Component for pre-embedding organoid

Abstract

The utility model belongs to the field of medical instruments, and particularly relates to an organ-like pre-embedding assembly which comprises a pre-embedding box and a pre-embedding groove forming device, the pre-embedding box is a box body with an opening in the top, and the pre-embedding box is used for filling pre-embedding liquid and pre-embedding the to-be-pre-embedded organ; the pre-embedding groove forming device comprises a pre-embedding groove forming part and a handheld part; the pre-embedding groove forming part is connected to the tail end of the handheld part and used for forming a pre-embedding groove in the pre-embedding liquid solidification process. Compared with the prior art, the device disclosed by the utility model overcomes the defects that in the prior art, PDO is difficult to collect completely, the position of the PDO is difficult to locate and more slices are difficult to cut after conventional embedding due to the fact that the PDO is too small in size and small in quantity. According to the scheme, the PDO can be relatively completely collected, so that the key PDO can be collected; and positioning is easy, and the problem that the number of PDO paraffin sections is small is reliably solved.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the field of medical apparatus and instruments, and particularly relates to an assembly for organoid pre-embedding. BACKGROUND

[0002] At present, after the successful subculture of organoids (PDO), morphological verification is generally required, including HE, IHC, and FISH, etc. The common basis of these verification methods is to make paraffin blocks containing PDO, and then slice after positioning.

[0003] However, in actual operation, due to the characteristics of PDO being too small in volume and too few in quantity, compared with other embedded substances, there are disadvantages such as difficulty in collecting integrity, difficulty in positioning the location of PDO, and difficulty in cutting a large number of slices. This leads to a certain degree of difficulty in identifying PDO and hinders the development of PDO.

[0004] As disclosed in CN215339166U, the embedding box and the embedding box assembly are provided with an embedding groove, and the bottom wall of the embedding groove is provided with a positioning groove and a positioning hole spaced from the positioning groove; wherein the positioning groove is a strip-shaped structure, and is used for inserting a sheet-shaped sample and keeping the sheet-shaped sample in a vertical state; the positioning hole is a blind hole, and is used for inserting a needle-shaped sample and keeping the needle-shaped sample in a vertical state. The embedding box disclosed in the technical solution does not have the ability to concentrate small samples, which leads to the dispersion of PDO when using it for PDO embedding, and makes it difficult to collect integrity, difficult to locate the position of PDO, and difficult to cut a large number of slices.

[0005] As disclosed in CN115572660A, a microcell pre-embedding tube and a pre-embedding method are disclosed, the pre-embedding tube includes a tube body and a tube cover, the tube cover includes a vertical part and a wedge-shaped part, the wedge-shaped part is an open-bottom and closed-top structure, the bottom end of the wedge-shaped part is connected with the vertical part, and the projection area of the top end of the wedge-shaped part in the vertical direction is smaller than the projection area of the bottom end of the wedge-shaped part in the vertical direction. Although the size of the pre-embedding tube can concentrate and pre-embed microcells, the process needs to be carried out in sequence, such as centrifugation, filtration, and resuspension, which is complicated and easy to introduce impurities or spillage during the process, resulting in pre-embedding failure.

[0006] Therefore, a PDO pre-processing assembly is needed to concentrate and facilitate positioning after embedding. Utility model content

[0007] The utility model discloses a purpose is to solve at least one of above -mentioned problem and provide a kind of for organoid pre-embedding assembly, to solve the shortcoming of the conventional embedding in prior art due to the volume of PDO is too small, and quantity is less, leading to difficult to collect complete, difficult to locate the position of PDO and difficult to cut more slice quantity.The present scheme can more complete collection PDO, and then key PDO is collected;And easy positioning, reliably solve the problem of less paraffin section of PDO.

[0008] The utility model discloses a purpose is realized by following technical scheme:

[0009] A kind of for organoid pre-embedding assembly, including pre-embedding box and pre-embedding groove forming device;

[0010] The pre-embedding box is the box body of top opening, and the pre-embedding box is used to fill pre-embedding liquid and carries out pre-embedding to the organoid to be pre-embedded;

[0011] The pre-embedding groove forming device includes pre-embedding groove forming part and handheld part;The pre-embedding groove forming part is connected to the terminal of handheld part, and pre-embedding groove forming part is used to form pre-embedding groove in the pre-embedding liquid solidification process;

[0012] The pre-embedding groove forming part is arranged in the pre-embedding box filled with pre-embedding liquid, and the pre-embedding groove forming part does not contact with the pre-embedding box, so that pre-embedding groove is formed in the position of pre-embedding groove forming part after pre-embedding liquid solidification, and the organoid to be pre-embedded is dispersed in the pre-embedding groove and carries out pre-embedding.

