentration efficiency, compared with traditional centrifugal concentration method and dialysis concentration method, concentration time can be shortened, and at the same time, cooperate heat preservation cover 7 and peltier 8 and heat dissipation ring piece 9 to carry out the heat preservation to the outside of ultrafiltration membrane subassembly 1, reduce the denaturation inactivation risk of protein in the concentration process, ensure that the protein after concentration still has higher biological activity, improve protein concentration quality, and the inner wall of vacuum concentration tank 15 is rotatably connected with the lower of downcomer 10 outer wall, and the inside of ultrafiltration membrane subassembly 1, downcomer 10, vacuum tank 11 and vacuum concentration tank 15 is through connection.

[0026] As Figure 2 The vacuum mechanism includes a vacuum pipe 21, which is connected to the inside of the vacuum tank 11 and the vacuum concentration tank 15, and a multi-valve 22 is installed on one side of the vacuum pipe 21. The other end of the vacuum pipe 21 is installed with a gas pump 23. The gas in the vacuum tank 11 is discharged through the vacuum pipe 21 and the multi-valve 22 by the gas pump 23 in the vacuum mechanism. When discharging, the pressure can be first released through the multi-valve 22, and then the discharge is more stable.

[0027] As Figure 2 and 3As shown, the driving mechanism includes a fixed seat 12 mounted on the vacuum box 11, the inside of the fixed seat 12 is rotatably mounted with a permanent magnet rotor 13, the top of the permanent magnet rotor 13 is mounted with a connecting plate 14, the top of the connecting plate 14 is mounted on the vacuum concentration box 15, the mechanism further includes a copper rotor 18 magnetically coupled with the permanent magnet rotor 13, the bottom of the copper rotor 18 is mounted with a rotating seat 19, the bottom of the rotating seat 19 is mounted with a driving motor 20, one side of the driving motor 20 is mounted with a machining table 28, a gas pump 23 is mounted on the machining table 28, the top of the machining table 28 is mounted with a mounting table 27, the top of the mounting table 27 is mounted with a support seat 26, one side of the ultrafiltration membrane assembly 1 and the peristaltic pump 5 is mounted on the mounting table 27, the driving motor 20 is started to drive the copper rotor 18 to rotate. The copper rotor 18 and the permanent magnet rotor 13 in the fixed seat 12 are magnetically coupled to transmit power to the permanent magnet rotor 13, and then drive the connecting plate 14 and the vacuum concentration box 15 to rotate, the rotation speed can be adjusted on the control panel 29, during the rotation, the protein solution forms a film along the inner wall of the vacuum concentration box 15 under the action of centrifugal force, which increases the evaporation area of the solvent, the heating pipe 25 on the mounting seat 24 heats the protein solution in the vacuum concentration box 15, the heating temperature is set on the control panel 29 according to the thermal stability of the protein and the characteristics of the solvent, heating intensifies the movement of protein solution molecules, further promotes solvent evaporation, and improves the concentration efficiency;

[0028] The discharging mechanism includes a discharge valve 16, the upper end of the discharge valve 16 is connected with the inside of the vacuum concentration box 15, the lower end of the discharge valve 16 is mounted with a discharge pipe 17, the lower end of the discharge pipe 17 penetrates the copper rotor 18 and extends to the outside, the discharge valve 16 controls the concentrated material and transports it through the discharge pipe 17.

[0029] As shown in Figure 1 and 2 As shown, the outer wall of the heat preservation cover 7 is mounted with a control panel 29, the outer wall of the conveying pipe 4 is mounted with a temperature monitor 299 electrically connected with the control panel 29, the temperature monitor 299 is used for monitoring the temperature of the material conveyed by the peristaltic pump 5, the temperature of the conveyed material is monitored in real time through the control panel 29 cooperating with the temperature monitor 299, which is convenient for the temperature of the material conveyed into the ultrafiltration membrane assembly 1 to be appropriate, and is convenient for the equipment to separate, the vacuum box 11 is made of high-strength transparent acrylic material, the vacuum box 11 is made of high-strength transparent acrylic material, which is convenient for observing the internal situation, the good sealing property ensures the stability of the vacuum environment, the vacuum concentration box 15 is made of borosilicate glass material, the vacuum concentration box 15 is made of borosilicate glass material, which has good chemical stability and high temperature resistance, and the surface is smooth and the discharge is convenient.

[0030] The working principle is that: in use, the protein solution to be concentrated is placed in a suitable container, connected with the peristaltic pump 5 through the conveying pipe 4, and the peristaltic pump 5 is started to deliver the protein solution to the ultrafiltration membrane assembly 1 at a precisely controlled flow rate. The flow rate of the peristaltic pump 5 can be set on the control panel 29. During the solution delivery process, the pressure monitor 3 on the input end connecting pipe 2 of the ultrafiltration membrane assembly 1 monitors the input pressure in real time. After the protein solution enters the ultrafiltration membrane assembly 1, under the pressure provided by the peristaltic pump 5, the solvent and small molecular impurities pass through the ultrafiltration membrane, while the large molecular substances such as proteins are intercepted. The molecular weight cut-off of the ultrafiltration membrane is selected according to the size of the target protein. If the concentration requirement is low, the concentrated material can be discharged through the lower three-way valve 6. At the same time, the heat or cold generated by the Peltier 8 is uniformly transmitted to the ultrafiltration membrane assembly 1 through the heat dissipation ring piece 9 in close contact with the outside of the ultrafiltration membrane assembly 1, and the heat preservation cover 7 plays a role in reducing heat loss or external heat interference, ensuring that the ultrafiltration process is carried out in a stable temperature environment, and improving the ultrafiltration efficiency.

