Cell separation and extraction equipment

Abstract

The utility model provides cell separation and extraction equipment, which comprises a support (1) and an objective table (2), the objective table (2) is used for placing a bag (100), the objective table (2) is rotatably connected to the support (1), the objective table (2) comprises an objective table main body (21) and a magnet assembly (23), the magnet assembly (23) is arranged on the objective table main body (21), the surface of the objective table main body (21) is used for placing the bag (100), and the surface of the objective table main body (21) is used for placing the bag (100). A sample in the bag (100) is located in a magnetic field generated by the magnet assemblies (23), the magnet assemblies (23) are linearly arranged according to a Halbach array, the magnetic field of the working face, facing the bag (100), of the objective table body (21) is uniform, and the magnetic field intensity of the working face (212), facing the bag (100), of the objective table body (21) is larger than the magnetic field intensity of the back face, facing away from the bag (100), of the objective table body (21).

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of cell diagnosis and treatment, and particularly relates to a cell separation and extraction device. BACKGROUND

[0002] In the prior art, a cell separation and extraction device separates cells by using a planar magnetic field to attract magnetic beads. The planar magnetic field is usually achieved by using multiple magnets. However, the contact position between the magnets will generate a high magnetic field, which will cause the cells with magnetic markers to be attracted and accumulated in the area with high magnetic field strength. This causes the cells to be squeezed against each other, the magnetic beads to be hardened, and the activity of the cells to be affected. CONTENT OF THE UTILITY MODEL

[0003] The present application aims to provide a cell separation and extraction device to avoid or alleviate the problems in the background art.

[0004] The embodiment of the present application provides a cell separation and extraction device, which comprises a support and a stage. The stage is used to place a bag. The stage is connected to the support in a rotatable manner. The stage comprises a stage main body and a magnet assembly. The magnet assembly is arranged on the stage main body. The surface of the stage main body is used to place the bag, so that the sample in the bag is in the magnetic field generated by the magnet assembly. The magnet assembly is arranged in a straight line according to a Halbach array, so that the working surface of the stage main body facing the bag has a uniform magnetic field, and the magnetic field strength of the working surface of the stage main body facing the bag is greater than the magnetic field strength of the back surface of the stage main body facing away from the bag.

[0005] In at least one possible implementation, the magnet assembly comprises a first magnet, a second magnet, a third magnet and a fourth magnet. The first magnet, the second magnet, the third magnet and the fourth magnet are arranged in sequence on the surface of the stage main body in a straight line.

[0006] The magnetic induction line direction of the inside of the first magnet points to the working surface in the vertical direction. The magnetic induction line direction of the inside of the second magnet points to the first magnet. The magnetic induction line direction of the inside of the third magnet points away from the working surface in the vertical direction. The magnetic induction line direction of the inside of the fourth magnet points away from the third magnet.

[0007] In at least one possible implementation, each magnet of the magnet assembly is a strip-shaped magnet extending in the width direction of the stage. A plurality of strip-shaped magnets are arranged in the length direction of the stage.

[0008] In at least one possible implementation, the first magnet, the second magnet, the third magnet and the fourth magnet constitute a group of magnets, and the magnet assembly comprises a plurality of groups of magnets.

[0009] In at least one possible implementation, each magnet of the magnet assembly is a strip magnet extending along the width direction of the stage, and the length of the strip magnet is greater than 80% of the width of the working surface of the stage body facing the bag.

[0010] In at least one possible implementation, the stage includes a stage bottom plate, the stage body is provided with an opening below, the stage bottom plate is connected to the stage body and closes the opening, and the stage bottom plate prevents the magnet assembly from falling from the opening.

[0011] The stage further includes a stage cover plate which is connected to the stage body in a manner that can be opened and closed, and when the stage cover plate is closed, the stage cover plate covers the working surface of the stage body to prevent the bag from falling when the stage is tilted.

