Preparation device of platelet-rich plasma

Abstract

The utility model discloses a platelet rich plasma preparation device which comprises a vacuum tube, a permeation tube and a pipeline communicated with the vacuum tube and the permeation tube, the vacuum tube is a cylindrical hollow tube, the top of the vacuum tube is provided with a pressing plug capable of being pressed, and the pressing plug is provided with an area for inserting a blood drawing needle. A first membrane assembly with the aperture of 0.2-5 microns is arranged in the vacuum tube, and the first membrane assembly comprises a first membrane layer, a coating layer arranged on the bottom surface of the first membrane layer and a nylon net arranged at the bottom of the coating layer; the permeation tube is U-shaped, an inlet and an outlet are respectively arranged at two ends of the U-shaped permeation tube, a hollow fiber membrane is arranged in the permeation tube, the hollow fiber membrane is bent to have a radian consistent with the appearance of the permeation tube, two ends of the hollow fiber membrane are respectively connected with the inlet and the outlet, and the molecular weight cut-off of the hollow fiber membrane is 2-40,000 Daltons. A waste discharge port is formed in the permeation pipe. According to the utility model, the preparation efficiency is improved, and high-concentration PRP (platelet rich plasma) can be obtained under the condition of less blood consumption.

Description

Technical Field

[0001] This utility model relates to the field of platelet-rich plasma filtration and separation technology, and in particular to a device for preparing platelet-rich plasma. Background Technology

[0002] PRP stands for Platelet-Rich Plasma. Normal human plasma contains a platelet concentration of (100–300) × 10^9 / L, while PRP has a platelet concentration 4–8 times higher. When activated, the abundant platelets in PRP release a large number of growth factors, which can promote bone healing, tendon repair, removal of necrotic tissue, reduction of inflammation and postoperative pain, and acceleration of articular cartilage repair and regeneration. It is currently widely used in dentistry, orthopedics, plastic surgery, and other fields.

[0003] Currently, most commercially available PRP extractors use centrifugation, but centrifugation has the following problems:

[0004] The platelet concentrate layer is located between the plasma layer and the red blood cell layer. The separation layer between platelets and white blood cells is relatively small, making it easy for more white blood cells and red blood cells to be mixed in when extracting PRP.

[0005] There are many methods and devices for PRP centrifugation, but the concentration ratios vary, and the concentration and quality may not meet or exceed the expected requirements. The platelet yield prepared by the PRP method is low, only 60% to 70% of the original platelet content, which means that some plasma that can be used for plasma protein separation cannot be fully utilized. In addition, its automation level is generally low, and the waiting time is long. Manual PRP extraction is subject to visual errors. The machine extraction process requires manual assistance.

[0006] Currently, some hospitals extract the first-pass PRP (platelet-rich plasma) through a low-speed centrifugation, followed by a second high-speed centrifugation to extract PRP free of white blood cells. This method reduces the concentration of white blood cells in the PRP and also avoids damage to red blood cells caused by high-speed centrifugation to some extent. However, this method is more cumbersome, and if the initial extraction is not done properly, high-speed centrifugation can damage red blood cells, affecting the quality of the PRP. Centrifugation methods, whether using a single or double extraction method, result in a significant amount of platelets being discarded in the plasma and red blood cell layers. Although high-speed centrifugation can increase the concentration rate, in actual clinical practice, the centrifuge equipment containing the blood cannot withstand prolonged high-speed centrifugation, which can easily lead to leakage and contamination. Utility Model Content

[0007] To address the aforementioned technical problems, the purpose of this invention is to provide a device for preparing platelet-rich plasma, which facilitates the purification of platelet-rich plasma concentration.

[0008] To achieve the above objectives, this utility model provides the following technical solution:

[0009] A platelet-rich plasma (PRP) preparation apparatus includes a vacuum tube, a permeation tube, and a conduit connecting the vacuum tube and the permeation tube. The vacuum tube is a cylindrical hollow tube with a pressable stopper at its top. The stopper has an area for inserting a blood-drawing needle. A first membrane assembly with a pore size of 0.2–5 μm is disposed inside the vacuum tube. The first membrane assembly includes a first membrane layer, a coating layer on the bottom surface of the first membrane layer, and a nylon mesh at the bottom of the coating layer. The permeation tube is U-shaped, with an inlet and an outlet at its two ends. A hollow fiber membrane is disposed inside the permeation tube, bent into an arc shape consistent with the shape of the permeation tube. Both ends of the hollow fiber membrane are connected to the inlet and outlet, respectively. The hollow fiber membrane has a molecular weight cutoff of 20,000–40,000 Daltons. A waste outlet is provided on the permeation tube. One end of the conduit is connected to the bottom of the vacuum tube, and the other end is connected to the inlet of the permeation tube. A latch for opening and closing the conduit is provided on the conduit.

[0010] Preferably, the first membrane assembly is located near the upper part of the vacuum tube, and the inner wall of the vacuum tube is provided with retaining teeth, on which a silicone ring is provided that contacts the bottom surface of the first membrane assembly.

