Cell freezing storage device

Abstract

The utility model provides a cell freezing storage device. The cell freezing storage device comprises a storage tank and a tank cover, an upper cover plate is arranged at the upper end in the storage tank; a supporting column is rotationally arranged in the middle of the inner bottom of the storage tank, multiple sets of test tube fixing pieces are evenly arranged on the outer side wall of the supporting column in the circumferential direction, and each set of test tube fixing piece comprises an upper fixing ring fixed to the outer wall of the supporting column and a lower fixing support slidably connected with the outer wall of the supporting column; pull rings are evenly arranged on the surface of the supporting column, the lower end of each pull ring is connected with a traction piece, and the traction pieces are slidably arranged in the supporting column in a penetrating mode and can drive the second supporting rods at the lower ends of the traction pieces to slide up and down. According to the utility model, an operator can conveniently take a single test tube without influencing the cryopreservation state of other test tubes; test tubes can be taken and placed on the upper cover plate, cells are convenient to take and place, and an operator does not directly contact a cell storage area, so that the operator is effectively prevented from being frostbitten; when an operator takes and places the cells, the operator does not directly contact with the cell storage area, so that bacteria can be prevented from being generated in the cell storage area.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell storage technical field, concretely relates to a cell cryopreservation device. BACKGROUND

[0002] Stem cell is a kind of pluripotent cell with self-replication capacity.Under certain conditions, it can differentiate into various functional cells.According to the development stage of stem cell, it is divided into embryonic stem cell and adult stem cell.According to the development potential of stem cell, it is divided into three categories: totipotent stem cell, multipotent stem cell and unipotent stem cell (special stem cell).Stem cell is a kind of cell that is not fully differentiated and immature, has the potential function of regenerating various tissues and organs and human body, and is called "universal cell" in medical field.

[0003] When stem cells are scientifically researched, the sampled stem cells need to be cryopreserved, currently, inert gas liquid nitrogen is usually used to cool and refrigerate cells, but the current cell storage device still has the following defects when in use:

[0004] (1) whether putting in or taking out cell is inconvenient, when opening container, liquid nitrogen instantaneously gasifies and is easy to freeze the operator, has certain limitation;

[0005] (2) when needing to sample and check frozen cell, only single test tube is needed to be taken out, and the existing storage device will affect the frozen state of cells in other test tubes when taking out single test tube;

[0006] (3) operator is easy to make cell preservation device produce bacteria when putting in cell or taking out cell.

[0007] In summary, currently, it is urgent to design a cell cryopreservation device overcoming the above problems. Utility model content

[0008] The utility model aims at providing a cell cryopreservation device overcoming the above problems.

[0009] In order to realize the above purpose, the utility model adopts the technical scheme of a cell cryopreservation device, comprising:

[0010] Storage tank and tank cover arranged on the upper end of the storage tank;

[0011] The upper end of the inside of the storage tank is provided with an upper cover plate;

[0012] A plurality of groups of test tube fixing members are uniformly arranged on the outer wall of the support column in a circumferential direction, each group of the test tube fixing members comprises an upper fixing ring fixed to the outer wall of the support column by a first supporting rod and a lower fixing support slidably connected to the outer wall of the support column by a second supporting rod, and the upper fixing ring and the lower fixing support are arranged in a corresponding manner.

[0013] A plurality of pull rings are uniformly arranged on the upper surface of the support column and equal in number to the test tube fixing members, the lower end of each pull ring is connected to a pulling member, the pulling member is slidably arranged in the support column and can drive the second supporting rod at the lower end thereof to slide up and down, the upper end of the support column penetrates through the upper cover plate, a through hole corresponding to any of the upper fixing rings is formed in one side of the upper surface of the upper cover plate, and a sealable cover is arranged on the through hole.

[0014] The technical problems can be further solved by the following technical measures.

[0015] Further, a plurality of vertical sliding grooves are uniformly formed in the outer wall of the lower end of the support column in a circumferential direction, the vertical sliding grooves are in one-to-one correspondence with the second supporting rods, a sliding block is slidably arranged in the vertical sliding groove, the end of the second supporting rod away from the lower fixing support is fixedly connected to the corresponding sliding block, and the lower end of the pulling member is fixedly connected to the upper end of the corresponding sliding block.

