Sepsis experimental instrument

Abstract

The utility model discloses a sepsis experimental instrument, including first turntable, connecting rod, second turntable, support piece, side plate, shell, motor, power supply and rotating shaft, the rotating shaft extends out from shell one side, the rotating shaft passes through first turntable, the rotating shaft is fixedly connected with first turntable, one end of connecting rod is threaded connection with the rotating shaft, and the other end of connecting rod is threaded connection with the rotating shaft. The other end of the connecting rod is rotationally connected with the supporting piece, the connecting rod penetrates through the second rotating disc and is fixedly connected with the second rotating disc, the supporting piece is connected with the side plate, one side of the shell is fixedly connected with the side plate, the motor and the power source are arranged in the shell, an output shaft of the motor is connected with the rotating shaft, and the power source is electrically connected with the motor. According to the utility model, the test tubes can be rotated in a horizontal or vertical state according to the requirements of different rotating directions of the test tubes. The number of test tubes can be increased, and the number of cell culture and the number of cell and pathogen mixed samples in unit time can be increased.

Description

Technical Field

[0001] This utility model belongs to the field of experimental device technology, and in particular relates to an experimental instrument for sepsis. Background Technology

[0002] Sepsis is a life-threatening organ dysfunction characterized by an interplay of excessive inflammatory response and immunosuppression. Currently, the pathogenesis and pathophysiological changes of sepsis are not fully understood. Therefore, many researchers hope to elucidate the pathogenesis and post-sepsis pathophysiological changes through sepsis model studies, aiming to improve clinical understanding and explore new diagnostic and treatment strategies.

[0003] Intraperitoneal injection of lipopolysaccharide can replicate the pathogenesis of sepsis, making it the most popular method for establishing sepsis models. After establishing the sepsis model, intestinal epithelial cells are extracted, and cell culture can then be used to study the pathogenesis and post-sepsis pathophysiological changes. Therefore, cell culture devices are commonly used in sepsis research. Because a large number of ileal epithelial cells are used in the experiment, and different rotation conditions are required for different experimental steps, a suitable experimental rotation device is needed.

[0004] Patent document CN202124631U discloses a rotator for three-dimensional cell culture, which includes a base and a motor fixedly mounted on the base. The output shaft of the motor is connected to a rotating shaft horizontally mounted on the base and rotatable about its own axis. Several spaced-apart turntables are coaxially mounted on the rotating shaft and can rotate with the shaft. A ring of through holes for inserting test tubes is opened along the edge of the turntables. However, this rotator is used in a fixed posture, making it difficult to use it axially upright. Utility Model Content

[0005] To solve the above-mentioned technical problems, this utility model provides a sepsis laboratory instrument.

[0006] This utility model is achieved through the following technical solution.

[0007] This utility model provides a sepsis laboratory instrument, comprising a first turntable, a connecting rod, a second turntable, a support member, a side plate, a housing, a motor, a power supply, and a rotating shaft. The rotating shaft extends from one side of the housing, passes through the first turntable, and is fixedly connected to the first turntable. One end of the connecting rod is threadedly connected to the rotating shaft, and the other end of the connecting rod is rotatably connected to the support member. The connecting rod passes through the second turntable and is fixedly connected to the second turntable. The support member is connected to the side plate, and one side of the housing is fixedly connected to the side plate. The motor and power supply are disposed inside the housing, the motor output shaft is connected to the rotating shaft, and the power supply is electrically connected to the motor.

[0008] Preferably, the motor output shaft is connected to the rotating shaft via a coupling, the motor and coupling are connected to the housing via a fixing frame, and a regulator is provided on the power supply, with the power supply and regulator being electrically connected.

[0009] Preferably, a support plate is hinged to one side of the outer shell, a storage groove is provided on one side of the outer shell, and a buffer layer is provided on the outer wall of the outer shell.

[0010] Preferably, the support member includes a support rod and a bearing. One end of the support rod is connected to the side plate, and the other end of the support rod is connected to the bearing. The support member is rotatably connected to the connecting rod through the bearing.

[0011] Preferably, the rotating shaft is provided with a first screw hole, and the rotating shaft is threadedly connected to the connecting rod through the first screw hole.