[0012] In at least one possible implementation, the cell separation and extraction device further includes a limiting part, the support is provided with a limiting mounting part, the limiting part is movably mounted to the limiting mounting part, and the limiting part can cooperate with the stage to block the rotation of the stage, so that the stage is kept in a horizontal or tilted state.

[0013] In at least one possible implementation, the limiting part can limit the stage to keep in a tilted state between 30 degrees and 60 degrees relative to the horizontal state.

[0014] In at least one possible implementation, the support is provided with an angle mark which is arranged circumferentially around the rotation center of the stage.

[0015] The plurality of magnets of the magnet assembly are arranged along the length direction of the stage, and the rotation axis of the stage extends along the width direction of the stage.

[0016] In at least one possible implementation, the rotation axis of the stage is located at the middle position in the length direction of the stage.

[0017] By using the above technical solution, the magnetic field formed by the magnet assembly of the Halbach array has a small difference in the magnetic field strength on the working surface, and the magnetic field lines are uniformly distributed, so that the cells labeled by the magnetic beads can be uniformly adsorbed, and the phenomenon of cell accumulation is avoided or alleviated, and it is not easy to form a hardening. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 FIG. 1 shows a structural schematic diagram of a cell separation and extraction device according to an embodiment of the present application.

[0019] Figure 2 A schematic diagram showing a partial structure of a cell separation and extraction apparatus according to an embodiment of the present application is shown.

[0020] Figure 3 A schematic diagram showing another angle of a partial structure of a cell separation and extraction apparatus according to an embodiment of the present application is shown.

[0021] Figure 4 A schematic diagram showing a structure of a stage main body of a cell separation and extraction apparatus according to an embodiment of the present application is shown.

[0022] Figure 5 A schematic diagram showing a structure of a magnet assembly of a cell separation and extraction apparatus according to an embodiment of the present application is shown.

[0023] Figure 6 A schematic diagram showing a structure of a magnet assembly of a cell separation and extraction apparatus according to an embodiment of the present application is shown.

[0024] Figure 7 A schematic diagram showing a magnetic field strength of a magnet assembly of a cell separation and extraction apparatus according to an embodiment of the present application is shown.

[0025] BRIEF DESCRIPTION OF DRAWINGS

[0026] 1 bracket 11 limit installation part 12 angle mark

[0027] 2 stage 21 stage main body 211 accommodation groove 212 working surface

[0028] 22 stage cover plate

[0029] 23 magnet assembly 231 first magnet 232 second magnet 233 third magnet 234 fourth magnet

[0030] 24 stage bottom plate

[0031] 3 limit part

[0032] 4 hanging rack

[0033] 5 pipe magnetic attraction part

[0034] 100 bag

[0035] X length direction Y width direction C circumferential direction DETAILED DESCRIPTION

[0036] In order to illustrate the above objects, features and advantages of the application more clearly, the detailed description of the specific embodiments of the application is given in this section in conjunction with the accompanying drawings. In addition to the various embodiments described in this section, the application can be implemented in other different ways, and those skilled in the art can make corresponding modifications, variations and substitutions without departing from the spirit of the application, so the application is not limited to the specific embodiments disclosed in this section. The scope of protection of the application should be subject to the claims.

[0037] As shown in Figures 1 to 7 , the embodiments of the application propose a cell separation and extraction device, which comprises a support 1, a carrier 2, a limiting part 3, a hanging frame 4 and a pipeline magnetic attraction part 5, the carrier 2 is rotatably connected to the support 1, and the rotation axis of the carrier 2 can be located at the middle position in the length direction X of the carrier 2.

[0038] The carrier 2 is used for placing a bag 100, the bag 100 is used for containing a cell sample, and the bag 100 can be a sterile blood bag or a culture bag.

[0039] As shown in Figures 1 to 5 , the carrier 2 comprises a carrier main body 21, a carrier cover plate 22, a magnet assembly 23 and a carrier bottom plate 24.

