Device for simulating root system secretion and soil gas collection

Abstract

The utility model provides a device for simulating root system secretion and soil gas collection, which comprises a culture device with soil inside; an opening is formed in the bottom of the gas collecting device; the base is connected with the bottom of the gas collecting device; a root system simulation unit is arranged in the culture device, at least part of the root system simulation unit is buried in soil, and the root system simulation unit discharges secretions into the soil; the gas collection device is buckled on the culture device through the opening, a gas collection channel is arranged at the top of the gas collection device and is used for collecting gas in the culture device, and the structure is used for simulating the physical constraint and chemical microenvironment of a root system in real soil and collecting secreta gas of the root system and gas generated by activity of soil microorganisms; the limitation that a traditional device can only collect or release liquid secretions is solved.

Description

Technical Field

[0001] This utility model relates to the technical field of plant root system simulation devices, and more specifically, to a device for simulating root secretion and soil gas collection. Background Technology

[0002] The interaction between plant roots and their surrounding environment is a key area of ​​research in ecology, agricultural science, and environmental science; plants release various organic and inorganic compounds into the soil through their roots, which are collectively referred to as root exudates.

[0003] Traditional root exudate research devices (such as hydroponic systems) have the following drawbacks: 1. They cannot simulate the physical constraints and chemical microenvironment of roots in real soil, resulting in distorted exudate release patterns; 2. Existing gas collection technologies cannot collect both root exudate gases and gases produced by soil microbial activities, neglecting the coupling relationship between root exudates and soil gas emissions (such as CO2, CH4, N2O), making it difficult to analyze the entire pathway of the rhizosphere carbon and nitrogen cycle.

[0004] Therefore, a device simulating root secretion and soil gas collection is developed to solve the above technical problems. Utility Model Content

[0005] In view of the above problems, this utility model provides a device for simulating root secretion and soil gas collection, which is used to simulate the physical constraints and chemical microenvironment of roots in real soil, simulate the release mode of root secretions in soil, and is equipped with a gas collection device for collecting the gas emitted from the soil.

[0006] To achieve the above objectives, this utility model provides a device for simulating root secretion and soil gas collection, comprising:

[0007] A cultivation device containing soil;

[0008] A gas collection device having an opening at its bottom;

[0009] The base is sealed to the bottom of the gas collection device;

[0010] The cultivation device is equipped with a root simulation unit, at least a portion of which is buried in the soil, and the root simulation unit secretes secretions into the soil.

[0011] The gas collection device is attached to the culture device through an opening, and the top of the gas collection device is provided with a gas collection channel for collecting gas inside the culture device.

[0012] As a further aspect of this utility model: the gas extraction channel includes:

[0013] A three-way valve and a vent pipe are provided. The three-way valve is located at the top of the gas collection device, and the vent pipe is located inside the gas collection device. The three-way valve is connected to the vent pipe to collect the gas released by the culture device.

[0014] As a further aspect of this utility model: the root system simulation unit includes:

[0015] The device comprises a syringe, a connecting tube, and a simulated root system. One end of the syringe is connected to the connecting tube, and the other end of the connecting tube is connected to the simulated root system. The syringe injects secretions into the simulated root system through the connecting tube.

[0016] As a further aspect of this invention: the simulated root system has multiple micropore structures for uniform diffusion of secretions.

[0017] As a further embodiment of this utility model: the top of the cultivation device is open, and the cultivation device has a receiving groove extending downward from the top for holding soil and water.

[0018] As a further embodiment of this utility model: a water tank is provided on the top of the base, and the gas collection device is fastened to the culture device and connected to the base through a water tank water seal.

[0019] As a further embodiment of this utility model: the height of the culture device is set to 14cm, the width to 10cm, and the thickness to 0.8cm.

[0020] As a further embodiment of this utility model: the height of the gas collection device is set to 15cm, the width to 12cm, and the thickness to 1cm.

[0021] As a further embodiment of this utility model: the base is 17cm long, 4cm wide, and 4cm high.

[0022] As a further aspect of this invention, the culture device is made of a transparent material.

[0023] Technical effects of this utility model:

[0024] 1. This application solves the limitation of traditional devices that can only collect or release liquid secretions by setting up a root simulation unit in the cultivation device to simulate the secretion of roots into the soil in the cultivation device and collecting the secretion gas and the gas generated by soil microbial activities through a gas collection device.

[0025] 2. This application collects data on changes in gas concentration within the culture device using a gas collection device, which is then used to comprehensively analyze the coupling relationship between root exudates and soil gas emissions, providing multi-dimensional data support for the study of rhizosphere carbon and nitrogen cycling.

