NK cell cryopreservation equipment

Abstract

The utility model discloses NK (Natural Killer) cell cryopreservation equipment, which relates to the technical field of biomedicine and comprises a cryopreservation box, a first sliding seat, a second sliding seat, a bearing cylinder, a guide rod, a limiting clamping ring, a reset spring, a bearing back cushion and an end cover. The cryopreservation box is a main body shell of the equipment, and a plurality of sliding grooves are formed in the inner wall of the cryopreservation box and used for guiding the first sliding base and the second sliding base to move along a preset track and achieving accurate positioning. Furthermore, the first sliding seat and the second sliding seat slide up and down along the inner wall of the cryopreservation box through sliding grooves so as to adapt to test tubes with different lengths and fix the test tubes, and the first sliding seat comprises a first fixing block, a first threaded cylinder, a first screw rod, a bearing cylinder and a guide rod. According to the NK cell cryopreservation equipment provided by the utility model, the test tube is stably fixed through the matching of the sliding seat and the bearing cylinder, so that the test tube is prevented from being broken or NK cells are prevented from leaking in the cryopreservation process, and meanwhile, the operation convenience and the equipment universality are improved.

Description

Technical Field

[0001] This utility model relates to the field of biomedical technology, specifically to an NK cell cryopreservation device. Background Technology

[0002] In the fields of biomedicine and cell storage, NK cell cryopreservation technology has significant research value and application prospects. As an important component of the immune system, NK cells play a crucial role in tumor treatment, immune regulation, and clinical research on various diseases. To ensure the activity and integrity of NK cells during cryopreservation, they typically need to be placed in test tubes and stored in specialized cryopreservation equipment. However, current cryopreservation equipment has many shortcomings in practical use, making it difficult to meet the needs for efficient and safe cryopreservation.

[0003] Existing cryopreservation equipment performs poorly in securing test tubes, often failing to hold them firmly in the cryovial. Due to the lack of effective securing mechanisms, test tubes are easily broken during cryopreservation due to shaking or impact, leading to the loss of NK cell samples. Furthermore, insufficient sealing or insecure securing of the test tubes can cause cell leakage, wasting valuable biological samples and potentially contaminating the cryopreservation environment. Simultaneously, existing equipment also suffers from significant operational shortcomings; for example, placing test tubes one by one into the cryovial is a cumbersome and inefficient process, increasing the workload of laboratory personnel.

[0004] The aforementioned problems severely impact the reliability and efficiency of NK cell cryopreservation, hindering the development and application of related technologies. Therefore, there is an urgent need for a novel NK cell cryopreservation device that can effectively solve these problems, achieving stable fixation of test tubes, preventing cell spillage, and improving operational convenience, thereby providing technical support for the long-term preservation and efficient utilization of NK cells. Utility Model Content

[0005] This invention addresses the technical problems of unstable test tube fixation, easy breakage, NK cell leakage, and inconvenient test tube placement in existing technologies. Therefore, this invention adopts the following technical solution:

[0006] This invention provides an NK cell cryopreservation device, including a cryopreservation box, a first sliding seat, a second sliding seat, a support cylinder, a guide rod, a limiting ring, a return spring, a support pad, and an end cap. The cryopreservation box is the main outer shell of the device, and its inner wall is provided with multiple sliding grooves for guiding the first and second sliding seats to move along a predetermined trajectory and achieve precise positioning. Furthermore, the first and second sliding seats slide up and down along the inner wall of the cryopreservation box via the sliding grooves to accommodate and fix test tubes of different lengths.

[0007] The first sliding seat includes a first fixed block, a first threaded cylinder, a first screw, a support cylinder, and a guide rod; the second sliding seat includes a second fixed block, a second threaded cylinder, a second screw, and a limiting retaining ring. The first threaded cylinder and the first screw constitute a threaded transmission mechanism, allowing the height of the first sliding seat to be adjusted by rotating the first screw. Similarly, the second threaded cylinder and the second screw constitute a threaded transmission mechanism for adjusting the height of the second sliding seat. Specifically, the height adjustment range of the first and second sliding seats is limited by the length of the slide groove, thereby ensuring stable movement within the cryogenic storage box.

[0008] Furthermore, the support cylinder directly supports the bottom of the test tube. An internal guide rod is installed inside the cylinder, penetrating it and connecting to the first sliding seat, ensuring the support cylinder remains vertical. The linear movement of the guide rod limits the range of motion of the support cylinder, preventing the test tube from tilting or shifting during cryopreservation. Specifically, the inner wall of the support cylinder is provided with a support pad made of elastic material with an anti-slip texture, increasing friction to prevent the test tube from sliding or shifting during cryopreservation.

