Constant temperature device for observing states of adherent cells under microscope

Abstract

The utility model provides a constant temperature device for observing the state of adherent cells under a microscope, which comprises a transparent box body, the transparent box body comprises an upper transparent plate and a lower transparent plate, a temperature sensor is arranged between the two transparent plates, and the front side and the rear side of the two transparent plates are connected with a constant temperature heater in a sealing mode. A left transparent side plate is hermetically connected to the left sides of the two transparent plates; a transparent sealing flip cover is arranged on the right side of the transparent box body; the constant-temperature heater on the front side is provided with a control panel, a control circuit board is installed in the control panel, the temperature sensor and the constant-temperature heater are both connected with the control circuit board, and a power switch, a warning lamp, a buzzer, control keys and a digital display screen are arranged on the front face of the control panel. By means of the constant temperature device, the state of the adherent cells can be observed in real time under a microscope, the adherent cells do not need to be separated from a culture environment, and inaccuracy of results caused by different observation time is avoided.

Description

Technical Field

[0001] This utility model relates to biochemical detection, specifically to a constant temperature device for observing the state of adherent cells under a microscope. Background Technology

[0002] Cell adhesion refers to the process by which adherent cells attach to and spread on a culture surface. The tightness of cell adhesion depends on the characteristics of the cells themselves, the probability of cell-culture surface contact, and the compatibility between the cells and the culture surface.

[0003] In traditional adherent cell culture, once cells reach a suitable degree of confluence, a digestion solution is added to detach them from the culture vessel. The termination of digestion is primarily assessed through manual observation using an inverted microscope. If digestion is incomplete, cells must be transferred to an incubator for further digestion. This process is cumbersome, hinders the accurate determination of digestion time, and easily leads to over-digestion. The success of the adherent cell digestion process directly impacts the viability of the cultured cells.

[0004] In practical use, the main technical problem is that many adherent cells grow in cell culture plates. The adhesion strength of adherent cells is affected by the cell's own characteristics, the probability of cell-culture surface contact, and the compatibility between the cell and the culture surface. The digestion time varies depending on the cell's growth stage. The traditional digestion method involves adding cell digestion solution to the wells, placing them in a constant temperature incubator for a certain time, and then observing them under a microscope. However, the digestion status cannot be observed during the constant temperature digestion period. If digestion is incomplete, the cells must be placed back in the incubator, posing a risk of over-digestion. Temperature has a significant impact on cell growth, and different observation times when adhering cells are removed from the culture environment can lead to inaccurate results. Utility Model Content

[0005] This invention provides a thermostat for observing adherent cells under a microscope. The thermostat is detachably placed on the microscope stage. The thermostat comprises a transparent housing containing two parallel, spaced-apart transparent plates. A temperature sensor is installed between the two transparent plates.

[0006] The transparent box is equipped with constant temperature heaters that are sealed and connected to the two transparent panels on both the front and rear sides. A left transparent side panel is provided on the left side of the transparent box and sealed and connected to the constant temperature heaters and the two transparent panels. A transparent sealed flip cover is provided on the right side of the transparent box. The upper edge of the transparent sealed flip cover is rotatably connected to the upper transparent panel, and the lower edge of the transparent sealed flip cover is magnetically connected to the lower transparent panel.

[0007] The front of the constant temperature heater is equipped with a control panel, and a control circuit board is installed in the control panel. The temperature sensor and the constant temperature heater are both connected to the control circuit board. The front of the control panel is equipped with a power switch, warning light, buzzer, control buttons, and digital display screen connected to the control circuit board. The control buttons include a timing button and a heating button for adjusting the power of the constant temperature heater.

[0008] Furthermore, the two transparent plates and the transparent sealing flip cover are optically high-transmittance glass with a light transmittance greater than 95%.

[0009] Furthermore, the upper surface of the microscope stage is provided with a positioning groove, and the temperature control device is placed in the positioning groove.

[0010] Furthermore, the thermostatic heaters on the front and rear sides are connected in series.

[0011] Furthermore, the upper edge of the transparent sealing flip cover is rotatably connected to the upper transparent plate via a hinge.

[0012] Furthermore, the transparent sealing flip cover is provided with felt sealing strips on the sides of the two transparent plates and the contact end face of the constant temperature heater.

