Experimental device for adherent cell overload research
By designing an adjustable-angle experimental device and shock-absorbing materials, the problem of the inability of existing equipment to precisely adjust the angle of cell culture flasks was solved, enabling stable overload studies of adherent cells, reducing the complexity of experimental operations and cleaning and disinfection, and meeting the needs of aerospace medical research.
Patent Information
- Application Number
- CN202520487129.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-19
- Publication Date
- 2026-02-27
- Estimated Expiration
- 2035-03-19
AI Technical Summary
Existing benchtop centrifuges cannot precisely adjust the angle of cell culture flasks, resulting in uneven acceleration and shear force interference to the cells, and there is a risk of leakage. They cannot meet the needs of research on accelerated overload for adherent cell metabolism.
An experimental device was designed, comprising a chassis, an L-shaped backplate, a support plate, and an angle adjustment assembly. The angle of the cell culture flask can be precisely adjusted by using an angle adjustment knob and a fine-tuning knob. It is equipped with shock-absorbing materials and disposable covers to reduce the impact of vibration and the risk of cross-contamination.
This approach enables precise overload studies of adherent cells, reduces the complexity of experimental procedures and the workload of cleaning and disinfection, lowers research costs, and provides a stable experimental environment.
Smart Images
Figure CN223951040U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the field of aviation medicine, physiology, cell biology experiment equipment, and specifically relates to an experimental device for adherent cell overload research. BACKGROUND
[0002] At present, there are overload devices for researching the physiological influence of acceleration on animals or desktop cell centrifuge products in China, which are mainly used for cell collection, washing, gradient separation and purification of cells and other substances, separation of subcellular components and macromolecular substances and sample concentration. A device specially researching the influence of acceleration overload +Gz on adherent cell metabolism has not been developed. In addition, the only self-researched aerospace acceleration desktop centrifuge in China is based on the principle of ordinary high-speed desktop centrifuge to transform the mold, and has not been commercialized. It also does not meet the research needs. Although the mold can vertically place the centrifuge bottles and culture plates, it has limitations due to the short rotating arm of the desktop centrifuge. The cell culture bottles placed vertically are too close to the center of the rotating shaft, resulting in different directions and uneven (different sizes) acceleration forces acting on the cells at the upper, middle and lower sections of the same culture bottle. Moreover, the angle of the bottle body cannot be accurately adjusted to change the angle of force, so for the test cells, in addition to being affected by uneven acceleration, they are also disturbed by shear force. In addition, such centrifuges are prone to liquid leakage during use due to the vertical placement of the culture plates, which requires that the test cell culture plates be sealed, which can easily interfere with the experimental results due to lack of oxygen. Therefore, the above equipment cannot meet the needs of +Gz for adherent cell metabolism experiments.
[0003] For a specific group, such as fighter pilots during takeoff or aerial stunt flying, cells in the body are simultaneously affected by shear force and overload. A large amount of aviation medical research data shows that for some specific diseases, the influence of overload on human tissues and cells is more prominent than that of shear force. In order to accurately research the influence of acceleration overload, especially +Gz on adherent cell metabolism, and considering cost savings, the present application discloses an experimental device for adherent cell overload research. UTILITY MODEL CONTENT
[0004] The utility model aims to provide an experimental device for adherent cell overload research to solve the problems raised in the background art.
[0005] To achieve the above-mentioned purpose, the utility model provides the following technical scheme:
[0006] An experimental device for adherent cell overload research, including a base plate, the upper end of the base plate is rotatably connected with a rotatable L-shaped back plate, the upper end of the base plate is fixedly connected with a support plate, the outer wall of the end of the L-shaped back plate close to the support plate is fixedly connected with a support rod for adjusting the angle, the outer wall of the end of the support plate away from the L-shaped back plate is fixedly connected with a fixed rod, and the fixed rod is fixed to the outer surface of the support rod; and a main body box is installed outside the base plate, an angle adjusting assembly for adjusting the angle of the L-shaped back plate is installed on the outer wall of the main body box.