[0009] The limiting ring is mounted on the second sliding seat to restrict the lifting range of the support cylinder, preventing damage to the test tubes due to excessive movement. The relative position between the limiting ring and the support cylinder is adjusted by the second screw to accommodate test tubes of different lengths. Furthermore, a return spring is positioned between the first sliding seat and the support cylinder, with one end fixed to the first fixing block of the first sliding seat and the other end contacting the bottom of the support cylinder. When no external force is applied, the return spring pushes the support cylinder back to its initial position, thus simplifying the removal of the test tubes.

[0010] Specifically, the end cap covers the top of the test tube, and a sealing ring made of flexible material is provided on the inner side of the end cap to form a tight fit with the mouth of the test tube, preventing NK cells from leaking during cryopreservation. The end cap is connected to the test tube by a threaded or snap-fit ​​structure to ensure its stability during cryopreservation.

[0011] This utility model solves the technical problems existing in the prior art through the above-described specific structural design, specifically in the following aspects:

[0012] S1. Through the cooperation of the first and second sliding seats, combined with the design of the support cylinder and guide rod, the test tube is firmly fixed in the cryopreservation box. The linear movement of the guide rod limits the sway range of the support cylinder, preventing the test tube from tilting or shifting during cryopreservation.

[0013] S2. The anti-slip function of the support pad, combined with the sealing design of the end cap, effectively prevents leakage of NK cells during cryopreservation. The elastic material of the support pad absorbs external impacts, further protecting the test tubes from damage.

[0014] S3. The design of the chute and sliding seat allows test tubes to be placed and removed one by one, simplifying operation and improving work efficiency. The inner wall of the chute is coated with a low-friction coating to reduce resistance when the sliding seat moves, ensuring smooth operation.

[0015] S4. The height adjustment function of the first and second screws allows the equipment to adapt to test tubes of different lengths, enhancing its versatility. The accuracy of the threaded transmission mechanism is determined by the thread pitch, ensuring accurate height adjustment.

[0016] S5. The design of the limit ring and return spring enhances the safety of the equipment. The limit ring restricts the lifting range of the support cylinder, preventing damage to the test tubes due to misoperation; the return spring simplifies the removal of the test tubes, ensuring ease of operation.

[0017] The beneficial effects of this invention are as follows: By optimizing the structural design of the cryopreservation box, the test tubes are securely fixed, preventing breakage or leakage of NK cells during cryopreservation. Specifically, the cooperation between the support cylinder and the guide rod ensures the vertical position of the test tubes during cryopreservation, avoiding damage caused by vibration or external impact. Simultaneously, the anti-slip function of the support pad combined with the sealing design of the end cap further enhances the reliability of the equipment. Furthermore, the design of the sliding groove and sliding seat allows for the individual placement and removal of test tubes, significantly improving operational efficiency. The height adjustment function of the first and second screws allows the equipment to accommodate test tubes of different lengths, enhancing its flexibility in practical applications. The design of the limiting retaining ring and the return spring further improves the safety of the equipment, preventing test tube damage due to misoperation.

[0018] In summary, the NK cell cryopreservation device provided by this utility model solves the technical problems existing in the prior art through specific structural design and technical implementation. It has the characteristics of reasonable structure, convenient operation, safety and reliability, and is suitable for a wide range of applications in the biomedical field. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this utility model. For those skilled in the art, other drawings can be obtained based on these drawings.

[0020] Figure 1 A schematic diagram of the overall structure provided for an embodiment of this utility model;

[0021] Figure 2 Provided for the embodiments of this utility model Figure 1 Partial disassembly diagram;

[0022] Figure 3 Provided for the embodiments of this utility model Figure 2 A partial structural diagram;

[0023] Figure 4 Provided for the embodiments of this utility model Figure 3 Schematic diagram of the structure at point A;

[0024] Figure 5 Provided for the embodiments of this utility model Figure 2 Partial disassembly diagram;

[0025] Figure 6 Provided for the embodiments of this utility model Figure 5 A schematic diagram of the structure at point B.

[0026] Explanation of reference numerals in the attached figures:

[0027] 1. Cryogenic box; 2. Slide groove; 3. First sliding seat; 31. First fixing block; 32. First threaded cylinder; 33. First screw; 34. Support cylinder; 35. Guide rod; 4. Second sliding seat; 41. Second fixing block; 42. Second threaded cylinder; 43. Second screw; 44. Limiting ring; 5. Return spring; 6. Supporting pad; 71. Test tube; 72. End cap. Detailed Implementation

[0028] To enable those skilled in the art to better understand the technical solution of this utility model, the present utility model will be further described in detail below with reference to the accompanying drawings.