[0013] Preferably, the upper surface of the pre-embedding groove forming part is a plane, which can be flush with the surface of the pre-embedding liquid in the pre-embedding box during use, and a pre-embedding groove with a specified shape and size can be formed.

[0014] Preferably, the cross section of the pre-embedding groove forming part is triangular, which makes the formed pre-embedding groove have a lowest point, so that the PDO can be more concentrated, easy to position and more complete after embedding.

[0015] Preferably, the height of the pre-embedding groove forming part is less than the height of the pre-embedding box, and the depth of the pre-embedding groove forming part arranged in the pre-embedding box is less than or equal to the height of the pre-embedding groove forming part.

[0016] Preferably, the assembly further includes an organoid collection pen.

[0017] The organoid collection pen includes a collection segment and a handheld segment connected as one.

[0018] The surface of the collecting section is arc-shaped, and a collecting groove for collecting the organoids is arranged at the end of the collecting section. The collecting groove on the collecting pen facilitates the transfer of the PDO from the cell culture device to the pre-embedding box.

[0019] Preferably, the collecting section is arranged in a crescent shape, and the collecting groove is arranged at the bottom of the outer convex side of the collecting section.

[0020] Preferably, the hand-holding section comprises a holding part and a turning part.

[0021] One end of the turning part is connected to the collecting section, and the other end of the turning part is connected to the holding part.

[0022] The turning part is arranged in an obtuse angle.

[0023] The two-section design facilitates the user's holding during use, and can keep the user's holding hand away from the pre-embedding box and the PDO, thereby avoiding contamination. Meanwhile, the obtuse angle between the two sections can be used at a relatively comfortable holding angle.

[0024] Preferably, an anti-slip structure is arranged on the surface of the hand-holding section. The anti-slip structure can further improve the anti-slip performance of the organoid collecting pen during use, and reduce the possibility of accidental slipping.

[0025] More preferably, the anti-slip structure is an anti-slip pattern arranged along the length direction of the hand-holding section.

[0026] Preferably, the depth of the collecting groove is 0.1-0.5 mm, and the width of the collecting groove is consistent with the width of the collecting section; the width of the collecting section is 2-6 mm.

[0027] Preferably, the organoid collecting pen is made of plastic.

[0028] Preferably, the height of the pre-embedding box is 2-7 mm, the length of the pre-embedding box is 1-3 cm, and the width of the pre-embedding box is 0.5-3 cm.

[0029] The height of the pre-embedding groove forming part is less than the height of the pre-embedding box, the length of the pre-embedding groove forming part is less than the length of the pre-embedding box, and the width of the pre-embedding groove forming part is less than the length of the pre-embedding box.

[0030] The length of the hand-holding part is 1-5 cm.

[0031] Preferably, the pre-embedding box is made of stainless steel or plastic, and the pre-embedding groove forming device is made of stainless steel or plastic.

[0032] Preferably, the pre-embedding liquid is agar.

[0033] The working principle of the organoid collecting pen is as follows:

[0034] In use, first fill the pre-embedding box with pre-embedding liquid to make the pre-embedding liquid flush with the opening surface of the pre-embedding box; when the pre-embedding liquid is not completely solidified, put the pre-embedding groove forming device into the pre-embedding liquid, the upper surface of the pre-embedding groove forming part is parallel to the liquid surface, and the depth of the put-in is less than or equal to the height of the pre-embedding groove forming part; when the pre-embedding liquid is completely solidified, transfer the PDO and immediately scatter it on the inclined surface of the pre-embedding groove; finally, add the pre-embedding liquid again along the inclined surface of the pre-embedding groove until it is flush, and after the re-solidification, the formed PDO pre-embedding block is processed by a dehydration machine.

