Reagent model detection circuit for automatic dyeing equipment

Abstract

The utility model provides a reagent type detection circuit for automatic dyeing equipment, which comprises an output end of a power supply, a voltage regulation circuit and a dyeing reagent detection circuit which are in power supply connection, and an output end of the voltage regulation circuit is in power supply connection with a main control circuit of the automatic dyeing equipment. The output end of the automatic dyeing equipment main control circuit is in control connection with the input end of the dyeing reagent detection circuit, the output end of the dyeing reagent detection circuit is in feedback connection with the input end of the automatic dyeing equipment main control circuit, the automatic dyeing equipment main control circuit is further in communication connection with the touch display screen, and the touch display screen can input information in a touch mode. And a plurality of dyeing reagent model detection sensors are arranged in the dyeing reagent detection circuit. The device has the beneficial effects that the type of the dyeing reagent in the dyeing reagent temporary storage assembly can be automatically identified, so that the dyeing reagent temporary storage assembly is ensured to add and supplement the dyeing reagent with the correct type into the dyeing tank.

Description

Technical Field

[0001] This utility model relates to the field of circuit technology, specifically to a reagent type detection circuit for automatic staining equipment. Background Technology

[0002] In existing biological sample analysis techniques, commonly used analytical methods include microscopy, microarray analysis (e.g., protein and nucleic acid microarray analysis), and mass spectrometry. Preparing samples for these and other types of analysis typically involves contacting the biological sample with a series of processing liquids. Some of these processing liquids (e.g., staining reagents and counterstaining reagents) can add color and, conversely, alter the visual properties of invisible or difficult-to-see sample components (e.g., at least some types of cells and intracellular structures). Other processing liquids (e.g., deparaffin liquids) can be used to achieve other processing purposes. If multiple processing liquids are used to treat the sample, the application and subsequent removal of each liquid can be crucial for producing a sample suitable for analysis. In some cases, treating samples with multiple processing liquids involves manually applying the liquids to the microscope slides holding the samples. However, this manual sample preparation method tends to be labor-intensive and inaccurate, leading to the development of biological tissue section staining equipment.

[0003] Automated staining technology for biological tissue samples is a crucial and indispensable step in the entire automated staining, drying, and mounting process for biological tissue samples. To ensure that the processed biological tissue samples have an easily observable appearance and maintain consistency and efficiency in the processing, the staining step is an essential aspect that must be strictly controlled during biological tissue sample processing. The purpose of staining is to make different structures within the cellular tissue appear in different colors for easier observation. The classic hematoxylin and eosin staining method is the routine staining method for histological and pathological specimens, abbreviated as HE staining. After staining, the cell nuclei are stained purple-blue by hematoxylin, while most cytoplasm and non-cellular components are stained pink by eosin.

[0004] Existing biological tissue sample staining equipment can replace manual staining. These machines automatically process samples by immersing a rack holding a batch of microscope slides in an open bath containing processing liquid. However, during the immersion staining operation, existing biological tissue sample staining equipment requires frequent replenishment of various staining reagents as they are consumed over time. This manual replenishment is cumbersome, time-consuming, labor-intensive, and prone to errors, resulting in inconsistent production efficiency and product quality that fails to meet user needs. Utility Model Content

[0005] To address the problems in the prior art, this utility model provides a reagent type detection circuit for an automatic staining device. By setting up a power supply, voltage adjustment circuit, main control circuit of the automatic staining device, staining reagent detection circuit, and touch screen in a coordinated manner, the circuit can automatically identify the type of staining reagent in the staining reagent storage component, thereby ensuring that the correct type of staining reagent is added to the staining tank. This eliminates the need for manual operation, reduces errors, and significantly improves the production efficiency and product quality of microscope slides for biological tissue sample sections. It also solves the problem of time-consuming, labor-intensive, and error-prone manual addition of staining reagents during staining operations in existing biological tissue sample section staining equipment.

