Fishery culture water DNA detection sampling device

Abstract

The utility model provides a DNA (deoxyribonucleic acid) detecting and sampling device for a fishery culture water body. The DNA detecting and sampling device comprises a sampling sleeve, a fixed sleeve and a piston rod, the sampling sleeve comprises a fixed part at the upper part, a hollow part in the middle and inside and a sampling tube placing part at the lower part, and the fixed part at the upper part of the sampling sleeve is provided with a fixed sleeve; the fixed sleeve is fixedly mounted at the upper part of the sampling sleeve, and a through threaded groove is formed in the middle of the fixed sleeve and is communicated with the interior of the sampling sleeve; the piston rod penetrates through the fixed sleeve in a threaded manner and extends to the cavity in the inner wall of the sampling sleeve. According to the utility model, the length-adjustable sampling sleeve and the fixed sleeve are matched with the graduated scale, so that accurate positioning sampling of different water layer depths is realized; the whole structure is simple and reasonable, the operation is convenient, and the high-precision sampling requirement of DNA detection of fishery culture water is particularly met.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the field of water sampling, specifically a fishery culture water body DNA detection sampling device. BACKGROUND

[0002] In the field of fishery culture and water environment monitoring, obtaining DNA samples of different water layers is of great significance for studying water microbial community structure, pathogen detection, etc. The common water sample collection devices at present are mostly simple water samplers or suction devices, which have problems such as inaccurate sampling depth control, cross contamination of different water layer samples, etc.

[0003] Traditional layered sampling equipment is often complex in structure, inconvenient to operate, and difficult to achieve immediate sealing after sampling, resulting in mixing of samples during lifting and affecting the accuracy of subsequent DNA detection. In addition, the fixing method of the existing device for the sampling tube is relatively simple, and loosening or leakage may occur during sampling, which cannot meet the sampling requirements of high-precision DNA detection. UTILITY MODEL CONTENTS

[0004] In order to solve the above technical problems, the utility model provides a fishery culture water body DNA detection sampling device to solve the problems of complex structure, inconvenient operation, and difficulty in achieving immediate sealing after sampling, resulting in mixing of samples during lifting, easy loosening or leakage, and inability to meet the sampling requirements of high-precision DNA detection.

[0005] A fishery culture water body DNA detection sampling device, comprising a sampling sleeve, a fixed sleeve and a piston rod;

[0006] The sampling sleeve comprises a fixed part, a middle part and a hollow part inside, and a sampling tube placement part at the lower part, and the upper fixed part of the sampling sleeve is provided with a fixed sleeve;

[0007] The fixed sleeve is fixedly installed on the upper part of the sampling sleeve, and a threaded groove penetrating through the middle part of the fixed sleeve is provided and communicates with the inside of the sampling sleeve;

[0008] The piston rod is threaded through the fixed sleeve and extends to the cavity of the inner wall of the sampling sleeve.

[0009] The utility model discloses a fishery culture water body DNA detection sampling device, preferably the length of the fixed sleeve and the piston rod can be selected according to the depth of the culture water body.

[0010] The utility model discloses a fishery culture water body DNA detection sampling device, preferably the fixed sleeve is provided with a scale, and the scale is used for determining the measured depth.

[0011] This utility model discloses a DNA testing and sampling device for aquaculture water bodies. Preferably, the lower inner cavity of the sampling sleeve is provided with a limiting pad, which is used to limit the placement of the sampling tube.

[0012] This utility model discloses a DNA testing and sampling device for aquaculture water bodies. Preferably, a detachable piston is installed at the end of the piston rod that extends into the sampling sleeve, and the size of the piston matches the size of the sampling tube.

[0013] This utility model discloses a DNA detection and sampling device for aquaculture water bodies. Preferably, the piston has a frustum-shaped structure and can be made of rubber or silicone.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] This utility model (1) achieves precise positioning and sampling at different water depths by using an adjustable length sampling sleeve and a fixed sleeve in conjunction with a scale design; (2) adopts a piston rod with threaded drive and a frustum-shaped piston structure, which can not only accurately control the sampling process, but also achieve reliable sealing of the sampling tube, effectively preventing cross-contamination of samples; (3) the unique limiting pad design ensures that the sampling tube is firmly fixed and avoids loosening during the sampling process; (4) the detachable piston structure is easy to replace and maintain, and adapts to the usage requirements of sampling tubes of different specifications; (5) the overall structure is simple and reasonable, and easy to operate, especially suitable for the high-precision sampling requirements of DNA detection in aquaculture waters. Attached Figure Description

[0016] Fig. 1 This is a three-dimensional structural diagram of the present invention;

[0017] Fig. 2 This is a three-dimensional structural diagram of the lower part of the sampling sleeve of this utility model;

[0018] Fig. 3 This is a three-dimensional structural diagram of the present invention viewed from below.

[0019] In the diagram: 1. Sampling sleeve; 11. Limiting pad; 2. Fixing sleeve; 21. Scale; 3. Piston rod; 31. Piston. Detailed Implementation

[0020] The embodiments of this utility model will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and should not be construed as limiting the scope of this utility model.