[0040] The lower surface of the carrier main body 21 can be provided with a plurality of accommodating grooves 211, the accommodating grooves 211 can be rectangular and extend along the width direction Y of the carrier main body 21. The plurality of accommodating grooves 211 can be arranged along the length direction X of the carrier main body 21. The magnet assembly 23 comprises a plurality of magnets, and each accommodating groove 211 can be provided with one magnet. An opening can be provided below the carrier main body 21, the magnet assembly 23 is loaded into the accommodating grooves 211 from the opening, and the carrier bottom plate 24 can be connected to the carrier main body 21 and close the opening, the carrier bottom plate 24 covers below the magnet assembly 23, and the carrier bottom plate 24 can prevent the magnet assembly 23 from falling off from the opening.

[0041] When the cell separation and extraction device of the application is used, the bag 100 can be placed on the working surface 212 (i.e. the upper side of Figure 2 , the lower side of Figure 4 ) of the carrier main body 21.

[0042] The carrier cover plate 22 is hingedly connected to the carrier main body 21, when the carrier cover plate 22 is closed, the carrier cover plate 22 covers above the working surface 212 of the carrier main body 21, and the bag 100 is clamped between the carrier cover plate 22 and the working surface 212 to prevent the bag 100 from falling off when the carrier 2 is rotated and inclined. For example, when the carrier cover plate 22 is closed, the carrier cover plate 22 can be connected to the carrier main body 21 by buckling or locking.

[0043] The magnet assembly 23 is arranged on the stage main body 21, and the working surface 212 of the stage main body 21 is used to place the bag 100, so that the sample in the bag 100 is in the magnetic field generated by the magnet assembly 23, and the magnet assembly 23 is arranged in a straight line according to the Halbach array.

[0044] Figure 5 The view angle of the magnet assembly 23 is shown from the upper side (the working surface 212 side) of the stage main body 21, that is, Figure 2 viewed from top to bottom, Figure 4 viewed from bottom to top.

[0045] Referring to Figure 5 , the magnet assembly 23 can include a first magnet 231, a second magnet 232, a third magnet 233, and a fourth magnet 234, which are arranged in a straight line along the length direction X of the stage 2 in the accommodating groove 211 of the stage main body 21. The first magnet 231, the second magnet 232, the third magnet 233, and the fourth magnet 234 are all provided with a plurality of them, which are arranged in a cycle in sequence. In the horizontal state of the stage 2 (referring to the horizontal state in the process of adsorbing target cells, that is, the working surface 212 is upward), the magnetic induction line direction inside the first magnet 231 points to the working surface in the vertical direction (points upward), the magnetic induction line direction inside the second magnet 232 points to the first magnet 231, the magnetic induction line direction inside the third magnet 233 points away from the working surface in the vertical direction (points downward), and the magnetic induction line direction inside the fourth magnet 234 points away from the third magnet 233.

[0046] Each magnet of the magnet assembly 23 is a strip-shaped magnet extending along the width direction Y of the stage 2, and a plurality of strip-shaped magnets are arranged in the length direction X of the stage 2. The length of the strip-shaped magnet can be greater than 80% of the width of the working surface 212 of the stage main body 21 facing the bag 100 (the recessed surface of the upper side of the stage main body 21). Preferably, the length of the strip-shaped magnet can be equal to or greater than 100% of the width of the working surface 212 of the stage main body 21 facing the bag 100 (the recessed surface of the upper side of the stage main body 21).

[0047] The first magnet 231, the second magnet 232, the third magnet 233, and the fourth magnet 234 constitute a group of magnets, and the magnet assembly 23 can include a plurality of groups of magnets.

[0048] It can be understood that Figure 5 The magnets shown in the above are only to represent the magnetic field direction of the Halbach array, and do not limit the specific number and size of the magnets. In a non-limiting example, in the length direction X of the stage 2, the first magnet can be the first magnet 231, and the last magnet can be the third magnet 233.