[0011] Preferably, the first film layer is a short fiber spunbond nonwoven fabric with a fiber diameter of 8 to 10 μm.

[0012] Preferably, the nonwoven fabric is calendered by a single-sided calendering roller, and the polyethersulfone is dissolved in a solvent to form a casting solution, which is then applied to the smooth side of the nonwoven fabric to form the coating layer.

[0013] Preferably, the coating layer is bonded to the nonwoven fabric but not completely immersed in it.

[0014] Preferably, the nylon mesh is a 400-mesh nylon mesh.

[0015] Due to the application of the above technical solution, this utility model has the following advantages compared with the prior art:

[0016] This invention relates to a device for preparing platelet-rich plasma (PRP), comprising a vacuum tube, a permeation tube, and a conduit connecting the vacuum tube and the permeation tube. The vacuum tube has a pressable stopper at its top. Inside the vacuum tube is a first membrane assembly with a pore size of 0.2–5 μm. The first membrane assembly includes a first membrane layer, a coating layer on the bottom surface of the first membrane layer, and a nylon mesh at the bottom of the coating layer. The permeation tube is U-shaped, with an inlet and an outlet at each end of the U-shape. A hollow fiber membrane is placed inside the permeation tube, bent into an arc shape consistent with the shape of the permeation tube. Both ends of the hollow fiber membrane are connected to the inlet and outlet, respectively. The hollow fiber membrane has a molecular weight cutoff of 20,000–40,000 Daltons. Concentrated platelets are extracted through two sets of filter membranes with specific pore sizes. Compared to traditional centrifugation methods for preparing PRP, this method has a shorter operation time, less residual leukocytes and free hemoglobin, less influence from human factors, and improved preparation efficiency. It can obtain high concentrations of PRP with a smaller blood volume. Attached Figure Description

[0017] The technical solution of this utility model will be further described below with reference to the accompanying drawings:

[0018] Appendix Figure 1 This is a schematic diagram of the apparatus for preparing platelet-rich plasma according to this invention.

[0019] The components are: 1. Vacuum tube; 11. Clamping teeth; 2. Permeation tube; 21. Inlet; 22. Outlet; 23. Waste discharge port; 3. Pipe; 4. Plug; 5. First membrane module; 51. First membrane layer; 52. Coating layer; 53. Nylon mesh; 6. Hollow fiber membrane; 7. Buckle; 8. Silicone ring. Detailed Implementation

[0020] The preferred embodiments of the present invention will now be described in detail with reference to the accompanying drawings, so that the advantages and features of the present invention can be more easily understood by those skilled in the art, thereby making a clearer and more definite definition of the scope of protection of the present invention.

[0021] It should be noted that when a component is referred to as "fixed to" or "set on" another component, it can be directly on the other component or may have an intervening component present. When a component is referred to as "connected to" another component, it can be directly connected to the other component or may have an intervening component present.

[0022] Furthermore, it should be noted that the directional terms such as left, right, up, and down in the embodiments of this utility model are only relative concepts or references to the normal use of the product, and should not be considered restrictive. The implementation of this utility model will be described in detail below with reference to specific embodiments.

[0023] As attached Figure 1The diagram shows an apparatus for preparing platelet-rich plasma according to this invention, comprising a vacuum tube 1, a permeation tube 2, and a conduit 3 connecting the vacuum tube 1 and the permeation tube 2. The vacuum tube 1 is a cylindrical hollow tube with a pressable stopper 4 installed at its top. The stopper 4 has an area for inserting a blood-drawing needle. A first membrane assembly 5 with a pore size of 0.2–5 μm is installed inside the vacuum tube 1. The first membrane assembly 5 includes a first membrane layer 51, a coating layer 52 located on the bottom surface of the first membrane layer 51, and a nylon mesh 53 located at the bottom of the coating layer 52. In this embodiment, the first membrane layer 51 is a short-fiber spunbond nonwoven fabric with a fiber diameter of 8–10 μm, while red blood cells have a diameter of 7–8 μm, making them less prone to breakage during filtration due to the short fibers. The nonwoven fabric is calendered using a single-sided calendering roller. Polyethersulfone is dissolved in a solvent to form a casting solution, which is then applied to the smooth side of the nonwoven fabric to form a coating layer 52. Through surface calendering and adjustment of the casting solution concentration, the coating layer 52 only adheres to the nonwoven fabric and does not completely penetrate it. A 400-mesh nylon mesh 53 provides support on the other side of the coating layer 52. The polyethersulfone coating layer here requires greater rigidity; therefore, acidified polyethersulfone and acrylic copolymer are added to the polyethersulfone for reinforcement. The first membrane assembly 5 is located near the upper part of the vacuum tube 1. The inner wall of the vacuum tube 1 has retaining teeth 11, which are small rings formed during the injection molding of the vacuum tube 1. Each retaining tooth 11 has a silicone ring 8 that contacts the bottom surface of the first membrane assembly 5. During vacuum suction, after the surface of the first membrane layer 51 is wetted, this ring buffers the pressure difference across the vacuum tube 1, preventing premature descent of the pressure plug due to the pressure difference generated after blood is sealed from the membrane surface.

