Device for synchronously purifying and sequencing high-throughput DNA (Deoxyribonucleic Acid)

Abstract

The utility model belongs to the technical field of gene high-throughput sequencing, and particularly discloses a high-throughput DNA (Deoxyribose Nucleic Acid) synchronous purification sequencing device. The device comprises a box body, the outer side of the box body is provided with a classification assembly, the classification assembly comprises a feeding unit and a quantification unit, the feeding unit comprises at least one feeding pipe, and classification and quantification feeding can be carried out on different types of samples through the classification assembly before a DNA solution is broken. Different types of samples can be accurately distinguished through a plurality of feeding pipes, cross contamination among the samples is avoided, the accuracy and reliability of subsequent sequencing results are ensured, quantitative feeding is achieved through cooperation of a spiral conveying blade and a motor, the amount of a DNA solution entering the box body can be accurately controlled, and the consistency and stability of experimental conditions are ensured; and the quality and repeatability of sequencing data are improved, so that classified and quantitative feeding can be realized without complicated manual operation, and the working efficiency is greatly improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of gene high-throughput sequencing, more particularly, to a device for high-throughput DNA synchronous purification sequencing. BACKGROUND

[0002] High-throughput sequencing technology, also known as "next-generation" sequencing technology, is characterized by the ability to simultaneously sequence hundreds of thousands to millions of DNA molecules and shorter read lengths. The sequencing method has become a routine experimental technique. However, the recent significant advantages of the new generation of sequencing technology, such as large-scale parallel signal and pyrosequencing, have revolutionized sequencing technology. They can simultaneously sequence millions of short sequence reads. With the increase in sequence read length, the use of 454 pyrosequencing will greatly increase the probability of identifying genes in non-model species (Hudson 2008). Due to the shorter read length, this technology can only be used for sequenced model species.

[0003] According to the search, patent No. CN213506913U discloses a DNA crushing device for high-throughput DNA sequencing, which comprises a mounting protective shell, a mounting plate is fixedly connected to the lower surface of the mounting protective shell through screws, an ultrasonic transducer is fixedly connected to the annular inner side of the mounting plate, an ultrasonic vibration terminal is fixedly connected to the output end of the ultrasonic transducer, and the ultrasonic transducer is fixedly connected to the DNA crushing chamber through the ultrasonic vibration terminal. The utility model discloses a heat absorbing ring is arranged outside the DNA crushing chamber, which effectively absorbs the heat generated by ultrasonic vibration during the crushing process, thereby improving the purity of DNA fragments to a certain extent. The circulation pump is arranged to reduce the cooling cost and improve the cooling efficiency. The air cooling tank is arranged to quickly air cool and radiate the cooling liquid, thereby ensuring the cooling circulation. The water injection tank is arranged above the DNA crushing chamber to automatically clean and improve the service life of the equipment.

[0004] During the operation of the above-mentioned patent, the DNA solution is directly added into the DNA crushing chamber, which is difficult to realize quantitative feeding, and may cause unstable sample amount for each crushing, affect the consistency of the crushing effect, and further reduce the accuracy and repeatability of the subsequent sequencing results. Moreover, it is difficult to perform specific processing on different types of samples, and it is difficult to meet the diversified experimental requirements, which may greatly hinder the experimental process, increase the experimental cost and time, and reduce the quality and efficiency of the entire sequencing work. Utility model content

[0005] In order to solve the above problems, the device for high-throughput DNA synchronous purification sequencing is provided.

[0006] The device for high-throughput DNA synchronous purification sequencing provided by the present application adopts the following technical scheme:

[0007] The device for high-throughput DNA synchronous purification sequencing comprises a box body, and a classification assembly is arranged on the outer side of the box body.

[0008] The classification assembly comprises a feeding unit and a quantitative unit, the feeding unit comprises at least one feeding pipe, and the plurality of feeding pipes are used for feeding different types of samples, and the quantitative unit comprises a spiral conveying blade.

[0009] Further, the outer wall of the box body is provided with a connecting pipe, the number of the connecting pipes corresponds to the number of the feeding pipes, and each connecting pipe is arranged in an inclined manner.

[0010] Further, the bottom of each feeding pipe is in communication with the top of the corresponding connecting pipe, the number of the spiral conveying blades corresponds to the number of the connecting pipes, and each spiral conveying blade is located in the corresponding connecting pipe.

[0011] Further, one side of each connecting pipe is provided with a motor, the output end of each motor is fixedly connected with one end of the corresponding spiral conveying blade after penetrating through one side of the corresponding connecting pipe, and the inside of each box body is provided with a plurality of leakage pipes, the number of the leakage pipes corresponds to the number of the connecting pipes, and each connecting pipe is in communication with the corresponding leakage pipe after penetrating through the outer wall of the box body.