[0006] This utility model provides a reagent type detection circuit for an automatic staining device, including a power supply, a voltage adjustment circuit, an automatic staining device main control circuit, a staining reagent detection circuit, and a touch screen. The output terminal of the power supply is connected to the voltage adjustment circuit and the staining reagent detection circuit. The output terminal of the voltage adjustment circuit is connected to the automatic staining device main control circuit. The output terminal of the automatic staining device main control circuit is connected to the input terminal of the staining reagent detection circuit. The output terminal of the staining reagent detection circuit is also connected to the input terminal of the automatic staining device main control circuit. The automatic staining device main control circuit is also communicatively connected to the touch screen, which allows for touch input of information. The staining reagent detection circuit contains multiple staining reagent type detection sensors. The automatic staining device main control circuit can control the staining reagent detection circuit to detect the staining reagent type in the staining reagent temporary storage component of the automatic staining device and feed it back to the automatic staining device main control circuit for display on the touch screen.

[0007] This utility model is further improved in that the main control circuit of the automatic dyeing equipment is provided with a main control chip U5 and a fuse resistor FB13. The main control chip U5 has 140 pins. Pin 32 of the main control chip U5 is connected to pin 33 of the main control chip U5 and one end of the fuse resistor FB13. The other end of the fuse resistor FB13 is connected to the output terminal of the voltage adjustment circuit. Pins 110, 114, 115, 93, 98, 99, 90, and 91 of the main control chip U5 are connected to the dyeing reagent detection circuit. Pins 96, 97, and 113 of the main control chip U5 are connected to the touch screen display.

[0008] This utility model is further improved by including a voltage regulator chip U4, a diode D5, a resistor R16, a capacitor CT3, and a capacitor C28 in the voltage adjustment circuit. The voltage regulator chip U4 has four pins. The third pin of the voltage regulator chip U4 is connected to one end of the capacitor CT3, one end of the capacitor C28, and the output terminal of the power supply. The second pin of the voltage regulator chip U4 is connected to the fourth pin of the voltage regulator chip U4, one end of the resistor R16, and the other end of the fuse resistor FB13. The other end of the resistor R16 is connected to the positive terminal of the diode D5. The first pin of the voltage regulator chip U4, the other end of the capacitor CT3, the other end of the capacitor C28, and the negative terminal of the diode D5 are grounded.

[0009] In a further improvement to this invention, the voltage adjustment circuit is further provided with capacitors CT4, C29, C254, and C255. The second pin of the voltage regulator chip U4 is connected to one end of capacitor CT4, one end of capacitor C29, one end of capacitor C254, and one end of capacitor C255. The other ends of capacitors CT4, C29, C254, and C255 are grounded.

[0010] This utility model is further improved by including a staining reagent detection circuit comprising a staining reagent model detection sensor U18D, diode D10, staining reagent model detection sensor U18E, diode D11, staining reagent model detection sensor U18F, diode D12, staining reagent model detection sensor U18A, diode D13, staining reagent model detection sensor U18B, diode D14, staining reagent model detection sensor U18C, and diode D15. One end of the staining reagent model detection sensor U18D is connected to the negative terminal of diode D10 and pin 110 of the main control chip U5, while the positive terminal of diode D10 is connected to the output terminal of the power supply. One end of the staining reagent model detection sensor U18E is connected to the negative terminal of diode D11 and pin 114 of the main control chip U5, while the positive terminal of diode D11 is connected to the output terminal of the power supply. The output terminal of the power supply is connected. One end of the staining reagent model detection sensor U18F is connected to the negative terminal of the diode D12 and pin 115 of the main control chip U5. The positive terminal of the diode D12 is connected to the output terminal of the power supply. One end of the staining reagent model detection sensor U18A is connected to the negative terminal of the diode D13 and pin 93 of the main control chip U5. The positive terminal of the diode D13 is connected to the output terminal of the power supply. One end of the staining reagent model detection sensor U18B is connected to the negative terminal of the diode D14 and pin 98 of the main control chip U5. The positive terminal of the diode D14 is connected to the output terminal of the power supply. One end of the staining reagent model detection sensor U18C is connected to the negative terminal of the diode D15 and pin 99 of the main control chip U5. The positive terminal of the diode D15 is connected to the output terminal of the power supply.