[0049] In Figure 7 , the abscissa represents the position along the length direction X of the stage 2, in millimeter, and the ordinate represents the magnetic field intensity, in Gauss. From Figure 7 , it can be seen that in the middle part of the length direction X of the stage 2, the magnetic field intensity only fluctuates in a small range, and the overall magnetic field is uniform.

[0050] As shown in Figure 6 and Figure 7 , on the upper surface of the stage body 21, the magnetic field formed by the magnet assembly 23 has a small difference in magnetic field intensity along the length direction X of the stage 2, and the magnetic induction line is uniformly distributed. For example, the difference in magnetic field intensity is within 5%. In this way, the cells labeled by magnetic beads can be uniformly adsorbed on the plane of the stage body 21, avoiding or alleviating the phenomenon of cell accumulation, and it is not easy to form a hard board.

[0051] Moreover, the magnetic field formed by the above-mentioned magnet arrangement can enhance the magnetic field intensity of the adsorption working surface 212, so that the magnetic field intensity of the adsorption working surface 212 is larger, while the magnetic field intensity of the lower surface facing away is smaller, which is convenient for adsorbing cells labeled by magnetic beads while having less impact on the external environment, and it is not easy to adsorb other magnetic objects in the environment (such as the desktop).

[0052] As shown in Figure 1 and Figure 2 , the support 1 is provided with a limiting installation part 11, and the limiting part 3 is movably installed on the limiting installation part 11. The limiting part 3 can fix the stage 2 at a horizontal or inclined position. For example, the limiting installation part 11 can be a through hole, and the limiting part 3 can be a pin. The stage 2 can be provided with a pin hole. When the stage 2 is inclined, the limiting part 3 can be inserted into the pin hole of the stage 2, so that the stage 2 is kept in a horizontal or inclined state.

[0053] The limiting installation part 11 and the limiting part 3 can be arranged on one side of the width direction Y of the stage 2, or the limiting installation part 11 and the limiting part 3 can be arranged on both sides of the width direction Y of the stage 2 in groups.

[0054] The limiting installation part 11 can be provided with multiple or multiple groups, and the multiple or multiple groups of limiting installation parts 11 are arranged along the circumferential direction C with the rotation center of the stage 2 as the axis. The limiting part 3 can be selectively installed on part of the multiple or multiple groups of limiting installation parts 11, so that the stage 2 is kept in an inclined state at the required angle. For example, one or a group of limiting installation parts 11 are arranged every 10 degrees along the circumferential direction C with the rotation center of the stage 2 as the axis, so that the stage 2 can adjust the inclination angle in units of 10 degrees.

[0055] The limiting part 3 can be provided with two or two groups, and when the object table 2 is kept in a horizontal state, the two or two groups of limiting parts 3 and the pin hole of the object table 2 can be matched, and the two or two groups of limiting parts 3 can be located on both sides of the rotation axis of the object table 2, so that the object table 2 is easily kept stable.

[0056] The support 1 is provided with an angle mark 12, which is arranged along the circumference C with the rotation center of the object table 2 as the axis, and the angle mark 12 marks the inclination angle of the limiting installation part 11 corresponding to the object table 2. For example, the limiting installation part 11 corresponding to the horizontal position of the object table 2 is marked as 0 degree, and the limiting installation part 11 corresponding to the inclination of 30 degrees of the object table 2 relative to the horizontal position is marked as 30 degrees.

[0057] The angle mark 12 can be provided on the support 1 by printing or engraving, or can be formed on an angle sheet, and then the angle sheet is connected to the support 1.

[0058] Further, the rotation angle range of the object table 2 limited by the limiting part 3 can be between 30 degrees and 60 degrees. It can be understood that the rotation angle of the object table 2 can be the angle of rotation in the clockwise direction, or the angle of rotation in the counterclockwise direction.