[0007] The outer wall of the end of the L-shaped back plate away from the support plate is movably connected with a first clamp and a second clamp for fixing a cell culture bottle.
[0008] As a further scheme of the present application, an opening for the movement of the support rod is formed in the middle of the support plate, and the part of the support rod passing through the opening of the support plate is fixedly connected with the fixed rod through a clamp.
[0009] As a further scheme of the present application, the angle adjusting assembly includes an angle adjusting knob turntable, the angle of the support rod of the L-shaped back plate can be adjusted through the angle adjusting knob turntable, so as to drive the L-shaped back plate to reach a preset angle, and a fine adjustment knob is rotatably connected to the outer wall of the angle adjusting knob turntable.
[0010] As a further scheme of the present application, a plurality of clamping grooves and a sliding groove for adjusting the angle are formed in the outer wall of the support rod, a fixed groove is formed in the outer wall of the fixed rod, and a fixed rail is fixedly connected to the fixed groove, the clamping grooves and the fixed groove are of the same specification, can be fixed through a corresponding clamp, and the sliding groove is slidably connected to the outer wall of the fixed rail.
[0011] As a further scheme of the present application, a tooth groove is formed in the outer wall of the support rod, a poking rod is rotatably connected to the outer wall of the angle adjusting knob turntable, and a linkage shaft is installed in the main body box.
[0012] As a further scheme of the present application, a first gear and a second gear are fixedly connected to the two ends of the linkage shaft, the second gear is engaged with the tooth groove of the support rod, a fine adjustment gear is rotatably connected to the inner wall of the main body box, and the adjusting gear away from the fine adjustment knob is engaged with the first gear.
[0013] Compared with the prior art, the present application has the following advantages:
[0014] 1. The utility model discloses a special design for +Gz overload cell experiment research, which can accurately meet the special needs of such research, can be easily installed and fixed on the panel of the overload device, greatly facilitates the development of cell overload experiment, effectively improves the convenience of experimental operation, has various cell culture bottle culture plate specifications, and shows wide applicability.
[0015] 2. The utility model discloses a shock-absorbing material in the device, which can effectively reduce the adverse effects of vibration on cells during the experiment, create a relatively stable experimental environment for cells, and is equipped with a disposable cover that can be directly discarded after each experiment, reducing the risk of cross contamination and greatly reducing the cleaning and disinfection workload, ensuring the experimental hygiene conditions. In addition, it is more convenient to disassemble and completely disinfect the inner and outer surfaces, can be reused multiple times, effectively saving research costs, and providing an efficient, reliable and economical solution for cell overload research. BRIEF DESCRIPTION OF DRAWINGS
[0016] Fig. 1 It is a structural diagram of an experimental device for adherent cell overload research.
[0017] Fig. 2 It is a structural diagram of the inside of an experimental device for adherent cell overload research.
[0018] Fig. 3 It is another perspective view of the inside of an experimental device for adherent cell overload research.
[0019] Fig. 4 It is a schematic diagram of the support rod of an experimental device for adherent cell overload research.
[0020] Fig. 5 It is a schematic diagram of the support rod and fixed rod of an experimental device for adherent cell overload research.
[0021] Fig. 6 It is an adjustment principle diagram of the support rod of an experimental device for adherent cell overload research.
[0022] In the figure: 1, base; 2, L-shaped back plate; 3, support plate; 4, support rod; 401, sliding groove; 402, clamping groove; 403, tooth groove; 5, fixed rod; 501, fixed rail; 502, fixed groove; 6, rotating shaft; 7, first clamp; 8, second clamp; 9, outer fixator; 10, main body box; 11, pull handle knob; 12, angle adjustment knob turntable; 1201, lever; 1202, linkage shaft; 1203, first gear; 1204, second gear; 13, fine adjustment knob; 1301, fine adjustment gear; 14, handle. DETAILED DESCRIPTION
[0023] With reference to the drawings of the embodiments of the present application, the technical solutions in the embodiments of the present application will be clearly and completely described. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments of the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of the present application.