[0026] 3. This application is approved. The gas collection channel set on the top of the gas collection device directly collects the gas generated by the cultivation device, achieving gas collection in a non-destructive manner, avoiding experimental interference, ensuring the stability of soil physicochemical properties during the experiment, and improving the reliability and repeatability of experimental results.

[0027] 4. This application is approved. The root system simulation unit controls the type and concentration of root exudates, simulates the dynamic changes of real root exudates, and simulates the physical constraints and chemical microenvironment of roots in real soil. It can more realistically reflect the diffusion and distribution process of root exudates in the soil, and provide reliable data for studying the impact of root exudates on soil microbial activity and nutrient cycling. Attached Figure Description

[0028] The accompanying drawings are provided to further illustrate the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention, but do not constitute a limitation thereof. In the drawings:

[0029] Figure 1 This is a schematic diagram of the overall structure of the device for simulating root secretion and soil gas collection described in this utility model.

[0030] The attached figures are labeled as follows:

[0031] 1. Cultivation apparatus; 101. Reservoir;

[0032] 11. Root system simulation unit; 111. Syringe; 112. Connecting tube; 113. Simulated root system; 1131. Microporous structure;

[0033] 2. Gas collection device;

[0034] 3. Base; 31. Water tank;

[0035] 4. Gas intake channel; 41. Three-way valve; 42. Gas inlet pipe. Detailed Implementation

[0036] In the following description, only certain exemplary embodiments are depicted simply. As will be appreciated by those skilled in the art, the described embodiments can be modified in various ways without departing from the spirit or scope of this invention. Therefore, the drawings and description are considered exemplary in nature and not restrictive.

[0037] The preferred embodiments of the present invention will be described below with reference to the accompanying drawings. It should be understood that the preferred embodiments described herein are for illustration and explanation only and are not intended to limit the present invention.

[0038] Please see Figure 1This invention illustrates a specific embodiment of a device for simulating root exudation and soil gas collection. This device can precisely control the type and concentration of root exudates, simulate the dynamic changes of root exudates under natural conditions, and collect soil emissions through a matching gas collection system. It comprehensively simulates the characteristics of the root microenvironment under natural conditions and the correlation mechanism of soil emissions, thus solving the defects of traditional root exudate research devices.

[0039] like Figure 1 As shown, an apparatus for simulating root secretion and soil gas collection includes a cultivation device 1, which is filled with soil, and the soil can contain any amount of water; the cultivation device 1 is provided with root simulation units 11, at least part of which are buried in the soil, and the root simulation units 11 discharge secretions into the soil to simulate the dynamic secretion process of root secretions in a real soil environment.

[0040] Furthermore, the device for simulating root secretion and soil gas collection also includes a gas collection device 2 with an opening at the bottom. The gas collection device 2 is fastened to the cultivation device 1 through the opening, and the bottom opening of the gas collection device 2 is sealed to the base 3. The top of the gas collection device 2 is provided with a gas collection channel 4 for collecting gas inside the cultivation device 1.

[0041] Understandably, the simulated root system 113 unit discharges secretions into the soil of the cultivation device 1. The soil gas generated by the coupling relationship between the secretion gas and the gases produced by soil microbial activities (such as CO₂, CH₄, N₂O) is collected by the gas collection device 2 and then collected to the outside through the gas collection channel 4 for experimental analysis. This is used to analyze the entire process of the rhizosphere carbon and nitrogen cycle. This solves the problem that traditional devices can only collect or release liquid secretions, cannot collect both root secretion gases and gases produced by soil microbial activities, ignore the coupling relationship between root secretions and soil gas emissions, and are difficult to analyze the entire process of the rhizosphere carbon and nitrogen cycle.

[0042] Specifically, such as Figure 1 As shown, the gas collection channel 4 includes a three-way valve 41 and a vent pipe 42. The three-way valve 41 is located at the top of the gas collection device 2, and the vent pipe 42 is located inside the gas collection device 2. The three-way valve 41 is connected to the vent pipe 42 to collect the gas released by the culture device 1.

[0043] It should be noted that this application controls the gas flow rate by setting a three-way valve 41. When the flow area between one valve core and the valve seat of the three-way valve 41 increases, the flow area between the other valve core and the valve seat decreases, thereby precisely controlling the amount of soil gas collected and comprehensively simulating the characteristics of the rhizosphere microenvironment under natural conditions and the correlation mechanism of soil gas emissions.

