Portable device for collecting thrips and determining resistance

Abstract

The utility model discloses a portable device for collecting thrips and determining resistance, and belongs to the technical field of insect bioassay. The device comprises an air exhaust gun, a cryopreservation pipe and an insect suction pipe, the insect suction pipe is connected with a fixing block at the bottom of the cryopreservation pipe through a limiting block, a filter screen is arranged at the bottom of the cryopreservation pipe, an annular partition plate is arranged on the upper portion in the cryopreservation pipe, the middle of the annular partition plate is connected with a blocking piece through a torsional spring, and the blocking piece can only be opened towards the interior of the cryopreservation pipe; an opening with the diameter matched with the outer diameter of the insect suction pipe is formed in the middle of the cryopreservation pipe cover, and the insect suction pipe is longer than the cryopreservation pipe and arranged in the cryopreservation pipe; the bottom of the cryopreservation pipe is connected with an air exhaust gun through a rubber sleeve head and an air pipe. The device is easy to assemble and convenient to carry, different medicaments are tested by replacing the cryopreservation tube, the effect of comprehensive and accurate determination is achieved, insect suction and testing are completed in the same device, transfer is not needed, manual errors are reduced, and a more accurate determination result is obtained.

Description

Technical Field

[0001] This utility model belongs to the field of insect biological assay technology, specifically relating to a portable device for thrips collection and resistance assay. Background Technology

[0002] Thrips are major pests on a variety of crops, including cotton, vegetables, and flowers. They are characterized by their small size, rapid reproduction, strong concealment, and wide host range. They are also highly susceptible to developing resistance to pesticides, which makes control extremely difficult and seriously affects the quality of crops and export trade.

[0003] Whether pests have developed pesticide resistance is generally measured through bioassay results. Knowing the level of pesticide resistance provides an important theoretical basis for integrated pest management in the field. Thrips are small and adept at flight; existing bioassay devices have the following limitations in collecting and measuring thrips:

[0004] First, the bioassay apparatus is complex and inconvenient to carry. Second, while the agents are primarily contact-based stomach poisons, the toxicity assay apparatus still biases towards stomach poisons, resulting in incomplete and inaccurate measurements. Finally, the transfer of thrips to the apparatus involves significant human error and is complex, making it difficult to investigate the results. Utility Model Content

[0005] This invention proposes a portable device for thrips collection and resistance determination, which is easy to carry and provides accurate measurements, thus overcoming the shortcomings of the prior art.

[0006] A portable device for thrips collection and resistance testing, characterized by comprising an air extraction gun, a cryopreservation tube, and a suction tube. Symmetrical limiting blocks are provided on the outer side of the bottom of the suction tube. A fixing block is provided at the bottom of the cryopreservation tube. A through hole extending to the bottom of the cryopreservation tube is provided in the center of the fixing block, and the diameter of the through hole matches the outer diameter of the suction tube. A horizontal annular groove is provided on the side of the through hole, and a vertical rectangular groove matching the limiting block is provided on the top surface of the through hole. After the limiting block is inserted into the rectangular groove and then screwed into the annular groove, the suction tube is fixed within the fixing block. A filter screen is installed at the bottom of the through hole; an annular baffle is installed in the upper part of the cryopreservation tube, the inner diameter of which is greater than the total length of the cryopreservation tube and the limiting block, and a baffle with a diameter not less than the inner diameter of the annular baffle is installed in the middle of the annular baffle. The baffle is connected to the annular baffle by a torsion spring and can only be opened to the inside of the cryopreservation tube; an opening with a diameter matching the outer diameter of the spirochete is opened in the middle of the cryopreservation tube cap, the length of which is greater than the length of the cryopreservation tube and is installed inside the cryopreservation tube; the bottom of the cryopreservation tube is connected to the suction gun through a rubber sleeve and an air tube.

[0007] The beneficial effects of this invention are as follows: After the cryopreservation tube is treated by immersion, insecticide remains on the inner wall of the tube. The suction tube is inserted from bottom to top through the bottom of the cryopreservation tube cap. The limiting block prevents the suction tube from falling off the cap. The cap is then installed on the cryopreservation tube. The suction tube is pushed into the fixing block at the bottom of the cryopreservation tube and rotated to secure it. During the pushing process, the baffle is opened. The suction tube opening is aligned with the thrips, and the thrips are sucked into the bottom of the suction tube using an air suction gun. The thrips are blocked by the filter screen to prevent them from being sucked into the trachea. After suctioning, the suction tube is rotated to move the limiting block to the rectangular groove. The suction tube is pulled out until the limiting block is blocked by the cryopreservation tube cap. After the suction tube is pulled out, the baffle springs back to its original position under the action of a torsion spring, creating a closed space inside the cryopreservation tube for observing and measuring the thrips' resistance to the pesticide. This device is simple to assemble and easy to carry. Different agents can be tested by changing cryovials, achieving comprehensive and accurate results. Furthermore, the trematode and testing are completed within the same device, eliminating the need for transfer, reducing human error, and obtaining more accurate test results. Attached Figure Description

[0008] Figure 1 This is a schematic diagram of a portable device for thrips collection and resistance determination.

