Biological catalysis device for stevioside

Abstract

The utility model discloses a biological catalysis device for stevioside, which comprises a reaction tank communicated with an enzyme storage tank, a plate-and-frame filter and a macroporous adsorption resin system. The macroporous adsorption resin system is respectively communicated with a pure water storage tank and a high-alcohol desorption agent storage tank; a high-alcohol desorption solution outlet and a water washing solution outlet of the macroporous adsorption resin system are sequentially communicated with a first concentration tank, a crystallizing tank, a centrifugal machine, a drying tank, a crushing tank and a stevioside storage tank; and a centrifugate outlet of the centrifugal machine is communicated with the second concentration tank and the material recovery tank. According to the device, a solution containing stevioside is synthesized through biological catalysis, and then high-purity stevioside is obtained through a series of post-treatment.

Description

TECHNICAL FIELD

[0001] The utility model relates to a rebaudioside production device, in particular to a rebaudioside biological catalysis device. BACKGROUND

[0002] Rebaudioside is a high-sweetness, zero-calorie sweetener extracted from stevia leaves, which can be used as a food additive. In recent years, the demand for rebaudioside in the domestic and foreign markets has increased dramatically, attracting widespread attention. Currently, there are about 40 sources of stevia known, which have different degrees of sweetness and taste, and with the improvement of separation and analysis technology, new components are reported. They have a common four-ring diterpene mother ring and different glycosylation side chain modifications in chemical structure. Therefore, it is of great significance to generate high-sweetness, better-tasting rebaudioside from low-sweetness glycosides through biological catalysis, but there is less research on the device for preparing rebaudioside through biological catalysis. SUMMARY

[0003] The technical problem to be solved by the utility model is that, in view of the deficiencies in the prior art, a rebaudioside biological catalysis device is provided, which synthesizes a solution containing rebaudioside through biological catalysis, and then obtains high-purity rebaudioside through a series of post-processing.

[0004] To solve the above technical problems, the technical scheme of the utility model is as follows:

[0005] A rebaudioside biological catalysis device, comprising a reaction tank, the reaction tank being connected with an enzyme storage tank, a plate and frame filter, and a macroporous adsorption resin system; the macroporous adsorption resin system being connected with a pure water storage tank and a high alcohol eluent storage tank respectively, the high alcohol eluent outlet and the water washing liquid outlet of the macroporous adsorption resin system being connected with a first concentration tank, a crystallization tank, a centrifuge, a drying tank, a crushing tank, and a rebaudioside storage tank in sequence; and the centrifugal liquid outlet of the centrifuge being connected with a second concentration tank and a material recovery tank.

[0006] Preferably, the macroporous adsorption resin system comprises at least two macroporous adsorption resin columns connected in series.

[0007] Preferably, the top of the macroporous adsorption resin column is provided with a distribution disc connected with a feeding pipeline.

[0008] Preferably, the water top alcohol solution outlet of the macroporous adsorption resin system is connected with the high alcohol eluent storage tank through a buffer tank.

[0009] Preferably, the crystallization tank is provided with a stirring device, the stirring device comprising a stirring shaft arranged in the crystallization tank, the stirring shaft being connected with a stirring motor arranged at the top of the crystallization tank, the stirring shaft being provided with main stirring blades, and the bottom of the stirring shaft being provided with auxiliary stirring blades matched with the shape of the bottom of the crystallization tank.

[0010] Preferably, the grinding tank is provided with a grinding mechanism, which includes a rotating shaft disposed inside the grinding tank and toothed grinding blades disposed on the rotating shaft. The rotating shaft is connected to a grinding motor disposed at the top of the grinding tank.

[0011] Due to the adoption of the above technical solution, the beneficial effects of this utility model are:

[0012] This invention provides a biocatalytic device for steviol glycosides, comprising a reaction vessel connected to an enzyme storage tank, a plate and frame filter, and a macroporous adsorption resin system. The macroporous adsorption resin system is connected to a pure water storage tank and a high-alcohol eluent storage tank. The high-alcohol eluent outlet and the washing liquid outlet of the macroporous adsorption resin system are sequentially connected to a first concentration tank, a crystallization tank, a centrifuge, a drying tank, a pulverizing tank, and a steviol glycoside storage tank. The centrifuge's centrifugal liquid outlet is connected to a second concentration tank and the material. The above device prepares steviol glycosides through biocatalysis, and the unreacted substrates in the production process are recovered and reused, greatly reducing energy consumption.

[0013] The top of the macroporous adsorption resin column of this device is equipped with a material distribution plate that is connected to the feed pipe. The material distribution plate makes the feed more uniform, avoids segregation, and improves the adsorption efficiency of steviol glycosides.

[0014] The outlet of the water-to-alcohol solution in the macroporous adsorption resin system of this device is connected to a high-alcohol desorption agent storage tank through a buffer tank. The water-to-alcohol solution enters the buffer tank for temporary storage during the process, and after adjusting the alcohol concentration, it can be used as a high-alcohol desorption agent, further reducing energy consumption.

[0015] The crystallization tank of this device is equipped with a stirring device, which includes a stirring shaft installed inside the crystallization tank. The stirring shaft is connected to a stirring motor installed at the top of the crystallization tank. The stirring shaft is equipped with main stirring blades, and the bottom of the stirring shaft is equipped with auxiliary stirring blades that are adapted to the shape of the bottom of the crystallization tank. The above configuration improves the crystallization efficiency to a certain extent.