[0035] Compared with the prior art, the utility model has the following beneficial effects:

[0036] The component forms the pre-embedding groove with an inverted triangular shape in the pre-embedding liquid solidification process through the pre-embedding groove forming device, adds the pre-embedding liquid again and solidifies after the PDO is randomly scattered on the inclined surface of the pre-embedding groove, completes the pre-embedding, can realize the relative concentration and rapid positioning of the PDO in the embedding block, improves the collection efficiency of the PDO tissue, and can more completely collect the small amount of PDO.

[0037] Through the use of the component, the detection rate of the PDO in the embedded paraffin tissue block can be increased, and the verification is more convenient and fast.

[0038] The component has the advantages of simple structure, reasonable setting, low manufacturing cost, reusability and the like. BRIEF DESCRIPTION OF DRAWINGS

[0039] Figure 1 It is a structural schematic view of the component for organoid pre-embedding;

[0040] Figure 2 It is a structural schematic view of the collection section;

[0041] Figure 3 It is a side sectional structural schematic view of the collection section;

[0042] Figure 4 It is a side structural schematic view of the organoid collection pen;

[0043] Figure 5 It is a structural schematic view of the pre-embedding groove formed by using the component;

[0044] In the figure: 1-organoid collection pen; 11-collection section; 12-handheld section; 13-collection groove; 2-pre-embedding box; 21-pre-embedding groove; 3-pre-embedding groove forming device; 31-pre-embedding groove forming part; 32-handheld part. DETAILED DESCRIPTION

[0045] The utility model will be described in detail in combination with the drawings and specific embodiments.

[0046] Embodiment 1

[0047] An assembly for organoid pre-embedding, as shown in Figures 1-5 , comprises a pre-embedding box 2 and a pre-embedding groove forming device 3.

[0048] The pre-embedding box 2 is an open-top box, which is used to fill pre-embedding liquid and pre-embed organoids to be pre-embedded.

[0049] The pre-embedding groove forming device 3 comprises a pre-embedding groove forming part 31 and a hand-held part 32; the pre-embedding groove forming part 31 is connected to the end of the hand-held part 32, and the pre-embedding groove forming part 31 is used to form a pre-embedding groove 21 during the solidification of the pre-embedding liquid.

[0050] The pre-embedding groove forming part 31 is arranged inside the pre-embedding box 2 filled with pre-embedding liquid, and the pre-embedding groove forming part 31 does not contact the pre-embedding box 2, so that the pre-embedding groove 21 is formed at the position of the pre-embedding groove forming part 31 after the pre-embedding liquid solidifies, and the organoids to be pre-embedded are dispersed inside the pre-embedding groove 21 and pre-embedded.

[0051] More specifically, in this embodiment:

[0052] The assembly as a whole is composed of three parts, as shown in Figure 1 , specifically comprising: an organoid collection pen 1, a pre-embedding box 2, and a pre-embedding groove forming device 3.

[0053] The organoid collection pen 1, as shown in Figure 1 , comprises a collection section 11 at the front end and a hand-held section 12 connected to the rear end of the collection section 11. The collection section 11, as shown in Figure 2 , 3 , adopts an arc surface and is provided with a collection groove 13 (not penetrating the collection section 11) at the bottom (end) position of the collection section 11 in the width direction; specifically, the collection section 11 is designed as a crescent structure, which facilitates the non-destructive or low-loss collection of PDO samples from the cell culture equipment (such as 48-well plates, 96-well plates, etc.) inside the collection groove 13; the collection groove 13 is provided at the bottom of the outer convex side surface of the collection section 11; further, when the organoid collection pen 1 is used, the organoids can be gathered through the arc-shaped bottom edge of the end of the collection section 11, and then the PDO samples are slowly taken out along the well wall to make the organoids enter the collection groove 13. As shown in Figure 3 , the cross section of the collection section 11 of the organoid collection pen 1 presents a right-angled triangle as a whole, and in particular, the outer side surface at the lower part of the collection groove 13 (the bottom end of the collection section 11) forms a certain slope to constitute a slope structure, so as to guide the organoids and facilitate their entry into the collection groove 13. The hand-held section 12, as shown in Figure 1 , andFigure 4 As shown, it is composed of a turning part and a holding part, the turning part is connected between the collection section 11 and the holding part; the turning angle of the turning part is an obtuse angle, so that the collection section 11 and the holding part connected to both ends thereof are turned at an obtuse angle, so as to provide a more convenient and comfortable holding experience when used by the user; at least one anti-slip structure is arranged on the holding part, so as to reduce the possibility of the organ collection pen 1 falling off. Specifically, the anti-slip structure in the embodiment adopts anti-slip lines arranged along the length direction of the holding part.