[0016] Further, the pulling member is a pull rope.

[0017] Further, a reference line is arranged on the side of the through hole close to the support column, and a plurality of indication lines corresponding to the pull rings are arranged on the upper surface and the side wall of the support column.

[0018] Further, the inner walls of the upper fixing ring and the lower fixing support are provided with protective soft pads.

[0019] Further, the upper cover plate is detachably connected to the storage tank.

[0020] Further, an annular groove is formed in the inner wall of the upper end of the storage tank, the side wall of the annular groove is provided with an internal thread, and the outer side wall of the upper cover plate is provided with an external thread matched with the internal thread.

[0021] A through hole matched with the support column is formed in the middle of the upper cover plate, and an annular gasket is arranged on the inner wall of the through hole.

[0022] Further, a support is fixedly arranged on the inner bottom wall of the storage tank, and the lower end of the support column is rotatably connected to the upper end of the support by a bearing.

[0023] The outer side wall of the support is fixed with an annular hole plate at the upper end, which divides the storage tank into a liquid nitrogen input area and a cell storage area.

[0024] Further, the liquid nitrogen input pipe and the liquid nitrogen discharge pipe are arranged on the side wall of the liquid nitrogen input area, and the electromagnetic valve is arranged on the liquid nitrogen input pipe and the liquid nitrogen discharge pipe respectively.

[0025] Further, the temperature sensor is arranged on the side wall of the cell storage area, and the controller is arranged on the outer wall of the storage tank and electrically connected with the temperature sensor, the pressure gauge and the electromagnetic valve.

[0026] Compared with the prior art, the cell freezing storage device has the following beneficial effects:

[0027] 1. The cell freezing storage device is characterized in that the upper cover plate is arranged at the inner upper end of the storage tank, the support column is rotatably arranged at the bottom of the storage tank, the plurality of test tube fixing members are evenly arranged on the outer side wall of the support column in the circumferential direction, the test tube fixing members are divided into the upper fixing ring and the lower fixing support, the upper fixing ring is fixed on the support column, the lower fixing support is slidably arranged on the support column, the test tube is arranged in the upper fixing ring and the lower fixing support, the lower fixing support can be moved up and down through the pull ring and the pulling member, the upper end of the support column is arranged on the upper cover plate, the through hole is arranged on the upper cover plate, the lower fixing support can be moved up by pulling the pull ring, the test tube can be moved out of the through hole to the upper cover plate, the single test tube can be conveniently taken by the operator, and the freezing state of the other test tubes is not affected.

[0028] 2. The cell freezing storage device is characterized in that the upper cover plate is detachably connected in the storage tank, the upper cover plate can be opened when needed, the cells in the storage tank can be integrally taken, placed, viewed or repaired, and the practicability of the storage device is effectively improved.

[0029] 3. The cell freezing storage device, through the liquid nitrogen input pipe and the liquid nitrogen discharge pipe arranged on the side wall of the liquid nitrogen input area, and through the electromagnetic valve and the pressure gauge, the controller is arranged on the outer wall of the storage tank, the opening and closing of the electromagnetic valve on each pipeline can be controlled through the controller, so that the purpose of inputting liquid nitrogen or discharging liquid nitrogen is realized, and the pressure value when the liquid nitrogen is input can be monitored through the pressure gauge, the safety in the use process of the liquid nitrogen is improved; the temperature sensor is arranged in the cell storage area, which can be used for monitoring the temperature value of the cell storage area, so that the temperature value is displayed on the display screen through the controller, so that the operating personnel can view in real time, and the freezing temperature in the tank is convenient to regulate and control. BRIEF DESCRIPTION OF DRAWINGS

[0030] Figure 1 is the overall structure schematic diagram of the cell freezing storage device provided by the utility model;

[0031] Figure 2 is the overall structure schematic diagram of the cell freezing storage device after the upper cover plate is removed provided by the utility model;