[0024] Please refer to Figs. 1-6 In the embodiments of the present application, an experimental device for adherent cell overload research comprises a bottom disc 1, the upper end of the bottom disc 1 is rotationally connected with a rotatable L-shaped back plate 2, specifically, the upper end outer wall of the bottom disc 1 is rotationally connected with a rotating shaft 6, the outer wall of the rotating shaft 6 is fixedly connected to the bottom end of the L-shaped back plate 2, when the L-shaped back plate 2 is rotated to a certain angle, in order to support it and ensure its stability, the upper end of the bottom disc 1 is fixedly connected with a support plate 3, specifically, the outer wall of one end of the L-shaped back plate 2 close to the support plate 3 is fixedly connected with a support rod 4 for adjusting the angle, the outer wall of one end of the support plate 3 away from the L-shaped back plate 2 is fixedly connected with a fixed rod 5, and the fixed rod 5 is fixed to the outer surface of the support rod 4, more specifically, the middle of the support plate 3 is provided with an opening for the movement of the support rod 4, the part of the support rod 4 passing through the opening of the support plate 3 is fixedly connected with the fixed rod 5 through a clamp.
[0025] Please refer to Fig. 1 In order to isolate the cell culture bottle on the bottom disc 1 from the external environment during the experiment, a main body box 10 is installed outside the bottom disc 1, specifically, the main body box 10 is made of pressure-resistant, light, and antibacterial material, and the front, two sides, and the upper vertical surface are made of transparent and visible material, so as to facilitate the observation of the state of the cell culture device, the outside of the main body box 10 is matched with consumables, which are made of wear-resistant, transparent, and antibacterial disposable sterilization material, so that after each overload experiment is completed, the disposable cover is thrown away, so as to achieve the relatively clean state of the device surface, reduce the risk of cross contamination, and also reduce the workload of cleaning and disinfection of the device.
[0026] In order to improve the stability of the device during the overload experiment, reduce the influence of the device shaking on the cells, an external fixator 9 is installed on the outside of the main box 10, in order to adjust the angle of the support plate 3 and the support rod 4, an angle adjusting assembly for adjusting the angle of the L-shaped back plate 2 is installed on the outer wall of the main box 10, which includes an angle adjusting knob rotary disc 12, which can adjust the angle of the support rod 4 of the L-shaped back plate 2, so as to drive the L-shaped back plate 2 to reach the preset angle, specifically, the preset angle of the L-shaped back plate 2 includes 2G: 30° (when reaching 2G overweight state, the L-shaped back plate 2 needs to be adjusted by 30°, and the rest is the same), 2.5G: 23.6°, 3G: 19.5°, 3.5G: 16.6°, 4.5G: 12.7°, 6G: 9.6°, 8G: 7.2°;
[0027] In order to further adjust the angle of the L-shaped back plate 2 more carefully, the outer wall of the angle adjusting knob rotary disc 12 is rotatably connected with a fine tuning knob 13, the front end outer wall of the main box 10 is fixedly connected with a pull door knob 11 which opens the outer wall, and in order to facilitate the transfer and handling of the device, the upper end of the main box 10 is fixedly connected with a handle 14 which can handle the device;