[0054] The pre-embedding box 2, as shown in Figure 1 which is a box body with an open top, specifically a cube, the pre-embedding box 2 is used to accommodate a pre-embedding liquid, and cooperates with the pre-embedding groove forming device 3 to construct a pre-embedding groove 21 during the solidification process of the pre-embedding liquid, as shown in Figure 5 furthermore, the PDOs transferred into the pre-embedding groove 21 and the embedding block obtained by embedding can have the characteristics of more concentrated and complete PDOs, and are convenient for positioning the PDOs and effectively overcoming the phenomenon of less paraffin sections of the PDOs. The pre-embedding liquid mentioned in the scheme can adopt the embedding liquid used in the conventional embedding process, such as 1-2% agar.

[0055] The pre-embedding groove forming device 3, as shown in Figure 1 It can be seen that it is composed of a pre-embedding groove forming part 31 and a hand holding part 32. The pre-embedding groove forming part 31 has a triangular cross section, specifically a right-angled triangle, and the upper surface of the pre-embedding groove forming part 31 is a plane. In the embodiment, the pre-embedding groove forming part 31 is a triangular prism structure with a right-angled triangular cross section; when used, one of the vertical surfaces is used as the upper surface, and the pre-embedding groove forming part 31 is placed in or partially placed in the pre-embedding liquid in the form that the upper surface is parallel to the liquid surface of the pre-embedding liquid, so as to form a pre-embedding groove 21 surrounded by an inclined surface and three vertical surfaces after the pre-embedding liquid is solidified. The hand holding part 32 of the pre-embedding groove forming device 3 is an extension of the inclined surface of the pre-embedding groove forming part 31, so as to be held by the user away from the pre-embedding liquid when used. The pre-embedding groove forming part 31 of the pre-embedding groove forming device 3 can be a solid structure, or can be hollow to reduce the overall weight.

[0056] In the present assembly, to adapt to the operation and size of PDO, the following measures are taken: the organoid collection pen 1 is made of plastic, preferably a pen-shaped structure made of medical plastic; the depth of the collection groove 13 is 0.1-0.5mm, preferably 0.2mm; the width of the collection groove 13 is consistent with the width of the collection section 11; the width of the collection section 11 is 2-6mm, preferably 5mm; the length of the part of the handheld section 12 below the corner to the collection section 11 is 1.5-3cm, preferably 2cm; the length of the part of the handheld section 12 above the corner, including the grip, is suitable for hand holding and is not particularly limited. The pre-embedding box 2 is made of stainless steel or plastic, preferably a cubic structure made of medical stainless steel or medical plastic, with a height of 2-7mm, preferably 5mm; a length of 1-3cm, preferably 2cm; and a width of 0.5-3cm, preferably 2cm. The pre-embedding groove forming device 3 is made of stainless steel or plastic, preferably medical stainless steel or medical plastic, and the length, width, and height of the pre-embedding groove forming part 31 should match those of the pre-embedding box 2, which are slightly smaller than the dimensions of the pre-embedding box 2; the length of the handheld part 32 is 1-5cm, preferably 2cm.

[0057] When the present assembly is used:

[0058] First, pour the pre-embedding liquid into the pre-embedding box 2 so that the liquid surface is flush with the opening of the pre-embedding box 2;

[0059] When the pre-embedding liquid is not completely solidified, the user can hold the pre-embedding groove forming device 3 and insert it into the pre-embedding liquid, while trying to control the upper surface of the pre-embedding groove forming part 31 to be parallel to the liquid surface of the pre-embedding liquid, and the depth of the pre-embedding groove forming part 31 should be less than or equal to the height of the pre-embedding groove forming part 31, while also controlling the pre-embedding groove forming device 3 not to contact the pre-embedding box 2, to construct a pre-embedding groove 21 in the shape of a triangular prism similar to the pre-embedding groove forming part 31;

[0060] After the pre-embedding liquid is completely solidified, the pre-embedding groove forming device 3 is removed, and a pre-embedding groove 21 with an inclined surface is obtained to accommodate the PDO;

[0061] Subsequently, the user holds the organoid collection pen 1 and inserts the collection section 11 at the front end into the cell culture device (here, a 48-well plate is used as an example), uses the arc surface to gather the PDO samples together, and then slowly removes the PDO samples along the well wall using the side with the collection groove 13, and then pours the collected PDO samples onto the inclined surface of the pre-embedding groove 21 that has been constructed, allowing the PDO samples to randomly fall onto the inclined surface.