[0032] Figure 3 is the cross section structure schematic diagram of the cell freezing storage device provided by the utility model;

[0033] Figure 4 is Figure 1 the enlarged schematic diagram of A in figure

[0034] BRIEF DESCRIPTION OF DRAWINGS

[0035] 1, storage tank;11, annular groove;111, internal thread;12, support;13, bearing;14, annular orifice plate;15, liquid nitrogen input pipe;16, liquid nitrogen discharge pipe;17, electromagnetic valve;18, pressure gauge;

[0036] 2, tank cover;

[0037] 3, upper cover plate;31, through hole;32, sealing cover;34, external thread;35, perforation;36, annular gasket;

[0038] 4, support column;41, vertical sliding groove;42, sliding block;

[0039] 5, test tube fixing part;51, first support rod;52, upper fixing ring;53, second support rod;54, lower fixed support;55, pulling part;56, pull ring;57, protective soft pad;

[0040] 6, controller;61, temperature sensor;

[0041] 7, reference line;71, indication line. DETAILED DESCRIPTION

[0042] In order to make the purpose, technical scheme and advantages of the utility model more clearly, the following will be further described in detail in combination with the drawings and examples.

[0043] Please refer to Figures 1 to 4 A cell cryogenic storage device, comprising:

[0044] The storage tank 1 and the tank cover 2 arranged on the upper end of the storage tank 1, one end of the tank cover 2 is hinged with the storage tank 1, and the other end is connected with the storage tank 1 through the buckle. The lower surface edge of the tank cover 2 and the upper surface edge of the storage tank 1 are provided with sealing rings, which facilitate further sealing of the inside of the storage tank 1;

[0045] The upper end of the inside of the storage tank 1 is provided with an upper cover plate 3, that is, the upper cover plate 3 covers the tank opening of the storage tank 1;

[0046] The inner bottom of the storage tank 1 is rotatably provided with a support column 4, and the outer wall of the support column 4 is uniformly provided with a plurality of groups of test tube fixing pieces 5 along the circumference. Each group of test tube fixing pieces 5 comprises an upper fixing ring 52 fixed on the outer wall of the support column 4 through a first supporting rod 51 and a lower fixing support 54 slidably connected with the outer wall of the support column 4 through a second supporting rod 53, and the upper fixing ring 52 and the lower fixing support 54 are arranged in correspondence with each other. Each group of upper fixing ring 52 and lower fixing support 54 can be used to place the same test tube, and the second supporting rod 53 can drive the lower fixing support 54 to move up and down, so that a single test tube can move up and down;

[0047] The upper surface of the support column 4 is uniformly provided with a plurality of pull rings 56 equal to the number of the test tube fixing pieces 5, and the lower end of each pull ring 56 is connected with a pulling piece 55, the pulling piece 55 is slidably arranged in the support column 4 and can drive the second supporting rod 53 at the lower end to slide up and down, the upper end of the support column 4 penetrates through the upper cover plate 3, and the upper surface of the upper cover plate 3 is further provided with a through hole 31 corresponding to any upper fixing ring 52, and the through hole 31 is provided with a sealable cover 32.

[0048] In the utility model, the support column 4 can be rotated on the upper cover plate 3, the required test tube is aligned with the through hole 31, then the pull ring 56 is pulled upwards, the pulling member 55 is driven by the pull ring 56 to move upwards, the second supporting rod 53 and the lower fixed support 54 are further moved upwards, when the upper end of the test tube is located in the through hole 31, the sealing cover 32 is opened by the other hand, the upper end of the test tube is gradually moved upwards out of the through hole 31, the test tube can be taken, the freezing state of the cells in other test tubes is not affected, the test tube can be taken and placed on the upper cover plate 3, the cell is convenient to take and place, the operator does not directly contact the cell storage area, freezing of the operator is effectively avoided, the operator does not directly contact the cell storage area when taking and placing the cell, bacteria in the cell storage area can be avoided.