[0013] This invention provides a thermostat for observing adherent cells under a microscope, allowing for real-time observation without removing the cells from the culture environment, thus avoiding inaccuracies caused by varying observation times. The digital display on the control panel shows the temperature inside the transparent chamber in real time, and the power of the thermostat heater can be adjusted via control buttons, precisely controlling the temperature and preventing temperature-related effects on cell growth. Furthermore, the transparent, sealed flip-top design facilitates opening and closing, allowing for easy manipulation of the adherent cells within the chamber, and provides excellent sealing to maintain a constant temperature environment. The transparent chamber, left transparent side panel, and transparent sealing flip-top are all made of high-transmittance optical glass with a light transmittance greater than 95%, ensuring clear observation under the microscope. The thermostat is detachably placed in a positioning groove on the microscope stage, facilitating installation and removal and improving ease of use. Attached Figure Description

[0014] To more clearly illustrate the technical solutions in the embodiments of this utility model, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0015] Figure 1A simplified structural diagram of a thermostat for observing adherent cells under a microscope, provided by this utility model. Detailed Implementation

[0016] In the following description, numerous specific details are set forth in order to provide a more thorough understanding of the present invention. However, it will be apparent to those skilled in the art that the present invention can be practiced without one or more of these details. In other instances, certain technical features well-known in the art have not been described in order to avoid confusion with the present invention.

[0017] To fully understand this utility model, detailed steps and structures will be presented in the following description to illustrate the technical solution of this utility model. Preferred embodiments of this utility model are described in detail below; however, in addition to these detailed descriptions, this utility model may have other embodiments.

[0018] Reference Figure 1 As shown, this utility model provides a thermostat for observing adherent cells under a microscope. The thermostat can be detachably placed on the microscope stage. The thermostat includes a transparent box, which contains two transparent plates 6 that are arranged parallel to each other and spaced apart. A temperature sensor is installed between the two transparent plates 6.

[0019] The transparent box is equipped with constant temperature heaters 7 on both the front and rear sides, which are sealed and connected to the two transparent plates 6. On the left side of the transparent box, there is a left transparent side plate that is sealed and connected to the constant temperature heaters 7 and the two transparent plates 6. On the right side of the transparent box, there is a transparent sealing flip cover 8. The upper edge of the transparent sealing flip cover 8 is rotatably connected to the upper transparent plate, and the lower edge of the transparent sealing flip cover 8 is magnetically connected to the lower transparent plate.

[0020] The front of the thermostatic heater 7 is equipped with a control panel, which contains a control circuit board. The temperature sensor and the thermostatic heater 7 are both connected to the control circuit board. The front of the control panel is equipped with a power switch 1, a warning light 2, a buzzer 3, control buttons 4, and a digital display screen 5, all connected to the control circuit board. The control buttons 4 include a timing button and a heating button for adjusting the power of the thermostatic heater 7.

[0021] The time and overall device temperature are set via control button 4, while the digital display screen 5 shows the time and current device temperature in real time. A red warning light 2 and a buzzer 3 alert the operator to stop operation promptly. This device eliminates the need for multiple transfers of cell culture plates between the incubator and microscope, reducing the risk of contamination. The constant-temperature, sealed environment fully utilizes the digestion solution's properties. The alarm system alerts the operator, and continuous microscopic observation allows for convenient control of cell digestion, preventing incomplete or excessive digestion. It can also maintain the culture temperature during microscopic observation of cells.

[0022] In an optional embodiment, the two transparent plates 6 and the transparent sealing flip cover 8 are made of optically high-transmittance glass with a light transmittance greater than 95% to ensure clarity of observation under the microscope. The internal space of the transparent box can be customized according to the size of the microscope stage and the number of cell culture wells to be observed to meet different experimental needs.

[0023] In an optional embodiment, the upper surface of the microscope stage is provided with a positioning groove, the thermostat is placed in the positioning groove, and the bottom of the positioning groove is provided with an anti-slip pad made of rubber to increase the friction between the thermostat and the microscope stage, prevent the thermostat from sliding or shifting during observation, and ensure the accuracy and stability of observation.

[0024] In an optional embodiment, the thermostatic heaters 7 on the front and rear sides are connected in series to ensure that the two thermostatic heaters 7 have the same power, achieve uniform heating, and avoid excessive temperature difference between the front and rear sides of the box.

[0025] In an optional embodiment, the upper edge of the transparent sealing flip cover 8 is rotatably connected to the upper transparent plate via a hinge 9. Felt sealing strips are provided on the contact surfaces of the transparent sealing flip cover 8, the sides of the two transparent plates 6, and the contact surfaces of the constant temperature heater 7 to improve sealing performance. A handle 10 is provided on the right side of the transparent sealing flip cover 8 for easy opening.

