Arginine extraction device

Abstract

The utility model discloses an arginine extraction device which realizes efficient and continuous production based on a multi-stage membrane separation technology. The method comprises the following steps: firstly, removing thalli and large-particle impurities from fermentation liquor through a microfiltration membrane'internal pressure method ', and returning retentate back to a pretreatment section; the permeate enters an ultrafiltration membrane to separate soluble protein and polysaccharide by an internal pressure method; after being temporarily stored in the transfer storage tank, the ultrafiltration clear liquid is conveyed to a nanofiltration membrane through a booster pump to remove small organic molecules through an external pressure method, then electrodialysis is carried out to remove inorganic salt ions, and retentate is subjected to closed-loop recycling. And adsorbing pigments and heavy metals in the purified liquid through activated carbon, filtering and removing carbon through a plate frame, feeding the purified liquid into a crystallizing tank, and centrifuging and drying to obtain a high-purity arginine product. According to the utility model, microfiltration-ultrafiltration-nanofiltration stepped separation is combined with electrodialysis, so that the use of chemical reagents is reduced; the resource efficiency is improved through recycling of intercepted substances, continuous production is guaranteed through the design of a transfer storage tank and a booster pump, membrane separation and a traditional purification process are integrated, and high efficiency and environment friendliness are achieved.

Description

Technical Field

[0001] This utility model relates to the technical field of arginine production equipment, and in particular to an arginine extraction device. Background Technology

[0002] Arginine, an important basic amino acid, is widely used in the pharmaceutical, food, and cosmetic industries. Traditional extraction processes typically employ methods such as ion exchange resin adsorption, solvent extraction, or evaporation crystallization, which have the following drawbacks:

[0003] Complex process: Multiple steps result in long cycle time and high energy consumption;

[0004] Limited purity: It is difficult to effectively remove cell debris, large molecular proteins and inorganic salt impurities from the fermentation broth;

[0005] Severe pollution: Resin regeneration generates a large amount of wastewater, and solvent use creates an environmental burden.

[0006] In recent years, membrane separation technology has attracted attention due to its high efficiency and environmental friendliness. However, single membrane technologies cannot simultaneously solve problems such as narrow retention range and rapid membrane fouling. Existing technologies employ ultrafiltration-nanofiltration combined processes, but still suffer from drawbacks such as high conductivity and low crystallization yield.

[0007] Therefore, in view of the shortcomings of the existing technology, it is necessary to design an arginine extraction device to solve the above problems.

[0008] It should be noted that the above introduction to the technical background is only for the purpose of providing a clear and complete explanation of the technical solution of this utility model and facilitating the understanding of those skilled in the art. It should not be assumed that the above content is known to those skilled in the art simply because it has been described in the background section of this utility model. Utility Model Content

[0009] To overcome the shortcomings of the existing technology, the present invention discloses an arginine extraction device to solve the problems of low efficiency, insufficient purity and excessive wastewater pollution in traditional processes.

[0010] This utility model discloses an arginine extraction device, comprising:

[0011] The first filter is divided into a first inner chamber and a first outer chamber by a microfiltration membrane; the inlet end of the first inner chamber is connected to a fermentation broth conveying pipe, which is used to convey the arginine fermentation broth from the pretreatment section to the first inner chamber; the outlet end of the first inner chamber is connected to a recovery pipe; the microfiltration membrane is used to separate the bacterial residue and large particulate impurities in the fermentation broth using an internal pressure method, and the retained material is returned to the pretreatment section through the recovery pipe;

[0012] The second filter is divided into a second inner chamber and a second outer chamber by an ultrafiltration membrane; the inlet end of the second inner chamber is connected to the outlet end of the first outer chamber, and the outlet end of the second inner chamber is connected to a recovery pipeline; the fermentation broth is separated by ultrafiltration membrane using an internal pressure method to separate macromolecular impurities, such as soluble proteins and polysaccharides, and the retentate is returned to the pretreatment section through the recovery pipeline.

[0013] The transfer storage tank, whose inlet is connected to the outlet of the second external cavity, is used to transfer and store the fermentation broth output from the second filter, ensuring continuous feeding.