[0028] Please refer to Fig. 4 The outer wall of the L-shaped back plate 2 is provided with different specifications of cell culture bottle fixing clamps and holes, which can load T-175, T-75, T-25 and other models of cell culture bottles, and the size of the culture bottle is T-175 cell culture bottle (length (from bottle mouth to bottom 19.6cm) x width 12.4cm x thickness 3.9cm), T-75 cell culture bottle (length (from bottle mouth to bottom 16.2cm) x width 9cm x thickness 3.6cm), T-25 cell culture bottle (length (from bottle mouth to bottom 9.7cm) x width 5.1cm x thickness 2.6cm), in order to support the loaded cell culture bottle, the outer wall of the end of the L-shaped back plate 2 away from the support plate 3 is movably connected with the first clamp 7 and the second clamp 8, the cell culture bottle is placed between the first clamp 7, the second clamp 8 and the L-shaped back plate 2, specifically, the first clamp 7 and the second clamp 8 are staggered, and the notch part of the first clamp 7 faces the outer wall of the L-shaped back plate 2, so as to support the bottom end of the cell culture bottle placed therein, and a damping material is arranged between the L-shaped back plate 2 and the culture bottle, so as to reduce the influence of the vibration generated during the operation of the equipment on the cells;
[0029] Please refer to Fig. 5The outer wall of the support rod 4 is provided with a plurality of clamping grooves 402 and a sliding groove 401 for adjusting the angle, the outer wall of the fixing rod 5 is provided with a fixing groove 502 and is fixedly connected with a fixing rail 501, specifically, the clamping grooves 402 and the fixing grooves 502 are of the same specification and can be fixed by corresponding clamps, the sliding groove 401 is slidingly connected to the outer wall of the fixing rail 501, the support rod 4 is adjusted to a suitable position by a knob on the angle adjusting knob disc 12, and the clamping grooves 402 and the fixing grooves 502 corresponding to the present moment are connected together by clamps, so that the position of the support rod 4 is stably adjusted, and the outer wall of the support rod 4 is provided with a gear slot 403;
[0030] Please refer to Fig. 6 The outer wall of the angle adjusting knob disc 12 is rotatably connected with a pulling rod 1201, the inside of the main body box 10 is provided with a linkage shaft 1202, specifically, the linkage shaft 1202 is fixedly connected with the pulling rod 1201, more specifically, the inner wall of the bottom disc 1 is fixedly connected with a support column, the linkage shaft 1202 is rotatably connected to the inside of the support column, the two ends of the linkage shaft 1202 are fixedly connected with a first gear 1203 and a second gear 1204, the first gear 1203 is fixedly connected to one end close to the angle adjusting knob disc 12, the second gear 1204 is fixedly connected to one end of the linkage shaft 1202 away from the angle adjusting knob disc 12, the second gear 1204 is engaged with the gear slot 403 of the support rod 4, and the linkage shaft 1202 is rotated by rotating the pulling rod 1201, so that the second gear 1204 drives the support rod 4 to rotate;
[0031] The inner wall of the main body box 10 is rotatably connected with a fine adjustment gear 1301, specifically, the outer wall of the fine adjustment gear 1301 is provided with two kinds of adjustment gears with different sizes, one end of the adjustment gear close to the fine adjustment knob 13 is engaged with the fine adjustment knob 13, one end of the adjustment gear away from the fine adjustment knob 13 is engaged with the first gear 1203, and the diameter of the adjustment gear engaged with the fine adjustment knob 13 is smaller than that of the adjustment gear engaged with the first gear 1203, the fine adjustment gear 1301 is rotated by rotating the fine adjustment knob 13, the fine adjustment gear 1301 further drives the first gear 1203 and the linkage shaft 1202 to rotate, and since the tooth gap specification of the fine adjustment knob 13 is smaller, the linkage shaft 1202 is adjusted only in a small degree each time the fine adjustment knob 13 is rotated, so that the angle of the support rod 4 can be more accurately adjusted.