[0049] Preferably, the outer side wall lower end of the support column 4 is uniformly provided with a plurality of vertical sliding grooves 41 in the circumference, the plurality of vertical sliding grooves 41 are respectively one-to-one corresponding to the plurality of second supporting rods 53, the sliding block 42 is slidably arranged in the vertical sliding groove 41, the end of the second supporting rod 53 away from the lower fixed support 54 is fixedly connected with the corresponding sliding block 42, and the lower end of the pulling member 55 is fixedly connected with the upper end of the corresponding sliding block 42.

[0050] Preferably, the pulling member 55 is a pull rope.

[0051] In order to align the test tube with the through hole 31, the through hole 31 is provided with a reference line 7 on the side close to the support column 4, the reference line 7 is located on the horizontal line connecting the center of the through hole 31 and the center axis of the support column 4, and the upper surface and the side wall of the support column 4 are respectively provided with a plurality of indication lines 71 corresponding to the pull ring 56. The indication line 71 on the upper surface of the support column 4 is a radial line from the center of the support column 4, when the test tube needs to be taken, the support column 4 is rotated, the indication line 71 corresponding to the upper end of the test tube fixing member where the test tube is located is aligned with the reference line 7, and then the operation of removing the test tube is performed.

[0052] Preferably, the inner walls of the upper fixed ring 52 and the lower fixed support 54 are provided with protective soft pads 57, so that the test tube can be prevented from colliding with the hard components.

[0053] Preferably, the upper cover plate 3 and the storage tank 1 are detachably connected. The upper cover plate 3 can be opened when needed, the cells in the storage tank are conveniently taken and placed as a whole and viewed, or the components in the storage tank are conveniently repaired, and the practicability of the storage device is effectively improved.

[0054] Preferably, an annular groove 11 is formed in the inner wall of the upper end of the storage tank 1, and the side wall of the annular groove 11 is provided with an internal thread 111, and the outer side wall of the upper cover plate 3 is provided with an external thread 34 matched with the internal thread 111.

[0055] An annular gasket 36 is arranged on the inner wall of the through hole 35 in the middle of the upper cover plate 3, which is matched with the support column 4, and can play a better sealing effect, and at the same time increase the friction when the support column 4 rotates, and increase the stability.

[0056] Preferably, a support 12 is fixedly arranged on the inner bottom wall of the storage tank 1, and the lower end of the support column 4 is rotatably connected to the upper end of the support 12 through a bearing 13.

[0057] An annular hole plate 14 is fixedly arranged on the outer side wall of the upper end of the support 12, and the annular hole plate 14 divides the storage tank 1 into a liquid nitrogen input area and a cell storage area.

[0058] Preferably, a liquid nitrogen input pipe 15 and a liquid nitrogen discharge pipe 16 are arranged on the side wall of the liquid nitrogen input area, and an electromagnetic valve 17 is arranged on each of the liquid nitrogen input pipe 15 and the liquid nitrogen discharge pipe 16, and a pressure gauge 18 is further arranged on the liquid nitrogen input pipe 15. The liquid nitrogen input pipe 15 and the liquid nitrogen discharge pipe 16 are respectively connected to an external liquid nitrogen storage device and a recovery device.

[0059] Preferably, a temperature sensor 61 is further arranged on the side wall of the cell storage area, and a controller 6 is further arranged on the outer wall of the storage tank 1, and the controller 6 is electrically connected to the temperature sensor 61, the pressure gauge 18 and the electromagnetic valve 17. The controller 6 can control the opening and closing of the electromagnetic valve 17 on each pipe, so as to realize the purpose of inputting or discharging liquid nitrogen, and the pressure value during the input of liquid nitrogen can be monitored through the pressure gauge 18, so as to improve the safety during the use of liquid nitrogen; the temperature sensor 61 arranged in the cell storage area can be used for monitoring the temperature value of the cell storage area, so that the temperature value is displayed on a display screen through the controller 6, so that the operating personnel can view in real time, and the freezing temperature in the tank can be regulated and controlled.

[0060] The above describes the utility model in combination with the embodiments, but the utility model is not limited to the above-mentioned embodiments, and various changes can be made within the knowledge range possessed by those skilled in the art without departing from the purpose of the utility model.