[0044] Specifically, such as Figure 1 As shown, the root simulation unit 11 includes a syringe 111, a connecting tube 112, and a simulated root system 113. The syringe 111 is connected to the connecting tube 112, and the connecting tube 112 is connected to the simulated root system 113. The syringe 111 injects secretions into the simulated root system 113 through the connecting tube 112. The amount of secretions injected is precisely controlled by the syringe 111. The connecting tube 112 is directly connected to the top of the simulated root system 113 to transfer the secretions into the simulated root system 113. The simulated root system 113 has multiple microporous structures 1131 to evenly diffuse the secretions into the soil.

[0045] Understandably, the syringe 111 may have graduations to precisely control the injection volume of liquid root secretions, fully simulating the release pattern of root secretions under natural conditions.

[0046] Specifically, the cultivation device 1 is made of transparent material, with an opening at the top and a receiving groove 101 extending downwards from the top for holding soil and water. Water is injected into the receiving groove 101, and the water level changes can be seen directly through the transparent cultivation device 1.

[0047] Preferably, the gas collection device 2 can also be made of transparent material, preferably polycarbonate or glass with high light transmittance and chemical inertness, to avoid interfering with the gas composition and not affect the observation of the culture device 1.

[0048] Preferably, such as Figure 1 As shown, a water tank 31 is provided on the top of the base 3, and the gas collection device 2 is set as a square cover structure. The gas collection device 2 is fastened to the culture device 1 and connected to the base 3 through the water tank 31 to form a gas-sealed environment, which prevents other substances from affecting the experimental results. It is low in cost and more convenient to implement.

[0049] Understandably, the height of the culture device 1 is set to 14cm, the width to 10cm, and the thickness to 0.8cm. The height of the gas collection device 2 is set to 15cm, the width to 12cm, and the thickness to 1cm, which can completely cover the transparent culture device 1. The length of the base 3 is set to 17cm, the width to 4cm, and the height to 4cm. The base 3 has sufficient water filling space and can be used with the gas collection device 2 to form a sealed environment.

[0050] Although exemplary embodiments of the present invention have been shown in the foregoing disclosure, it should be noted that various changes and modifications may be made without departing from the scope defined by the claims. Furthermore, while the elements of the present invention may be described or claimed individually, it is also contemplated that multiple elements may be included, unless explicitly limited to a single element.

[0051] Finally, it should be noted that the above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. An apparatus for simulating root exudation and soil gas collection, characterized by, include: A cultivation device containing soil; A gas collection device having an opening at its bottom; A base, which is connected to the bottom of the gas collecting device; The cultivation device is equipped with a root simulation unit, at least a portion of which is buried in the soil, and the root simulation unit discharges secretions into the soil. The gas collection device is attached to the culture device through an opening, and the top of the gas collection device is provided with a gas collection channel for collecting gas inside the culture device.

2. The apparatus of claim 1, wherein, The gas extraction channel includes: A three-way valve and a vent pipe are provided. The three-way valve is located at the top of the gas collection device, and the vent pipe is located inside the gas collection device. The three-way valve is connected to the vent pipe to collect the gas released by the culture device.

3. The apparatus of claim 1, wherein, The root system simulation unit includes: The device comprises a syringe, a connecting tube, and a simulated root system. One end of the syringe is connected to the connecting tube, and the other end of the connecting tube is connected to the simulated root system. The syringe injects secretions into the simulated root system through the connecting tube.

4. The apparatus of claim 3, wherein, The simulated root system has multiple micropore structures to facilitate the uniform diffusion of secretions.

5. The apparatus of claim 1, wherein, The cultivation device has an opening at the top and a receiving groove extending downwards from the top to hold soil and water.

6. The apparatus of claim 1, wherein, The base has a water tank at the top, and the gas collection device is configured as a cover structure. The gas collection device is fastened to the culture device and is sealed to the base through the water tank.

7. The apparatus for simulating root exudation and soil gas collection of any one of claims 1 to 6, wherein, The height of the culture device is set to 14cm, the width to 10cm, and the thickness to 0.8cm.

8. The apparatus for simulating root exudation and soil gas collection of any one of claims 1 to 6, wherein, The gas collection device is set to a height of 15cm, a width of 12cm, and a thickness of 1cm.

9. The apparatus for simulating root exudation and soil gas collection of any one of claims 1 to 6, wherein, The base is 17cm long, 4cm wide, and 4cm thick.

10. The apparatus for simulating root exudation and soil gas collection of any one of claims 1 to 6, wherein, The culture device is made of transparent material.

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