[0026] The method of using this utility model is as follows:

[0027] When using this device, first sterilize it and place it on the microscope stage in the cell culture workshop to put the microscope into working condition. Then turn on the power switch 1 and adjust the device temperature and time through the control panel 4. Preheat for more than 15 minutes.

[0028] Then open the sealing flap 8, push the cell culture container into the device, and close the sealing flap 8. The operator can observe the cell status in real time through the transparent plate 6 under a microscope. If the observation is in the digestion stage, the red warning light 2 will flash and the buzzer 3 will sound an alarm after the digestion timer is completed to avoid incomplete or excessive digestion. After digestion is complete, subsequent operations can be performed.

[0029] The entire process is simple and convenient, with digestion under constant temperature conditions, allowing for continuous microscopic observation, reducing the risk of sample contamination and avoiding incomplete or excessive cell digestion. For observation of cells in culture, the temperature can be kept constant during the removal from the culture environment, and the removal time can be standardized to improve the consistency of results.

[0030] In summary, the constant temperature device provided by this invention not only solves the problem of not being able to observe cell state in real time during traditional cell digestion, but also improves the accuracy and efficiency of cell digestion. Through the cooperation of the constant temperature heater and the control circuit board, the temperature inside the device can be precisely controlled, ensuring that cells are digested and observed under optimal temperature conditions. At the same time, the transparent chamber design allows operators to directly observe the cell state under a microscope without removing the cells from the culture environment, thus avoiding the impact of temperature changes on cell growth.

[0031] The preferred embodiments of this utility model have been described above. It should be understood that this utility model is not limited to the specific embodiments described above. Devices and structures not described in detail herein should be understood as being implemented in a conventional manner within the art. Any person skilled in the art can make many possible variations and modifications to the technical solutions of this utility model using the disclosed methods and techniques, or modify them into equivalent embodiments with equivalent changes, without departing from the scope of the technical solution of this utility model. This does not affect the essential content of this utility model. Therefore, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of this utility model, without departing from the content of the technical solution of this utility model, still fall within the protection scope of the technical solution of this utility model.

Claims

1. A thermostat device for observing the state of adherent cells under a microscope, the thermostat device being detachably placed on a microscope stage, characterized in that, The temperature control device includes a transparent housing, which contains two transparent plates (6) arranged parallel to each other vertically and spaced apart. A temperature sensor is installed between the two transparent plates (6). The front and rear sides of the transparent box are equipped with constant temperature heaters (7) that are sealed and connected to the two transparent plates (6). On the left side of the transparent box, there is a left transparent side plate that is sealed and connected to the constant temperature heater (7) and the two transparent plates (6). On the right side of the transparent box, there is a transparent sealing flip cover (8). The upper edge of the transparent sealing flip cover (8) is rotatably connected to the upper transparent plate, and the lower edge of the transparent sealing flip cover (8) is magnetically connected to the lower transparent plate. The front of the constant temperature heater (7) is equipped with a control panel. The control panel contains a control circuit board. The temperature sensor and the constant temperature heater (7) are both connected to the control circuit board. The front of the control panel is equipped with a power switch (1), a warning light (2), a buzzer (3), control buttons (4), and a digital display screen (5) connected to the control circuit board. The control buttons (4) include a timing button and a heating button for adjusting the power of the constant temperature heater (7).

2. A thermostat device for observing the state of adherent cells under a microscope according to claim 1, wherein The two transparent plates (6) and the transparent sealing flip cover (8) are optically high-transmittance glass with a light transmittance of more than 95%.

3. A thermostat device for observing the state of adherent cells under a microscope according to claim 1, wherein The upper surface of the microscope stage is provided with a positioning groove, and the temperature control device is placed in the positioning groove.

4. A thermostat device for observing the state of adherent cells under a microscope according to claim 1, wherein The constant temperature heaters (7) on the front and rear sides are connected in series.

5. A thermostat device for observing the state of adherent cells under a microscope according to claim 1, wherein The upper edge of the transparent sealing flip cover (8) is rotatably connected to the upper transparent plate via a hinge (9).

6. A thermostat device for observing the state of adherent cells under a microscope according to claim 5, wherein The transparent sealing flip cover (8) is provided with felt sealing strips on the side surfaces of the two transparent plates (6) and the contact surfaces of the constant temperature heater (7).

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