[0059] The hanging bracket 4 can be provided beside the support 1, and the hanging bracket 4 is used for hanging a bag, such as a waste bag, a collection bag, etc. The waste bag can contain the cell waste liquid that is not adsorbed, and the collection bag can contain the target cells.

[0060] The pipeline magnetic attraction part 5 can be provided beside the support 1, and the bag 100 is connected with a pipeline, and the waste liquid in the bag 100 can be discharged through the pipeline. The pipeline magnetic attraction part 5 can be columnar, which can extend in the vertical direction. The pipeline magnetic attraction part 5 can include a pipeline magnet, the pipeline magnet can form a magnetic field, the internal magnetic induction line direction of the pipeline magnet can be along the radial direction (i.e. the horizontal direction) of the pipeline magnetic attraction part 5, and the pipeline can be spirally wound on the pipeline magnetic attraction part 5.

[0061] When the target cells in the bag 100 are discharged through the pipeline, and the cell culture solution and the target cells pass through the pipeline magnetic attraction part 5, the pipeline magnetic attraction part 5 can adsorb the excess magnetic beads that are not adsorbed by the magnet assembly 23 on the inner wall of the pipeline, and not discharged together with the target cells from the bag 100, so as to avoid the mixing of the magnetic beads into the target cells.

[0062] Next, how to use the cell separation and extraction equipment of the present application to separate and extract target cells is introduced.

[0063] (S1), biological magnetic beads are added in the bag 100 containing samples, and the magnetic beads are combined with the target cells by, for example, shaking the shaker, so that the target cells are marked by the magnetic beads.

[0064] (S2), the marked bag 100 is placed on the stage 2, the stage 2 is kept horizontal, the target cells marked by the magnetic beads are adsorbed by the magnetic field generated by the magnet assembly 23.

[0065] (S3), the stage 2 is tilted at an angle (for example, 30 to 60 degrees), the waste liquid is poured out, and the cells not adsorbed are removed.

[0066] (S4), the buffer solution is added, and the cells marked by the magnetic beads are washed.

[0067] (S5), steps S2 to S4 are repeated multiple times, for example, 3 times.

[0068] (S6), the cell culture solution is added to the bag 100.

[0069] (S7), the bag 100 is taken out from the stage 2 and then placed in a constant temperature environment, for example, 37 degrees Celsius, for cell culture, for example, 4 to 5 days, so that the cells develop to the stage that the magnetic beads can naturally fall off.

[0070] (S8), the bag 100 after cell culture is placed on the stage 2 again, the magnetic beads are adsorbed, then the stage 2 is tilted at an angle (for example, 30 to 60 degrees), the target cells are taken out, and the magnetic beads are left in the bag 100.

[0071] It should be understood that at least part of the aspects or features of the above-mentioned embodiments, examples or examples can be appropriately combined.

[0072] It can be understood that in the present application, when the number of components or members is not particularly limited, the number thereof can be one or more, and here, multiple refers to two or more. For the case where the number of components or members is described as a specific number, for example, two, three, four, etc. in the drawings and / or the description, the specific number is generally exemplary and not limited, and it can be understood as multiple, i.e. two or more, but this does not mean that the present application excludes the case of one.

[0073] In the present application, unless specifically stated or limited otherwise, the terms "mounting", "assembly", "assembling", "connecting", "connection", "coupling", "joining", "abutting", "communicating", "communicate", "conducting", "fixing", "fastening" and the like shall be interpreted broadly, for example, they can be direct or indirect. For example, in terms of connection, it can be fixed connection, or detachable connection, or integral; it can be mechanical connection, or electrical connection; it can be direct connection, or indirect connection via an intermediate medium; it can be internal communication of two elements, or interaction relationship between two elements, unless specifically stated or limited otherwise. For example, in terms of communication / conducting, it can be direct communication / conducting, or indirect communication / conducting via an intermediate medium. The specific meanings of the above terms in the present application can be understood by those skilled in the art according to the specific circumstances.

