Assembled low-temperature box applied to ultrasonic cell disruptor

Abstract

The assembled low-temperature box comprises a low-temperature box body, a placement supporting frame and an insertion plate, the upper portion of the low-temperature box body is open, the placement supporting frame is movably placed in the low-temperature box body in a matched mode, a placement frame is arranged on the placement supporting frame, and the insertion plate is inserted into the placement frame. The inserting plate is detachably clamped on the placing frame, the placing frame and the inserting plate are provided with a clamping mother groove and a clamping son block respectively, and the clamping son block is clamped into the clamping mother groove in a matched mode; and a plurality of inserting holes are formed in the inserting plate. According to the EP pipe inserting and placing frame, the inserting and placing plates can be flexibly and selectively assembled according to actual use requirements, the inserting and placing plates can be matched with inserting and placing of EP pipes with different hole diameters for use, the structures of the inserting and placing plates and the placing frame are simpler and more reasonable, assembling and using are convenient, and using is more convenient and more practical.

Description

Technical Field

[0001] This utility model relates to the design and application of cryogenic chambers, and more specifically to an assembled cryogenic chamber used in an ultrasonic cell disruptor. Background Technology

[0002] Ultrasonic cell disruptors are instruments used in routine biochemical experiments such as ChIP and WB to extract proteins. To prevent protein degradation, a cryogenic chamber must be used in conjunction with an ultrasonic cell disruptor. The cryogenic chamber is used for ice baths, water baths, or low-temperature water baths to maintain a low-temperature environment for the sample during operation, allowing the ultrasonic cell disruptor to perform protein extraction better.

[0003] Existing cryogenic chambers for ultrasonic cell disruptors, such as the Chinese patent: A cryogenic chamber for an auxiliary ultrasonic cell disruptor, authorized announcement number: CN216208035U, disclose the specific structure and usage of existing cryogenic chambers for ultrasonic cell disruptors. From the above disclosure, it can be seen that the cryogenic chamber can not only insert and place EP tubes and maintain their stability, but also realize water bath and ice bath for EP tubes, so that protein extraction can be better handled.

[0004] The existing low-temperature boxes still have the following shortcomings and disadvantages in use:

[0005] Existing cryogenic boxes have limitations in their use because the diameter of the insertion hole is fixed, making it impossible to insert EP tubes of different diameters according to actual needs.

[0006] This technical solution is based on the existing technology and further improves and perfects the structure of the existing low-temperature box. This solution can not only flexibly and selectively assemble the insertion plate according to actual usage requirements, so that the insertion plate can be matched with EP tubes of different aperture sizes, but also the structure of the insertion plate and the placement frame is simpler and more reasonable, making it easier to assemble and use, and more convenient and practical to use. Utility Model Content

[0007] This utility model discloses an assembled low-temperature box for use in an ultrasonic cell disruptor, the main purpose of which is to overcome the above-mentioned deficiencies and shortcomings of the prior art.

[0008] The technical solution adopted in this utility model is as follows:

[0009] An assembled cryogenic chamber for use in an ultrasonic cell disruptor includes a cryogenic chamber body, a placement support frame, and an insertion plate. The upper part of the cryogenic chamber body is open. The placement support frame is movably placed inside the cryogenic chamber body. A placement frame is provided on the placement support frame. The insertion plate is detachably snapped onto the placement frame. The placement frame and the insertion plate are respectively provided with a female insertion slot and a female insertion block. The female insertion block is engaged with the female insertion slot. The insertion plate has several insertion holes.

[0010] Furthermore, the placement frame is divided into several identical placement areas, and there are several insertion plates, which are placed on the placement areas in conjunction with corresponding cards.

[0011] Furthermore, the insertion holes on each of the individual insertion boards have the same diameter.

[0012] Furthermore, the insertion plate is equipped with a lifting column.

[0013] Furthermore, there are two card slot blocks, which are symmetrically arranged on both sides of the insertion plate.

[0014] Furthermore, the height of the support frame is less than the height of the cryogenic box body.

[0015] As can be seen from the above description and explanation of this utility model, compared with the prior art, the advantages of this utility model are as follows:

[0016] Advantage 1: This utility model, through the combination structure of the placement support frame and the insertion plate, not only allows for flexible adjustment of the insertion plate to meet the actual needs of use by adjusting the number of insertion holes of each diameter to satisfy the insertion requirements of EP tubes, but also utilizes the structure of the locking female groove, locking female block and lifting column to improve the stability after locking and placing, and make lifting and replacement more convenient.

[0017] Advantage 2: By setting up a low-temperature box body, a support frame, and an insertion plate, this utility model can not only achieve stable insertion and use of EP tubes, but also make operation and replacement more flexible and convenient, which is more conducive to the user's replacement operation, and has stronger practicality and convenience. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the structure of this utility model.

[0019] Figure 2 This is a side view of the structure of this utility model.

[0020] Figure 3 This is a schematic diagram of the structure of the insertion plate of this utility model.

[0021] Figure 4This is a structural schematic diagram of the support frame of this utility model.