[0016] This device has a pulverizing mechanism inside its pulverizing tank. The pulverizing mechanism includes a rotating shaft inside the pulverizing tank and toothed pulverizing blades mounted on the shaft. The rotating shaft is connected to a pulverizing motor located at the top of the pulverizing tank. This configuration pulverizes the product to a suitable particle size, thereby improving product quality. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a schematic diagram of the structure of Embodiment 1 of this utility model;

[0019] In the diagram, 1 is the reaction vessel; 2 is the enzyme storage tank; 3 is the plate and frame filter; 4 is the pure water storage tank; 5 is the high-alcohol desorption agent storage tank; 6 is the first concentration tank; 7 is the crystallization tank; 8 is the centrifuge; 9 is the drying tank; 10 is the pulverizing tank; 11 is the steviol glycoside storage tank; 12 is the second concentration tank; 13 is the material recovery tank; 14 is the macroporous adsorption resin column; 15 is the material distribution tray; 16 is the buffer tank; 17 is the stirring shaft; 18 is the stirring motor; 19 is the main stirring blade; 20 is the auxiliary stirring blade; 21 is the rotating shaft; 22 is the toothed pulverizing blade; and 23 is the pulverizing motor. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present utility model.

[0021] Example 1

[0022] like Figure 1 As shown, a biocatalytic device for steviol glycosides includes a reaction tank 1, which is connected to an enzyme storage tank 2, a plate and frame filter 3, and a macroporous adsorption resin system. The macroporous adsorption resin system is connected to a pure water storage tank 4 and a high-alcohol eluent storage tank 5. The high-alcohol eluent outlet and the washing liquid outlet of the macroporous adsorption resin system are sequentially connected to a first concentration tank 6, a crystallization tank 7, a centrifuge 8, a drying tank 9, a pulverizing tank 10, and a steviol glycoside storage tank 11. The centrifugal liquid outlet of the centrifuge 8 is connected to a second concentration tank 12 and a material recovery tank 13.

[0023] In this embodiment, the macroporous adsorption resin system includes at least two macroporous adsorption resin columns 14 connected in series, and more preferably includes three macroporous adsorption resin columns connected in series, to ensure that steviol glycosides in the feed solution are fully adsorbed and to improve the production efficiency of the product.

[0024] In this embodiment, the top of the macroporous adsorption resin column 14 is provided with a distribution plate 15 that is connected to the feed pipe. The distribution plate 15 allows the liquid to enter the macroporous adsorption resin column 14 evenly, avoiding segregation and improving adsorption efficiency.

[0025] In this embodiment, the water-to-alcohol solution outlet of the macroporous adsorption resin system is connected to the high-alcohol desorption agent storage tank 5 via a buffer tank 16. The water-to-alcohol solution produced enters the buffer tank 16 and, after adjusting the alcohol concentration, can be used as the high-alcohol desorption agent.

[0026] In the embodiment, the crystallization tank 7 is provided with a stirring device, which comprises a stirring shaft 17 arranged in the crystallization tank 7, the stirring shaft 17 being connected with a stirring motor 18 arranged at the top of the crystallization tank 7, the stirring shaft 17 being provided with main stirring blades 19, and the bottom of the stirring shaft 17 being provided with auxiliary stirring blades 20 which are adapted to the shape of the bottom of the crystallization tank 7. The cooperation of the main stirring blades 19 and the auxiliary stirring blades 20 improves the crystallization efficiency.

[0027] In the embodiment, the crushing tank 10 is provided with a crushing mechanism, which comprises a rotating shaft 21 arranged in the crushing tank 10 and tooth-shaped crushing blades 22 arranged on the rotating shaft 21, the rotating shaft 21 being connected with a crushing motor 23 arranged at the top of the crushing tank 10; the above-mentioned arrangement is used to crush the dried products, so that the products reach the appropriate particle size and the product quality is improved.

[0028] The above description of the disclosed embodiments enables those skilled in the art to carry out or use the present application. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present application. Therefore, the present application will not be limited to the embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A biocatalytic device for steviol glycosides comprising a reaction tank, characterized in that: The reaction tank is communicated with an enzyme storage tank, a plate and frame filter, and a macroporous adsorption resin system; the macroporous adsorption resin system is respectively communicated with a pure water storage tank and a high-alcohol eluent storage tank; the high-alcohol eluent outlet and the water washing liquid outlet of the macroporous adsorption resin system are sequentially communicated with a first concentration tank, a crystallization tank, a centrifugal machine, a drying tank, a crushing tank, and a stevioside storage tank; the centrifugal liquid outlet of the centrifugal machine is communicated with a second concentration tank and a material recovery tank.

2. The biocatalytic device of claim 1, wherein: The macroporous adsorption resin system comprises at least two macroporous adsorption resin columns connected in series.

3. A biocatalytic device for steviol glycosides according to claim 2, characterized in that: A top portion of the macroporous adsorption resin column is provided with a distribution plate communicated with a feeding pipeline.

4. The biocatalytic device of claim 1, wherein: The water top alcohol solution outlet of the macroporous adsorption resin system is communicated with the high-alcohol eluent storage tank through a buffer tank.

5. The biocatalytic device of claim 1, wherein: The crystallization tank is provided with a stirring device, which comprises a stirring shaft arranged in the crystallization tank and connected with a stirring motor arranged at a top portion of the crystallization tank, and a main stirring blade arranged on the stirring shaft and an auxiliary stirring blade arranged at a bottom portion of the stirring shaft and matched with a shape of a bottom portion of the crystallization tank.

6. The biocatalytic device of steviol glycosides according to claim 1, characterized in that: The crushing tank is provided with a crushing mechanism, which comprises a rotating shaft arranged in the crushing tank and a tooth-shaped crushing blade arranged on the rotating shaft, and the rotating shaft is connected with a crushing motor arranged at a top portion of the crushing tank.

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