[0031] When vacuum concentration is needed, the three-way valve 6 can be switched to the direction of the discharge pipe 10, so that the preliminarily concentrated protein solution enters the vacuum concentration tank 15 through the discharge pipe 10. The air pump 23 is started, and the vacuum tank 11 and the vacuum concentration tank 15 are evacuated through the evacuation pipe 21 and the multi-way valve 22. The multi-way valve 22 can control the adjustment of the vacuum degree, the on-off of different air paths and pressure relief, and at the same time, the driving motor 20 is started to drive the copper rotor 18 to rotate. The copper rotor 18 and the permanent magnet rotor 13 in the fixed seat 12 are coupled by magnetic force, and the power is transmitted to the permanent magnet rotor 13, and then the connecting plate 14 and the vacuum concentration tank 15 are rotated. Under the action of centrifugal force, the protein solution forms a thin film along the inner wall of the vacuum concentration tank 15, which increases the evaporation area of the solvent. Heating makes the protein solution molecules move more vigorously, further promoting solvent evaporation, improving protein concentration efficiency, and finally the concentrated material is discharged through the discharge valve 16 and the discharge pipe 17.

[0032] As shown in Figure 2 and 5 In some proteomics studies, higher concentration of protein samples is needed. After ultrafiltration concentration, vacuum concentration can be carried out. The lower wall of the vacuum tank 11 is provided with a mounting seat 24, and the top of the mounting seat 24 is provided with a heating pipe 25. The three-way valve 6 is switched to make the ultrafiltration concentrated protein solution enter the vacuum concentration tank 15. The temperature of the heating pipe 25 is set to a suitable temperature, and the temperature of the vacuum tank 11 and the vacuum concentration tank 15 is controlled in real time to a suitable evaporation temperature, so as to further accelerate the evaporation of the solvent and water in the protein in the vacuum concentration tank 15, and accelerate the concentration efficiency.

[0033] Although the embodiments of the present application have been shown and described, it is to be understood that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present application, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A protein concentration apparatus comprising an ultrafiltration membrane module (1), characterized in that: The output end and the input end of the ultrafiltration membrane assembly (1) are provided with connecting pipes (2), the other ends of the two connecting pipes (2) are provided with pressure monitors (3), one side of the upper end of the pressure monitor (3) is provided with a conveying pipe (4), the other end of the conveying pipe (4) is provided with a peristaltic pump (5), the outer wall of the ultrafiltration membrane assembly (1) is provided with a heat preservation cover (7), the inside of the heat preservation cover (7) is provided with a peltier (8), one side of the peltier (8) is provided with a heat dissipation ring piece (9) matched with the outside of the ultrafiltration membrane assembly (1), one side of the lower end of the pressure monitor (3) is provided with a three-way valve (6), the lower end of the three-way valve (6) is provided with a discharging pipe (10), the lower end of the outer wall of the discharging pipe (10) is rotatably provided with a vacuum box (11), one side of the vacuum box (11) is provided with a vacuumizing mechanism, the inside of the vacuum box (11) is provided with a vacuum concentration box (15) penetratingly connected with the discharging pipe (10), the lower end of the vacuum concentration box (15) is provided with a driving mechanism and a discharging mechanism driving it to rotate.

2. A protein concentration device according to claim 1, wherein: The inner wall of the vacuum concentration box (15) is rotatably connected with the lower end of the outer wall of the discharging pipe (10), and the inside of the ultrafiltration membrane assembly (1), the discharging pipe (10), the vacuum box (11) and the vacuum concentration box (15) are penetratingly connected.

3. The protein concentration device of claim 1, wherein: The vacuumizing mechanism comprises an evacuation pipe (21) penetratingly connected with the inside of the vacuum box (11) and the vacuum concentration box (15), one side of the evacuation pipe (21) is provided with a multi-way valve (22), and the other end of the evacuation pipe (21) is provided with an air pump (23).

4. A protein concentration device according to claim 3, wherein: The driving mechanism comprises a fixed seat (12) mounted on the vacuum box (11), a permanent magnet rotor (13) rotatably mounted in the inside of the fixed seat (12), and a connecting plate (14) mounted on the top of the permanent magnet rotor (13) and on the vacuum concentration box (15).

5. A protein concentration device according to claim 4, wherein: The driving mechanism further comprises a copper rotor (18) magnetically coupled with the permanent magnet rotor (13), a rotating seat (19) mounted on the bottom of the copper rotor (18), and a driving motor (20) mounted on the bottom of the rotating seat (19), and one side of the driving motor (20) is provided with a machining table (28).

6. A protein concentration device according to claim 5, wherein: The air pump (23) is mounted on the machining table (28), the top of the machining table (28) is provided with a mounting table (27), the top of the mounting table (27) is provided with a supporting seat (26), and one side of the ultrafiltration membrane assembly (1) and the peristaltic pump (5) is mounted on the mounting table (27).

7. The protein concentration device of claim 1, wherein: The discharging mechanism comprises a discharging valve (16) penetratingly connected with the inside of the vacuum concentration box (15), a discharging pipe (17) mounted on the lower end of the discharging valve (16), and the lower end of the discharging pipe (17) penetrates through the copper rotor (18) and extends to the outside.

8. The protein concentration device of claim 2, wherein: The lower end of the inner wall of the vacuum box (11) is provided with a mounting seat (24), and the top of the mounting seat (24) is provided with a heating pipe (25).

9. The protein concentration device of claim 1, wherein: The outer wall of the heat preservation cover (7) is provided with a control panel (29), the outer wall of the conveying pipe (4) is provided with a temperature monitor (299) electrically connected with the control panel (29), the temperature monitor (299) is used for monitoring the temperature of the material conveyed by the peristaltic pump (5), the vacuum box (11) is made of high-strength transparent acrylic material, and the vacuum concentration box (15) is made of borosilicate glass material.

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