[0029] This utility model provides an NK cell cryopreservation device, the specific implementation of which is described in conjunction with the appendix. Figure 1 To be continued Figure 6 A detailed description is provided. The device includes a cryopreservation box 1, a sliding groove 2, a first sliding seat 3, a second sliding seat 4, a support cylinder 34, a guide rod 35, a limiting retaining ring 44, a return spring 5, a supporting pad 6, a test tube 71, and an end cap 72. Through the synergistic effect of the above structures, the test tube 71 is stably fixed and protected during NK cell cryopreservation, solving the problems of test tube breakage, NK cell leakage, and inconvenient test tube placement in existing technologies.

[0030] The cryopreservation chamber 1 is the main outer shell of the device, used to house all internal components and provide a low-temperature environment for the cryopreservation of NK cells. The inner wall of the cryopreservation chamber 1 is provided with multiple sliding grooves 2, which extend vertically and penetrate the internal space of the cryopreservation chamber 1. The inner wall of the sliding grooves 2 is treated with a low-friction coating to ensure low resistance during movement of the sliding components, while preventing wear and tear due to long-term use. The design of the sliding grooves 2 not only provides a stable movement trajectory for the first sliding seat 3 and the second sliding seat 4, but also enhances the safety of the device by limiting the sliding range.

[0031] The first sliding seat 3 and the second sliding seat 4 are respectively installed in the slide groove 2, and both can slide up and down along the slide groove 2. The first sliding seat 3 includes a first fixing block 31, a first threaded cylinder 32, a first screw 33, a support cylinder 34, and a guide rod 35. The first fixing block 31 is fixed to the slide groove 2, and the first threaded cylinder 32 is embedded in the first fixing block 31, forming a threaded transmission mechanism with the first screw 33. By rotating the first screw 33, the first sliding seat 3 can move up and down along the slide groove 2, thereby adjusting the height of the support cylinder 34. The pitch of the first screw 33 is precisely designed, ensuring high precision and stability in the height adjustment process. The guide rod 35 passes through the support cylinder 34 and connects to the first sliding seat 3, ensuring that the support cylinder 34 always remains vertical. The linear movement of the guide rod 35 limits the range of motion of the support cylinder 34, preventing the test tube 71 from tilting or shifting during cryopreservation.

[0032] The support cylinder 34 directly supports the bottom of the test tube 71, and its inner wall is provided with a support pad 6. The support pad 6 is made of elastic material with a non-slip texture on the surface, and fits tightly against the outer wall of the test tube 71. The elastic properties of the support pad 6 can absorb external impacts and prevent the test tube 71 from being damaged by vibration or collision. In addition, the non-slip texture of the support pad 6 increases friction, further improving the stability of the test tube 71 during cryopreservation.

[0033] The second sliding seat 4 includes a second fixed block 41, a second threaded cylinder 42, a second screw 43, and a limiting ring 44. The second fixed block 41 is fixed to the slide groove 2, and the second threaded cylinder 42 is embedded in the second fixed block 41, forming a threaded transmission mechanism with the second screw 43. By rotating the second screw 43, the second sliding seat 4 can move up and down along the slide groove 2, thereby adjusting the height of the limiting ring 44. The limiting ring 44 is provided on the second sliding seat 4 to limit the lifting range of the support cylinder 34, preventing damage to the test tube 71 due to excessive movement. The relative position between the limiting ring 44 and the support cylinder 34 is adjusted by the second screw 43 to accommodate test tubes 71 of different lengths. This adjustment method allows the equipment to flexibly handle test tubes 71 of various specifications, enhancing the equipment's versatility.

[0034] A return spring 5 is positioned between the first sliding seat 3 and the support cylinder 34. One end of the return spring 5 is fixed to the first fixing block 31 of the first sliding seat 3, and the other end contacts the bottom of the support cylinder 34. When no external force is applied, the return spring 5 pushes the support cylinder 34 back to its initial position, thereby simplifying the removal of the test tube 71. The design of the return spring 5 not only improves the convenience of operation but also reduces the need for manual intervention to a certain extent, thus improving work efficiency.

[0035] End cap 72 covers the top of test tube 71, and a sealing ring is provided on its inner side. The sealing ring is made of flexible material and forms a tight fit with the mouth of test tube 71 to prevent NK cells from leaking during cryopreservation. End cap 72 is connected to test tube 71 by a threaded or snap-fit ​​structure to ensure its stability during cryopreservation. The sealing design of end cap 72, combined with the anti-slip function of support pad 6, effectively solves the technical problem of NK cell leakage.