[0021] Example 1: As Figs. 1-3 The present invention relates to a DNA sampling device for aquaculture water, comprising a sampling sleeve 1, a fixing sleeve 2, and a piston rod 3;

[0022] The sampling sleeve 1 includes a fixed part, a middle part and a hollow part inside, the middle part and the hollow part inside can be used to place a sampling tube, a lower sampling tube placing part, and a fixed sleeve 2 is installed on the upper fixed part of the sampling sleeve 1;

[0023] The fixed sleeve 2 is fixedly installed on the upper part of the sampling sleeve 1, and a threaded groove is formed in the middle part of the fixed sleeve 2 and communicates with the inside of the sampling sleeve 1;

[0024] The piston rod 3 is screwed through the fixed sleeve 2 and extends to the cavity of the inner wall of the sampling sleeve 1, and the depth of the piston rod 3 placed in the sampling sleeve 1 can be controlled by rotating the piston rod 3.

[0025] The length of the fixed sleeve 2 and the piston rod 3 can be selected according to the depth of the aquaculture water body, and different lengths of the sampling sleeve 1 and the fixed sleeve 2 are used when sampling different depths of the aquaculture water body, so that the sampling sleeve 1 can be placed inside the aquaculture water body at different depths, thereby sampling the water at different depths of the aquaculture water body.

[0026] In example two, different from example one, a scale 21 is arranged on the fixed sleeve 2, and the scale 21 is used to determine the measured depth, and when the fixed sleeve 2 is placed in the aquaculture water body at different depths, the depth of the aquaculture water body can be understood through the scale 21.

[0027] In example three, a limiting pad 11 is arranged in the lower inner cavity of the sampling sleeve 1, and the limiting pad 11 is used to limit the placed sampling tube, and when the sampling tube is placed, the limiting pad 11 is arranged to extrude the outer wall of the sampling tube, thereby ensuring that the sampling tube can be more stably fixed on the inner cavity of the sampling sleeve 1.

[0028] In example four, different from example one, a detachable piston 31 is installed on the end of the piston rod 3 extending into the sampling sleeve 1, and the size of the piston 31 matches the size of the sampling tube. The piston 31 is a circular truncated cone structure, and the piston 31 can be made of rubber or silicone material. With the rotation adjustment of the piston rod 3, after the sampling tube is placed, the piston 31 can be connected with the head of the sampling tube, so that the piston 31 seals the sampling tube, thereby enabling sampling at any time when the sampling sleeve 1 reaches the specified depth, and sealing the sampling tube after sampling.

[0029] The use principle of the sampling device, the fishery culture water body DNA detection sampling device realizes accurate stratified sampling, the sampling sleeve 1 serves as a main frame, an adjustable sampling system is formed through the fixed sleeve pipe 2 and the piston rod 3, when the device is lowered to the target depth, the rotary piston rod 3 pushes the bottom circular table type piston 31 to move upwards, the negative pressure is formed in the sampling pipe, the water body enters the sampling pipe. After sampling is completed, the piston rod 3 is reversely rotated to make the piston 31 tightly adhere to the sampling pipe opening, a seal is formed: the rubber material of the piston 31 and the interference fit of the pipe opening form a primary seal, cross contamination of the sample does not occur in the lifting process.

[0030] The embodiments of the utility model are given for example and description, although the above has shown and described the utility model embodiment, can understand that the above embodiment is exemplary, cannot be understood as the limitation of the utility model, the ordinary skill in the art can change, modify, replace and change in the range of the utility model to the above embodiment.

Claims

1. An apparatus for detecting and sampling DNA in a fish farming water body, characterized by comprising: It comprises a sampling sleeve (1), a fixed sleeve (2) and a piston rod (3); The sampling sleeve (1) comprises a fixed part, a middle part and an empty part, and a sampling tube placing part, and the fixed part is provided with the fixed sleeve (2); The fixed sleeve (2) is fixedly installed on the upper part of the sampling sleeve (1), and a threaded groove is formed in the middle part of the fixed sleeve (2) and communicates with the inside of the sampling sleeve (1); The piston rod (3) is screwed through the fixed sleeve (2) and extends to the cavity of the inner wall of the sampling sleeve (1).

2. The apparatus according to claim 1, wherein the apparatus is characterized by: The length of the fixed sleeve (2) and the piston rod (3) can be selected according to the depth of the water body.

3. The apparatus according to claim 1, wherein the apparatus is characterized by: A scale (21) is arranged on the fixed sleeve (2), and the scale (21) is used to determine the measured depth.

4. The apparatus according to claim 3, wherein the apparatus is characterized by: A limiting pad (11) is arranged in the lower inner cavity of the sampling sleeve (1), and the limiting pad (11) is used to limit the placed sampling tube.

5. The apparatus according to claim 1, wherein the apparatus is used for detecting and sampling DNA of fishery culture water body. A detachable piston (31) is installed on the end of the piston rod (3) extending into the sampling sleeve (1), and the size of the piston (31) matches the size of the sampling tube.

6. The apparatus according to claim 5, wherein the apparatus is characterized by: The piston (31) is a circular truncated cone structure, and the piston (31) can be made of rubber or silicone.

Images