[0014] The third filter is divided into a third inner chamber and a third outer chamber by a nanofiltration membrane. The inlet of the third outer chamber is connected to the outlet of the transfer tank via a booster pump, which is used to regulate the input pressure of the fermentation broth. The outlet of the third outer chamber is connected to a recovery pipeline. The fermentation broth is separated into small organic molecules by the nanofiltration membrane using an external pressure method. The nanofiltration membrane of the external pressure method is more suitable for high-pressure operation and can improve the nanofiltration efficiency. The filtrate is returned to the pretreatment section through the recovery pipeline.

[0015] The electrodialysis equipment has its inlet end connected to the outlet end of the third inner chamber, and its sewage outlet end connected to the recovery pipeline. The electrodialysis equipment is used to remove inorganic salt ions, such as ammonium sulfate, from the fermentation broth, and the precipitated material is returned to the pretreatment section through the recovery pipeline.

[0016] An activated carbon filter, a plate and frame filter, an arginine crystallizer, a centrifuge, and a dryer are connected in series via pipelines; the inlet of the activated carbon filter is connected to the clear liquid outlet of the electrodialysis equipment. The activated carbon filter removes pigments and heavy metal ions from the fermentation broth; the plate and frame filter removes carbon from the fermentation broth; the arginine crystallizer induces high-purity crystallization from the fermentation broth; the centrifuge separates the high-purity crystals from the fermentation broth; and the dryer dries the high-purity crystals.

[0017] Preferred technical solutions: The microfiltration membrane has a pore size of 50 nm, the ultrafiltration membrane has a molecular weight cutoff of 6000-8000 Da, and the nanofiltration membrane has a molecular weight cutoff of 800-1000 Da. A three-stage progressive filtration system is constructed to achieve stepwise removal from cell debris to small molecule impurities, improving filtration efficiency and reducing membrane fouling rate.

[0018] Preferred technical solutions: the microfiltration membrane is a tubular ceramic membrane; the ultrafiltration membrane and nanofiltration membrane are organic spiral wound membranes, which are corrosion-resistant and easy to clean.

[0019] Preferred technical solution: The first outer cavity, the second outer cavity, and the third inner cavity are all connected to the backwash water pipe through pipes with independent control valves, which can be backwashed regularly to prevent filter membrane contamination.

[0020] Preferred technical solution: A liquid level sensor is installed inside the transfer tank, and its signal output terminal is connected to the control system of the booster pump. A pressure sensor is installed inside the third outer cavity, and its signal output terminal is connected to the control system of the booster pump; the operation of the booster pump is automatically controlled to ensure pressure balance in the third outer cavity while ensuring continuous production.

[0021] Preferred technical solution: The membrane stack of the electrodialysis equipment adopts a homogeneous ion exchange membrane to improve desalination efficiency.

[0022] Preferred technical solution: The first filter, the second filter, the transfer storage tank, the third filter, the electrodialysis equipment, the activated carbon filter, the plate and frame filter, the arginine crystallization tank, the centrifuge and the dryer are connected by pipelines, and valves and transfer pumps are installed on the pipelines to facilitate flexible control of the operation of the equipment.

[0023] Preferred technical solution: The recovery pipeline is equipped with a branch line to circulate the intercepted material back to the fermentation broth conveying pipeline.

[0024] Preferred technical solution: The arginine crystallization tank is equipped with a pH adjustment device and a temperature control module to optimize crystallization conditions; and its bottom outlet is connected to a centrifuge device via a butterfly valve.

[0025] Preferred technical solution: The dryer is a vacuum dryer to avoid the damage of arginine caused by high temperature.

[0026] Due to the application of the above technical solution, the beneficial effects of this utility model compared with the prior art are as follows:

[0027] 1) High-efficiency stepwise purification: through microfiltration (removing bacteria) → ultrafiltration (removing proteins / polysaccharides) → nanofiltration (removing small molecule organic matter), a step-by-step barrier is formed, reducing the loss rate of arginine solution and improving the filtration efficiency of fermentation broth; at the same time, the first and second filters adopt internal pressure filtration, and the third filter adopts external pressure filtration and is pressurized to increase the consistency of filtration efficiency of each filter.