[0062] Finally, pre-embedding liquid is added again along the inclined surface of the pre-embedding groove 21 to fill the pre-embedding groove 21 to the original liquid level, and then the pre-embedding block containing the PDO formed by solidification can be placed into the embedding box for regular dehydration treatment and subsequent testing.

[0063] The component can improve the collection efficiency of PDO tissues, can more completely collect a small amount of PDO, and increase the detection rate of PDO in paraffin tissue blocks, so that verification is more convenient and fast.

[0064] The above description of the embodiments is for the purpose of enabling a person skilled in the art to understand and use the utility model. Those skilled in the art can obviously make various modifications to the embodiments, and apply the general principles described herein to other embodiments without creative labor. Therefore, the utility model is not limited to the above embodiments, and improvements and modifications made by those skilled in the art without departing from the scope of the utility model should be within the protection scope of the utility model.

Claims

1. An assembly for organoid pre-embedding, characterized in that, The pre-embedding box (2) and the pre-embedding groove forming device (3) are included. The pre-embedding box (2) is an open-top box body, and is used for filling a pre-embedding liquid and pre-embedding an organoid to be pre-embedded. The pre-embedding groove forming device (3) includes a pre-embedding groove forming part (31) and a hand-held part (32). The pre-embedding groove forming part (31) is arranged in the pre-embedding box (2) filled with the pre-embedding liquid, and the pre-embedding groove forming part (31) is not in contact with the pre-embedding box (2), so that the pre-embedding groove (21) is formed at the position of the pre-embedding groove forming part (31) after the pre-embedding liquid is solidified, and the organoid to be pre-embedded is dispersed in the pre-embedding groove (21) and pre-embedded.

2. An assembly for organoid pre-embedding according to claim 1, wherein, The upper surface of the pre-embedding groove forming part (31) is a plane.

3. An assembly for organoid pre-embedding according to claim 1, wherein, The cross section of the pre-embedding groove forming part (31) is a triangle.

4. An assembly for organoid pre-embedding according to claim 1, wherein, The height of the pre-embedding groove forming part (31) is less than the height of the pre-embedding box (2), and the depth of the pre-embedding groove forming part (31) arranged in the pre-embedding box (2) is less than or equal to the height of the pre-embedding groove forming part (31).

5. The assembly for organoid pre-embedding of claim 1, wherein, The assembly further includes an organoid collection pen (1). The organoid collection pen (1) includes a collection section (11) and a hand-held section (12) connected as a whole. The surface of the collection section (11) is a curved surface, and a collection groove (13) for collecting organoids is arranged at the end of the collection section (11).

6. An assembly for organoid pre-embedding according to claim 5, wherein, The collection section (11) is arranged in a crescent shape, and the collection groove (13) is arranged at the bottom of the convex side of the collection section (11).

7. An assembly for organoid pre-embedding according to claim 5, wherein, The hand-held section (12) includes a holding part and a turning part. One end of the turning part is connected to the collection section (11), and the other end of the turning part is connected to the holding part. The turning part is arranged in an obtuse angle.

8. An assembly for organoid pre-embedding according to claim 5, wherein, The surface of the hand-held section (12) is provided with an anti-slip structure.

9. An assembly for organoid pre-embedding according to claim 5, wherein, The depth of the collection groove (13) is 0.1-0.5 mm, and the width of the collection groove (13) is consistent with the width of the collection section (11); the width of the collection section (11) is 2-6 mm.

10. The assembly for organoid pre-embedding of claim 1, wherein, The height of the pre-embedding box (2) is 2-7 mm, the length of the pre-embedding box (2) is 1-3 cm, and the width of the pre-embedding box (2) is 0.5-3 cm. The height of the pre-embedding groove forming part (31) is less than the height of the pre-embedding box (2), the length of the pre-embedding groove forming part (31) is less than the length of the pre-embedding box (2), and the width of the pre-embedding groove forming part (31) is less than the length of the pre-embedding box (2). The length of the hand-held part (32) is 1-5 cm.

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