[0012] Through the above technical scheme, different types of samples can be classified and quantitatively fed before the DNA solution is broken.

[0013] Further, the top of each feeding pipe is provided with a sealing cover, one side of each feeding pipe is provided with a rotating shaft, the outer wall of each rotating shaft is provided with a fixed block, each fixed block is connected with the corresponding rotating shaft through a resistance bearing, and each fixed block is fixedly connected with the corresponding sealing cover.

[0014] Through the above technical scheme, foreign impurities are effectively prevented from entering the feeding pipe and polluting the DNA solution.

[0015] Further, one side of the box body is provided with a water tank, one side of the water tank is connected with a water inlet pipe, and one end of the water inlet pipe extends to the inside of the box body.

[0016] Through the above technical scheme, the inside of the box body can be conveniently cleaned after use.

[0017] Further, the top of the box body is rotatably connected with a box cover, one side of each box cover close to the box body is provided with a sealing ring, and the sealing ring is made of an elastic material.

[0018] Through the above technical scheme, foreign impurities are effectively prevented from entering the inside of the box body and polluting the sample.

[0019] Furthermore, the box body has an internal cavity, inside which are placed a first sound-absorbing cotton and a second sound-absorbing cotton, and the inner bottom wall of the box body has a discharge port.

[0020] The above technical solutions can effectively absorb and reduce noise.

[0021] In summary, this application includes at least one of the following beneficial technical effects:

[0022] (1) This utility model can classify and quantitatively feed different types of samples before DNA solution is broken by a classification component. Multiple feed tubes can accurately distinguish different types of samples, avoid cross-contamination between samples, and ensure the accuracy and reliability of subsequent sequencing results. The spiral conveyor blades and motor realize quantitative feeding, which can accurately control the amount of DNA solution entering the box, ensure the consistency and stability of experimental conditions, and improve the quality and reproducibility of sequencing data. In this way, classification and quantitative feeding can be achieved without complicated manual operation, which greatly improves work efficiency.

[0023] (2) This utility model can seal the feed tube when it is not in use by means of a sealing cap, which can effectively prevent external impurities from entering the feed tube and contaminating the DNA solution, thus ensuring the purity of the sample. At the same time, the setting of the resistance bearing can keep the sealing cap in a stable position after it is opened, avoiding random shaking that may affect the operation, thus providing a reliable guarantee for the smooth progress of high-throughput DNA synchronous purification and sequencing.

[0024] (3) The present invention facilitates the introduction of cleaning water into the box through the setting of water tank and water inlet pipe, which makes it easy to clean the inside of the box after use, maintain the cleanliness of the device, and provide a good environment for subsequent experimental operations. In addition, the elastic sealing ring on the box cover can fit tightly against the box when the box cover is closed, effectively preventing external impurities from entering the box and contaminating the sample, thereby improving the accuracy and reliability of the experimental results. Attached Figure Description

[0025] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0026] Figure 2 This is a top view of the overall structure of this utility model;

[0027] Figure 3 This is a cross-sectional view of the internal structure of the box of this utility model;

[0028] Figure 4 This is a schematic diagram of the internal structure of the box of this utility model;

[0029] Figure 5 For the present utility model Figure 2 Enlarged view of the structure at point A;

[0030] Figure 6 For the present utility model Figure 3 Enlarged view of the structure at point B.

[0031] Explanation of reference numerals in the attached drawings: 1. Box body; 2. Connecting pipe; 3. Feed pipe; 4. Motor; 5. Screw conveyor blade; 6. Discharge pipe; 7. Sealing cover; 8. Rotating shaft; 9. Fixing block; 10. Box cover; 11. Sealing ring; 12. Water tank; 13. Water inlet pipe; 14. Cavity; 15. First sound-absorbing cotton; 16. Second sound-absorbing cotton; 17. Discharge port. Detailed Implementation

[0032] The technical solutions in the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of this application without creative effort are within the scope of protection of this application.

[0033] Reference Figures 1-6 A high-throughput DNA simultaneous purification and sequencing device, including a housing 1, with a classification component on the outside of the housing 1;

[0034] The classification component includes a feeding unit and a quantitative unit. The feeding unit includes a feeding tube 3, and the number of feeding tubes 3 is set to at least one. Multiple feeding tubes 3 are used to feed different types of samples. The quantitative unit includes a spiral conveying blade 5, which is used to quantitatively feed the solution.