[0011] This utility model is further improved by including a staining reagent type detection sensor U19D, a diode D16, a staining reagent type detection sensor U19E, and a diode D17 in the staining reagent type detection circuit. One end of the staining reagent type detection sensor U19D is connected to the negative terminal of the diode D16 and pin 90 of the main control chip U5, and the positive terminal of the diode D16 is connected to the output terminal of the power supply. One end of the staining reagent type detection sensor U19E is connected to the negative terminal of the diode D17 and pin 91 of the main control chip U5, and the positive terminal of the diode D17 is connected to the output terminal of the power supply.

[0012] This utility model is further improved in that the main control chip U5 is an STM32F407ZGT6, the voltage regulator chip U4 is an LM1085IS-3.3 / NOPB, and the staining reagent model detection sensors U18A, U18B, U18C, U18D, U18E, U18F, U19D, and U19E are all SN74LS14DR.

[0013] Compared with the prior art, the beneficial effects of this utility model are as follows: It provides a reagent type detection circuit for automatic staining equipment. By setting up a power supply, voltage adjustment circuit, main control circuit of automatic staining equipment, staining reagent detection circuit and touch screen in the reagent type detection circuit of automatic staining equipment, the main control circuit of automatic staining equipment can control the staining reagent detection circuit to detect the staining reagent type in the staining reagent temporary storage component of automatic staining equipment and feed it back to the main control circuit of automatic staining equipment and transmit it to the touch screen for display. It can realize the automatic identification of the staining reagent type in the staining reagent temporary storage component, thereby ensuring that the staining reagent temporary storage component adds the correct type of staining reagent to the staining tank. No manual operation is required, saving time and effort and reducing errors. It significantly improves the production efficiency and product quality of biological tissue sample section microscope slides, improves the user experience, and solves the problem that manual addition of staining reagent during staining operations in existing biological tissue sample section staining equipment is time-consuming, labor-intensive and prone to errors. Attached Figure Description

[0014] To more clearly illustrate the solutions in this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0015] Figure 1 This is a schematic diagram of a reagent type detection circuit for an automatic staining device according to the present invention.

[0016] Figure 2 This is a circuit diagram of the main control circuit of the automatic dyeing equipment of this utility model;

[0017] Figure 3 This is a circuit diagram of the voltage adjustment circuit of this utility model;

[0018] Figure 4This is a circuit diagram of the staining reagent detection circuit of this utility model. Detailed Implementation

[0019] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains; the terminology used herein in the specification is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention; the terms "comprising" and "having," and any variations thereof, in the specification, claims, and accompanying drawings of this invention are intended to cover non-exclusive inclusion. The terms "first," "second," etc., in the specification, claims, or accompanying drawings of this invention are used to distinguish different objects, not to describe a particular order.

[0020] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of the present invention. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0021] To enable those skilled in the art to better understand the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings.

[0022] like Figures 1-4As shown, this utility model provides a reagent type detection circuit for an automatic staining device, including a power supply, a voltage adjustment circuit, an automatic staining device main control circuit, a staining reagent detection circuit, and a touch screen. The output terminal of the power supply is connected to the voltage adjustment circuit and the staining reagent detection circuit. The output terminal of the voltage adjustment circuit is connected to the automatic staining device main control circuit. The output terminal of the automatic staining device main control circuit is connected to the input terminal of the staining reagent detection circuit. The output terminal of the staining reagent detection circuit is connected to the input terminal of the automatic staining device main control circuit for feedback. The automatic staining device main control circuit is also connected to the touch screen for communication. The touch screen can input information via touch. The staining reagent detection circuit is equipped with multiple staining reagent type detection sensors. In this embodiment, the main control circuit of the automatic staining equipment can control the staining reagent detection circuit to detect the type of staining reagent in the staining reagent storage component of the automatic staining equipment and feed it back to the main control circuit of the automatic staining equipment, which is then transmitted to the touch screen for display. This enables automatic identification of the type of staining reagent in the staining reagent storage component, thereby ensuring that the correct type of staining reagent is added to the staining tank. No manual operation is required, which saves time and effort and reduces the risk of errors. This significantly improves the production efficiency and product quality of microscope slides for biological tissue sample sections, and enhances the user experience.