[0022] The components include: cryogenic box body 1, placement support frame 2, placement frame 21, placement area 22, insertion plate 3, insertion hole 31, lifting column 32, insertion slot 4, and insertion sub-block 5. Detailed Implementation

[0023] The specific embodiments of this utility model will be further described and illustrated below with reference to the accompanying drawings.

[0024] like Figures 1 to 4 As shown, an assembled cryogenic chamber for use in an ultrasonic cell disruptor includes a cryogenic chamber body 1, a placement support frame 2, and insertion plates 3. The upper part of the cryogenic chamber body 1 is open. The placement support frame 2 is movably placed inside the cryogenic chamber body 1, and the height of the placement support frame 2 is less than the height of the cryogenic chamber body 1. A placement frame 21 is provided on the placement support frame 2, and the insertion plates 3 are detachably snapped onto the placement frame 21. The placement frame 21 and the insertion plates 3 are respectively provided with a female insertion slot 4 and a female insertion block 5. The female insertion block 5 is engaged with the female insertion slot 4. The placement frame 21 is divided into several identical placement areas 22. There are several insertion plates 3, which are engaged with corresponding placement areas 22. There are two female insertion blocks 5, which are symmetrically arranged on the left and right sides of the insertion plates 3.

[0025] The insertion plate 3 has several insertion holes 31, and the insertion holes 31 on each insertion plate 3 have the same diameter. Insertion plates 3 with different hole diameters can be inserted according to the actual requirements of the EP tube, making them more flexible and convenient to use. The insertion plate 3 is equipped with a lifting column 32, which allows for easy lifting of the insertion plate 3, making it even more convenient and quick to use. By setting up a combination structure of the placement support frame 2 and the insertion plate 3, the insertion plate 3 can be flexibly adjusted for placement, allowing for adjustment of the number of insertion holes 31 of various diameters to meet the insertion requirements of the EP tube. Furthermore, the structure of the insertion slot 4, the insertion sub-block 5, and the lifting column 32 provides better stability after insertion, and makes lifting and replacement more convenient.

[0026] How to use this utility model:

[0027] 1. Assembly of the low-temperature box: First, place the support frame 2 inside the low-temperature box body 1, then inject the liquid of the water bath, generally ice water or low-temperature water. Then, according to the number and diameter of the EP tubes to be inserted in the experiment, select different numbers of insertion plates 3. Then, hold the lifting column 32 of the insertion plate 3 and place the insertion plate 3 into the placement frame 21 on the support frame 2. The insertion sub-block 5 on the insertion plate 3 will cooperate to insert into the insertion slot 4 on the placement frame 21 to complete the assembly of the low-temperature box.

[0028] 2. Insertion of EP tube: Place the cell sample to be broken into the EP tube, and then insert the EP tube into the insertion hole 31 on the insertion plate 3. The EP tube achieves water bath and ice bath treatment, and the sample is uniformly surrounded in a low temperature environment.

[0029] 3. Cell disruption: Connect the main unit of the ultrasonic cell disruptor to the transducer, ensuring a stable connection. Then, according to experimental requirements, set appropriate parameters such as ultrasonic function, working time, and interval time. Start the ultrasonic cell disruptor to begin the disruption process. During disruption, carefully observe the state of the sample in the EP tube and the operation of the instrument to ensure that the disruption effect meets expectations. After disruption is complete, remove the PE tube sample and proceed with subsequent processing steps such as centrifugation and filtration.

[0030] The above are merely specific embodiments of this utility model, but the design concept of this utility model is not limited thereto. Any non-substantial improvements made to this utility model using this concept should be considered as infringing on the protection scope of this utility model.

Claims

1. An assembled cryogenic cassette for use in an ultrasonic cell disrupter, characterized by: The device includes a cryogenic chamber body, a placement support frame, and an insertion plate. The upper part of the cryogenic chamber body is open. The placement support frame is movably placed inside the cryogenic chamber body. A placement frame is provided on the placement support frame. The insertion plate is detachably snapped onto the placement frame. The placement frame and the insertion plate are respectively provided with a female insertion slot and a female insertion block. The female insertion block is engaged with the female insertion slot. The insertion plate has several insertion holes.

2. An assembled cryogenic cassette for use in an ultrasonic cell disrupter according to claim 1, wherein: The placement frame is divided into several identical placement areas, and there are several insertion plates, which are placed on the placement areas in conjunction with corresponding cards.

3. An assembled cryogenic cassette for use in an ultrasonic cell disrupter according to claim 2, wherein: The insertion holes on each of the aforementioned insertion boards have the same diameter.

4. An assembled cryogenic cassette for use in an ultrasonic cell disrupter according to claim 1, wherein: The insertion plate is equipped with a lifting column.

5. The assembled cryogenic chamber for use in an ultrasonic cell disruptor according to claim 1, characterized in that: There are two card slot blocks, which are symmetrically arranged on both sides of the insertion plate.

6. An assembled cryogenic cassette for use in an ultrasonic cell disrupter according to claim 1, wherein: The height of the support frame is less than the height of the cryogenic box body.

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