[0074] In the present application, unless specifically stated or limited otherwise, one member being disposed in / mounted in / located in / contained in / placed in another member, etc. can be either of the following two cases: a part or most of the one member is located in the other member; and the one member is completely contained in the other member.

[0075] Although the present application has been described in detail using the above embodiments, it is obvious to those skilled in the art that the present application is not limited to the embodiments described in the present specification. The present application can be modified and implemented as modified embodiments without departing from the spirit and scope of the present application defined by the claims. Therefore, the description in the present specification is for the purpose of illustration only, and does not have any limiting meaning on the present application.

Claims

1. A cell separation and extraction apparatus, characterized by, The support and the stage for placing the bag, the stage is rotatably connected to the support, the stage includes a stage body and a magnet assembly, the magnet assembly is arranged on the stage body, the surface of the stage body is used for placing the bag, the sample in the bag is in the magnetic field generated by the magnet assembly, the magnet assembly is arranged in a straight line according to the Halbach array, so that the magnetic field of the working surface of the stage body facing the bag is uniform, and the magnetic field intensity of the working surface of the stage body facing the bag is greater than the magnetic field intensity of the back surface of the stage body facing away from the bag.

2. The cell separation and extraction device of claim 1, wherein, The magnet assembly includes a first magnet, a second magnet, a third magnet and a fourth magnet, the first magnet, the second magnet, the third magnet and the fourth magnet are arranged in a straight line on the surface of the stage body, The magnetic induction line direction of the inside of the first magnet points to the working surface along the vertical direction, the magnetic induction line direction of the inside of the second magnet points to the first magnet, the magnetic induction line direction of the inside of the third magnet points away from the working surface along the vertical direction, and the magnetic induction line direction of the inside of the fourth magnet points away from the third magnet.

3. The cell separation and extraction device of claim 1, wherein, Each magnet of the magnet assembly is a strip-shaped magnet extending along the width direction of the stage, and a plurality of strip-shaped magnets are arranged in the length direction of the stage.

4. The cell separation and extraction device of claim 2, wherein, The first magnet, the second magnet, the third magnet and the fourth magnet constitute a group of magnets, and the magnet assembly includes a plurality of groups of magnets.

5. The cell separation and extraction device of claim 1, wherein, Each magnet of the magnet assembly is a strip-shaped magnet extending along the width direction of the stage, and the length of the strip-shaped magnet is greater than 80% of the width of the working surface of the stage body facing the bag.

6. The cell separation and extraction device of claim 1, wherein, The stage includes a stage bottom plate, an opening is arranged below the stage body, the stage bottom plate is connected to the stage body and closes the opening, and the stage bottom plate prevents the magnet assembly from falling from the opening, The stage further includes a stage cover plate, the stage cover plate is openably and closably connected to the stage body, when the stage cover plate is closed, the stage cover plate covers the working surface of the stage body, and the bag is prevented from falling when the stage is tilted.

7. The cell separation and extraction device of claim 1, wherein, The cell separation and extraction device further includes a limiting part, the support is provided with a limiting mounting part, the limiting part is movably mounted on the limiting mounting part, and the limiting part can cooperate with the stage to block the rotation of the stage, so that the stage is kept in a horizontal or inclined state.

8. The cell separation and extraction device of claim 7, wherein, The limiting part can at least limit the stage to keep in an inclined state between 30 degrees and 60 degrees relative to the horizontal state.

9. The cell separation and extraction device of claim 1, wherein, The support is provided with an angle mark, the angle mark is arranged along the circumference with the rotation center of the stage as the axis, The plurality of magnets of the magnet assembly are arranged in the length direction of the stage, and the rotation axis of the stage extends in the width direction of the stage.

10. The cell separation and extraction device of claim 1, wherein, The rotation axis of the stage is located at the middle position in the length direction of the stage.

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