[0023] like Figure 2 As shown, the main control circuit of the automatic dyeing equipment includes a main control chip U5 and a fuse resistor FB13. The main control chip U5 is an STM32F407ZGT6 with 140 pins. Pin 32 of the main control chip U5 is connected to pin 33 and one end of the fuse resistor FB13. The other end of the fuse resistor FB13 is connected to the output of the voltage adjustment circuit. Pins 110, 114, 115, 93, 98, 99, 90, and 91 of the main control chip U5 are connected to the dyeing reagent detection circuit, and pins 96, 97, and 113 of the main control chip U5 are connected to the touch screen display. In this embodiment, the main control circuit of the automatic dyeing equipment is used to control the dyeing reagent detection circuit to detect the type of dyeing reagent in the dyeing reagent storage component of the automatic dyeing equipment and feed it back to the main control circuit for display on the touch screen display. It is also used to control the dyeing reagent storage component of the automatic dyeing equipment to add the correct type of dyeing reagent to the dyeing tank.

[0024] like Figure 3As shown, the voltage regulation circuit includes a voltage regulator chip U4, a diode D5, a resistor R16, a capacitor CT3, and a capacitor C28. The voltage regulator chip U4 is model LM1085IS-3.3 / NOPB, and it has four pins. Pin 3 of U4 is connected to one end of capacitor CT3, one end of capacitor C28, and the output terminal of the power supply. Pin 2 of U4 is connected to pin 4 of U4, one end of resistor R16, and the other end of fuse resistor FB13. The other end of resistor R16 is connected to the diode D5. The positive terminal of diode D5 is connected to the ground. Pin 1 of voltage regulator chip U4, the other end of capacitor CT3, the other end of capacitor C28, and the negative terminal of diode D5 are grounded. The voltage adjustment circuit also includes capacitors CT4, C29, C254, and C255. Pin 2 of voltage regulator chip U4 is connected to one end of capacitor CT4, one end of capacitor C29, one end of capacitor C254, and one end of capacitor C255. The other ends of capacitors CT4, C29, C254, and C255 are grounded. In this embodiment, the voltage adjustment circuit is used to power the main control circuit of the automatic dyeing equipment.