[0028] 2) Microfiltration, ultrafiltration, nanofiltration and electrodialysis can effectively remove impurities from arginine fermentation broth, effectively replacing ion exchange resins. It does not require the use of large amounts of ammonia water for elution, generates less wastewater, has lower costs, and is more environmentally friendly.

[0029] 3) The three-stage filtration membranes are independently connected to backwash water pipes to perform independent periodic cleaning of the microfiltration membrane, ultrafiltration membrane and nanofiltration membrane, avoiding membrane fouling and improving their service life.

[0030] 4) The retained bacteria, macromolecular impurities, etc. are returned to the pretreatment section through the recycling pipeline to improve the utilization rate of raw materials. Attached Figure Description

[0031] To more clearly illustrate the specific embodiments of this utility model or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0032] Figure 1 This is a schematic diagram of the arginine extraction device of this utility model.

[0033] In the attached diagrams above, 1 is the first filter; 2 is the second filter; 3 is the transfer tank; 4 is the third filter; 5 is the electrodialysis equipment; 6 is the activated carbon filter; 7 is the plate and frame filter; 8 is the arginine crystallization tank; 9 is the centrifuge; 10 is the dryer; 11 is the fermentation broth conveying pipeline; 12 is the recovery pipeline; 13 is the backwash water pipe; and 14 is the booster pump. Detailed Implementation

[0034] The following specific embodiments illustrate the implementation of this utility model. Those skilled in the art can easily understand other advantages and effects of this utility model from the content disclosed in this specification.

[0035] It should be noted that the terms "first," "second," etc., in the specification, claims, and accompanying drawings of this application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be used interchangeably where appropriate for the description of embodiments of this application herein. Furthermore, the terms "comprising" and "having," and their synonyms, are intended to cover non-exclusive inclusion; for example, a process, method, system, product, or apparatus that comprises a series of steps or units is not necessarily limited to those steps or units explicitly listed, but may include other steps or units not explicitly listed or inherent to such processes, methods, products, or apparatus.

[0036] In this application, the terms "upper," "lower," "left," "right," "front," "rear," "top," "bottom," "inner," "outer," "middle," "vertical," "horizontal," "lateral," and "longitudinal" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. These terms are primarily for the purpose of better describing the present invention and its embodiments, and are not intended to limit the indicated device, element, or component to having a specific orientation, or to be constructed and operated in a specific orientation.

[0037] Furthermore, in addition to indicating direction or positional relationship, some of the aforementioned terms may also have other meanings. For example, the term "above" may also be used in some cases to indicate a certain dependency or connection relationship. Those skilled in the art can understand the specific meaning of these terms in this utility model according to the specific circumstances.

[0038] Furthermore, the terms "installation," "setting," "equipped with," "connection," "linking," "fitting," and "fitting" should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral structure; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or an internal connection between two devices, components, or parts. Similarly, "fitting" can mean completely or partially fitted. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0039] It should be noted that, unless otherwise specified, the embodiments and features described in this application can be combined with each other. This application will now be described in detail with reference to the accompanying drawings and embodiments.

[0040] Example:

[0041] like Figure 1 As shown, this utility model discloses an arginine extraction device, which includes the following detailed description of the main components of this utility model:

[0042] The first filter 1 is divided into a first inner cavity and a first outer cavity by a tubular microfiltration membrane (material: ceramic membrane, pore size: 50nm); the inlet end of the first inner cavity is connected to the fermentation broth conveying pipe 11, and the outlet end of the first inner cavity is connected to the recovery pipe 12.

[0043] The second filter 2 is divided into a second inner cavity and a second outer cavity by a spiral-wound ultrafiltration membrane (material: organic spiral-wound membrane, molecular weight cutoff: 6000-8000Da); the inlet end of the second inner cavity is connected to the outlet end of the first outer cavity, and the outlet end of the second inner cavity is connected to the recovery pipe 12.