Claims

1. A cell cryopreservation device, comprising a storage tank (1) and a tank cover (2) arranged at the upper end of the storage tank (1), characterized in that: an upper cover plate (3) is arranged at the upper end of the inner part of the storage tank (1); a support column (4) is rotatably arranged at the middle of the inner bottom of the storage tank (1), and a plurality of groups of test tube fixing members (5) are uniformly arranged on the outer wall of the support column (4) in the circumferential direction, each group of the test tube fixing members (5) comprising an upper fixing ring (52) fixed to the outer wall of the support column (4) by a first supporting rod (51) and a lower fixing support (54) slidably connected to the outer wall of the support column (4) by a second supporting rod (53), the upper fixing ring (52) and the lower fixing support (54) being arranged in a corresponding manner; a plurality of pull rings (56) are uniformly arranged on the upper surface of the support column (4) and equal in number to the test tube fixing members (5), the lower end of each pull ring (56) is connected to a pulling member (55), the pulling member (55) is slidably arranged in the support column (4) and can drive the second supporting rod (53) at the lower end thereof to slide up and down, the upper end of the support column (4) penetrates through the upper cover plate (3), and a through hole (31) corresponding to any of the upper fixing rings (52) is further arranged on one side of the upper surface of the upper cover plate (3), and a sealable cover (32) is arranged on the through hole (31).

2. A cell cryostorage device according to claim 1, wherein, a plurality of vertical sliding grooves (41) are uniformly arranged on the lower end of the outer wall of the support column (4) in the circumferential direction, and each of the plurality of vertical sliding grooves (41) corresponds to one of the plurality of second supporting rods (53), a sliding block (42) is slidably arranged in the vertical sliding groove (41), one end of the second supporting rod (53) away from the lower fixing support (54) is fixedly connected to the corresponding sliding block (42), and the lower end of the pulling member (55) is fixedly connected to the upper end of the corresponding sliding block (42).

3. A cell cryostorage device according to claim 2, wherein, The pulling member (55) is a pull rope.

4. The cell cryostorage device of claim 1, wherein, A reference line (7) is arranged on the side of the through hole (31) close to the support column (4), and a plurality of indication lines (71) corresponding to the pull rings (56) are arranged on the upper surface and the side wall of the support column (4), respectively.

5. The cell cryostorage device of claim 1, wherein, The inner walls of the upper fixing ring (52) and the lower fixing support (54) are both provided with protective soft pads (57).

6. A cell cryo-storage device according to any one of claims 1 to 5, wherein, The upper cover plate (3) is detachably connected to the storage tank (1).

7. A cell cryostorage device according to claim 6, wherein: An annular groove (11) is arranged on the inner wall of the upper end of the storage tank (1), the side wall of the annular groove (11) is provided with an inner thread (111), and the outer side wall of the upper cover plate (3) is provided with an outer thread (34) matching the inner thread (111); A through hole (35) matching the support column (4) is arranged in the middle of the upper cover plate (3), and an annular gasket (36) is arranged on the inner wall of the through hole (35).

8. A cell cryostorage device according to claim 7, wherein: A support base (12) is fixedly arranged on the inner bottom wall of the storage tank (1), and the lower end of the support column (4) is rotatably connected to the upper end of the support base (12) through a bearing (13); An annular hole plate (14) is fixedly arranged on the upper end of the outer side wall of the support base (12), and the annular hole plate (14) divides the storage tank (1) into a liquid nitrogen input area and a cell storage area.

9. A cell cryostorage device according to claim 8, wherein, The side wall of the liquid nitrogen input area is provided with a liquid nitrogen input pipe (15) and a liquid nitrogen discharge pipe (16), the liquid nitrogen input pipe (15) and the liquid nitrogen discharge pipe (16) are respectively provided with a solenoid valve (17), and the liquid nitrogen input pipe (15) is further provided with a pressure gauge (18).

10. A cell cryostorage device according to claim 9, wherein, The side wall of the cell storage area is further provided with a temperature sensor (61), and the outer wall of the storage tank (1) is further provided with a controller (6), and the controller (6) is electrically connected with the temperature sensor (61), the pressure gauge (18) and the solenoid valve (17) respectively.

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