[0032] The working principle of the utility model is:
[0033] The utility model discloses when using, through angle adjusting knob turntable 12 can adjust the angle of the support pole 4 of L type backboard 2, thereby drive L type backplate 2 reach the preset angle, simultaneously place cell culture bottle in the first clamp 7 and second clamp 8 and the middle of L type backplate 2, when the angle of L type backplate 2 changes, the cell culture bottle in it will drive the inclination of different angle, thereby make the cell in cell culture bottle interior can carry out overload experiment according to the angle of anticipation, and through clamp to connect together respectively with the fixed groove 502 of card slot 402 corresponding at this time, thereby reach the stable effect to the position of support pole 4 after adjusting,
[0034] The device is provided with damping materials, which can effectively reduce the adverse effects of oscillation on cells during the experiment, create a relatively stable experimental environment for the cells, and is matched with a disposable cover, which can be directly discarded after each experiment, thereby reducing the risk of cross contamination and greatly reducing the cleaning and disinfection workload, ensuring the experimental hygiene conditions. In addition, it is more convenient to disassemble, facilitating complete disinfection of the inner and outer surfaces, and can be repeatedly used.
[0035] The above is only a preferred specific embodiment of the utility model, but the protection scope of the utility model is not limited to this. Any person skilled in the art can make equivalent replacements or changes according to the technical scheme and the utility model concept of the utility model within the technical range disclosed by the utility model, which should be covered within the protection scope of the utility model.
Claims
1. An experimental device for the study of the overload of adherent cells, comprising a base plate (1), characterized in that, The upper end of the chassis (1) is rotatably connected with a rotatable L-shaped back plate (2), the upper end of the chassis (1) is fixedly connected with a support plate (3), one end of the L-shaped back plate (2) close to the support plate (3) is fixedly connected with a support rod (4) for adjusting the angle, the outer wall of the end of the support plate (3) away from the L-shaped back plate (2) is fixedly connected with a fixed rod (5), and the fixed rod (5) is fixed to the outer surface of the support rod (4); and a main body box (10) is installed outside the chassis (1), an angle adjusting assembly for adjusting the angle of the L-shaped back plate (2) is installed on the outer wall of the main body box (10). The outer wall of the end of the L-shaped back plate (2) away from the support plate (3) is movably connected with a first clamp (7) and a second clamp (8) for fixing a cell culture bottle.
2. The experimental device for the overload study of the adherent cells according to claim 1, characterized in that, An opening for the movement of the support rod (4) is formed in the middle of the support plate (3), and the part of the support rod (4) penetrating through the opening of the support plate (3) is fixedly connected with the fixed rod (5) through a clamp.
3. The experimental device for the overload study of the adherent cells according to claim 1, characterized in that, The angle adjusting assembly comprises an angle adjusting knob rotary disc (12), the angle of the support rod (4) of the L-shaped back plate (2) can be adjusted through the angle adjusting knob rotary disc (12), so as to drive the L-shaped back plate (2) to reach a preset angle, and a fine adjustment knob (13) is rotatably connected to the outer wall of the angle adjusting knob rotary disc (12).
4. The experimental setup for the overloading study of adherent cells according to claim 1, wherein, A plurality of clamping grooves (402) and a sliding groove (401) for adjusting the angle are formed in the outer wall of the support rod (4), a fixed groove (502) is formed in the outer wall of the fixed rod (5) and a fixed rail (501) is fixedly connected thereto, the clamping grooves (402) and the fixed grooves (502) are of the same specification and can be fixed through corresponding clamps, and the sliding groove (401) is slidably connected to the outer wall of the fixed rail (501).
5. The experimental setup for the overloading study of adherent cells according to claim 3, wherein, A tooth groove (403) is formed in the outer wall of the support rod (4), a pulling rod (1201) is rotatably connected to the outer wall of the angle adjusting knob rotary disc (12), and a linkage shaft (1202) is installed in the main body box (10).
6. An experimental device for use in overloading studies of adherent cells according to claim 5, characterized in that First and second gears (1203) and (1204) are fixedly connected to the two ends of the linkage shaft (1202), the second gear (1204) is engaged with the tooth groove (403) of the support rod (4), a fine adjustment gear (1301) is rotatably connected to the inner wall of the main body box (10), and the adjusting gear away from the fine adjustment knob (13) is engaged with the first gear (1203).