Sterile refrigerant preparation device

Abstract

The utility model discloses a sterile refrigerant preparation device, and belongs to the technical field of biological sample preservation, the sterile refrigerant preparation device comprises a bearing part floating on the upper surface of liquid nitrogen, the bearing part comprises an upper plate 2 and a lower plate 3 which are connected through an adjusting part capable of adjusting the connection distance, and a sterile container 1 is loaded between the upper plate 2 and the lower plate 3. The upper plate 2 is made of a material with buoyancy, and the adjusting part is a screw nut and is used for preparing a sterile container 1 for loading a sterile refrigerant and loading the sterile container 1 on the bearing part. The sterile container is used for loading the prepared sterile refrigerant, the technical problem that the preparation dosage is unstable due to the fact that the liquid level of the liquid nitrogen drops when the sterile container is used for preparing the liquid nitrogen is solved, and the sterile container is mainly applied to preparation of the liquid nitrogen with the stable dosage.

Description

Technical Field

[0001] This invention belongs to the field of biological sample preservation technology, specifically relating to a sterile refrigerant preparation device. Background Technology

[0002] In assisted reproductive technology (ART), cryopreservation of cell samples such as embryos and eggs is necessary, with liquid nitrogen being the primary cold source. The potential contamination risks associated with vitrification have long troubled ART practitioners. During vitrification, embryo samples mounted on cryopreservation carriers come into direct contact with cold sources such as liquid nitrogen. However, commercially available liquid nitrogen and other cold sources can be contaminated by pathogenic microorganisms such as bacteria, viruses, and fungi during production, transportation, and long-term storage in liquid nitrogen tanks or other storage containers. Pathogenic microorganisms can tolerate low temperatures and pose a risk of contamination and cross-infection after thawing; therefore, the development of sterile refrigerants is necessary.

[0003] Chinese Patent Publication No. CN110521720A discloses a method for ultra-low temperature freezing and closed-loop preservation, which requires a specific preparation device. In rod-based freezing operations, a certain volume of sterile liquid nitrogen needs to be prepared in accordance with the freezing time to meet the requirements of closed-loop vitrification freezing. However, existing supports or devices are fixed in a certain position, and different sterile containers have different depths. Furthermore, as liquid nitrogen is consumed during freezing, it is impossible to prepare a stable volume of liquid nitrogen within a fixed time. In closed-loop vitrification freezing, if too much liquid nitrogen is sealed, it will rapidly vaporize during rewarming, easily causing the opening to burst and resulting in the loss of biological samples. If the prepared liquid nitrogen volume is too small, the cooling rate is slow, leading to a low thawing survival rate. Utility Model Content

[0004] Purpose of the invention: The purpose of this invention is to provide a sterile refrigerant preparation device to solve the technical problem of unstable preparation dosage caused by the drop in liquid nitrogen level when preparing liquid nitrogen in a sterile container.

[0005] The above-mentioned technical objective of the present invention is achieved through the following technical solution: a sterile refrigerant preparation apparatus, characterized in that it includes a support component floating on the surface of liquid nitrogen, and a sterile container for preparing and loading sterile refrigerant is loaded onto the support component. The sterile container is used to load the prepared sterile refrigerant.

[0006] Furthermore: the supporting component consists of an upper plate and a lower plate connected by an adjustable connection distance, and the sterile container is loaded between the upper plate and the lower plate.

[0007] Furthermore, the upper plate is made of a buoyant material.

[0008] Furthermore, the adjusting component is a screw and nut.

[0009] Furthermore, it also includes a float plate, which is mounted on the screw.

[0010] Compared with the prior art, the present invention has the following advantages: by using a floating plate or a buoyancy material to make the upper plate, the opening of the sterile container is always kept in the vapor layer of liquid nitrogen, thereby preparing a stable volume of liquid nitrogen within a fixed time. Since the structure of the present application is more reasonable, the production cost is lower, making it suitable for widespread promotion and application. Attached Figure Description

[0011] Figure 1 This is a structural diagram of the utility model. Detailed Implementation

[0012] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0013] Example 1:

[0014] In one technical solution of the present invention, such as Figure 1 As shown, a sterile refrigerant preparation apparatus is characterized by comprising a support component floating on the surface of liquid nitrogen. The support component consists of an upper plate 2 and a lower plate 3 connected by an adjustable connection component. A sterile container 1 is loaded between the upper plate 2 and the lower plate 3. The upper plate 2 is made of a buoyant material. The adjustable component is a screw and nut used to prepare the sterile container 1 for loading the sterile refrigerant onto the support component. The sterile container is used to load the prepared sterile refrigerant.

[0015] In the above technical solution: a sterile container 1 is inserted between the upper plate 2 and the lower plate 3, and the entire device is placed in liquid nitrogen. The upper plate 2 provides buoyancy and is always flush with the liquid nitrogen surface. The lower plate 3 is immersed in the liquid nitrogen. Above the liquid nitrogen surface is a vapor layer. The opening of the sterile container 1 is higher than the vapor layer. The part of the sterile container 1 located between the upper plate 2 and the lower plate 3 is immersed in the liquid nitrogen surface along with the entire device, so that the temperature of the inner wall of the sterile container is infinitely close to the temperature of the liquid nitrogen. The air in the sterile container condenses and liquefies at this temperature. As the air liquefies, a negative pressure is formed in the sterile container. The nitrogen from the external air or the vapor layer enters the sterile container and liquefies. As the liquid nitrogen vaporizes and the liquid level drops, the upper plate 2 and the lower plate 3 drop with the liquid level, keeping the height of immersion in the liquid level constant. This achieves stable preparation of liquid nitrogen within a fixed time, unaffected by the vaporization of liquid nitrogen.

[0016] In another technical solution, a float plate 4 is also included. The float plate 4 is mounted on the screw and provides buoyancy so that the upper plate 2 is always above the liquid surface.

[0017] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements can be made without departing from the principle of the present invention, and these improvements should also be considered within the scope of protection of the present invention.

Claims

1. A sterile refrigerant preparation device, characterized by, Includes a support component floating on the surface of liquid nitrogen, and a sterile container (1) for preparing a sterile refrigerant loaded on the support component. The sterile container is used to load the prepared sterile refrigerant. The supporting component consists of an upper plate (2) and a lower plate (3) connected by an adjustable connection distance, and the sterile container (1) is loaded between the upper plate (2) and the lower plate (3).

2. A sterile refrigerant preparation apparatus as claimed in claim 1, characterized in that: The upper plate (2) is made of a buoyant material.

3. A sterile refrigerant preparation apparatus as defined in claim 1, wherein: The adjusting component is a screw and nut.

4. A sterile refrigerant preparation apparatus as claimed in claim 3, wherein: It also includes a float plate (4), which is loaded onto the screw.

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