[0035] Reference Figures 1-6 The outer wall of the housing 1 is provided with connecting pipes 2, the number of connecting pipes 2 is set in a corresponding manner to the number of feeding pipes 3, each connecting pipe 2 is set at an inclination, the bottom of each feeding pipe 3 is connected to the top of the corresponding connecting pipe 2, the number of spiral conveying blades 5 is set in a corresponding manner to the number of connecting pipes 2, each spiral conveying blade 5 is located inside the corresponding connecting pipe 2, each connecting pipe 2 is provided with a motor 4 on one side, the output end of each motor 4 passes through one side of the corresponding connecting pipe 2 and is fixedly connected to one end of the corresponding spiral conveying blade 5, each housing 1 is provided with multiple discharge pipes 6 inside, the number of discharge pipes 6 is set in a corresponding manner to the number of connecting pipes 2, each connecting pipe 2 passes through the outer wall of the housing 1 and is connected to the corresponding discharge pipe 6.

[0036] The classification component enables the DNA solution to be classified and quantitatively fed to different types of samples before cleavage. The specific operation method is as follows: First, select the corresponding feed tube 3 according to the different types of samples. Since the number of feed tubes 3 is set to at least one and multiple feed tubes 3 are used to feed different types of samples, different types of DNA solutions can be added through the corresponding feed tubes 3 respectively.

[0037] When the DNA solution enters the feed tube 3, it flows into the inclined connecting tube 2 connected to it. The motor 4 on one side of the connecting tube 2 starts, driving the spiral conveying blade 5 inside the connecting tube 2 to rotate. During the rotation, the spiral conveying blade 5 plays a role in quantitatively conveying the solution. By controlling parameters such as the speed of the motor 4 and the pitch of the spiral conveying blade 5, the amount of DNA solution entering the connecting tube 2 can be precisely controlled.

[0038] Next, the quantitatively delivered DNA solution enters the corresponding leakage tube 6 inside the box 1 through the connecting tube 2. The leakage tube 6 then delivers different types of quantitatively delivered DNA solutions to the box 1, thereby realizing the classification and quantitative feeding of different types of samples before the DNA solution is crushed.

[0039] The sorting component allows for the classification and quantitative feeding of different types of samples before DNA solution disruption. Multiple feed tubes 3 can accurately distinguish different types of samples, avoiding cross-contamination between samples and ensuring the accuracy and reliability of subsequent sequencing results. The spiral conveyor blades 5, in conjunction with the motor 4, achieve quantitative feeding, precisely controlling the amount of DNA solution entering the chamber 1, ensuring the consistency and stability of experimental conditions, and improving the quality and reproducibility of sequencing data. In this way, classified and quantitative feeding can be achieved without complicated manual operations, greatly improving work efficiency.

[0040] Reference Figure 5 Each feed pipe 3 has a sealing cap 7 at the top and a rotating shaft 8 on one side. Each rotating shaft 8 has a fixing block 9 on its outer wall. Each fixing block 9 is connected to the corresponding rotating shaft 8 through a resistance bearing. Each fixing block 9 is fixedly connected to the corresponding sealing cap 7.

[0041] The sealing cap 7 can seal the feed tube 3 when it is not in use, effectively preventing external impurities from entering the feed tube 3 and contaminating the DNA solution, thus ensuring the purity of the sample. The connection between the rotating shaft 8 and the fixing block 9 makes the opening and closing of the sealing cap 7 more convenient, facilitating the addition of different types of samples. At the same time, the resistance bearing setting ensures that the sealing cap 7 remains in a stable position after being opened, avoiding random shaking that may affect the operation, thus providing a reliable guarantee for the smooth progress of high-throughput DNA simultaneous purification and sequencing.

[0042] ReferenceFigure 4 A water tank 12 is provided on one side of the tank body 1. A water inlet pipe 13 is connected to one side of the water tank 12. One end of the water inlet pipe 13 extends through into the interior of the tank body 1. A lid 10 is rotatably connected to the top of the tank body 1. Each lid 10 is provided with a sealing ring 11 on the side near the tank body 1. The sealing ring 11 is made of elastic material.

[0043] The water tank 12 and the water inlet pipe 13 facilitate the introduction of cleaning water into the chamber 1, making it easy to clean the inside of the chamber 1 after use, maintaining the cleanliness of the device, and providing a good environment for subsequent experimental operations. Furthermore, the elastic sealing ring 11 on the lid 10 can tightly fit the chamber 1 when the lid 10 is closed, effectively preventing external impurities from entering the chamber 1 and contaminating the sample. At the same time, it can also prevent liquid or gas leakage from the chamber 1, ensuring that the experiment is carried out in a relatively closed environment, thereby improving the accuracy and reliability of the experimental results.