[0044] The inlet of the transfer storage tank 3 is connected to the outlet of the second outer cavity;

[0045] The third filter 4 is divided into a third inner chamber and a third outer chamber by a spiral-wound nanofiltration membrane (material: organic spiral-wound membrane, molecular weight cutoff: 800-1000Da). The inlet end of the third outer chamber is connected to the outlet end of the transfer storage tank 3 through a booster pump 14, and the outlet end of the third outer chamber is connected to the recovery pipe 12.

[0046] The electrodialysis device 5 has its inlet end connected to the outlet end of the third inner cavity, and its sewage outlet end connected to the recovery pipe 12.

[0047] Activated carbon filter 6, plate and frame filter 7, arginine crystallizer 8, centrifuge 9 and dryer 10 are connected in series via pipelines; and the inlet end of activated carbon filter 6 is connected to the clear liquid outlet end of electrodialysis equipment 5.

[0048] refer to Figure 1 As shown, the usage method and principle of this utility model are described below:

[0049] Step 1: The arginine fermentation broth is pumped into the first inner cavity of the first filter 1 through the fermentation broth delivery pipe 11. Microfiltration is performed by internal pressure to filter out bacterial residues and large particulate impurities in the fermentation broth. The retained material is returned to the pretreatment section through the recovery pipe 12. The filtered fermentation broth enters the first outer cavity and is then transported to the second inner cavity of the second filter 2.

[0050] Step 2: In the second filter 2, the fermentation broth is ultrafiltered by internal pressure to filter out large molecular impurities in the fermentation broth, and the retained material is returned to the pretreatment section through the recovery pipeline 12; the filtered fermentation broth enters the second outer cavity and is then transported to the transfer storage tank 3.

[0051] Step 3: When the liquid level in the transfer tank 3 reaches 80%, start the booster pump 14 to pump the fermentation liquid into the third outer chamber of the third filter 4.

[0052] Step 4: In the third filter 4, the fermentation broth is subjected to nanofiltration by external pressure to filter out small organic molecules in the fermentation broth, and the retained material is returned to the pretreatment section through the recovery pipeline 12; the filtered fermentation broth enters the third inner cavity and is then transported to the electrodialysis equipment 5.

[0053] Step 5: The electrodialysis equipment 5 is in operation. The fermentation broth, after the inorganic salt ions have been removed, is transported to the activated carbon filter 6 through the clear liquid outlet. The separated material is returned to the pretreatment section through the recovery pipeline 12 through the sewage outlet.

[0054] Step 6: The fermentation broth is filtered through activated carbon filter 6 to remove pigments and heavy metal ions; then it enters plate and frame filter 7 to filter out residual activated carbon particles; then it enters arginine crystallization tank 8 to precipitate arginine crystals; the precipitated arginine crystals are sent to centrifuge device 9 to reduce the water content of the arginine crystals by centrifugation; finally, the arginine crystals are sent to dryer 10 and vacuum dried to obtain the finished arginine product.

[0055] like Figure 1 As shown, the first outer cavity, the second outer cavity, and the third inner cavity are all connected to the backwash water pipe 13 through pipes with independent control valves. During use, the membrane life is extended and the maintenance cost is reduced by triggering the pressure difference through the independent backwash system.

[0056] like Figure 1 As shown, a liquid level sensor is installed in the transfer tank 3, and its signal output terminal is connected to the control system of the booster pump 14; a pressure sensor is installed in the third outer cavity, and its signal output terminal is connected to the control system of the booster pump 14 to automatically control the operation of the booster pump, ensuring pressure balance in the third outer cavity while ensuring continuous production.

[0057] like Figure 1 As shown, the membrane stack of electrodialysis equipment 5 uses a homogeneous ion exchange membrane to improve desalination efficiency.

[0058] like Figure 1 As shown, the first filter 1, the second filter 2, the transfer storage tank 3, the third filter 4, the electrodialysis equipment 5, the activated carbon filter 6, the plate and frame filter 7, the arginine crystallization tank 8, the centrifuge device 9, and the dryer 10 are connected by pipelines. Valves and transfer pumps are installed on the pipelines to facilitate flexible control of the operation of the equipment.