[0012] Preferably, the first turntable is provided with an insertion hole, and the inner wall of the insertion hole is provided with a padding layer.

[0013] Preferably, the side plate is provided with a second screw hole and a mounting hole, and the support member is connected to the side plate through the mounting hole.

[0014] The beneficial effects of this utility model are as follows:

[0015] This invention allows for test tube rotation in either a horizontal or vertical position, depending on the desired rotation direction. The connecting rod, second turntable, and support structure increase the number of test tubes that can be placed, thereby increasing the number of cell cultures and mixed cell-pathogen samples per unit time. Furthermore, the second turntable can be detached when the number of test tubes is small, facilitating the installation of test tubes on the first turntable. The side plates enhance the stability of the device when it is horizontal. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of this utility model when it is laid flat.

[0017] Figure 2 This is a schematic diagram of the structure of this utility model without a second turntable;

[0018] Figure 3 This is a cross-sectional view of the present invention;

[0019] Figure 4 This is a schematic diagram of the structure of this utility model when it is erected;

[0020] In the diagram: 1-First turntable, 11-Shaft, 12-First screw hole, 13-Insertion hole, 14-Padded layer, 2-Connecting rod, 3-Second turntable, 4-Support component, 41-Support rod, 42-Bearing, 5-Side plate, 51-Second screw hole, 52-Mounting hole, 6-Outer shell, 61-Support plate, 62-Storage slot, 63-Buffer layer, 7-Motor, 71-Coupling, 72-Fixed bracket, 8-Power supply, 9-Regulator. Detailed Implementation

[0021] The technical solution of this utility model is further described below, but the scope of protection is not limited to what is described.

[0022] Example 1:

[0023] like Figures 1 to 4 As shown, a sepsis laboratory instrument includes a first turntable 1, a connecting rod 2, a second turntable 3, a support member 4, a side plate 5, a housing 6, a motor 7, a power supply 8, and a rotating shaft 11. The rotating shaft 11 extends from one side of the housing 6, passes through the first turntable 1, and is fixedly connected to the first turntable 1. One end of the connecting rod 2 is threadedly connected to the rotating shaft 11, and the other end of the connecting rod 2 is rotatably connected to the support member 4. The connecting rod 2 passes through the second turntable 3 and is fixedly connected to the second turntable 3. The support member 4 is connected to the side plate 5, and the lower part of one side of the housing 6 is fixedly connected to the side plate 5. The motor 7 and the power supply 8 are disposed inside the housing 6. The output shaft of the motor 7 is connected to the rotating shaft 11, and the power supply 8 is electrically connected to the motor 7.

[0024] When the number of test tubes is small, the connecting rod 2, the second turntable 3 and the support 4 can be disassembled.

[0025] The motor 7 is located in the middle of the housing 6, and the power supply 8 is located inside the housing 6 near the side plate 5. When the side plate 5 is horizontal, it lowers the center of gravity of the instrument and maintains stability. A regulator 9 is installed on the power supply 8, and the power supply 8 and the regulator 9 are electrically connected. It uses a DC power supply with an output voltage of 220V, a US-52 regulator manufactured by Shanghai Xukai Electrical Equipment Co., Ltd., and an M5 motor. The power supply 8 can be connected to an external power socket, and the start / stop and speed of the motor 7 are controlled by the regulator 9. The switch and speed adjustment knob of the regulator 9 are located outside the housing 6.

[0026] The output shaft of the motor 7 is connected to the rotating shaft 11 via a coupling 71, and the motor 7 and the coupling 71 are connected to the inner wall of the outer casing 6 via a fixing frame 72.

[0027] A support plate 61 is hinged to one side of the outer shell 6, and a storage groove 62 is provided on one side of the outer shell 6. A buffer layer 63 is provided on the outer wall of the outer shell 6 to prevent the test tube from touching the outer shell 6 and causing damage. The support plate 61 can be opened when the device is laid flat, providing support and stability. When the device is upright, the support plate 61 can be placed into the storage groove 62, so that the support plate 61 is flush with the outer wall of the outer shell 6.