[0025] like Figure 4As shown, the staining reagent detection circuit includes a staining reagent model detection sensor U18D, diode D10, staining reagent model detection sensor U18E, diode D11, staining reagent model detection sensor U18F, diode D12, staining reagent model detection sensor U18A, diode D13, staining reagent model detection sensor U18B, diode D14, staining reagent model detection sensor U18C, and diode D15. One end of the staining reagent model detection sensor U18D is connected to the negative terminal of diode D10 and the main control... Pin 110 of the main control chip U5 is connected to the main control chip. The anode of diode D10 is connected to the output terminal of the power supply. One end of the staining reagent model detection sensor U18E is connected to the cathode of diode D11 and pin 114 of the main control chip U5. The anode of diode D11 is connected to the output terminal of the power supply. One end of the staining reagent model detection sensor U18F is connected to the cathode of diode D12 and pin 115 of the main control chip U5. The anode of diode D12 is connected to the output terminal of the power supply. One end of the staining reagent model detection sensor U18A... One end of the staining reagent model detection sensor U18B is connected to the negative terminal of diode D13 and pin 93 of the main control chip U5, and the positive terminal of diode D13 is connected to the output terminal of the power supply. One end of the staining reagent model detection sensor U18C is connected to the negative terminal of diode D15 and pin 99 of the main control chip U5, and the positive terminal of diode D15 is connected to the output terminal of the power supply. (Staining reagent detection...) The circuit also includes a dyeing reagent type detection sensor U19D, diode D16, dyeing reagent type detection sensor U19E, and diode D17. One end of the dyeing reagent type detection sensor U19D is connected to the negative terminal of diode D16 and pin 90 of the main control chip U5, while the positive terminal of diode D16 is connected to the output terminal of the power supply. One end of the dyeing reagent type detection sensor U19E is connected to the negative terminal of diode D17 and pin 91 of the main control chip U5, while the positive terminal of diode D17 is connected to the output terminal of the power supply. The dyeing reagent type detection sensors U18A, U18B, U18C, U18D, U18E, U18F, U19D, and U19E are all model number SN74LS14DR. In this embodiment, the staining reagent detection circuit is used to detect the type of staining reagent in the staining reagent temporary storage component of the automatic staining equipment and feed it back to the main control circuit of the automatic staining equipment.

[0026] As can be seen from the above, this utility model provides a reagent type detection circuit for an automatic staining device. By setting up a power supply, voltage adjustment circuit, main control circuit of the automatic staining device, staining reagent detection circuit, and touch screen in a mutually cooperating manner, the main control circuit of the automatic staining device can control the staining reagent detection circuit to detect the staining reagent type in the staining reagent temporary storage component of the automatic staining device and feed it back to the main control circuit of the automatic staining device, which is then transmitted to the touch screen for display. This enables automatic identification of the staining reagent type in the staining reagent temporary storage component, thereby ensuring that the correct type of staining reagent is added to the staining tank. No manual operation is required, saving time and effort and reducing errors. This significantly improves the production efficiency and product quality of microscope slides for biological tissue sample sections, enhances the user experience, and solves the problem of time-consuming, labor-intensive, and error-prone manual addition of staining reagents during staining operations in existing biological tissue sample section staining devices.

[0027] The specific embodiments described above are preferred embodiments of this utility model, and are not intended to limit the specific scope of this utility model. The scope of this utility model includes but is not limited to the specific embodiments described above. All equivalent changes made in accordance with this utility model are within the protection scope of this utility model.

Claims

1. A reagent type detection circuit for an automated staining device, characterized in that: The device includes a power supply, a voltage adjustment circuit, an automatic dyeing equipment main control circuit, a dyeing reagent detection circuit, and a touch screen. The output of the power supply is connected to the voltage adjustment circuit and the dyeing reagent detection circuit. The output of the voltage adjustment circuit is connected to the automatic dyeing equipment main control circuit. The output of the automatic dyeing equipment main control circuit is connected to the input of the dyeing reagent detection circuit. The output of the dyeing reagent detection circuit is also connected to the input of the automatic dyeing equipment main control circuit. The automatic dyeing equipment main control circuit is also communicatively connected to the touch screen, which allows for touch input of information. The dyeing reagent detection circuit contains multiple dyeing reagent model detection sensors. The automatic dyeing equipment main control circuit can control the dyeing reagent detection circuit to detect the dyeing reagent model in the dyeing reagent temporary storage component of the automatic dyeing equipment and feed it back to the automatic dyeing equipment main control circuit for display on the touch screen.

2. The reagent model detection circuit for an automated staining device according to claim 1, characterized in that: The main control circuit of the automatic dyeing equipment includes a main control chip U5 and a fuse resistor FB13. The main control chip U5 has 140 pins. Pin 32 of the main control chip U5 is connected to pin 33 of the main control chip U5 and one end of the fuse resistor FB13. The other end of the fuse resistor FB13 is connected to the output terminal of the voltage adjustment circuit. Pins 110, 114, 115, 93, 98, 99, 90, and 91 of the main control chip U5 are connected to the dyeing reagent detection circuit. Pins 96, 97, and 113 of the main control chip U5 are connected to the touch screen display.