[0036] In practical applications, the operator first places the test tube 71 inside the support cylinder 34. The elastic material of the support pad 6 automatically conforms to the outer wall of the test tube 71, forming a stable support. Then, the operator adjusts the height of the first sliding seat 3 and the second sliding seat 4 by rotating the first screw 33 and the second screw 43, respectively, so that the distance between the limiting ring 44 and the support cylinder 34 matches the length of the test tube 71. After adjustment, the limiting ring 44 limits the upper part of the test tube 71, preventing it from shifting due to vibration or external impact. At this point, the end cap 72 is tightened or fastened to the top of the test tube 71, completing the sealing operation.

[0037] The low-temperature environment inside the cryopreservation box 1 is maintained by the refrigeration system, keeping the test tube 71 stable during cryopreservation. To remove the test tube 71, the operator simply loosens the end cap 72 and rotates the first screw 33 and the second screw 43 in the opposite direction, returning the first sliding seat 3 and the second sliding seat 4 to their initial positions. During this process, the return spring 5 automatically pushes the support cylinder 34 upwards, facilitating quick removal of the test tube 71 by the operator.

[0038] This utility model solves the technical problems existing in the prior art through the above-described specific structural design and technical implementation. First, the cooperation of the first sliding seat 3 and the second sliding seat 4, combined with the design of the support cylinder 34 and the guide rod 35, firmly fixes the test tube 71 in the cryopreservation box 1, avoiding damage to the test tube 71 due to vibration or external impact. Second, the anti-slip function of the support pad 6 combined with the sealing design of the end cap 72 effectively prevents leakage of NK cells during cryopreservation. Third, the design of the slide groove 2 and the sliding seat allows the test tube 71 to be placed and removed one by one, significantly improving operational efficiency. Finally, the height adjustment function of the first screw 33 and the second screw 43 allows the equipment to adapt to test tubes 71 of different lengths, enhancing the versatility of the equipment. The design of the limiting ring 44 and the return spring 5 further enhances the safety of the equipment, preventing damage to the test tube 71 due to misoperation.

[0039] In summary, the NK cell cryopreservation device provided by this utility model solves the technical problems existing in the prior art through specific structural design and technical implementation. It has the characteristics of reasonable structure, convenient operation, safety and reliability, and is suitable for a wide range of applications in the biomedical field.

[0040] The foregoing description only illustrates certain exemplary embodiments of the present invention. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. An NK cell cryopreservation apparatus, characterized by, The system includes a cryopreservation box (1), a first sliding seat (3), a second sliding seat (4), a support cylinder (34), a guide rod (35), a limiting ring (44), a return spring (5), a support cushion (6), and an end cap (72). The inner wall of the cryopreservation box (1) is provided with multiple sliding grooves (2). The first sliding seat (3) and the second sliding seat (4) slide up and down along the inner wall of the cryopreservation box (1) through the sliding grooves (2). The support cylinder (34) is used to support the bottom of the test tube (71) and is connected to the guide rod (35). The limiting ring (44) is set on the second sliding seat (4). The return spring (5) is set between the first sliding seat (3) and the support cylinder (34). The end cap (72) covers the top of the test tube (71).

2. The NK cell cryopreservation device according to claim 1, characterized in that, The first sliding seat (3) includes a first fixed block (31), a first threaded cylinder (32), and a first screw (33). The first threaded cylinder (32) and the first screw (33) constitute a threaded transmission mechanism to adjust the height of the first sliding seat (3).

3. The NK cell cryo device of claim 2, wherein, The pitch of the first screw (33) is precisely designed to ensure the stability of height adjustment.

4. The NK cell cryo-device of claim 1, wherein, The second sliding seat (4) includes a second fixed block (41), a second threaded cylinder (42), and a second screw (43). The second threaded cylinder (42) and the second screw (43) constitute a threaded transmission mechanism to adjust the height of the second sliding seat (4).

5. The NK cell cryo device of claim 4, wherein, The relative position between the limiting ring (44) and the support cylinder (34) is adjusted by the second screw (43) to accommodate test tubes (71) of different lengths.

6. The NK cell cryo-device of claim 1, wherein, The inner wall of the support cylinder (34) is provided with a support cushion (6), which is made of elastic material and has an anti-slip texture on the surface.

7. The NK cell cryo-device of claim 1, wherein, A sealing ring is provided on the inner side of the end cap (72), the sealing ring is made of flexible material and fits tightly against the mouth of the test tube (71).

8. The NK cell cryo-device of claim 1, wherein, The inner wall of the groove (2) is provided with a low-friction coating to reduce the resistance when the sliding seat moves.

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