[0028] The support member 4 includes a support rod 41 and a bearing 42. One end of the support rod 41 is connected to the side plate 5, and the other end of the support rod 41 is connected to the bearing 42. The support member 4 is rotatably connected to the connecting rod 2 through the bearing 42.

[0029] The rotating shaft 11 is provided with a first screw hole 12, and the rotating shaft 11 is threadedly connected to the connecting rod 2 through the first screw hole 12 via an M6 standard thread.

[0030] The first turntable 1 is provided with an insertion hole 13, and a padding layer 14 is provided on the inner wall of the insertion hole 13. The second turntable 3 is the same as the first turntable 1, and is provided with an insertion hole 13 and a padding layer 14. Test tubes can be connected through the insertion hole 13. The padding layer 14 can be made of soft materials such as rubber or plastic, with a thickness of 5mm. The padding layer 14 can maintain the stability of the interference fit between the insertion hole 13 and the test tube, and at the same time play a cushioning role.

[0031] The side plate 5 is provided with a second screw hole 51 and a mounting hole 52. The support rod 41 of the support member 4 is connected to the side plate 5 through the mounting hole 52. The support rod 41 and the mounting hole 52 can be interference-fitted by using soft materials such as rubber or plastic, or an M6 standard thread can be provided in the mounting hole 52 to achieve a threaded connection with the support rod 41.

[0032] When cell culture is required, the device can be placed horizontally and rotated over the culture tubes. By using bolts passing through the second screw hole 51 and connecting to the corresponding screw hole on the work platform, the device can be fixed to the work platform, increasing stability. When mixing cells with pathogens is required, the device can be placed vertically and rotated over the mixing tubes. Therefore, even when the laboratory has only one device, it can meet the above experimental scenarios, improving the efficiency of acquiring effective information during sepsis experiments.

Claims

1. A sepsis experimental apparatus, characterized by: The device includes a first turntable (1), a connecting rod (2), a second turntable (3), a support member (4), a side plate (5), a housing (6), a motor (7), a power supply (8), and a rotating shaft (11). The rotating shaft (11) extends from one side of the housing (6) and passes through the first turntable (1). The rotating shaft (11) is fixedly connected to the first turntable (1). One end of the connecting rod (2) is threadedly connected to the rotating shaft (11), and the other end of the connecting rod (2) is rotatably connected to the support member (4). The connecting rod (2) passes through the second turntable (3) and is fixedly connected to the second turntable (3). The support member (4) is connected to the side plate (5). One side of the housing (6) is fixedly connected to the side plate (5). The motor (7) and the power supply (8) are located inside the housing (6). The output shaft of the motor (7) is connected to the rotating shaft (11), and the power supply (8) is electrically connected to the motor (7).

2. A sepsis testing apparatus as claimed in claim 1, characterized in that: The output shaft of the motor (7) is connected to the rotating shaft (11) through a coupling (71). The motor (7) and the coupling (71) are connected to the outer casing (6) through a fixing frame (72). A regulator (9) is installed on the power supply (8), and the power supply (8) is electrically connected to the regulator (9).

3. A sepsis testing apparatus as claimed in claim 1, characterized in that: A support plate (61) is hinged to one side of the outer shell (6), a storage groove (62) is provided on one side of the outer shell (6), and a buffer layer (63) is provided on the outer wall of the outer shell (6).

4. The sepsis laboratory instrument of claim 1, wherein: The support member (4) includes a support rod (41) and a bearing (42). One end of the support rod (41) is connected to the side plate (5), and the other end of the support rod (41) is connected to the bearing (42). The support member (4) is rotatably connected to the connecting rod (2) through the bearing (42).

5. A sepsis testing apparatus as claimed in claim 1, characterized in that: The rotating shaft (11) is provided with a first screw hole (12), and the rotating shaft (11) is threadedly connected to the connecting rod (2) through the first screw hole (12).

6. A sepsis testing apparatus as claimed in claim 1, characterized in that: The first turntable (1) is provided with a socket (13), and the inner wall of the socket (13) is provided with a pad (14).

7. A sepsis testing apparatus as claimed in claim 1, characterized in that: The side plate (5) is provided with a second screw hole (51) and a mounting hole (52), and the support member (4) is connected to the side plate (5) through the mounting hole (52).

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