3. The reagent model detection circuit for an automated staining device according to claim 2, characterized in that: The voltage regulation circuit includes a voltage regulator chip U4, a diode D5, a resistor R16, a capacitor CT3, and a capacitor C28. The voltage regulator chip U4 has four pins. The third pin of the voltage regulator chip U4 is connected to one end of the capacitor CT3, one end of the capacitor C28, and the output terminal of the power supply. The second pin of the voltage regulator chip U4 is connected to the fourth pin of the voltage regulator chip U4, one end of the resistor R16, and the other end of the fuse resistor FB13. The other end of the resistor R16 is connected to the positive terminal of the diode D5. The first pin of the voltage regulator chip U4, the other end of the capacitor CT3, the other end of the capacitor C28, and the negative terminal of the diode D5 are grounded.

4. The reagent model detection circuit for an automated staining device according to claim 3, characterized in that: The voltage adjustment circuit also includes capacitors CT4, C29, C254, and C255. The second pin of the voltage regulator chip U4 is connected to one end of capacitor CT4, one end of capacitor C29, one end of capacitor C254, and one end of capacitor C255. The other ends of capacitors CT4, C29, C254, and C255 are grounded.

5. The reagent model detection circuit for an automated staining device according to claim 4, characterized in that: The staining reagent detection circuit includes a staining reagent model detection sensor U18D, diode D10, staining reagent model detection sensor U18E, diode D11, staining reagent model detection sensor U18F, diode D12, staining reagent model detection sensor U18A, diode D13, staining reagent model detection sensor U18B, diode D14, staining reagent model detection sensor U18C, and diode D15. One end of the staining reagent model detection sensor U18D is connected to the negative terminal of diode D10 and pin 110 of the main control chip U5, while the positive terminal of diode D10 is connected to the output terminal of the power supply. One end of the staining reagent model detection sensor U18E is connected to the negative terminal of diode D11 and pin 114 of the main control chip U5, while the positive terminal of diode D11 is connected to the output terminal of the power supply. One end of the staining reagent type detection sensor U18F is connected to the negative terminal of diode D12 and pin 115 of the main control chip U5, and the positive terminal of diode D12 is connected to the output terminal of the power supply. One end of the staining reagent type detection sensor U18A is connected to the negative terminal of diode D13 and pin 93 of the main control chip U5, and the positive terminal of diode D13 is connected to the output terminal of the power supply. One end of the staining reagent type detection sensor U18B is connected to the negative terminal of diode D14 and pin 98 of the main control chip U5, and the positive terminal of diode D14 is connected to the output terminal of the power supply. One end of the staining reagent type detection sensor U18C is connected to the negative terminal of diode D15 and pin 99 of the main control chip U5, and the positive terminal of diode D15 is connected to the output terminal of the power supply.

6. The reagent model detection circuit for an automated staining device according to claim 5, characterized in that: The staining reagent detection circuit also includes a staining reagent type detection sensor U19D, a diode D16, a staining reagent type detection sensor U19E, and a diode D17. One end of the staining reagent type detection sensor U19D is connected to the negative terminal of the diode D16 and pin 90 of the main control chip U5, and the positive terminal of the diode D16 is connected to the output terminal of the power supply. One end of the staining reagent type detection sensor U19E is connected to the negative terminal of the diode D17 and pin 91 of the main control chip U5, and the positive terminal of the diode D17 is connected to the output terminal of the power supply.

7. The reagent model detection circuit for an automated staining device according to claim 6, characterized in that: The main control chip U5 is an STM32F407ZGT6, the voltage regulator chip U4 is an LM1085IS-3.3 / NOPB, and the staining reagent model detection sensors U18A, U18B, U18C, U18D, U18E, U18F, U19D, and U19E are all SN74LS14DR.

Images