[0009] Figure 2 This is a top view of the positioning block.

[0010] Among them: 1-Ejector gun, 11-Rubber sleeve, 12-Air tube, 2-Cryopreservation tube, 21-Cryopreservation tube cap, 3-Insect suction tube, 4-Limiting block, 5-Fixing block, 51-Annular groove, 52-Rectangular groove, 6-Filter screen, 7-Annular partition, 8-Baffle plate. Detailed Implementation

[0011] Example 1: A portable device for thrips collection and resistance determination includes an air extraction gun 1, a cryopreservation tube 2, and a suction tube 3. A limiting block 4 is symmetrically arranged on the outer side of the bottom of the suction tube 3. A fixing block 5 is provided at the bottom of the cryopreservation tube 2. A through hole extending to the bottom of the cryopreservation tube is provided in the middle of the fixing block 5, and the diameter of the through hole matches the outer diameter of the suction tube 3. A horizontal annular groove 51 is provided on the side of the through hole, and a vertical rectangular groove 52 matching the limiting block 4 is provided on the top surface of the through hole. After the limiting block 4 is inserted into the rectangular groove 52 and screwed into the annular groove 51, the suction tube 3 is fixed inside the fixing block 5. A filter screen 6 is provided at the bottom of the through hole. An annular partition 7 is provided in the upper part of the cryopreservation tube 2. The inner ring diameter of the annular partition 7 is greater than the total length of the cryopreservation tube 2 and the limiting block 4. A baffle 8 with a diameter not less than the inner ring diameter of the annular partition 7 is provided in the middle of the annular partition 7. The baffle 8 is connected to the annular partition 7 by a torsion spring, and the baffle 7 can only be opened into the cryopreservation tube 2. The cryopreservation tube cap 21 has an opening in the middle with a diameter matching the outer diameter of the siphon tube 3. The length of the siphon tube 3 is greater than the length of the cryopreservation tube 2 and is set inside the cryopreservation tube 2. The bottom of the cryopreservation tube 2 is connected to the suction gun 1 through the rubber sleeve 11 and the air tube 12.

[0012] In use, after treating the cryopreservation tube 2 with an immersion method, insecticide is left on the inner wall of the tube. The suction tube 3 is inserted from bottom to top through the bottom of the cryopreservation tube cap 21. The limiting block 4 prevents the suction tube 3 from falling off the cryopreservation tube cap 21. The cryopreservation tube cap 21 is installed on the cryopreservation tube 2. The suction tube 3 is pushed into the fixing block 5 at the bottom of the cryopreservation tube 2 and rotated to fix it. During the pushing process, the baffle 8 is opened. The mouth of the suction tube 3 is aligned with the thrips, and the suction gun 1 is used to suck the thrips into the bottom of the suction tube 3. The thrips are blocked by the filter screen 6 to prevent them from being sucked into the trachea 12. After suctioning, the suction tube 3 is rotated to move the limiting block 4 to the rectangular groove 52. The suction tube 3 is pulled out until the limiting block 4 is blocked by the cryopreservation tube cap 21. After the suction tube 3 is pulled out, the baffle 8 springs back to its original position under the action of the torsion spring, forming a closed space inside the cryopreservation tube 2 for observing and measuring the thrips' resistance to the pesticide.

Claims

1. A portable device for thrips collection and resistance determination, characterized in that... The system includes an air extraction gun, a cryopreservation tube, and a suction tube. Symmetrical limiting blocks are located on the outer bottom of the suction tube. A fixing block is located at the bottom of the cryopreservation tube. A through-hole, with a diameter matching the outer diameter of the suction tube, is located in the center of the fixing block. A horizontal annular groove is located on the side of the through-hole, and a vertical rectangular groove matching the limiting block is located on the top surface of the through-hole. After the limiting block is inserted into the rectangular groove and screwed into the annular groove, it fixes the suction tube within the fixing block. A filter screen is located at the bottom of the through-hole. An annular partition is located at the upper part of the cryopreservation tube. The inner diameter of the partition is greater than the total length of the cryopreservation tube and the limiting block. A baffle with a diameter not less than the inner diameter of the partition is located in the center of the partition. The baffle is connected to the partition via a torsion spring and can only open into the cryopreservation tube. An opening matching the outer diameter of the suction tube is located in the center of the cryopreservation tube cap. The suction tube, longer than the cryopreservation tube, is located inside the cryopreservation tube. The bottom of the cryopreservation tube is connected to the air extraction gun via a rubber sleeve and an air tube.

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