[0024] The permeation tube 2 is U-shaped, with an inlet 21 and an outlet 22 at each end. A hollow fiber membrane 6 is installed inside the permeation tube 2, bent into an arc shape consistent with the shape of the permeation tube 2. The two ends of the hollow fiber membrane 6 are connected to the inlet 21 and the outlet 22, respectively. The molecular weight cutoff of the hollow fiber membrane 6 is 20,000 to 40,000 Daltons. The nascent PRP formed after filtration by the first membrane module 5 enters the inner wall of the hollow fiber membrane 6 through the inlet 21, undergoes tangential flow separation, and flows out through the outlet 22. The permeation tube 2 has two waste outlets 23 to discharge the free hemoglobin that has been broken down due to filtration and compression.

[0025] One end of the pipe 3 is connected to the bottom of the vacuum tube 1, and the other end is connected to the inlet 21 of the permeation tube 2. A buckle 7 for switching the pipe 3 on and off is installed on the pipe 3.

[0026] In use, blood is drawn in under negative pressure and filtered through the first membrane assembly 5 under vacuum. The latch 7 is then opened, and the stopper 4 of the vacuum tube 1 is pressed, allowing the filtered blood to flow out through the inner wall of the hollow fiber membrane 6. Waste liquid that has permeated through the inner wall is discharged through the waste outlet 23. This invention uses filtration instead of centrifugation, extracting concentrated platelets through two sets of filter membranes with specific pore sizes. Compared to traditional centrifugation for preparing platelet-rich plasma, it has a shorter operation time, less residual white blood cells and free hemoglobin, and is less affected by human factors. The hollow fiber membrane filtration solves the safety problem of red blood cell rupture during blood filtration. The free hemoglobin content in the resulting PRP is comparable to that of low-speed centrifugation, far lower than that of high-speed centrifugation, and has a longer effective usage time compared to centrifugation.

[0027] This invention relates to a platelet-rich plasma (PRP) preparation device that can prepare PRP via filtration without reducing purity, saving costs, reducing cumbersome steps, and improving preparation efficiency. Through the cooperation of two membrane modules, negative pressure filtration and positive pressure positive osmosis are used to remove free hemoglobin broken down by filtration and compression. While PRP obtained by centrifugation contains more mixed plasma and has a lower concentration, the PRP prepared by this invention, although containing a small amount of platelets and red blood cells retained on the outer side of the membrane, absorbs more plasma proteins during filtration, thus achieving a high concentration of PRP with a smaller blood volume.

[0028] The above description is merely an embodiment of this utility model and does not limit the patent scope of this utility model. Any equivalent structural or procedural transformations made based on the content of this utility model specification, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this utility model.

Claims

1. A device for preparing platelet rich plasma, characterized by: The device includes a vacuum tube, a permeation tube, and a conduit connecting the vacuum tube and the permeation tube. The vacuum tube is a cylindrical hollow tube with a pressable plug at its top. The plug has an area for inserting a blood-drawing needle. A first membrane assembly with a pore size of 0.2–5 μm is disposed inside the vacuum tube. The first membrane assembly includes a first membrane layer, a coating layer on the bottom surface of the first membrane layer, and a nylon mesh at the bottom of the coating layer. The permeation tube is U-shaped, with an inlet and an outlet at each end of the U-shape. A hollow fiber membrane is disposed inside the permeation tube, bent into an arc shape consistent with the shape of the permeation tube. Both ends of the hollow fiber membrane are connected to the inlet and outlet, respectively. The hollow fiber membrane has a molecular weight cutoff of 20,000–40,000 Daltons. A waste outlet is provided on the permeation tube. One end of the conduit is connected to the bottom of the vacuum tube, and the other end is connected to the inlet of the permeation tube. A latch for opening and closing the conduit is provided on the conduit.

2. The platelet-rich plasma preparation device according to claim 1, characterized in that: The first membrane assembly is located near the upper part of the vacuum tube. The inner wall of the vacuum tube is provided with retaining teeth, and a silicone ring is provided on the retaining teeth to contact the bottom surface of the first membrane assembly.

3. The apparatus for preparing platelet-rich plasma according to claim 1, characterized in that: The first film layer is a short fiber spunbond nonwoven fabric with a fiber diameter of 8-10 μm.

4. The platelet rich plasma preparation apparatus according to claim 3, characterized in that: The nonwoven fabric is calendered by a single-sided calendering roller, and polyethersulfone is dissolved in a solvent to form a casting solution, which is then applied to the smooth side of the nonwoven fabric to form the coating layer.

5. The device for preparing platelet rich plasma according to claim 4, characterized in that: The coating layer is bonded to the nonwoven fabric but is not completely immersed in the nonwoven fabric.

6. The apparatus for preparing platelet-rich plasma according to claim 1, characterized in that: The nylon mesh is a 400-mesh nylon mesh.

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