[0059] like Figure 1 As shown, the recovery pipeline 12 is equipped with a branch line for circulating the retained material to the fermentation broth conveying pipeline 11.

[0060] like Figure 1 As shown, the arginine crystallization tank 8 is equipped with a pH adjustment device and a temperature control module, and its bottom outlet is connected to the centrifuge device 9 through a butterfly valve to optimize crystallization conditions.

[0061] Finally, it should be noted that the above are merely preferred embodiments of the present utility model and are not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. An arginine extraction apparatus, characterized by, The application relates to a fermentation liquid recovery system, which comprises: a first filter (1) separated into a first inner cavity and a first outer cavity by a microfiltration membrane; an inlet end of the first inner cavity is connected with a fermentation liquid conveying pipeline (11), and an outlet end of the first inner cavity is connected with a recovery pipeline (12); a second filter (2) separated into a second inner cavity and a second outer cavity by an ultrafiltration membrane; an inlet end of the second inner cavity is communicated with an outlet end of the first outer cavity, and an outlet end of the second inner cavity is connected with the recovery pipeline (12); a transfer tank (3) with an inlet communicated with an outlet end of the second outer cavity; a third filter (4) separated into a third inner cavity and a third outer cavity by a nanofiltration membrane; an inlet end of the third outer cavity is communicated with an outlet end of the transfer tank (3) through a booster pump (14), and an outlet end of the third outer cavity is connected with the recovery pipeline (12); an electrodialysis device (5) with an inlet communicated with an outlet end of the third inner cavity and a sewage outlet connected with the recovery pipeline (12); an activated carbon filter (6), a plate-and-frame filter (7), an arginine crystallization tank (8), a centrifugal device (9) and a drying machine (10) are sequentially connected through pipelines in series; and an inlet end of the activated carbon filter (6) is communicated with a clear liquid outlet end of the electrodialysis device (5).

2. The arginine extraction device of claim 1, wherein: The microfiltration membrane has a pore size of 50 nm, the ultrafiltration membrane has a molecular weight cut-off of 6000-8000 Da, and the nanofiltration membrane has a molecular weight cut-off of 800-1000 Da.

3. The arginine extraction device of claim 1, wherein: The microfiltration membrane is a tubular ceramic membrane; the ultrafiltration membrane and the nanofiltration membrane are organic roll-type membranes.

4. The arginine extraction device of claim 1, wherein: The first outer cavity, the second outer cavity and the third inner cavity are all connected with a backflushing water pipeline (13) through pipelines provided with independent control valves.

5. The arginine extraction device of claim 1, wherein: The transfer tank (3) is provided with a liquid level sensor, and a signal output end of the liquid level sensor is connected with a control system of the booster pump (14); the third outer cavity is provided with a pressure sensor, and a signal output end of the pressure sensor is connected with the control system of the booster pump (14).

6. The arginine extraction device of claim 1, wherein: The membrane stack of the electrodialysis device (5) adopts a homogeneous ion exchange membrane.

7. The arginine extraction device of claim 1, wherein: The first filter (1), the second filter (2), the transfer tank (3), the third filter (4), the electrodialysis device (5), the activated carbon filter (6), the plate-and-frame filter (7), the arginine crystallization tank (8), the centrifugal device (9) and the drying machine (10) are connected through pipelines, and valves and conveying pumps are arranged on the pipelines.

8. The arginine extraction device of claim 1, wherein: The recovery pipeline (12) is provided with a shunt branch for circulating the trapped substances to the fermentation liquid conveying pipeline (11).

9. The arginine extraction device of claim 1, wherein: The arginine crystallization tank (8) is provided with a pH adjusting device and a temperature control module, and a bottom outlet end of the arginine crystallization tank (8) is communicated with the centrifugal device (9) through a butterfly valve.

10. The arginine extraction device of claim 1, wherein: The drying machine (10) is a vacuum drying machine.

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