[0044] Reference Figure 3 The box body 1 has an internal cavity 14, and the cavity 14 is provided with a first sound-absorbing cotton 15 and a second sound-absorbing cotton 16. The inner bottom wall of the box body 1 has a discharge port 17.

[0045] The first sound-absorbing cotton 15 and the second sound-absorbing cotton 16 can effectively absorb and reduce noise, providing a relatively quiet working environment for experimental personnel, reducing the harm of noise to the human body and its impact on work mood.

[0046] Working principle: First, select the corresponding feed tube 3 according to the different types of samples. Since the number of feed tubes 3 is set to at least one and multiple feed tubes 3 are used to feed different types of samples, different types of DNA solutions can be added through the corresponding feed tubes 3 respectively.

[0047] When the DNA solution enters the feed tube 3, it flows into the inclined connecting tube 2 connected to it. The motor 4 on one side of the connecting tube 2 starts, driving the spiral conveying blade 5 inside the connecting tube 2 to rotate. During the rotation, the spiral conveying blade 5 plays a role in quantitatively conveying the solution. By controlling parameters such as the speed of the motor 4 and the pitch of the spiral conveying blade 5, the amount of DNA solution entering the connecting tube 2 can be precisely controlled.

[0048] Next, the quantitatively delivered DNA solution enters the corresponding leakage tube 6 inside the box 1 through the connecting tube 2. The leakage tube 6 then delivers different types of quantitatively delivered DNA solutions to the box 1, thereby realizing the classification and quantitative feeding of different types of samples before the DNA solution is crushed.

[0049] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A device for high-throughput simultaneous purification sequencing of DNA comprising a box (1), characterized in that, The outer side of the box (1) is provided with a classification assembly; The classification assembly comprises a feeding unit and a quantitative unit, the feeding unit comprises a feeding pipe (3), the number of the feeding pipe (3) is set to at least one, and a plurality of the feeding pipes (3) are used for feeding different types of samples, and the quantitative unit comprises a spiral conveying blade (5) used for quantitatively feeding the solution.

2. The device for high-throughput DNA simultaneous purification sequencing according to claim 1, characterized in that: The outer wall of the box (1) is provided with a connecting pipe (2), the number of the connecting pipe (2) is correspondingly arranged with the number of the feeding pipe (3), and each connecting pipe (2) is inclined.

3. The device for high-throughput DNA purification sequencing in sync according to claim 2, characterized in that: The bottom of each feeding pipe (3) is in communication with the top of the corresponding connecting pipe (2), and the number of the spiral conveying blade (5) is correspondingly arranged with the number of the connecting pipe (2), and each spiral conveying blade (5) is located in the corresponding connecting pipe (2).

4. The device for high-throughput DNA purification sequencing in sync according to claim 3, characterized in that: One side of each connecting pipe (2) is provided with a motor (4), and the output end of each motor (4) is fixedly connected with one end of the corresponding spiral conveying blade (5) after penetrating through one side of the corresponding connecting pipe (2). Each box (1) is provided with a plurality of leakage pipes (6), the number of the leakage pipe (6) is correspondingly arranged with the number of the connecting pipe (2), and each connecting pipe (2) penetrates through the outer wall of the box (1) and is in communication with the corresponding leakage pipe (6).

5. The device for high-throughput DNA purification sequencing in sync according to claim 1, characterized in that: The top of each feeding pipe (3) is provided with a sealing cover (7), one side of each feeding pipe (3) is provided with a rotating shaft (8), the outer wall of each rotating shaft (8) is provided with a fixed block (9), each fixed block (9) is connected with the corresponding rotating shaft (8) through a resistance bearing, and each fixed block (9) is fixedly connected with the corresponding sealing cover (7).

6. The device for high-throughput DNA purification sequencing in sync of claim 1, wherein: One side of the box (1) is provided with a water tank (12), one side of the water tank (12) is connected with a water inlet pipe (13), and one end of the water inlet pipe (13) extends into the box (1).

7. The device for high-throughput DNA purification sequencing in sync of claim 1, wherein: The top of the box (1) is rotatably connected with a box cover (10), one side of each box cover (10) close to the box (1) is provided with a sealing ring (11), and the sealing ring (11) is made of elastic material.

8. The device for high-throughput DNA purification sequencing in sync of claim 1, wherein: The inside of the box (1) is provided with a cavity (14), the inside of the cavity (14) is provided with a first sound-absorbing cotton (15) and a second sound-absorbing cotton (16), and the inner bottom wall of the box (1) is provided with a discharge port (17).

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